Abstract 2194: Synergistic cytotoxicity of targeted liposomes containing doxorubicin and C6-ceramide against nucleolin-overexpressing ovarian cancer cells is supported by the downregulation of the Akt pathway

2017 ◽  
Author(s):  
Ana Filipa Cruz ◽  
Nuno André Fonseca ◽  
Susana F. Sampaio ◽  
Vera Moura ◽  
Ramiro D. Almeida ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Akt Pathway ◽  
Ovarian Cancer Cells ◽  
Synergistic Cytotoxicity ◽  
C6 Ceramide

scholarly journals The Enhanced Efficacy of Intracellular Delivery of Doxorubicin/C6-Ceramide Combination Mediated by the F3 Peptide/Nucleolin System Is Supported by the Downregulation of the PI3K/Akt Pathway

Cancers ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 3052
Author(s):  
Ana F. Cruz ◽  
Mariana B. Caleiras ◽  
Nuno A. Fonseca ◽  
Nélio Gonçalves ◽  
Vera M. Mendes ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Intracellular Delivery ◽  
Akt Pathway ◽  
Ovarian Cancer Cells ◽  
Intrinsic Resistance ◽  
Ovarian Cancer Stem Cells ◽  
Ovarian Cancer Cell Lines ◽  
C6 Ceramide

Targeting multiple cellular populations is of high therapeutic relevance for the tackling of solid tumors heterogeneity. Herein, the ability of pegylated and pH-sensitive liposomes, functionalized with the nucleolin-binding F3 peptide and containing doxorubicin (DXR)/C6-ceramide synergistic combination, to target, in vitro, ovarian cancer, including ovarian cancer stem cells (CSC), was assessed. The underlying molecular mechanism of action of the nucleolin-mediated intracellular delivery of C6-ceramide to cancer cells was also explored. The assessment of overexpression of surface nucleolin expression by flow cytometry was critical to dissipate differences identified by Western blot in membrane/cytoplasm of SKOV-3, OVCAR-3 and TOV-112D ovarian cancer cell lines. The former was in line with the significant extent of uptake into (bulk) ovarian cancer cells, relative to non-targeted and non-specific counterparts. This pattern of uptake was recapitulated with putative CSC-enriched ovarian SKOV-3 and OVCAR-3 sub-population (EpCAMhigh/CD44high). Co-encapsulation of DXR:C6-ceramide into F3 peptide-targeted liposomes improved cytotoxic activity relative to liposomes containing DXR alone, in an extent that depended on the intrinsic resistance to DXR and on the incubation time. The enhanced cytotoxicity of the targeted combination was mechanistically supported by the downregulation of PI3K/Akt pathway by C6-ceramide, only among the nucleolin-overexpressing cancer cells presenting a basal p-Akt/total Akt ratio lower than 1.

MiR-181c sensitizes ovarian cancer cells to paclitaxel by targeting GRP78 through the PI3K/Akt pathway

2021 ◽  
Author(s):  
Li-ying Zhang ◽  
Jia-ying Yu ◽  
Yan-long Leng ◽  
Ran-ran Zhu ◽  
Hong-xian Liu ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Akt Pathway ◽  
Ovarian Cancer Cells

Abstract 3083A: C6-ceramide nanoliposomes sensitize paclitaxel resistant ovarian cancer cells to chemotherapy treatment

2017 ◽  
Author(s):  
Danielle C. Llaneza ◽  
Tye G. Deering ◽  
Samantha G. Sherwood ◽  
John R. Cornelison ◽  
Mark Kester ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Ovarian Cancer Cells ◽  
Chemotherapy Treatment ◽  
C6 Ceramide

HPIP promotes epithelial-mesenchymal transition and cisplatin resistance in ovarian cancer cells through PI3K/AKT pathway activation

2016 ◽  
Vol 40 (2) ◽  
pp. 133-144 ◽  
Author(s):  
Suresh Bugide ◽  
Vijay Kumar Gonugunta ◽  
Vasudevarao Penugurti ◽  
Vijaya Lakshmi Malisetty ◽  
Ratna K. Vadlamudi ◽  
...  
Keyword(s):  
Ovarian Cancer ◽  
Cancer Cells ◽  
Cisplatin Resistance ◽  
Epithelial Mesenchymal Transition ◽  
Akt Pathway ◽  
Ovarian Cancer Cells ◽  
Mesenchymal Transition ◽  
Pathway Activation

Fluvastatin and cisplatin demonstrate synergistic cytotoxicity in epithelial ovarian cancer cells

2010 ◽  
Vol 119 (3) ◽  
pp. 549-556 ◽  
Author(s):  
Barbie Taylor-Harding ◽  
Sandra Orsulic ◽  
Beth Y. Karlan ◽  
Andrew J. Li
Keyword(s):  
Ovarian Cancer ◽  
Epithelial Ovarian Cancer ◽  
Cancer Cells ◽  
Ovarian Cancer Cells ◽  
Synergistic Cytotoxicity
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