Abstract A18: Geranylgeranoic acid (GGA) induced autophagic cell death through induction of unfolded protein response (UPR) and suppression of mTOR signaling pathway

2015 ◽  
Author(s):  
Chieko Iwao ◽  
Yoshihiro Shidoji
Keyword(s):  
Cell Death ◽  
Unfolded Protein Response ◽  
Signaling Pathway ◽  
Mtor Signaling ◽  
Autophagic Cell Death ◽  
Mtor Signaling Pathway ◽  
Unfolded Protein ◽  
Protein Response

scholarly journals Lipopolysaccharide Induces Autophagic Cell Death through the PERK-Dependent Branch of the Unfolded Protein Response in Human Alveolar Epithelial A549 Cells

2015 ◽  
Vol 36 (6) ◽  
pp. 2403-2417 ◽  
Author(s):  
Shaoying Li ◽  
Liang Guo ◽  
Pin Qian ◽  
Yunfeng Zhao ◽  
Ao Liu ◽  
...  
Keyword(s):  
Cell Death ◽  
Cell Viability ◽  
Er Stress ◽  
Cell Survival ◽  
Unfolded Protein Response ◽  
A549 Cells ◽  
Autophagic Cell Death ◽  
Alveolar Epithelial ◽  
Unfolded Protein ◽  
Protein Response

Background: Alveolar epithelial cell death plays a critical role in the pathogenesis of lipopolysaccharide (LPS)-induced acute lung injury. Increased autophagy has a dual effect on cell survival. However, it is not known whether autophagy promotes death or survival in human alveolar epithelial cells exposed to LPS. Methods: Genetic and pharmacological approaches were used to evaluate the effect of autophagy on A549 cell viability upon LPS exposure. The endoplasmic reticulum (ER) stress and unfolded protein response (UPR) pathways were examined with immunoblotting studies to further explore underlying mechanisms. Results: Treatment with LPS (50 µg/ml) led to autophagy activation and decreased cell viability in A549 cells. Blocking autophagy via short interfering RNA or inhibitor significantly decreased, whereas rapamycin increased, the LPS-induced effect on viability. ER stress was activated in LPS-stimulated A549 cells, and ER stress inhibitor reduced LPS-induced autophagy. LPS activated only the PERK pathway and had rarely effect on the ATF6 and IRE1 branches of the UPR in A549 cells. Moreover, the knockdown of PERK and ATF4 attenuated LPS-induced autophagy and promoted cell survival. Conclusion: In human alveolar epithelial A549 cells, LPS induces autophagic cell death that depends on the activation of the PERK branch of the UPR upon ER stress.

scholarly journals Fisetin induces autophagic cell death through suppression of mTOR signaling pathway in prostate cancer cells

2010 ◽  
Vol 31 (8) ◽  
pp. 1424-1433 ◽  
Author(s):  
Y. Suh ◽  
F. Afaq ◽  
N. Khan ◽  
J. J. Johnson ◽  
F. H. Khusro ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cell Death ◽  
Cancer Cells ◽  
Signaling Pathway ◽  
Mtor Signaling ◽  
Autophagic Cell Death ◽  
Mtor Signaling Pathway ◽  
Prostate Cancer Cells
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