Review – 3D Micro CT Imaging of Renal Micro-Structural Changes

2006 ◽  
Vol 103 (2) ◽  
pp. c66-c70 ◽  
Author(s):  
Mario Gössl ◽  
Michael D. Bentley ◽  
Lilach O. Lerman
Keyword(s):  
Structural Changes ◽  
Ct Imaging ◽  
Micro Ct

scholarly journals Dual Blockade of TNF and IL-17A Inhibits Inflammation and Structural Damage in a Rat Model of Spondyloarthritis

2022 ◽  
Vol 23 (2) ◽  
pp. 859
Author(s):  
Ihsan Hammoura ◽  
Renee H. Fiechter ◽  
Shaughn H. Bryant ◽  
Susan Westmoreland ◽  
Gillian Kingsbury ◽  
...  
Keyword(s):  
Bone Formation ◽  
Rat Model ◽  
Structural Damage ◽  
Structural Changes ◽  
Ct Imaging ◽  
Control Treatment ◽  
Micro Ct ◽  
New Bone Formation ◽  
Dual Blockade ◽  
Dual Inhibition

The tumor necrosis factor (TNF) and IL-23/IL-17 axes are the main therapeutic targets in spondyloarthritis. Despite the clinical efficacy of blocking either pathway, monotherapy does not induce remission in all patients and its effect on new bone formation remains unclear. We aimed to study the effect of TNF and IL-17A dual inhibition on clinical disease and structural damage using the HLA-B27/human β2-microglobulin transgenic rat model of SpA. Immunized rats were randomized according to arthritis severity, 1 week after arthritis incidence reached 50%, to be treated twice weekly for a period of 5 weeks with either a dual blockade therapy of an anti-TNF antibody and an anti-IL-17A antibody, a single therapy of either antibody, or PBS as vehicle control. Treatment-blinded observers assessed inflammation and structural damage clinically, histologically and by micro-CT imaging. Both single therapies as well as TNF and IL-17A dual blockade therapy reduced clinical spondylitis and peripheral arthritis effectively and similarly. Clinical improvement was confirmed for all treatments by a reduction of histological inflammation and pannus formation (p < 0.05) at the caudal spine. All treatments showed an improvement of structural changes at the axial and peripheral joints on micro-CT imaging, with a significant decrease for roughness (p < 0.05), which reflects both erosion and new bone formation, at the level of the caudal spine. The effect of dual blockade therapy on new bone formation was more prominent at the axial than the peripheral level. Collectively, our study showed that dual blockade therapy significantly reduces inflammation and structural changes, including new bone formation. However, we could not confirm a more pronounced effect of dual inhibition compared to single inhibition.

scholarly journals Nondestructive cryomicro-CT imaging enables structural and molecular analysis of human lung tissue

2017 ◽  
Vol 122 (1) ◽  
pp. 161-169 ◽  
Author(s):  
Dragoş M. Vasilescu ◽  
André B. Phillion ◽  
Naoya Tanabe ◽  
Daisuke Kinose ◽  
David F. Paige ◽  
...  
Keyword(s):  
Molecular Analysis ◽  
Structural Changes ◽  
Human Lung ◽  
Three Dimensional ◽  
Ct Imaging ◽  
Matrix Composition ◽  
Micro Ct ◽  
Micro Computed Tomography ◽  
Human Lung Tissue ◽  
Lung Structure

Micro-computed tomography (CT) enables three-dimensional (3D) imaging of complex soft tissue structures, but current protocols used to achieve this goal preclude cellular and molecular phenotyping of the tissue. Here we describe a radiolucent cryostage that permits micro-CT imaging of unfixed frozen human lung samples at an isotropic voxel size of (11 µm)3 under conditions where the sample is maintained frozen at −30°C during imaging. The cryostage was tested for thermal stability to maintain samples frozen up to 8 h. This report describes the methods used to choose the materials required for cryostage construction and demonstrates that whole genome mRNA integrity and expression are not compromised by exposure to micro-CT radiation and that the tissue can be used for immunohistochemistry. The new cryostage provides a novel method enabling integration of 3D tissue structure with cellular and molecular analysis to facilitate the identification of molecular determinants of disease. NEW & NOTEWORTHY The described micro-CT cryostage provides a novel way to study the three-dimensional lung structure preserved without the effects of fixatives while enabling subsequent studies of the cellular matrix composition and gene expression. This approach will, for the first time, enable researchers to study structural changes of lung tissues that occur with disease and correlate them with changes in gene or protein signatures.

scholarly journals Micro-CT imaging of Thiel-embalmed and iodine-stained human temporal bone for 3D modeling

2021 ◽  
Vol 50 (1) ◽  
Author(s):  
Sebastian Halm ◽  
David Haberthür ◽  
Elisabeth Eppler ◽  
Valentin Djonov ◽  
Andreas Arnold
Keyword(s):  
Soft Tissue ◽  
Temporal Bone ◽  
Middle Ear ◽  
3D Modeling ◽  
Soft Tissues ◽  
Ct Imaging ◽  
Micro Ct ◽  
Data Set ◽  
Iodine Staining ◽  
Tissue Structures

Abstract Introduction This pilot study explores whether a human Thiel-embalmed temporal bone is suitable for generating an accurate and complete data set with micro-computed tomography (micro-CT) and whether solid iodine-staining improves visualization and facilitates segmentation of middle ear structures. Methods A temporal bone was used to verify the accuracy of the imaging by first digitally measuring the stapes on the tomography images and then physically under the microscope after removal from the temporal bone. All measurements were compared with literature values. The contralateral temporal bone was used to evaluate segmentation and three-dimensional (3D) modeling after iodine staining and micro-CT scanning. Results The digital and physical stapes measurements differed by 0.01–0.17 mm or 1–19%, respectively, but correlated well with the literature values. Soft tissue structures were visible in the unstained scan. However, iodine staining increased the contrast-to-noise ratio by a factor of 3.7 on average. The 3D model depicts all ossicles and soft tissue structures in detail, including the chorda tympani, which was not visible in the unstained scan. Conclusions Micro-CT imaging of a Thiel-embalmed temporal bone accurately represented the entire anatomy. Iodine staining considerably increased the contrast of soft tissues, simplified segmentation and enabled detailed 3D modeling of the middle ear.

Integrating micro CT indices, CT imaging and computational modelling to assess the mechanical performance of fluoride treated bone

2013 ◽  
Vol 35 (12) ◽  
pp. 1793-1800 ◽  
Author(s):  
D. Sreenivasan ◽  
M. Watson ◽  
K. Callon ◽  
M. Dray ◽  
R. Das ◽  
...  
Keyword(s):  
Mechanical Performance ◽  
Computational Modelling ◽  
Ct Imaging ◽  
Micro Ct

Quantitative 3D Micro-CT Imaging of the Human Feto-placental Vasculature in Intrauterine Growth Restriction

Placenta ◽  
2008 ◽  
Vol 29 (11) ◽  
pp. 937-941 ◽  
Author(s):  
A.C. Langheinrich ◽  
S. Vorman ◽  
J. Seidenstücker ◽  
M. Kampschulte ◽  
R.M. Bohle ◽  
...  
Keyword(s):  
Intrauterine Growth Restriction ◽  
Ct Imaging ◽  
Growth Restriction ◽  
Micro Ct ◽  
Intrauterine Growth

scholarly journals Micro-CT data of early physiological cancellous bone formation in the lumbar spine of female C57BL/6 mice

2021 ◽  
Vol 8 (1) ◽  
Author(s):  
Michael Zenzes ◽  
Paul Zaslansky
Keyword(s):  
Cancellous Bone ◽  
Structural Changes ◽  
Bone Growth ◽  
Post Partum ◽  
Three Dimensional ◽  
Lumbar Vertebra ◽  
Tissue Growth ◽  
Micro Ct ◽  
Mineral Density ◽  
Ct Data

AbstractMicro-CT provides critical data for musculoskeletal research, yielding three-dimensional datasets containing distributions of mineral density. Using high-resolution scans, we quantified changes in the fine architecture of bone in the spine of young mice. This data is made available as a reference to physiological cancellous bone growth. The scans (n = 19) depict the extensive structural changes typical for female C57BL/6 mice pups, aged 1-, 3-, 7-, 10- and 14-days post-partum, as they attain the mature geometry. We reveal the micro-morphology down to individual trabeculae in the spine that follow phases of mineral-tissue rearrangement in the growing lumbar vertebra on a micrometer length scale. Phantom data is provided to facilitate mineral density calibration. Conventional histomorphometry matched with our micro-CT data on selected samples confirms the validity and accuracy of our 3D scans. The data may thus serve as a reference for modeling normal bone growth and can be used to benchmark other experiments assessing the effects of biomaterials, tissue growth, healing, and regeneration.

scholarly journals Micro-CT Imaging of Tumor Angiogenesis

2014 ◽  
Vol 184 (2) ◽  
pp. 431-441 ◽  
Author(s):  
Josef Ehling ◽  
Benjamin Theek ◽  
Felix Gremse ◽  
Sarah Baetke ◽  
Diana Möckel ◽  
...  
Keyword(s):  
Tumor Angiogenesis ◽  
Ct Imaging ◽  
Micro Ct
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