Effective method for the isolation and proliferation of primary lung cancer cells from patient lung tissues

2013 ◽  
Vol 35 (8) ◽  
pp. 1165-1174 ◽  
Author(s):  
Joseph Seo ◽  
Soon-Jung Park ◽  
Jhingook Kim ◽  
So-Jung Choi ◽  
Sung-Hwan Moon ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Primary Lung Cancer ◽  
Lung Cancer Cells

scholarly journals Tubulin acetylation enhances lung cancer resistance to paclitaxel-induced cell death through Mcl-1 stabilization

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Onsurang Wattanathamsan ◽  
Rawikorn Thararattanobon ◽  
Ratchanee Rodsiri ◽  
Pithi Chanvorachote ◽  
Chanida Vinayanuwattikun ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cell Death ◽  
Cancer Cells ◽  
Trichostatin A ◽  
Primary Lung Cancer ◽  
Lung Cancer Cells ◽  
Cancer Resistance ◽  
Apoptosis Resistance ◽  
Tubulin Acetylation ◽  
Cancer Aggressiveness

AbstractThe posttranslational modifications (PTMs) of microtubules have been reported to play an important role in cancer aggressiveness, including apoptosis resistance. In this study, we aimed to investigate the biological role of microtubule PTMs in the regulation of paclitaxel responsiveness. The acetylated tubulin (Ace-tub) level was strongly associated with paclitaxel sensitivity, as observed in patient-derived primary lung cancer cells and xenografted immunodeficient mice. We showed that paclitaxel-resistant H460 lung cancer cells, generated by a stepwise increase in paclitaxel, exhibited markedly increased tubulin acetylation and consequently acquired paclitaxel resistance. Upregulation of tubulin acetylation by overexpression of α-tubulin acetyltransferase 1 wild-type (αTAT1wt), an enzyme required for acetylation, or by treatment with trichostatin A (TSA), a histone deacetylase 6 (HDAC6) inhibitor, significantly attenuated paclitaxel-induced apoptosis. Investigation of the underlying mechanism revealed that the levels of antiapoptotic Mcl-1 appeared to increase in αTAT1wt-overexpressing and TSA-treated cells compared to control cells, whereas the levels of other antiapoptotic regulatory proteins were unchanged. On the other hand, decreased tubulin acetylation by αTAT1 RNA interference downregulated Mcl-1 expression in patient-derived primary lung cancer and paclitaxel-resistant lung cancer cells. A microtubule sedimentation assay demonstrated that Mcl-1 binds to microtubules preferentially at Ace-type, which prolongs the Mcl-1 half-life (T1/2). Furthermore, immunoprecipitation analysis revealed that polyubiquitination of Mcl-1 was extensively decreased in response to TSA treatment. These data indicate that tubulin acetylation enhances the resistance to paclitaxel-induced cell death by stabilizing Mcl-1 and protecting it from ubiquitin–proteasome-mediated degradation.

scholarly journals RANK rewires energy homeostasis in lung cancer cells and drives primary lung cancer

2017 ◽  
Vol 31 (20) ◽  
pp. 2099-2112 ◽  
Author(s):  
Shuan Rao ◽  
Verena Sigl ◽  
Reiner Alois Wimmer ◽  
Maria Novatchkova ◽  
Alexander Jais ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Energy Homeostasis ◽  
Primary Lung Cancer ◽  
Lung Cancer Cells

scholarly journals Analysis of nuclear DNA content in primary lung cancer cells by means of flow cytometry.

Haigan ◽  
1987 ◽  
Vol 27 (2) ◽  
pp. 125-132 ◽  
Author(s):  
Hiroshi Isobe ◽  
Masami Ito ◽  
Toru Shimizu ◽  
Hirotoshi Dosaka ◽  
Yoshikazu Araya ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Flow Cytometry ◽  
Cancer Cells ◽  
Dna Content ◽  
Nuclear Dna ◽  
Primary Lung Cancer ◽  
Nuclear Dna Content ◽  
Lung Cancer Cells

PARP Inhibitor Activates the Intrinsic Pathway of Apoptosis in Primary Lung Cancer Cells

2014 ◽  
Vol 32 (7) ◽  
pp. 339-348 ◽  
Author(s):  
Nupur N. Gangopadhyay ◽  
James D. Luketich ◽  
Amy Opest ◽  
Rodney Landreneau ◽  
Matthew J. Schuchert
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Parp Inhibitor ◽  
Primary Lung Cancer ◽  
Lung Cancer Cells ◽  
Intrinsic Pathway

scholarly journals Spheroid Culture of Primary Lung Cancer Cells with Neuregulin 1/HER3 Pathway Activation

2013 ◽  
Vol 8 (2) ◽  
pp. 131-139 ◽  
Author(s):  
Hiroko Endo ◽  
Jiro Okami ◽  
Hiroaki Okuyama ◽  
Toru Kumagai ◽  
Junji Uchida ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Primary Lung Cancer ◽  
Lung Cancer Cells ◽  
Spheroid Culture ◽  
Neuregulin 1 ◽  
Pathway Activation

scholarly journals Development of DNA Aptamers to Native EpCAM for Isolation of Lung Circulating Tumor Cells from Human Blood

Cancers ◽  
2019 ◽  
Vol 11 (3) ◽  
pp. 351 ◽  
Author(s):  
Galina Zamay ◽  
Olga Kolovskaya ◽  
Tatiana Ivanchenko ◽  
Tatiana Zamay ◽  
Dmitry Veprintsev ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
Primary Lung Cancer ◽  
Lung Cancer Cells ◽  
Clinical Samples ◽  
Dna Aptamers ◽  
Microscopy Imaging ◽  
Lung Cancer Patients

We selected DNA aptamers to the epithelial cell adhesion molecule (EpCAM) expressed on primary lung cancer cells isolated from the tumors of patients with non-small cell lung cancer using competitive displacement of aptamers from EpCAM by a corresponding antibody. The resulting aptamers clones showed good nanomolar affinity to EpCAM-positive lung cancer cells. Confocal microscopy imaging and spectral profiling of lung cancer tissues confirmed the same protein target for the aptamers and anti-EpCAM antibodies. Furthermore, the resulted aptamers were successfully applied for isolation and detection of circulating tumor cells in clinical samples of peripheral blood of lung cancer patients.

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