High-Energy Shock Waves Alter Cytosolic Calcium Mobilization in Single MDCK Cells

The existence of non thermal radio bursts provide evidences for the acceleration of electrons in the solar atmosphere.It is shown, from the characteristics of the bursts, that the electrons are accelerated in at least four different phases: (1)An impulsive phase which gives μib and III bursts.(2)A gradual phase which gives μIV and S1IV bursts.(3)A quasi-continuous phase which gives S2IV bursts and noise storms.(4)An acceleration by shock waves gives type II bursts.(5)Eventually, another shock-wave acceleration giving the moIV burst.

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In Vivo Effects of High Energy Shock Waves on Urological Tumors: An Evaluation of Treatment Modalities

The Journal of Urology ◽

10.1016/s0022-5347(17)39592-7 ◽

1990 ◽

Vol 144 (3) ◽

pp. 785-789 ◽

Cited By ~ 37

Author(s):

Gosse O.N. Oosterhof ◽

Geert A.H.J. Smits ◽

Anja E. de Ruyter ◽

Jack A. Schalken ◽

Frans M.J. Debruyne

Keyword(s):

Shock Waves ◽

High Energy ◽

Treatment Modalities ◽

In Vivo Effects ◽

Urological Tumors

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Fricke Dosimetric Analysis of the Radiation Ionization Equivalence of High Energy Shock Waves

The Journal of Urology ◽

10.1016/s0022-5347(17)75857-0 ◽

1987 ◽

Vol 137 (6) ◽

Author(s):

Thomas R. Morgan ◽

Vincent P. Laudone

Keyword(s):

Shock Waves ◽

High Energy ◽

Dosimetric Analysis

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High energy shock waves in the treatment of delayed and nonunion of fractures

International Orthopaedics ◽

10.1007/bf00192289 ◽

1991 ◽

Vol 15 (3) ◽

Cited By ~ 158

Author(s):

V.D. Valchanou ◽

P. Michailov

Keyword(s):

Shock Waves ◽

High Energy

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Calcium Regulates the Association between Mitochondria and a Smooth Subdomain of the Endoplasmic Reticulum

The Journal of Cell Biology ◽

10.1083/jcb.150.6.1489 ◽

2000 ◽

Vol 150 (6) ◽

pp. 1489-1498 ◽

Cited By ~ 111

Author(s):

Hui-Jun Wang ◽

Ginette Guay ◽

Liviu Pogan ◽

Remy Sauvé ◽

Ivan R. Nabi

Keyword(s):

Confocal Microscopy ◽

Mdck Cells ◽

Close Association ◽

Cytosolic Calcium ◽

Free Calcium ◽

Autocrine Motility Factor ◽

Cytosolic Free Calcium ◽

Motility Factor ◽

Rat Liver Cytosol ◽

Close Contacts

Association between the ER and mitochondria has long been observed, and the formation of close contacts between ER and mitochondria is necessary for the ER-mediated sequestration of cytosolic calcium by mitochondria. Autocrine motility factor receptor (AMF-R) is a marker for a smooth subdomain of the ER, shown here by confocal microscopy to be distinct from, yet closely associated with the calnexin- or calreticulin-labeled ER. By EM, smooth ER AMF-R tubules exhibit direct interactions with mitochondria, identifying them as a mitochondria-associated smooth ER subdomain. In digitonin-permeabilized MDCK cells, the addition of rat liver cytosol stimulates the dissociation of smooth ER and mitochondria under conditions of low calcium. Using BAPTA chelators of various affinities and CaEGTA buffers of defined free Ca2+ concentrations and quantitative confocal microscopy, we show that free calcium concentrations <100 nM favor dissociation, whereas those >1 μM favor close association between these two organelles. Therefore, we describe a cellular mechanism that facilitates the close association of this smooth ER subdomain and mitochondria when cytosolic free calcium rises above physiological levels.

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Amelioration of ischemic calcium overload correlates with high-energy phosphates in senescent myocardium

AJP Heart and Circulatory Physiology ◽

10.1152/ajpheart.1997.273.1.h418 ◽

1997 ◽

Vol 273 (1) ◽

pp. H418-H425 ◽

Cited By ~ 13

Author(s):

T. Tsukube ◽

J. D. McCully ◽

K. R. Metz ◽

C. U. Cook ◽

S. Levitsky

Keyword(s):

Functional Recovery ◽

Cytosolic Calcium ◽

High Energy ◽

Left Ventricular ◽

Global Ischemia ◽

Nucleoside Triphosphate ◽

Mrna Levels ◽

High Energy Phosphates ◽

Surgically Induced ◽

Free Magnesium

Previously, we have shown that potassium and magnesium (K-Mg, 20 mM each) cardioplegia ameliorated cytosolic calcium ([Ca2+]i) accumulation and was associated with enhanced functional recovery after surgically induced global ischemia in the aged heart. K-Mg cardioplegia was also shown to enhance cytosolic cytochrome oxidase I activity and mRNA levels, suggesting that enhanced functional recovery may involve the preservation of high-energy phosphates. To investigate this hypothesis, 31P nuclear magnetic resonance was used to measure serial alterations in phosphocreatine (PCr), inorganic phosphate, nucleoside triphosphate (NTP), intracellular free magnesium (Mgf), and intracellular pH (pHi) in Langendorff-perfused, aged (135 wk) rabbit hearts during preischemia, global ischemia (30 min), and reperfusion (30 min). K-Mg cardioplegia retarded PCr depletion (P <0.05) and significantly enhanced NTP preservation (P < 0.05) during ischemia and reperfusion. K-Mg cardioplegia also attenuated the increase in Mgf during ischemia (P < 0.05). These results were correlated with amelioration of [Ca2+]i accumulation during ischemia and preservation of left ventricular function after reperfusion and suggest that optimal functional recovery from surgically induced ischemia is provided by K-Mg cardioplegia in the aged myocardium.

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