Effect of Volume and Renewal of the Storage Media on the Release of Monomer from Dental Composites

This study evaluated the effect of the volume and renewing of storage media on monomer leachability from dental composite. Samples of two dental composites (BEAUTIFIL II Gingiva (BG) and Filtek Bulk-Fill Flowable (FBF)) were stored after polymerization in 1 and 3 milt storage media (ethanol/water 75%) for seven days. Refreshing of storage media was done in half of the samples of each group. The amounts of releasing monomers (UDMA, BisGMA, TEGDMA) in storage media were measured by high-performance liquid chromatography (HPLC). Data was analyzed using two-way ANOVA and t-test (α = 0.05). Elution of TEGDMA and UDMA from both composites was significantly higher in 3 mL storage media. In groups with refreshing of storage media, BisGMA had higher amounts of release. Saturation makes the storage media volume important factor in monomer elution. Refreshing of storage media had significant effect on monomer release before the elution of 50% of total released monomer.

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ANALISIS KADAR BENZOAT DAN SORBAT PADA SAUS SAMBAL KEMASAN YANG DIJUAL DI PASAR BARU BEKASI DENGAN METODE HPLC

Jurnal Mitra Kesehatan ◽

10.47522/jmk.v3i1.47 ◽

2020 ◽

Vol 3 (1) ◽

pp. 22-27

Author(s):

Maulidya Mitha Rianto ◽

Elfira Mayasari ◽

Siti Nurfajriah

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Performance ◽

Cross Sectional ◽

Hplc Data

Pendahuluan: Bahan Tambahan Pangan (BTP) digunakan secara luas oleh masyarakat. Bahan Tambahan Pangan (BTP) yang banyak digunakan dalam saus sambal adalah pengawet seperti benzoat dan sorbat. Penggunaan pengawet pada bahan pangan tidak boleh melebihi ambang batas yang sudah ditentukan karena dapat memberikan efek bagi kesehatan, seperti benzoat yang dapat menimbulkan reaksi alergi dan bersifat karsinogenik karena adanya benzene serta sorbat yang dapat menyebabkan mutasi gen bila dikombinasikan dengan asam askorbat. Penelitian ini bertujuan untuk mengetahui kadar benzoat dan sorbat pada saus sambal kemasan yang dijual di Pasar Baru Bekasi dan melihat kesesuaian kadar pengawet dengan ambang batas yang sudah ditentukan. Metode: Metode penelitian yang digunakan adalah deskriptif kuantitatif dengan pendekatan cross-sectional. Kadar benzoat dan sorbat diperiksa menggunakan metode High Performance Liquid Chromatography (HPLC). Data kuantitatif yang diperoleh dari hasil pengukuran dianalisis dengan membandingkan kadar maksimum benzoat dan sorbat yang diperbolehkan pada saus menurut Peraturan Kepala BPOM No. 36 Tahun 2013, yaitu 1000 mg/kg. Kadar benzoat dan sorbat yang melebihi ambang batas menandakan bahwa saus sambal tersebut tidak boleh untuk dikonsumsi. Hasil: Hasil penelitian menunjukkan bahwa seluruh 100% sampel mengandung benzoat melebihi kadar maksimum yang telah ditetapkan dan 50% sampel mengandung sorbat dengan kadar di bawah ambang batas yang telah ditentukan. Kesimpulan: Berdasarkan penelitian yang telah dilakukan, saran yang dapat diberikan untuk penelitian selanjutnya adalah melakukan analisis kadar benzoat dan sorbat pada sampel bahan pangan yang lain mengingat bahwa benzoat dan sorbat sering digunakan sebagai kombinasi pengawet dan diperbolehkan untuk digunakan dalam bahan pangan.

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Phytoplankton community during a coccolithophorid bloom in the Patagonian Shelf: microscopic and high-performance liquid chromatography pigment analyses

Journal of the Marine Biological Association of the United Kingdom ◽

10.1017/s0025315411000439 ◽

2011 ◽

Vol 92 (1) ◽

pp. 13-27 ◽

Cited By ~ 15

Author(s):

Márcio Silva de Souza ◽

Carlos Rafael Borges Mendes ◽

Virgínia Maria Tavano Garcia ◽

Ricardo Pollery ◽

Vanda Brotas

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Performance ◽

Phytoplankton Community ◽

Sampling Period ◽

Emiliania Huxleyi ◽

Patagonian Shelf ◽

Phaeocystis Antarctica ◽

Hplc Data ◽

Phytoplankton Group

We describe the phytoplankton community and biomass during a summer coccolithophorid bloom sampled over the Patagonian shelf (48.5°S–50.5°S). Those phytoplankton species can contribute to the flux of calcium carbonate out of surface waters. Results from both microscope and high-performance liquid chromatography (HPLC) analysis are shown to complement information on the phytoplankton community. From CHEMTAX analysis of HPLC data, the most important organisms and groups identified were the coccolithophorid Emiliania huxleyi, the haptophyte Phaeocystis antarctica, dinoflagellates, diatoms, cryptophytes, prasinophytes and cyanobacteria. Phytoplankton microscope counts were converted into phytoplankton group-specific biovolume estimates. Although some microscope-identified taxa could not be determined by CHEMTAX, e.g. the autotrophic ciliate Myrionecta rubra, cluster analyses from both techniques showed similar results for the main groups. Both Emiliania huxleyi cell concentration and biomass, and the pigment 19′-hexanoyloxyfucoxanthin were the most important biological features during the sampling period. At surface, nitrate was moderately high (0.2–4.2 µM) in coccolithophorid-dominated samples, whereas phosphate (<0.33 µM) and silicate (<1.35 µM) concentrations were low. Among the environmental factors low Si:N ratios were mainly associated with the dominance of E. huxleyi. Competition and probably differential grazing could also promote a coccolithophorid outgrowth over other photoautotrophs during the summer season in the Patagonian shelf.

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Bioakumulasi residu pestisida pada komunitas gastropoda di perairan Sungai Kalisat, Kabupaten Malang

DEPIK ◽

10.13170/depik.8.3.14368 ◽

2019 ◽

Vol 8 (3) ◽

pp. 167-175

Author(s):

Nanik Retno Buwono ◽

Trinita Gultom ◽

Setya Widi Ayuning ◽

Supriatna Supriatna

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Pesticide Residues ◽

High Performance ◽

Average Value ◽

Pesticide Pollution ◽

Pyrethroid Pesticides ◽

Carbamate Group ◽

The Eu ◽

Hplc Data

Abstract. Gastropods can respond to pesticide pollution in the environment because their life is relatively sedentary and their movements are very limited. The character of pesticides are not selective and persistent can result in bioaccumulation of pesticide content in gastropod. Hence, the aim of the study was to determine the levels of pesticide residues that accumulate in the gastropod body at Kalisat River, Malang Regency. The pesticide residues in gastropods was measured using High Performance Liquid Chromatography (HPLC). Data were analyzed descriptively comparative or compared with the quality standards which exceeded the maximum residual limit set by the EU Pesticide Database. The results showed that pyrethroid pesticides were found with the active ingredient beta-cyfluthrin (mean 0.05 mg/g) and carbamate group with three highest active ingredients namely propoxur (average value of 0.15 mg/g), carbofuran (average value of 0.16 mg/g) and carbaryl (average value of 0.11 mg/g). Bioaccumulation factor values at all stations are greater than 1 (BAF1) that indicates the gastropods can accumulate pesticide residues.Keywords: bioaccumulation, gastropod, pesticide residues, Kalisat River Abstrak. Gastropoda dapat memberikan respon terhadap pencemaran pestisida di lingkungan karena hidupnya relatif menetap dan pergerakannya sangat terbatas. Sifat pestisida yang tidak selektif dan persisten dapat mengakibatkan terjadinya bioakumulasi atau penumpukan kandungan pestisida dalam tubuh gastropoda. Penelitian ini bertujuan untuk mengetahui kadar residu pestisida yang terakumulasi dalam tubuh gastropoda di Sungai Kalisat, Kabupaten Malang. Pengukuran residu pestisida pada gastropoda menggunakan alat High Performance Liquid Chromatography (HPLC). Hasil pengukuran menunjukkan bahwa ditemukan pestisida golongan piretroid dengan bahan aktif jenis beta siflutrin (rerata 0,05 mg/g) dan golongan karbamat dengan tiga jenis bahan aktif tertinggi, yaitu propoksur (rerata 0,15 mg/g), karbofuran (rerata 0,16 mg/g) dan karbaril (rerata 0,11 mg/g). Data dianalisis secara deskriptif komparatif atau dibandingan dengan baku mutu EU Pesticide Database tahun 2019 telah melebihi batas maksimum residu yang ditetapkan oleh EU Pesticide Database. Nilai faktor bioakumulasi pada seluruh stasiun lebih besar dari 1 (BAF1) yang menunjukkan bahwa gastropoda mampu mengakumulasi residu pestisida. Pestisida dapat ditemukan pada gastropoda dengan melalui difusi langsung dari lingkungan dan juga melalui proses pencernaan gastropoda. Kata kunci: bioakumulasi, gastropoda, residu pestisida, Sungai Kalisat

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Identification and quantification of monomers released from dental composites using HPLC

Brazilian Archives of Biology and Technology ◽

10.1590/s1516-89132009000400009 ◽

2009 ◽

Vol 52 (4) ◽

pp. 855-862 ◽

Cited By ~ 7

Author(s):

Lucí Regina Panka Archegas ◽

Rodrigo Nunes Rached ◽

Sérgio Aparecido Ignacio ◽

Eliane Carvalho de Vasconcelos ◽

Débora Toledo Ramos ◽

...

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

High Performance ◽

Hplc Analysis ◽

Statistical Difference ◽

Deionized Water ◽

Dental Composites ◽

Maximal Concentration ◽

The Mean ◽

Mean Concentration

The aim of this study was to detect and quantify the main residual monomers released from composites, using high performance liquid chromatography (HPLC). Discs were made with dental composites (Herculite XRV, Tetric Ceram and Filtek Z250) and immersed in deionized water at 37ºC for 28 days, with water changes in 1, 7, 14 and 21 days. The mean concentration of residual monomers were subject to the Kruskal-Wallis test (p<0.05). Tetric Ceram exhibited significantly higher concentrations of leached monomers. Bis-GMA was the monomer released in lower concentrations for all the materials. There was no statistical difference between the amounts of TEGDMA and UDMA. Most of the monomers demonstrated maximal concentration at the 7-day period. The HPLC analysis identified Bis-GMA, TEGDMA and UDMA in detectable quantities for all the tested composites.

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Release of Formaldehyde from Dental Composites

Journal of Dental Research ◽

10.1177/00220345880670100901 ◽

1988 ◽

Vol 67 (10) ◽

pp. 1289-1294 ◽

Cited By ~ 93

Author(s):

H. Øysæd ◽

I.E. Ruyter ◽

I.J. Sjøvik Kleven

Keyword(s):

High Performance Liquid Chromatography ◽

Liquid Chromatography ◽

Composite Materials ◽

High Performance ◽

Degradation Products ◽

Polymeric Composite ◽

Dental Composites ◽

Inhibition Layer ◽

Continuous Release ◽

Hydrazone Derivative

Polymeric composite materials may contain releasable degradation products or unreacted constituents. Release of formaldehyde from nine different composites was investigated by means of HCHO-hydrazone derivative analyzed with high-performance liquid chromatography. Formation of formaldehyde was found in all the investigated materials. The highest concentrations were observed in specimens polymerized in contact with air. A correlation coefficient, r = 0.83, was found between released formaldehyde and the thickness of the unpolymerized surface inhibition layer. The formaldehyde concentrations were reduced when the inhibition layer was removed prior to testing. A continuous release of formaldehyde was evident during the first ten days. The release decreased with time, but was still detectable after 115 days.

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