Cytochemical Studies of Nuclear DNA and RNA in Normal and Abnormal Keratinizing Tissues*

2015 ◽  
pp. 127-140 ◽  
Author(s):  
Hiroyuki Suzuki ◽  
Mineko Hirano ◽  
Shunichi Baba
Keyword(s):  
Nuclear Dna ◽  
Dna And Rna

DNA-RNA complexes that might represent transient attachment sites of nuclear DNA to the matrix

1990 ◽  
Vol 95 (4) ◽  
pp. 667-674
Author(s):  
C. Patriotis ◽  
M. Andreeva ◽  
M. Pascaleva ◽  
V. Ivanov ◽  
L. Djondjurov
Keyword(s):  
Dna Sequences ◽  
Nuclear Dna ◽  
Rna Polymerase Iii ◽  
Erythroid Differentiation ◽  
Rnase H ◽  
Dna And Rna ◽  
Attachment Sites ◽  
The Matrix ◽  
Exo Iii ◽  
Rna Complexes

In this study we describe DNA-RNA complexes in matrix DNA of Friend cells. The presence of such unusual structures is confirmed by the following evidence. When a preparation of matrix DNA is electrophoresed in agarose an RNA component always migrates together with DNA. There should be a close interaction between DNA and RNA in such a preparation because the presence of the RNA component causes resistance of DNA to DNase I and Exo III. An intimate, hybrid-type association of part of the RNA component with DNA is indicated also by the fact that about 20% of this RNA is sensitive to RNase H. By specific inhibition of the RNA synthesis with alpha-amanitin and actinomycin D it was shown that the bulk of associated RNA is transcribed by RNA polymerase III. Hybridization experiments showed similarity between the DNA sequences isolated from the complexes and those from the base of dehistonized DNA loops obtained by high-salt extraction of nuclei. This observation suggests that the complexes might represent attachment sites of nuclear DNA to the matrix: possibly, the attachment is mediated via the RNA component. Experiments with induction of erythroid differentiation indicated that a profound reorganization of the nucleus, accompanying terminal differentiation, leads to a striking reduction in the number of complexes and thus in the number of attachment sites. This suggests that the complexes should function as transient attachment sites.

Multiple Functions of Nuclear DNA Helicase II (RNA Helicase A) in Nucleic Acid Metabolism

2004 ◽  
Vol 36 (3) ◽  
pp. 177-183 ◽  
Author(s):  
Suisheng Zhang ◽  
Frank Grosse
Keyword(s):  
Nuclear Dna ◽  
Rna Helicase ◽  
Dna Helicase ◽  
Nucleic Acid Metabolism ◽  
Rna Helicase A ◽  
Dna Helicase Ii ◽  
Dna And Rna ◽  
Sequence Homologies ◽  
Drosophila Protein ◽  
Purification Methods

Abstract Nuclear DNA helicase II (NDH II), or RNA helicase A (RHA), was initially discovered in mammals by conventional protein purification methods. Molecular cloning identified apparent sequence homologies between NDH II and a Drosophila protein named maleless (MLE), the latter being essential for the Drosophila X-chromosome dosage compensation. Increasing amounts of evidence suggest that NDH II is involved in multiple aspects of cellular and viral DNA and RNA metabolism. Moreover the functions of NDH II may have potential clinical implications related to viral infection, autoimmune diseases, or even tumorigenesis.

Isolation and characterization of a Chlamydomonas reinhardtii mutant lacking the gamma-subunit of chloroplast coupling factor 1 (CF1)

1991 ◽  
Vol 11 (10) ◽  
pp. 5053-5058
Author(s):  
E J Smart ◽  
B R Selman
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Nuclear Dna ◽  
Coupling Factor ◽  
Dna Encoding ◽  
Gamma Subunit ◽  
Exogenous Dna ◽  
Dna And Rna ◽  
Isolation And Characterization ◽  
Chloroplast Coupling Factor ◽  
Chloroplast Coupling Factor 1

Several nonphotoautotrophic mutants of Chlamydomonas reinhardtii were generated by transforming strain nit1-305 (cw 15) with exogenous DNA. An enrichment for potential photophosphorylation mutants was performed on medium containing arsenate, and acetate-requiring auxotrophs were then identified by replica plating. Strains containing a potential mutation in the nuclear DNA encoding the chloroplast coupling factor 1 (CF1) gamma-subunit (the atpC gene) were first identified serologically with a monospecific antiserum directed against the CF1 gamma-subunit polypeptide. Of several mutants isolated, one, designated T1-54, was characterized at the protein, DNA, and RNA levels. Mutant strain T1-54 lacks anti-CF1 gamma-subunit cross-reacting material, exhibits polymorphism at the atpC locus compared with the parental strain, and lacks the mRNA transcript for the CF1 gamma-subunit. The data are consistent with there being an insertion of exogenous DNA, a deletion of DNA, or both at the 5' end of the gene encoding the CF1 gamma-subunit.

Infrared Spectroscopy of Human Cells and Tissue. Part VII: FT-IR Microspectroscopy of DNase- and RNase-Treated Normal, Cirrhotic, and Neoplastic Liver Tissue

2000 ◽  
Vol 54 (4) ◽  
pp. 480-485 ◽  
Author(s):  
Luis Chiriboga ◽  
Herman Yee ◽  
Max Diem
Keyword(s):  
Nuclear Dna ◽  
Spectral Features ◽  
Protein Matrix ◽  
Neoplastic Tissue ◽  
Tissue Samples ◽  
Spectral Changes ◽  
Disease States ◽  
Dna And Rna ◽  
Cytoplasmic Rna ◽  
Infrared Spectral

In our efforts to understand the infrared spectral features of cells and tissues, and the spectral changes occurring between normal and disease states, we reported previously a detailed correlation between histochemical/immunohistochemical and spectral results. These results suggested an increase of nucleic acid spectral contributions in neoplastic, as compared to normal, tissue samples. In the present paper, these studies are extended to report the spectral features of DNA and RNA separately in these tissue samples. This was accomplished by selectively digesting either DNA or RNA from tissue sections, leaving behind the protein matrix with nuclear/cytoplasmic RNA or the protein matrix with nuclear DNA, respectively. These results demonstrate that the spectral changes between normal and neoplastic tissue are mostly due to an enhanced signature of DNA in neoplastic tissue. This enhancement is sufficiently large to suggest that it is most likely due to an increased detectability of DNA, rather than an increase in concentration.

Changes in nuclear DNA and RNA during epidermal keratinization

1977 ◽  
Vol 184 (2) ◽  
Author(s):  
Hiroyuki Suzuki ◽  
Kimie Fukuyama ◽  
WilliamL. Epstein
Keyword(s):  
Nuclear Dna ◽  
Dna And Rna

scholarly journals Methods for simultaneous and quantitative isolation of mitochondrial DNA, nuclear DNA and RNA from mammalian cells

BioTechniques ◽  
2020 ◽  
Vol 69 (6) ◽  
pp. 436-442
Author(s):  
Jean Nakhle ◽  
Tülin Özkan ◽  
Kateřina Lněničková ◽  
Philippe Briolotti ◽  
Marie-Luce Vignais
Keyword(s):  
Stem Cells ◽  
Mitochondrial Dna ◽  
Cell Line ◽  
Mammalian Cells ◽  
Nuclear Dna ◽  
Housekeeping Genes ◽  
Cell Types ◽  
Hepg2 Cell Line ◽  
Primary Glioblastoma ◽  
Dna And Rna

The aim of this study was to assess two protocols for their capacities to simultaneously isolate RNA, mtDNA and ncDNA from mammalian cells. We compared the Invitrogen TRIzol-based method and Qiagen DNeasy columns, using the HepG2 cell line and human primary glioblastoma stem cells. Both methods allowed the isolation of all three types of nucleic acids and provided similar yields in mtDNA. However, the yield in ncDNA was more than tenfold higher on columns, as observed for both cell types. Conversely, the TRIzol method proved more reproducible and was the method of choice for isolating RNA from glioblastoma cells, as demonstrated for the housekeeping genes RPLP0 and RPS9.

scholarly journals Nuclear DNA and RNA amounts in the wild and cultivated urd and mung beans and their M1 plants.

CYTOLOGIA ◽  
1988 ◽  
Vol 53 (3) ◽  
pp. 535-541 ◽  
Author(s):  
S. Ignacimuthu ◽  
C. R. Babu
Keyword(s):  
Nuclear Dna ◽  
Dna And Rna ◽  
In The Wild

scholarly journals Isolation and characterization of a Chlamydomonas reinhardtii mutant lacking the gamma-subunit of chloroplast coupling factor 1 (CF1).

1991 ◽  
Vol 11 (10) ◽  
pp. 5053-5058 ◽  
Author(s):  
E J Smart ◽  
B R Selman
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Nuclear Dna ◽  
Coupling Factor ◽  
Dna Encoding ◽  
Gamma Subunit ◽  
Exogenous Dna ◽  
Dna And Rna ◽  
Isolation And Characterization ◽  
Chloroplast Coupling Factor ◽  
Chloroplast Coupling Factor 1

Several nonphotoautotrophic mutants of Chlamydomonas reinhardtii were generated by transforming strain nit1-305 (cw 15) with exogenous DNA. An enrichment for potential photophosphorylation mutants was performed on medium containing arsenate, and acetate-requiring auxotrophs were then identified by replica plating. Strains containing a potential mutation in the nuclear DNA encoding the chloroplast coupling factor 1 (CF1) gamma-subunit (the atpC gene) were first identified serologically with a monospecific antiserum directed against the CF1 gamma-subunit polypeptide. Of several mutants isolated, one, designated T1-54, was characterized at the protein, DNA, and RNA levels. Mutant strain T1-54 lacks anti-CF1 gamma-subunit cross-reacting material, exhibits polymorphism at the atpC locus compared with the parental strain, and lacks the mRNA transcript for the CF1 gamma-subunit. The data are consistent with there being an insertion of exogenous DNA, a deletion of DNA, or both at the 5' end of the gene encoding the CF1 gamma-subunit.

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