scholarly journals An automated determination of beta-glucuronidase activity in human serum with the Abbot VP bichromatic analyzer

1985 ◽  
Vol 7 (3) ◽  
pp. 152-154
Author(s):  
Constantino G. Bao ◽  
Francisco V. Alvarez
Keyword(s):  
Human Serum ◽  
Glucuronidase Activity ◽  
Automated Determination

Automated Determination of Acid Phosphatase

1966 ◽  
Vol 12 (4) ◽  
pp. 226-233 ◽  
Author(s):  
Bernard Klein ◽  
Morris Oklander ◽  
Stanley Morgenstern
Keyword(s):  
Acid Phosphatase ◽  
Human Serum ◽  
Phosphatase Activity ◽  
Acid Phosphatase Activity ◽  
Prostatic Acid Phosphatase ◽  
Biological Materials ◽  
Automated System ◽  
Automated Determination ◽  
Serum Acid Phosphatase

Abstract A procedure is presented for the automated determination of acid phosphatase activity in biological materials using the Robot Chemist. Although either phenylphosphate and α-naphthylphosphate may be used as substrate in this analysis, the procedure is described in detail for serum acid phosphatase using α-naphthylphosphate in 0.1 M citrate, pH 5.2, since this substrate is more selective for prostatic acid phosphatase in human serum. Enzymically generated aα- naphthol is determined by the Emerson reaction (alkaline aminoantipyrine and ferricyanide), modified for use with this automated system. Correlations are presented between the results obtained on the Robot Chemist and the identical procedure developed for the AutoAnalyzer.

scholarly journals Micro-bead injection spectroscopy for label-free automated determination of immunoglobulin G in human serum

2017 ◽  
Vol 410 (3) ◽  
pp. 981-988 ◽  
Author(s):  
Inês I. Ramos ◽  
Luís M. Magalhães ◽  
Luisa Barreiros ◽  
Salette Reis ◽  
José L. F. C. Lima ◽  
...  
Keyword(s):  
Human Serum ◽  
Immunoglobulin G ◽  
Label Free ◽  
Bead Injection ◽  
Automated Determination

Automated Determination of Acid Phosphatase

1966 ◽  
Vol 12 (5) ◽  
pp. 289-298 ◽  
Author(s):  
Bernard Klein ◽  
Joseph Auerbach
Keyword(s):  
Acid Phosphatase ◽  
Human Serum ◽  
Prostatic Acid Phosphatase ◽  
Inhibition Studies ◽  
Automated Determination

Abstract α-Naphthylphosphate is a selective substrate for the differentiation of prostatic acid phosphatase from erythrocytic acid phosphatase in human serum; its comparative specificity for prostatic enzyme is demonstrated by inhibition studies and by assay of mixtures of both enzymes in serum.

An Automated Procedure for the Determination of Ammonia in Tobacco

1972 ◽  
Vol 6 (4) ◽  
Author(s):  
P.F. Collins ◽  
W.W. Lawrence ◽  
J.F. Williams
Keyword(s):  
Standard Deviation ◽  
Relative Standard Deviation ◽  
Distillation Method ◽  
Relative Standard ◽  
Tobacco Sample ◽  
Automated Determination

AbstractA procedure for the automated determination of ammonia in tobacco has been developed. Ammonia is extracted from the ground tobacco sample with water and is determined with a Technicon Auto Analyser system which employs separation of the ammonia through volatilization followed by colourimetry using the phenate-hypochlorite reaction. The procedure has been applied to a variety of tobaccos containing from 0.02 to 0.5 % ammonia with an overall relative standard deviation of 2 %. The accuracy of the procedure as judged by recovery tests and by comparison to a manual distillation method is considered adequate

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