scholarly journals Abstract 5780: 3Dex-vivoassay platform using primary lung cancer cells in malignant pleural effusions as predictor for clinical outcome of personalized chemotherapy

2017 ◽  
Author(s):  
Cheng-guang Wu ◽  
Francesca Chiovaro ◽  
Tamara Tanos ◽  
Alex Soltermann ◽  
Sumeer Dhar
Keyword(s):  
Lung Cancer ◽  
Clinical Outcome ◽  
Cancer Cells ◽  
Primary Lung Cancer ◽  
Lung Cancer Cells ◽  
Pleural Effusions ◽  
Malignant Pleural Effusions ◽  
Personalized Chemotherapy

scholarly journals Tubulin acetylation enhances lung cancer resistance to paclitaxel-induced cell death through Mcl-1 stabilization

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Onsurang Wattanathamsan ◽  
Rawikorn Thararattanobon ◽  
Ratchanee Rodsiri ◽  
Pithi Chanvorachote ◽  
Chanida Vinayanuwattikun ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Death ◽  
Cancer Cells ◽  
Trichostatin A ◽  
Primary Lung Cancer ◽  
Lung Cancer Cells ◽  
Cancer Resistance ◽  
Apoptosis Resistance ◽  
Tubulin Acetylation ◽  
Cancer Aggressiveness

AbstractThe posttranslational modifications (PTMs) of microtubules have been reported to play an important role in cancer aggressiveness, including apoptosis resistance. In this study, we aimed to investigate the biological role of microtubule PTMs in the regulation of paclitaxel responsiveness. The acetylated tubulin (Ace-tub) level was strongly associated with paclitaxel sensitivity, as observed in patient-derived primary lung cancer cells and xenografted immunodeficient mice. We showed that paclitaxel-resistant H460 lung cancer cells, generated by a stepwise increase in paclitaxel, exhibited markedly increased tubulin acetylation and consequently acquired paclitaxel resistance. Upregulation of tubulin acetylation by overexpression of α-tubulin acetyltransferase 1 wild-type (αTAT1wt), an enzyme required for acetylation, or by treatment with trichostatin A (TSA), a histone deacetylase 6 (HDAC6) inhibitor, significantly attenuated paclitaxel-induced apoptosis. Investigation of the underlying mechanism revealed that the levels of antiapoptotic Mcl-1 appeared to increase in αTAT1wt-overexpressing and TSA-treated cells compared to control cells, whereas the levels of other antiapoptotic regulatory proteins were unchanged. On the other hand, decreased tubulin acetylation by αTAT1 RNA interference downregulated Mcl-1 expression in patient-derived primary lung cancer and paclitaxel-resistant lung cancer cells. A microtubule sedimentation assay demonstrated that Mcl-1 binds to microtubules preferentially at Ace-type, which prolongs the Mcl-1 half-life (T1/2). Furthermore, immunoprecipitation analysis revealed that polyubiquitination of Mcl-1 was extensively decreased in response to TSA treatment. These data indicate that tubulin acetylation enhances the resistance to paclitaxel-induced cell death by stabilizing Mcl-1 and protecting it from ubiquitin–proteasome-mediated degradation.

Characterisation of the Phosphatidylinositol 3-Kinase Pathway in Non-Small Cell Lung Cancer Cells Isolated from Pleural Effusions

Oncology ◽  
2016 ◽  
Vol 90 (5) ◽  
pp. 280-288 ◽  
Author(s):  
Martina Puglisi ◽  
Adam Stewart ◽  
Parames Thavasu ◽  
Michael Frow ◽  
Suzanne Carreira ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Small Cell Lung Cancer ◽  
Cancer Cells ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Lung Cancer Cells ◽  
Pleural Effusions ◽  
Phosphatidylinositol 3 Kinase ◽  
Small Cell Lung ◽  
Kinase Pathway

scholarly journals RANK rewires energy homeostasis in lung cancer cells and drives primary lung cancer

2017 ◽  
Vol 31 (20) ◽  
pp. 2099-2112 ◽  
Author(s):  
Shuan Rao ◽  
Verena Sigl ◽  
Reiner Alois Wimmer ◽  
Maria Novatchkova ◽  
Alexander Jais ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Energy Homeostasis ◽  
Primary Lung Cancer ◽  
Lung Cancer Cells

scholarly journals Analysis of nuclear DNA content in primary lung cancer cells by means of flow cytometry.

Haigan ◽  
1987 ◽  
Vol 27 (2) ◽  
pp. 125-132 ◽  
Author(s):  
Hiroshi Isobe ◽  
Masami Ito ◽  
Toru Shimizu ◽  
Hirotoshi Dosaka ◽  
Yoshikazu Araya ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Flow Cytometry ◽  
Cancer Cells ◽  
Dna Content ◽  
Nuclear Dna ◽  
Primary Lung Cancer ◽  
Nuclear Dna Content ◽  
Lung Cancer Cells

Effective method for the isolation and proliferation of primary lung cancer cells from patient lung tissues

2013 ◽  
Vol 35 (8) ◽  
pp. 1165-1174 ◽  
Author(s):  
Joseph Seo ◽  
Soon-Jung Park ◽  
Jhingook Kim ◽  
So-Jung Choi ◽  
Sung-Hwan Moon ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Primary Lung Cancer ◽  
Lung Cancer Cells

PARP Inhibitor Activates the Intrinsic Pathway of Apoptosis in Primary Lung Cancer Cells

2014 ◽  
Vol 32 (7) ◽  
pp. 339-348 ◽  
Author(s):  
Nupur N. Gangopadhyay ◽  
James D. Luketich ◽  
Amy Opest ◽  
Rodney Landreneau ◽  
Matthew J. Schuchert
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Parp Inhibitor ◽  
Primary Lung Cancer ◽  
Lung Cancer Cells ◽  
Intrinsic Pathway
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