Gastric Electrical Pacing Reduces Apoptosis of Interstitial Cells of Cajal via Antioxidative Stress Effect Attributing to Phenotypic Polarization of M2 Macrophages in Diabetic Rats

Background. Gastric electrical pacing (GEP) could restore interstitial cells of Cajal in diabetic rats. M2 macrophages contribute to the repair of interstitial cells of Cajal injury though secreting heme oxygenase-1 (HO-1). The aim of the study is to investigate the effects and mechanisms of gastric electrical pacing on M2 macrophages in diabetic models. Methods. Sixty male Sprague-Dawley rats were randomized into control, diabetic (DM), diabetic with the sham GEP (DM+SGEP), diabetic with GEP1 (5.5 cpm, 100 ms, 4 mA) (DM+GEP1), diabetic with GEP2 (5.5 cpm, 300 ms, 4 mA) (DM+GEP2), and diabetic with GEP3 (5.5 cpm, 550 ms, 4 mA) (DM+GEP3) groups. The apoptosis of interstitial cells of Cajal and the expression of macrophages were detected by immunofluorescence technique. The expression levels of the Nrf2/HO-1 and NF-κB pathway were evaluated using western blot analysis or immunohistochemical method. Malonaldehyde, superoxide dismutase, and reactive oxygen species were tested to reflect the level of oxidative stress. Results. Apoptosis of interstitial cells of Cajal was increased in the DM group but significantly decreased in the DM+GEP groups. The total number of macrophages was almost the same in each group. In the DM group, M1 macrophages were increased and M2 macrophages were decreased. However, M2 macrophages were dramatically increased and M1 macrophages were reduced in the DM+GEP groups. Gastric electrical pacing improved the Nrf2/HO-1 pathway and downregulated the phosphorylation of NF-κB. In the DM group, the levels of malonaldehyde and reactive oxygen species were elevated and superoxide dismutase was lowered, while gastric electrical pacing reduced the levels of malonaldehyde and reactive oxygen species and improved superoxide dismutase. Conclusion. Gastric electrical pacing reduces apoptosis of interstitial cells of Cajal though promoting M2 macrophages polarization to play an antioxidative stress effect in diabetic rats, which associates with the activated Nrf2/HO-1 pathway and the phosphorylation of NF-κB pathway.

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Engineering of a novel chimera of superoxide dismutase and Vitreoscilla hemoglobin for rapid detoxification of reactive oxygen species

Journal of Bioscience and Bioengineering ◽

10.1016/j.jbiosc.2010.07.001 ◽

2010 ◽

Vol 110 (6) ◽

pp. 633-637 ◽

Cited By ~ 4

Author(s):

Chartchalerm Isarankura-Na-Ayudhya ◽

Sakda Yainoy ◽

Tanawut Tantimongcolwat ◽

Leif Bülow ◽

Virapong Prachayasittikul

Keyword(s):

Reactive Oxygen Species ◽

Superoxide Dismutase ◽

Reactive Oxygen ◽

Vitreoscilla Hemoglobin ◽

Oxygen Species

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Effects of Superoxide Dismutase Inhibitors and Glucose on Cell Death and Generation of Reactive Oxygen Species in Pea Leaves

Biochemistry (Moscow) ◽

10.1134/s0006297921070087 ◽

2021 ◽

Vol 86 (7) ◽

pp. 878-886

Author(s):

Vitaly D. Samuilov ◽

Dmitry B. Kiselevsky ◽

Elena V. Dzyubinskaya ◽

Olga Yu. Frolova

Keyword(s):

Reactive Oxygen Species ◽

Cell Death ◽

Superoxide Dismutase ◽

Reactive Oxygen ◽

Oxygen Species

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S-allyl cysteine restores erectile function through inhibition of reactive oxygen species generation in diabetic rats

Andrology ◽

10.1111/j.2047-2927.2012.00060.x ◽

2013 ◽

Vol 1 (3) ◽

pp. 487-494 ◽

Cited By ~ 46

Author(s):

J. Yang ◽

T. Wang ◽

J. Yang ◽

K. Rao ◽

Y. Zhan ◽

...

Keyword(s):

Reactive Oxygen Species ◽

Erectile Function ◽

Reactive Oxygen Species Generation ◽

Reactive Oxygen ◽

Diabetic Rats ◽

Oxygen Species

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Efek Ekstrak Etanol Daun Gedi Merah (Abelmoschus Manihot (L.) Medik) Terhadap Kadar Superoxide Dismutase Dan Malondialdehyde Jaringan Ginjal Tikus Model Diabetes Melitus Tipe 2

Jurnal Kesehatan Islam : Islamic Health Journal ◽

10.33474/jki.v8i2.8889 ◽

2020 ◽

Vol 8 (2) ◽

pp. 83

Author(s):

Ahmad Fuadi ◽

Yoyon Arif ◽

Yudi Purnomo

Keyword(s):

Reactive Oxygen Species ◽

Superoxide Dismutase ◽

Multiple Dose ◽

Reactive Oxygen ◽

Oxygen Species ◽

Sprague Dawley ◽

Per Oral ◽

One Way Anova ◽

Diabetes Melitus

Hiperglikemia pada Diabetes Melitus (DM) meningkatkan produksi Reactive Oxygen Species (ROS) dan berperan terhadap risiko komplikasi nefropati diabetik. Daun gedi merah (Abelmoschus manihot (L.) Medik) berkhasiat sebagai antidiabetik dan antioksidan tetapi penelitian ekstrak etanol daun gedi merah (EEDGM) untuk mencegah nefropati diabetik belum banyak dilaporkan. Penelitian ini bertujuan untuk mengetahui efek EEDGM terhadap kadar SOD dan MDA ginjal tikus model DM.Metode: Tikus Sprague dawley jantan usia 4-6 minggu dikelompokan menjadi 2 kelompok kontrol dan 3 kelompok perlakuan (n=25 ekor). Tikus DM dibuat dengan diet tinggi lemak-fruktosa (DTLF) dan streptozotocin (STZ) 25 mg/kgBB i.p multiple dose. Ekstrak etanol daun gedi merah (EEDGM) diberikan per oral selama 4 minggu. Kadar SOD dan MDA ginjal diukur menggunakan SOD rat kit dan MDA rat kit. Hasil dianalisa dengan One Way Anova dilanjutkan dengan uji BNT (p<0,05).Hasil: Pemberian EEDGM dosis 800 mg/kgBB menghambat penurunan kadar SOD jaringan ginjal dengan persentase sekitar 60% dibandingkan KDM (p<0,05). Pemberian EEDGM dosis 400 mg/kgBB menghambat peningkatan kadar MDA jaringan ginjal dengan persentase sekitar 20% dibandingkan KDM (p<0,05). Induksi DTLF dan STZ menurunkan kadar SOD jaringan ginjal dengan persentase sekitar 40% dan meningkatkan kadar MDA jaringan ginjal dengan persentase sekitar 30%.Kesimpulan: Pemberian EEDGM dapat menghambat penurunan kadar SOD dan peningkatan kadar MDA jaringan ginjal tikus model DM.

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Detection of oxidative stress induced by calcium phosphate bions in human arterial endothelial cells

ZHurnal «Patologicheskaia fiziologiia i eksperimental`naia terapiia» ◽

10.25557/0031-2991.2021.01.70-78 ◽

2021 ◽

pp. 70-78

Author(s):

А.Г. Кутихин ◽

Д.К. Шишкова ◽

Р.А. Мухамадияров ◽

Е.А. Великанова

Keyword(s):

Oxidative Stress ◽

Reactive Oxygen Species ◽

Endothelial Cells ◽

Cell Death ◽

Superoxide Dismutase ◽

Calcium Phosphate ◽

Reactive Oxygen ◽

Oxygen Species ◽

Bafilomycin A1 ◽

Arterial Endothelial Cells

Введение. Кальций-фосфатные бионы (КФБ) формируются в организме человека при перенасыщении сыворотки ионами кальция и фосфора и вызывают дисфункцию эндотелия, однако молекулярные механизмы нарушения функционирования эндотелия при воздействии КФБ не ясны. Цель исследования - выяснение роли кальций-фосфатных бионов различной формы в развитии окислительного стресса в артериальных эндотелиальных клетках (ЭК) человека. Методика. Для детекции окислительного стресса к конфлюэнтным культурам первичных ЭК коронарной и внутренней грудной артерии человека добавляли равные концентрации КФБ сферической или игольчатой формы (СКФБ и ИКФБ соответственно) с последующим культивированием в течение 1 и 4 ч, добавлением флюоресцентных индикаторов окислительного стресса MitoSOX Red и CellROX Green и конфокальной микроскопией. Измеряли концентрацию продуктов перекисного окисления липидов в культуральной жидкости через 24 ч экспозиции эндотелиальных клеток КФБ. Анализ нейтрализации цитотоксических эффектов перекисного окисления липидов проводили путем добавления к ЭК супероксиддисмутазы и каталазы на 4 или 24 ч (одновременно с КФБ). Для сравнения механизмов клеточной гибели при воздействии СКФБ и ИКФБ анализировали цитотоксичность обоих типов бионов при одновременном воздействии лизосомального ингибитора бафиломицина А1. Результаты. Значимого увеличения генерации активных форм кислорода (АФК) в результате экспозиции СКФБ (независимо от линии ЭК и продолжительности экспозиции) не было выявлено. В то же время наблюдалось повышение генерации супероксида через 4 ч, а иных свободных радикалов через 1 ч после добавления ИКФБ к ЭК. Предварительная нейтрализация АФК супероксиддисмутазой и каталазой частично защищала ЭК от индуцируемой ИКФБ гибели. При этом добавление бафиломицина А1 к ЭК частично защищало их от гибели только при воздействии СКФБ, но не ИКФБ. Заключение. Гибель ЭК при воздействии СКФБ происходит в результате первичного повреждения лизосом, а при воздействии ИКФБ - в первую очередь вследствие окислительного стресса. Background. Calcium phosphate bions (CPB) form in the human blood upon its supersaturation with calcium and phosphate and provoke endothelial dysfunction; however, the molecular mechanisms of these pathological processes remain unclear. Aim. To elucidate the role of differently shaped CPBs in induction of oxidative stress in human arterial endothelial cells (Ecs). Methods. For detection of oxidative stress, equal concentrations of spherical CPB (CPB-S) or needle-shaped CPB (CPB-N) were added to confluent cultures of primary human coronary artery and internal thoracic artery ECs for 1 and 4 h; this was followed by MitoSOX Red and CellROX Green staining and subsequent confocal microscopy. Concentration of thiobarbituric acid-reactive substances was measured in the EC culture supernatant at 24 h of the CPB exposure. The lipid peroxidation cytotoxicity was neutralized by adding superoxide dismutase and catalase to ECs for 4 or 24 h. To compare cell death subroutines induced by CPB-S and CPB-N, the effect of bafilomycin A1, a lysosomal inhibitor, on CRB cytotoxicity was studied. Results. No increase in reactive oxygen species generation was observed in the CPB-S exposure, regardless of the EC line and exposure duration. However, addition of CPB-N to ECs increased the production of superoxide and other free radicals after four- and one-hour exposure, respectively. Prior neutralization of reactive oxygen species with superoxide dismutase and catalase partially protected ECs from CPB-N- but not CPB-S-induced death while bafilomycin A1, vice versa, protected ECs from CPB-S- but not CPB-N-induced death. Conclusion. CPB-S cause cell death due to primary damage of lysosomes whereas CPB-N induce apoptosis due to oxidative stress.

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Potentiation of Antibiofilm Activity of Amphotericin B by Superoxide Dismutase Inhibition

Oxidative Medicine and Cellular Longevity ◽

10.1155/2013/704654 ◽

2013 ◽

Vol 2013 ◽

pp. 1-7 ◽

Cited By ~ 12

Author(s):

Katrijn De Brucker ◽

Anna Bink ◽

Els Meert ◽

Bruno P. A. Cammue ◽

Karin Thevissen

Keyword(s):

Reactive Oxygen Species ◽

Superoxide Dismutase ◽

Candida Albicans ◽

Amphotericin B ◽

Reactive Oxygen ◽

Intracellular Reactive Oxygen Species ◽

Superoxide Dismutases ◽

Antibiofilm Activity ◽

Oxygen Species ◽

Ammonium Tetrathiomolybdate

This study demonstrates a role for superoxide dismutases (Sods) in governing tolerance ofCandida albicansbiofilms to amphotericin B (AmB). Coincubation ofC. albicansbiofilms with AmB and the Sod inhibitors N,N′-diethyldithiocarbamate (DDC) or ammonium tetrathiomolybdate (ATM) resulted in reduced viable biofilm cells and increased intracellular reactive oxygen species levels as compared to incubation of biofilm cells with AmB, DDC, or ATM alone. Hence, Sod inhibitors can be used to potentiate the activity of AmB againstC. albicansbiofilms.

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