Abstract P1-06-06: Mammary stem cell modulation of wildtype and Trp53 null stem cells by CAPE (caffeic acid phenethyl ester), a potential therapeutic agent

2016 ◽  
Author(s):  
C Omene ◽  
M Patel ◽  
K Kannan ◽  
A Heguy ◽  
MH Barcellos-Hoff
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Caffeic Acid ◽  
Therapeutic Agent ◽  
Caffeic Acid Phenethyl Ester ◽  
Mammary Stem Cell ◽  
Potential Therapeutic Agent ◽  
Mammary Stem

scholarly journals 3D Differentiation Of Mammosphere Derived Macaca fascicularis’s Mammary Stem Cells

2019 ◽  
Vol 3 (1) ◽  
Author(s):  
Silmi Mariya
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Mammary Gland ◽  
Cell Population ◽  
Mammary Stem Cell ◽  
Stem Cell Population ◽  
Stem Cell Markers ◽  
Mammary Stem Cells ◽  
Surgical Biopsy ◽  
Mammary Stem

The mammary gland contains adult stem cells that are capable of self-renewal.  This population plays an important role in the development of mammary gland and breast cancer pathogenesis. The studies of mammary stem cells are limited due to the difficulty to acquire and expand adult stem cell population in an undifferentiated state. In this study, we developed mammosphere cultures of nulliparous cynomolgus monkeys (Macaca fascicularis; Mf) as a culture system to enrich mammary stem cells. This species has similarity of mammary gland structure as humans including anatomy, developmental stages, and lobule profile of mammary gland. The use of stem cells from primate animals is essential to bridge the knowledge gaps resulting from stem cell research using rodents for clinical trials in human. Small samples of mammary tissues were collected by surgical biopsy; cells were cultured as monolayer and cryopreserved. Cryopreserved cells were cultured into mammospheres, and the expression of markers for mammary stem cells was evaluated using qPCR. Cells were further differentiated with 3D approaches to evaluate morphology and organoid budding. The study showed that mammosphere culture resulted in an increase in the expression of mammary stem cell markers with each passage. The 3D differentiation in matrigel allowed for organoid formation. Mammary gland stem cells have been successfully differentiated which characterized by CSN2 marker expression and differentiation regulators marker STAT5 and GATA3. The results indicate that mammospheres can be successfully developed derived from breast tissue of nulliparous Mf collected via surgical biopsy. As the mammosphere allows for enrichment of mammary stem cell population, the findings also suggest that a 3-dimensional system is efficient as in-vitro model to study mammary stem cells and a useful system to study mammary differentiation in regards to cancer prevention.

scholarly journals Caffeic Acid Phenethyl Ester Is a Potential Therapeutic Agent for Oral Cancer

2015 ◽  
Vol 16 (12) ◽  
pp. 10748-10766 ◽  
Author(s):  
Ying-Yu Kuo ◽  
Wai-Tim Jim ◽  
Liang-Cheng Su ◽  
Chi-Jung Chung ◽  
Ching-Yu Lin ◽  
...  
Keyword(s):  
Oral Cancer ◽  
Caffeic Acid ◽  
Therapeutic Agent ◽  
Caffeic Acid Phenethyl Ester ◽  
Potential Therapeutic Agent

scholarly journals Caffeic Acid Phenethyl Ester with Mesenchymal Stem Cells Improve Behavioral and Histopathological Changes in the Rat Model of Parkinson' Disease

2021 ◽  
Author(s):  
Khojasteh Rahimi Jaberi ◽  
◽  
Manouchehr Safari ◽  
Vahid Semnani ◽  
Hamid Reza Sameni ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Caffeic Acid ◽  
Dopaminergic Neurons ◽  
Intranasal Administration ◽  
Caffeic Acid Phenethyl Ester ◽  
Substantia Nigra Pars Compacta ◽  
Tunel Staining ◽  
Apoptotic Cells ◽  
Protective Effects

Introduction: Parkinson's disease (PD) is the result of the destruction of Dopaminergic neurons in the brain. The aim of this study was to investigate the protective effects of natural antioxidants such as caffeic acid phenethyl ester for the maintenance of these neurons. Methods: Caffeic acid phenethyl ester (CAPE) is one of the main ingredients of Propolis. Intranasal administration of (1-methyl-4-phenyl-2;3;4;6-tetrahydropyridine) MPTP was used to generate PD model in rats. 2× 106 bone marrow stem cells (BMSCs) were injected from tail vein. Behavioral test, Immunohistochemistry, DiI, cresyl fast violet, TUNEL staining were evaluated, 2 weeks after treatment. Results: DiI staining method revealed in all treatment groups using stem cells, the cells migrated to the substantia nigra pars compacta after injection. Treatment with CAPE significantly protects dopaminergic neurons from MPTP. The highest number of tyrosine hydroxylase (TH (positive neurons was seen in group Pre CAPE+PD+Stem cell. The number of TH+ cells in all groups that received CAPE was significant in compared to groups that received the stem cells only (P <0.001). Intranasal administration of MPTP significantly increase the number of apoptotic cells. The lowest number of apoptotic cells was in group Pre CAPE+PD+ Stem cell. Conclusion: The results showed that the use of CAPE and stem cells in Parkinson's rats caused a significant reduction in the apoptotic cells.

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