Abstract 5376: Systematic analysis of peptide linker length and liposomal polyethylene glycol coating on cellular uptake of peptide-targeted liposomal nanoparticles

Nanosized calcium phosphate was prepared by a reverse microemulsion method, with a 20~40 nm diameter, which is pH-responsive, nontoxic and colloidally stable in physiological solution. Polyethylene glycol modified calcium phosphate nanoparticles shifted the zeta potential to a neutral charge, which prolonged the nanoparticle circulation time and increased cellular uptake efficacy to targeted cells. The PEG-functionalized nanoparticles exhibit a great potential for efficaciously delivering hydrophobic anticancer drug, such as paclitaxel, to cells and tumors.

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Effect of hydrophobic scaffold on the cellular uptake and gene transfection activities of DNA-encapsulating liposomal nanoparticles via intracerebroventricular administration

International Journal of Pharmaceutics ◽

10.1016/j.ijpharm.2015.05.043 ◽

2015 ◽

Vol 490 (1-2) ◽

pp. 142-145 ◽

Cited By ~ 16

Author(s):

Hidetaka Akita ◽

Taichi Nakatani ◽

Kimiko Kuroki ◽

Katsumi Maenaka ◽

Kota Tange ◽

...

Keyword(s):

Cellular Uptake ◽

Gene Transfection ◽

Intracerebroventricular Administration ◽

Liposomal Nanoparticles

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Co-encapsulation of gemcitabine and tocotrienols in nanovesicles enhanced efficacy in pancreatic cancer

Nanomedicine ◽

10.2217/nnm-2020-0374 ◽

2021 ◽

Vol 16 (5) ◽

pp. 373-389

Author(s):

Geetha Maniam ◽

Chun-Wai Mai ◽

Mohd Zulkefeli ◽

Ju-Yen Fu

Keyword(s):

Pancreatic Cancer ◽

Polyethylene Glycol ◽

Cancer Cells ◽

Cellular Uptake ◽

Proof Of Concept ◽

Pancreatic Cancer Cells ◽

Polyethylene Glycol 1000 ◽

Span 60 ◽

Dual Drug

Aim: To synthesize niosomes co-encapsulating gemcitabine (GEM) and tocotrienols, and physicochemically characterize and evaluate the antipancreatic effects of the nanoformulation on Panc 10.05, SW 1990, AsPC-1 and BxPC-3 cells. Materials & methods: Niosomes-entrapping GEM and tocotrienols composed of Span 60, cholesterol and D-α-tocopheryl polyethylene glycol 1000 succinate were produced by Handjani-Vila and film hydration methods. Results: The film hydration produced vesicles measuring 161.9 ± 0.5 nm, approximately 50% smaller in size than Handjani-Vila method, with maximum entrapment efficiencies of 20.07 ± 0.22% for GEM and 34.52 ± 0.10% for tocotrienols. In Panc 10.05 cells, GEM’s antiproliferative effect was enhanced 2.78-fold in combination with tocotrienols. Niosomes produced a significant ninefold enhancement in cytotoxicity of the combination, supported by significantly higher cellular uptake of GEM in the cells. Conclusion: This study is a proof of concept on the synthesis of dual-drug niosomes and their efficacy on pancreatic cancer cells in vitro.

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- The Drug Encapsulation Efficiency, in vitro Drug Release, Cellular Uptake and Cytotoxicity of Paclitaxel-Loaded Poly(Lactide)–Tocopheryl Polyethylene Glycol Succinate Nanoparticles

Chemotherapeutic Engineering ◽

10.1201/b15645-15 ◽

2014 ◽

pp. 236-255

Keyword(s):

Polyethylene Glycol ◽

Drug Release ◽

Cellular Uptake ◽

Encapsulation Efficiency ◽

Glycol Succinate ◽

Drug Encapsulation ◽

In Vitro Drug Release ◽

Drug Encapsulation Efficiency

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Enhanced Cellular Uptake of Peptide-Targeted Nanoparticles through Increased Peptide Hydrophilicity and Optimized Ethylene Glycol Peptide-Linker Length

ACS Nano ◽

10.1021/nn4033954 ◽

2013 ◽

Vol 7 (9) ◽

pp. 8115-8127 ◽

Cited By ~ 53

Author(s):

Jared F. Stefanick ◽

Jonathan D. Ashley ◽

Basar Bilgicer

Keyword(s):

Ethylene Glycol ◽

Cellular Uptake ◽

Targeted Nanoparticles ◽

Linker Length

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Enhanced toxicity and cellular uptake of methotrexate-conjugated nanoparticles in folate receptor-positive cancer cells by decorating with folic acid-conjugated d -α-tocopheryl polyethylene glycol 1000 succinate

Colloids and Surfaces B Biointerfaces ◽

10.1016/j.colsurfb.2015.09.013 ◽

2015 ◽

Vol 136 ◽

pp. 383-393 ◽

Cited By ~ 8

Author(s):

Varaporn Buraphacheep Junyaprasert ◽

Sirithip Dhanahiranpruk ◽

Jiraphong Suksiriworapong ◽

Kittisak Sripha ◽

Primchanien Moongkarndi

Keyword(s):

Folic Acid ◽

Polyethylene Glycol ◽

Cancer Cells ◽

Cellular Uptake ◽

Folate Receptor ◽

Polyethylene Glycol 1000

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Effects of intravenous and subcutaneous administration on the pharmacokinetics, biodistribution, cellular uptake and immunostimulatory activity of CpG ODN encapsulated in liposomal nanoparticles

International Immunopharmacology ◽

10.1016/j.intimp.2007.04.002 ◽

2007 ◽

Vol 7 (8) ◽

pp. 1064-1075 ◽

Cited By ~ 51

Author(s):

Kaley D. Wilson ◽

Sameersingh G. Raney ◽

Laura Sekirov ◽

Ghania Chikh ◽

Susan D. deJong ◽

...

Keyword(s):

Cellular Uptake ◽

Subcutaneous Administration ◽

Cpg Odn ◽

Immunostimulatory Activity ◽

Liposomal Nanoparticles

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Impact of dynamic sub-populations within grafted chains on the protein binding and colloidal stability of PEGylated nanoparticles.

Nanoscale ◽

10.1039/d0nr08294e ◽

2021 ◽

Author(s):

Delyan Hristov ◽

Hender Lopez ◽

Yannick Ortin ◽

Kate O'Sullivan ◽

Kenneth A Dawson ◽

...

Keyword(s):

Polyethylene Glycol ◽

Protein Binding ◽

Cellular Uptake ◽

Blood Circulation ◽

Colloidal Stability ◽

Protein Recognition ◽

Biological Interaction ◽

Pegylated Nanoparticles

Polyethylene glycol grafting has played a central role in preparing the surfaces of nano-probes for biological interaction; to extend blood circulation times and to modulate protein recognition and cellular uptake....

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