scholarly journals Cytological Effects of Serum Isolated from Polytraumatized Patients on Human Bone Marrow-Derived Mesenchymal Stem Cells

2021 ◽  
Vol 2021 ◽  
pp. 1-18
Author(s):  
Yazhou Long ◽  
Katrin Bundkirchen ◽  
Pascal Gräff ◽  
Christian Krettek ◽  
Sandra Noack ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
Damage Control ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Damage Control Orthopedics ◽  
Proliferation Capacity ◽  
Healthy Donors

Due to their immunomodulatory and regenerative capacity, human bone marrow-derived mesenchymal stem cells (hBMSCs) are promising in the treatment of patients suffering from polytrauma. However, few studies look at the effects of sera from polytraumatized patients on hBMSCs. The aim of this study was to explore changes in hBMSC properties in response to serum from polytrauma patients taken at different time points after the trauma incident. For this, sera from 84 patients with polytrauma (collected between 2010 and 2020 in our department) were used. In order to test the differential influence on hBMSC, sera from the 1st (D1), 5th (D5), and 10th day (D10) after polytrauma were pooled, respectively. As a control, sera from three healthy donors (HS), matched with respect to age and gender to the polytrauma group, were collected. Furthermore, hBMSCs from four healthy donors were used in the experiments. The pooled sera of HS, D1, D5, and D10 were analyzed by multicytokine array for pro-/anti-inflammatory cytokines. Furthermore, the influence of the different sera on hBMSCs with respect to cell proliferation, colony forming unit-fibroblast (CFU-F) assay, cell viability, cytotoxicity, cell migration, and osteogenic and chondrogenic differentiation was analyzed. The results showed that D5 serum significantly reduced hBMSC cell proliferation capacity compared with HS and increased the proportion of dead cells compared with D1. However, the frequency of CFU-F was not reduced in polytrauma groups compared with HS, as well as the other parameters. The serological effect of polytrauma on hBMSCs was related to the time after trauma. It is disadvantageous to use BMSCs in polytraumatized patients at least until the fifth day after polytrauma as obvious cytological changes could be found at that time point. However, it is promising to use hBMSCs to treat polytrauma after five days, combined with the concept of “Damage Control Orthopedics” (DCO).

Self – aligned mesoporous titania nanotubes – reduced graphene oxide hybrid surface: A potential scaffold for osteogenesis

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Shikha Wadhwa ◽  
Souradeep Roy ◽  
Neha Mittal ◽  
Alishba T John ◽  
Swati Midha ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
Graphene Oxide ◽  
Bone Cell ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Reduced Graphene ◽  
Marrow Mesenchymal Stem Cells

Abstract Damaged or degenerative joints are treated with total joint replacement, however, despite many successful outcomes, significant problems such as implant loosening, and failure occur. These problems occur due to the inability of the implant to support bone cell attachment, delaying the cell–implant interaction affecting bone regeneration. Hence it is recommended to develop and modify implants to induce bone cell proliferation. Herein, we report a self-aligned titania nanotubes–reduced graphene oxide (TiNT–reduced GO) hybrid surface for growth and proliferation of human bone marrow mesenchymal stem cells. The hybrid surface is characterised using electron microscopy, X-ray diffraction and Raman spectroscopy. The human bone marrow mesenchymal stem cell proliferation was studied using standard MTT and fluorescence assays. TiNT–reduced GO hybrid surface, with TiO2 nanotube of diameter 80 –110 nm and length ⁓500 μm with uniform deposition of reduced GO all over the surface, demonstrated about 50% increased cell growth compared to TiO2 nanotubes surface. The osteoinductive behaviour of TiNT–reduced GO is observed as these hybrid films support the proliferation of human bone marrow mesenchymal stem cells, hence promising to be a potential implant material for bone regeneration.

The immunosuppressive effects of human bone marrow-derived mesenchymal stem cells target T cell proliferation but not its effector function

2008 ◽  
Vol 251 (2) ◽  
pp. 131-136 ◽  
Author(s):  
Rajesh Ramasamy ◽  
Chih Kong Tong ◽  
Heng Fong Seow ◽  
Sharmili Vidyadaran ◽  
Francesco Dazzi
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Cell Proliferation ◽  
Mesenchymal Stem Cells ◽  
T Cell ◽  
Effector Function ◽  
Human Bone ◽  
Human Bone Marrow ◽  
T Cell Proliferation

scholarly journals Identity, proliferation capacity, genomic stability and novel senescence markers of mesenchymal stem cells isolated from low volume of human bone marrow

Oncotarget ◽  
2016 ◽  
Vol 7 (10) ◽  
pp. 10788-10802 ◽  
Author(s):  
Gabrielis Kundrotas ◽  
Evelina Gasperskaja ◽  
Grazina Slapsyte ◽  
Zivile Gudleviciene ◽  
Jan Krasko ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Genomic Stability ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Proliferation Capacity ◽  
Low Volume

Candidate kinases for adipogenesis and osteoblastogenesis from human bone marrow mesenchymal stem cells

2021 ◽  
Author(s):  
Xia Yi ◽  
Ping Wu ◽  
Jianyun Liu ◽  
Shan He ◽  
Ying Gong ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Transcription Factors ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Cytoplasmic Proteins ◽  
Marrow Mesenchymal Stem Cells

Adipogenesis and osteoblastogenesis (adipo-osteoblastogenesis) are closely related processes involving with the phosphorylation of numerous cytoplasmic proteins and key transcription factors.

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