Effect of Low Oxygen Tension on the Growth of Bovine Corneal Endothelial Cells in vitro

1989 ◽  
Vol 21 (6) ◽  
pp. 440-442 ◽  
Author(s):  
Z. Zagórski ◽  
B. Gossler ◽  
G.O.H. Naumann
Keyword(s):  
Endothelial Cells ◽  
Oxygen Tension ◽  
Corneal Endothelial Cells ◽  
Low Oxygen ◽  
Low Oxygen Tension

Enhanced Proliferation and Functions of In Vitro Expanded Human Hair Follicle Outer Root Sheath Cells by Low Oxygen Tension Culture

2012 ◽  
Vol 18 (8) ◽  
pp. 603-613 ◽  
Author(s):  
Lingling Xia ◽  
Qing Liu ◽  
Wenjie Zhang ◽  
Guangdong Zhou ◽  
Yilin Cao ◽  
...  
Keyword(s):  
Hair Follicle ◽  
Oxygen Tension ◽  
Human Hair ◽  
Low Oxygen ◽  
Human Hair Follicle ◽  
Outer Root Sheath ◽  
Root Sheath ◽  
Low Oxygen Tension ◽  
Outer Root Sheath Cells

247 EFFECTS OF CYSTEINE IN IVM MEDIA ON IN VITRO MATURATION UNDER LOW OXYGEN TENSION, IN VITRO FERTILIZATION, AND IN VITRO CULTURE OF PORCINE OOCYTES

2008 ◽  
Vol 20 (1) ◽  
pp. 203
Author(s):  
N. V. Linh ◽  
D. N. Q. Thanh ◽  
M. Ozawa ◽  
B. X. Nguyen ◽  
K. Kikuchi ◽  
...  
Keyword(s):  
Oxygen Tension ◽  
Blastocyst Stage ◽  
Low Oxygen ◽  
In Vitro Production ◽  
Vitro Fertilization ◽  
Nuclear Maturation ◽  
Low Oxygen Tension ◽  
High O2 ◽  
Group 1

Cysteine is considered to promote male pronuclear (MPN) formation in porcine through oocyte glutathione (GSH) synthesis (Yoshida et al. 1993 Biol. Reprod. 49, 89–94). The GSH has an important role in providing cells with a redox state and in acting to protect cells from toxic effects of oxidative damage (Meister et al. 1976 AM Rev. Biochem. 45, 559–604). However, such previous investigations were carried out under high O2 tension (20% O2) incubation conditions. Here we simply study IVM-IVF-IVC competence of porcine oocytes matured in IVM media supplemented with cysteine of different concentrations under low oxygen tension (5% O2). Cumulus–oocyte complexes (COCs) from prepubertal gilts were collected, matured, and fertilized in vitro according to Kikuchi et al. (2000 Biol. Reprod. 66, 1033–1041). COCs were cultured in IVM medium supplemented with 0 (Group 1; control), 0.05 (Group 2), 0.1 (Group 3), 0.2 (Group 4), and 0.6 mm (Group 5) cysteine under low oxygen tension. Nuclear maturation of oocytes, fertilization status, and number of cells in resultant embryos were assessed with orcein staining; also, the GSH content of IVM oocytes was measured by the method described by Ozawa et al. (2002 Reproduction 124, 683–689). Maturation rates of Groups 1–5 were 68.2 � 3.2, 70.6 � 7.7, 69.7 � 15.9, 75.9 � 7.7, and 68.8 � 8.0%, respectively, indicating no difference in maturation competence among the groups (P > 0.05 by ANOVA). The rates of sperm penetration, MPN formation (95.9 � 2.4, 100 � 0, 92.8 � 4.7, 94.0 � 4.1, and 92.4 � 2.7%, respectively), monospermy, and even blastocyst rates after 6 days of IVC were not different among the groups (P > 0.05 by ANOVA). Moreover, the cell numbers of blastomeres in blastocysts (38.68 � 3.5, 40.1 � 3.1, 37.5 � 3.0, 36.2 � 3.3, and 43.8 � 4.0, respectively) were uniformly the same among the groups (P > 0.05 by ANOVA). However, GSH content of IVM oocytes increased significantly (P < 0.05 by ANOVA) as the concentration of cysteine increased (12.2 � 0.6, 14 � 0.8, 15.1 � 0.5, 16.4 � 0.4, and 16.4 � 0.5 pmol/oocyte, respectively). The GSH level of oocytes in Group 1 (control) seems to be higher than that reported by Aberydeera et al. (1998 Biol. Reprod. 58, 213–218), who matured porcine oocytes under high O2 tension. This may reflect the effect of low O2 tension and explain the same developmental rate to the blastocyst stage as that of oocytes matured in the media supplemented with cysteine in this study. In conclusion, an addition of 0.05–0.6 mm cysteine during IVM, under 5% O2 tension, of porcine oocytes significantly increased intracellular GSH synthesis according to its concentration. However, it had no promoting effects on nuclear maturation, fertilization, male pronucleus formation, and subsequent embryonic development to the blastocyst stage. Thus, O2 tension during IVM of oocytes is suggested to be important for the in vitro production of porcine blastocysts.

Embryo culture at a reduced oxygen concentration of 5%: a mini review

Zygote ◽  
2019 ◽  
Vol 27 (6) ◽  
pp. 355-361 ◽  
Author(s):  
R. Sciorio ◽  
G.D. Smith
Keyword(s):  
Oxygen Tension ◽  
Embryo Development ◽  
Atmospheric Oxygen ◽  
Embryo Implantation ◽  
Critical Role ◽  
Low Oxygen ◽  
Physiological Level ◽  
Low Oxygen Tension

SummaryThe optimum oxygen tension for culturing mammalian embryos has been widely debated by the scientific community. While several laboratories have moved to using 5% as the value for oxygen tension, the majority of modern in vitro fertilization (IVF) laboratory programmes still use 20%. Several in vivo studies have shown the oxygen tension measured in the oviduct of mammals fluctuates between 2% and 8% and in cows and primates this values drops to <2% in the uterine milieu. In human IVF, a non-physiological level of 20% oxygen has been used in the past. However, several studies have shown that atmospheric oxygen introduces adverse effects to embryo development, not limited to numerous molecular and cellular physiology events. In addition, low oxygen tension plays a critical role in reducing the high level of detrimental reactive oxygen species within cells, influences embryonic gene expression, helps with embryo metabolism of glucose, and enhances embryo development to the blastocyst stage. Collectively, this improves embryo implantation potential. However, clinical studies have yielded contradictory results. In almost all reports, some level of improvement has been identified in embryo development or implantation, without any observed drawbacks. This review article will examine the recent literature and discusses ongoing efforts to understand the benefits that low oxygen tension can bring to mammal embryo development in vitro.

Erythropoietin Signaling and Response in Vascular Smooth Muscle Cells.

Blood ◽  
2007 ◽  
Vol 110 (11) ◽  
pp. 3724-3724
Author(s):  
Bojana B. Beleslin-Cokic ◽  
Vladan P. Cokic ◽  
Ljiljana Gojkovic-Bukarica ◽  
Constance Tom Noguchi
Keyword(s):  
Endothelial Cells ◽  
Smooth Muscle ◽  
Blood Vessel ◽  
Vascular Smooth Muscle ◽  
Oxygen Tension ◽  
Erythropoietin Receptor ◽  
No Production ◽  
Low Oxygen ◽  
Erythroid Progenitor ◽  
Low Oxygen Tension

Abstract Erythopoietin (EPO) and erythropoietin receptor (EPOR) regulate survival, proliferation, differentiation and viability of erythroid progenitor cells. Beyond erythropoiesis, we have observed that human vascular endothelial cells respond to EPO stimulation by inducing EPOR and endothelial nitric oxide synthase (eNOS) expression, increasing NO production and cGMP, particularly under low oxygen tension. In this study, we investigate the response of vascular smooth muscle (VSM) to EPO stimulation and the contribution of blood vessel to relaxation/contraction. We found that VSM cells express EPOR and that treatment with EPO (5 U/ml) at reduced oxygen tension increased EPOR mRNA by 2 fold. This increased EPO responsiveness at low oxygen tension is accompanied by increased cell proliferation at 2% O2 more than 2 fold. Unlike endothelial cells, EPO did not induce eNOS or NO production in VSM cells. PI-3 kinase was involved in EPO stimulation with no change in MAP kinase. In an isolated blood vessel model system, we checked EPO responsiveness. EPO produced contractions (0.32 ± 0.3 g) of rat renal artery, and pretreatment with LY294002 (10 mM), an inhibitor of PI-3 kinase, statistically significantly inhibited this contraction (58.7 ± 7%). This response was also observed with the endothelium layer removed. In preparations of human internal mammary artery (HIMA) and human saphenous vein (HSV) from patients undergoing coronary artery bypass, EPO did not affect basal vascular tone of HIMA and HSV with the endothelium layer removed, but EPO (5 U/ml) potentated noradrenalin-induced contraction by up to 2 fold in HSV and HIMA. Also, pretreatment with EPO significantly increase angiotensin-evoked contractions of these blood vessels (50 ± 8%, 113 ± 17%, respectively P &lt; 0.01), suggesting that EPO has synergistic effects on angiotensin or noradrenalin-induced [Ca2+] mobilization, particularly on intracellular Ca2+ release. These data suggest that EPO stimulation of vascular smooth muscle may act to modulate or balance the vasodilatory effects of increased NO production by EPO stimulated endothelium. Under oxygen stress vascular smooth muscle can respond to EPO by proliferation and PI-3 kinase activation as a protective effect and in conjunction with Ca2+ release likely contributes to the overall vascular response.

Human Retinal Pigment Epithelium in vitro: Influence of Low Oxygen Tension, Glucose and Insulin

1993 ◽  
Vol 25 (4) ◽  
pp. 226-234 ◽  
Author(s):  
H.L.J. Knorr ◽  
M. Linde-Behringer ◽  
B. Gossler ◽  
U.M. Mayer
Keyword(s):  
Retinal Pigment Epithelium ◽  
Oxygen Tension ◽  
Pigment Epithelium ◽  
Retinal Pigment ◽  
Low Oxygen ◽  
Human Retinal Pigment Epithelium ◽  
Low Oxygen Tension

Reactive oxygen species-driven HIF1α triggers accelerated glycolysis in endothelial cells exposed to low oxygen tension

2017 ◽  
Vol 45 ◽  
pp. 8-14 ◽  
Author(s):  
Jin-Young Paik ◽  
Kyung-Ho Jung ◽  
Jin-Hee Lee ◽  
Jin-Won Park ◽  
Kyung-Han Lee
Keyword(s):  
Reactive Oxygen Species ◽  
Endothelial Cells ◽  
Oxygen Tension ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Low Oxygen ◽  
Low Oxygen Tension

scholarly journals Low Oxygen Tension Primes Aortic Endothelial Cells to the Reparative Effect of Tissue-Protective Cytokines

2015 ◽  
Vol 21 (1) ◽  
pp. 709-716 ◽  
Author(s):  
Lamia Heikal ◽  
Pietro Ghezzi ◽  
Manuela Mengozzi ◽  
Gordon Ferns
Keyword(s):  
Endothelial Cells ◽  
Oxygen Tension ◽  
Low Oxygen ◽  
Aortic Endothelial Cells ◽  
Low Oxygen Tension ◽  
Reparative Effect ◽  
Aortic Endothelial
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