Comparison of Short Term Indirect Calorimetry and Doubly Labeled Water Method for the Assessment of Energy Expenditure in Preterm Infants

The doubly labeled water (2H(2)18O) method used to estimate total energy expenditure (EETotal) is particularly sensitive to analytic error in preterm infants, because of their high percentage of body water and the high ratio of water flux to CO2 production. To evaluate further use of this method, the EE of 12 preterm infants was measured by indirect calorimetry and 2H(2)18O simultaneously and continuously for 5 days. Initial infant weight, age, and postconceptional age were (means +/- SD) 1,674 +/- 173 g, 4.4 +/- 2.6 wk, and 34.6 +/- 1.6 wk, respectively. The indirect calorimeter system included an air-temperature-controlled chamber and heart rate monitor. EE was measured by indirect calorimetry for 85.6 +/- 4.7% of study time and estimated from the linear regression of heart rate on EE for 14.4 +/- 4.7% of study time. The 2H(2)18O method entailed an initial dose of 100 mg 2H2O and 250 mg 18O/kg and a final dose of 75 mg 18O/kg; urine was collected twice daily. 2H and 18O enrichments were measured by gas-isotope-ratio mass spectrometry. EE was calculated from measured 2H and 18O dilution spaces (NH, NO), turnover rates (kH, kO), and measured respiratory quotient. The ratio of 2H to 18O dilution spaces was 1.01 +/- 0.01 and the ratio of kO to kH was 1.16 +/- 0.04. Estimation of EE from 2H(2)18O and indirect calorimetry agreed within 1%, although individual variability in methods was large.

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Energy expenditure determined by the doubly labeled water method in Bolivian Aymara living in a high altitude agropastoral community

American Journal of Clinical Nutrition ◽

10.1093/ajcn/62.5.901 ◽

1995 ◽

Vol 62 (5) ◽

pp. 901-910 ◽

Cited By ~ 41

Author(s):

H Kashiwazaki ◽

Y Dejima ◽

J Orias-Rivera ◽

W A Coward

Keyword(s):

Energy Expenditure ◽

High Altitude ◽

Doubly Labeled Water ◽

Doubly Labeled Water Method

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Methodological error in measurement of energy expenditure by the doubly labeled water method: much ado about nothing?

American Journal of Clinical Nutrition ◽

10.1093/ajcn/nqz156 ◽

2019 ◽

Vol 110 (5) ◽

pp. 1253-1254 ◽

Cited By ~ 1

Author(s):

David S Ludwig ◽

Cara B Ebbeling ◽

Julia M W Wong ◽

Robert R Wolfe ◽

William W Wong

Keyword(s):

Energy Expenditure ◽

Methodological Error ◽

Doubly Labeled Water ◽

Much Ado About Nothing ◽

Doubly Labeled Water Method

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Use of doubly labeled water technique in soldiers training for jungle warfare

Journal of Applied Physiology ◽

10.1152/jappl.1989.67.1.14 ◽

1989 ◽

Vol 67 (1) ◽

pp. 14-18 ◽

Cited By ~ 34

Author(s):

C. H. Forbes-Ewan ◽

B. L. Morrissey ◽

G. C. Gregg ◽

D. R. Waters

Keyword(s):

Energy Balance ◽

Food Intake ◽

Energy Expenditure ◽

Daily Intake ◽

Doubly Labeled Water ◽

Daily Energy Expenditure ◽

Balance Study ◽

Wide Range ◽

Daily Energy ◽

Doubly Labeled Water Method

The doubly labeled water method was used to estimate the energy expended by four members of an Australian Army platoon (34 soldiers) engaged in training for jungle warfare. Each subject received an oral isotope dose sufficient to raise isotope levels by 200–250 (18O) and 100–120 ppm (2H). The experimental period was 7 days. Concurrently, a factorial estimate of the energy expenditure of the platoon was conducted. Also, a food intake-energy balance study was conducted for the platoon. Mean daily energy expenditure by the doubly labeled water method was 4,750 kcal (range 4,152–5,394 kcal). The factorial estimate of mean daily energy expenditure was 4,535 kcal. Because of inherent inaccuracies in the food intake-energy balance technique, we were able to conclude only that energy expenditure, as measured by this method, was greater than the estimated mean daily intake of 4,040 kcal. The doubly labeled water technique was well tolerated, is noninvasive, and appears to be suitable in a wide range of field applications.

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