Abstract 4126: Validation of hypoglycosylated MUC1-CIN85 protein-protein interaction as a new therapeutic target for prevention of cancer invasion and metastasis

2017 ◽  
Author(s):  
Sandra Cascio ◽  
Jacque Faylo ◽  
Raahul Sriram ◽  
Anda Vlad ◽  
Carlos Camacho ◽  
...  
Keyword(s):  
Protein Interaction ◽  
Therapeutic Target ◽  
Cancer Invasion ◽  
Invasion And Metastasis ◽  
Protein Protein Interaction ◽  
Prevention Of Cancer

scholarly journals Validation of the Hsp70–Bag3 Protein–Protein Interaction as a Potential Therapeutic Target in Cancer

2015 ◽  
Vol 14 (3) ◽  
pp. 642-648 ◽  
Author(s):  
Xiaokai Li ◽  
Teresa Colvin ◽  
Jennifer N. Rauch ◽  
Diego Acosta-Alvear ◽  
Martin Kampmann ◽  
...  
Keyword(s):  
Protein Interaction ◽  
Therapeutic Target ◽  
Potential Therapeutic Target ◽  
Protein Protein Interaction

scholarly journals Circular RNA—Is the Circle Perfect?

Biomolecules ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1755
Author(s):  
Lavinia Caba ◽  
Laura Florea ◽  
Cristina Gug ◽  
Daniela Cristina Dimitriu ◽  
Eusebiu Vlad Gorduza
Keyword(s):  
Cell Cycle ◽  
Protein Interaction ◽  
Therapeutic Target ◽  
Circular Rna ◽  
Regulatory Role ◽  
Regulation Of Transcription ◽  
Distinct Class ◽  
Protein Protein Interaction ◽  
Non Coding Rna ◽  
Microrna Sponge

Circular RNA (circRNA) is a distinct class of non-coding RNA produced, in principle, using a back-splicing mechanism, conserved during evolution, with increased stability and a tissue-dependent expression. Circular RNA represents a functional molecule with roles in the regulation of transcription and splicing, microRNA sponge, and the modulation of protein–protein interaction. CircRNAs are involved in essential processes of life such as apoptosis, cell cycle, and proliferation. Due to the regulatory role (upregulation/downregulation) in pathogenic mechanisms of some diseases (including cancer), its potential roles as a biomarker or therapeutic target in these diseases were studied. This review focuses on the importance of circular RNA in cancer.

scholarly journals Protein–protein interaction analysis reveals a novel cancer stem cell related target TMEM17 in colorectal cancer

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Zhao-liang Yu ◽  
Yu-feng Chen ◽  
Bin Zheng ◽  
Ze-rong Cai ◽  
Yi-feng Zou ◽  
...  
Keyword(s):  
Colorectal Cancer ◽  
Stem Cell ◽  
Protein Interaction ◽  
Therapeutic Target ◽  
Enrichment Analysis ◽  
Gene Set Enrichment Analysis ◽  
Functional Study ◽  
Clinical Samples ◽  
Stem Cell Markers ◽  
Protein Protein Interaction

Abstract Background Cancer stem cells (CSCs) are a small subpopulation of cells within tumors with stem cell property. Increased evidence suggest that CSCs could be responsible for chemoresistance and recurrence in colorectal cancer (CRC). However, a reliable therapeutic target on CSCs is still lacking. Methods Here we describe a two-step strategy to generate CSC targets with high selectivity for colon stem cell markers, specific proteins that are interacted with CSC markers were selected and subsequently validated in a survival analysis. TMEM17 protein was found and its biological functions in CRC cells were further examined. Finally, we utilized the Gene Set Enrichment Analysis (GSEA) to investigate the potential mechanisms of TMEM17 in CRC. Results By combining protein–protein interaction (PPI) database and high-throughput gene profiles, network analysis revealed a cluster of colon CSCs related genes. In the cluster, TMEM17 was identified as a novel CSCs related gene. The results of in-vitro functional study demonstrated that TMEM17 depletion can suppress the proliferation of CRC cells and sensitize CRC cells to chemotherapy drugs. Enrichment analysis revealed that the expression of TMEM17 is associated with the magnitude of activation of the Wnt/β-catenin pathway. Further validation in clinical samples demonstrated that the TMEM17 expression was much higher in tumor than normal tissue and was associated with poor survival in CRC patients. Conclusion Collectively, our finding unveils the critical role of TMEM17 in CRC and TMEM17 could be a potential effective therapeutic target for tumor recurrence and chemoresistance in the colorectal cancer (CRC).

An efficient transient assays system using Agrobacterium-mediated transformation of onion (Allium cepa) epidermal cells

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Yu-Miao Zhang ◽  
Jun Wang ◽  
Tao Wu
Keyword(s):  
Subcellular Localization ◽  
Protein Interaction ◽  
Protein Interactions ◽  
Epidermal Cells ◽  
Cyclin Dependent Kinase ◽  
Protein Subcellular Localization ◽  
Protein Protein Interactions ◽  
Efficient System ◽  
Protein Protein Interaction ◽  
Onion Epidermal Cells

In this study, the Agrobacterium infection medium, infection duration, detergent, and cell density were optimized. The sorghum-based infection medium (SbIM), 10-20 min infection time, addition of 0.01% Silwet L-77, and Agrobacterium optical density at 600 nm (OD600), improved the competence of onion epidermal cells to support Agrobacterium infection at >90% efficiency. Cyclin-dependent kinase D-2 (CDKD-2) and cytochrome c-type biogenesis protein (CYCH), protein-protein interactions were localized. The optimized procedure is a quick and efficient system for examining protein subcellular localization and protein-protein interaction.

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