scholarly journals Repeated Recovery ofStaphylococcus saprophyticusFrom the Urogenital Tracts of Women: Persistence Vs. Recurrence

1995 ◽  
Vol 2 (5) ◽  
pp. 218-222 ◽  
Author(s):  
M. E. Rupp ◽  
J. Han ◽  
R. V. Goering
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Restriction Fragment Length Polymorphism ◽  
Tract Infection ◽  
Gel Electrophoresis ◽  
Fragment Length Polymorphism ◽  
Chromosomal Dna ◽  
Dna Restriction ◽  
Typing Methods ◽  
Restriction Fragment Length

Objective:The purpose of this study was to determine whether colonization was persistent or recurrent in a small group of women who had repeated recovery ofStaphylococcus saprophyticusfrom their urogenital tracts.Methods:Paired isolates ofS. saprophyticusfrom each of the study subjects were genotypically typed by plasmid fingerprinting and comparison of chromosomal-DNA restriction fragment-length polymorphism patterns by field-inversion gel electrophoresis (FIGE) and contour-clamped homogenous electric-field (CHEF) electrophoresis.Results:All isolates ofS. saprophyticusfrom the study subjects were classified as genetically unique by each of the typing methods.Conclusions:The subjects experienced recurrent colonization with different isolates ofS. saprophyticus.These findings may have broader implications regarding the pathogenesis and recurrence ofS. saprophyticusurinary-tract infection.

Detection of tox A gene in Pseudomonas aeruginosa that isolates from different clinical cases by using PCR.

2018 ◽  
pp. 26
Author(s):  
Rana M. Abdullah Al-Shwaikh ◽  
Abbas Falih Alornaaouti
Keyword(s):  
Molecular Weight ◽  
Pseudomonas Aeruginosa ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Otitis Media ◽  
Urinary Tract Infections ◽  
Gel Electrophoresis ◽  
Wound Infections ◽  
Tract Infections

       Current study obtained (75) isolate of Pseudomonas aeruginosa collected from different cases included : 28 isolates from otitis media, 23 isolates from burn infections, 10 isolates from wound infections, 8 isolates from urinary tract infections and 6 isolates from blood, during the period between 1/9/2014 to 1/11/2014        The result revealed that the tox A gene was present in 54 isolates (72%) of Pseudomonas aeruginosa. The gel electrophoresis showed that the molecular weight of tox A gene was 352 bp. The result shows 17 isolates (60.71%) from otitis media has tox A gene, 18 isolates (78.26%) from burn followed by 8 isolate (80%) from wound infection and 5 isolates (62.5%) from urinary tract infection , finally 6 isolates (100%) from blood have this gene.

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