Abstract 48: Characterization of Human Papillomavirus (HPV) Sequence and Integration and Analysis of Immune Response Genes for Application in Guatemalan Cervical Cancer

2021 ◽  
Author(s):  
Nicole Rossi ◽  
Michael Dean
Keyword(s):  
Cervical Cancer ◽  
Immune Response ◽  
Human Papillomavirus ◽  
Immune Response Genes

scholarly journals Human Papillomavirus Type 16 E7 Peptide-Directed CD8+ T Cells from Patients with Cervical Cancer Are Cross-Reactive with the Coronavirus NS2 Protein

2003 ◽  
Vol 77 (9) ◽  
pp. 5464-5474 ◽  
Author(s):  
Katja Nilges ◽  
Hanni Höhn ◽  
Henryk Pilch ◽  
Claudia Neukirch ◽  
Kirsten Freitag ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Immune Response ◽  
T Cells ◽  
Human Papillomavirus ◽  
T Cell ◽  
T Cell Responses ◽  
Cross Reactivity ◽  
Antiviral Immune Response ◽  
Human Papillomavirus Type 16 ◽  
Cell Responses

ABSTRACT Human papillomavirus type 16 (HPV16) E6 and E7 oncoproteins are required for cellular transformation and represent candidate targets for HPV-specific and major histocompatibility complex class I-restricted CD8+-T-cell responses in patients with cervical cancer. Recent evidence suggests that cross-reactivity represents the inherent nature of the T-cell repertoire. We identified HLA-A2 binding HPV16 E7 variant peptides from human, bacterial, or viral origin which are able to drive CD8+-T-cell responses directed against wild-type HPV16 E7 amino acid 11 to 19/20 (E711-19/20) epitope YMLDLQPET(T) in vitro. CD8+ T cells reacting to the HLA-A2-presented peptide from HPV16 E711-19(20) recognized also the HLA-A2 binding peptide TMLDIQPED (amino acids 52 to 60) from the human coronavirus OC43 NS2 gene product. Establishment of coronavirus NS2-specific, HLA-A2-restricted CD8+-T-cell clones and ex vivo analysis of HPV16 E7 specific T cells obtained by HLA-A2 tetramer-guided sorting from PBL or tumor-infiltrating lymphocytes obtained from patients with cervical cancer showed that cross-reactivity with HPV16 E711-19(20) and coronavirus NS252-60 represents a common feature of this antiviral immune response defined by cytokine production. Zero of 10 patients with carcinoma in situ neoplasia and 3 of 18 patients with cervical cancer showed ≥0.1% HPV16 E7-reactive T cells in CD8+ peripheral blood lymphocytes. In vivo priming with HPV16 was confirmed in patients with cervical cancer or preinvasive HPV16-positive lesions using HLA-A2 tetramer complexes loaded with the E6-derived epitope KLPQLCTEL. In contrast, we could not detect E6-reactive T cells in healthy individuals. These data imply that the measurement of the HPV16 E711-19(20) CD8+-T-cell response may reflect cross-reactivity with a common pathogen and that variant peptides may be employed to drive an effective cellular immune response against HPV.

Humoral Immune Response Against Human Papillomavirus as Source of Biomarkers for the Prediction and Detection of Cervical Cancer

2016 ◽  
Vol 29 (2) ◽  
pp. 83-94 ◽  
Author(s):  
Lourdes Gutierrez-Xicotencatl ◽  
Dolores Azucena Salazar-Piña ◽  
Adolfo Pedroza-Saavedra ◽  
Lilia Chihu-Amparan ◽  
Angelica Nallelhy Rodriguez-Ocampo ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Immune Response ◽  
Human Papillomavirus ◽  
Humoral Immune Response ◽  
Humoral Immune

Establishment and characterization of a human-papillomavirus negative, p53-mutation negative human cervical cancer cell line

1996 ◽  
Vol 102 (1-2) ◽  
pp. 173-181 ◽  
Author(s):  
Cheng-Yang Chou ◽  
Yen-Hui Chen ◽  
Ching-Cherng Tzeng ◽  
Yueh-Chin Cheng ◽  
Chin-Feng Chang ◽  
...  
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus ◽  
Cell Line ◽  
Cancer Cell ◽  
Cancer Cell Line ◽  
P53 Mutation ◽  
Cervical Cancer Cell Line ◽  
Human Cervical Cancer Cell ◽  
Human Cervical Cancer

scholarly journals Activities of E6 Protein of Human Papillomavirus 16 Asian Variant on miR-21 Up-regulation and Expression of Human Immune Response Genes

2015 ◽  
Vol 16 (9) ◽  
pp. 3961-3968 ◽  
Author(s):  
Peechanika Chopjitt ◽  
Chamsai Pientong ◽  
Sureewan Bumrungthai ◽  
Bunkerd Kongyingyoes ◽  
Tipaya Ekalaksananan
Keyword(s):  
Immune Response ◽  
Human Papillomavirus ◽  
Human Papillomavirus 16 ◽  
Immune Response Genes ◽  
Human Immune Response ◽  
Human Immune ◽  
E6 Protein

Expression and characterization of transforming protein E7 from cervical cancer-associated human papillomavirus type 31

2013 ◽  
Vol 18 (6) ◽  
pp. 1109-1115
Author(s):  
Seung Bum Choi ◽  
Young Soon Kang ◽  
Sun Kwon Bang ◽  
In Seok Bang
Keyword(s):  
Cervical Cancer ◽  
Human Papillomavirus

Pyrosequencing and characterization of immune response genes from the American dog tick, Dermacentor variabilis (L.)

2010 ◽  
Vol 19 (5) ◽  
pp. 617-630 ◽  
Author(s):  
D. C. Jaworski ◽  
Z. Zou ◽  
C. J. Bowen ◽  
N. B. Wasala ◽  
R. Madden ◽  
...  
Keyword(s):  
Immune Response ◽  
Dermacentor Variabilis ◽  
American Dog Tick ◽  
Immune Response Genes ◽  
Dog Tick
Sign in / Sign up

Export Citation Format

Share Document