scholarly journals Nuclear DNA and RNA amounts in the wild and cultivated urd and mung beans and their M1 plants.

CYTOLOGIA ◽  
1988 ◽  
Vol 53 (3) ◽  
pp. 535-541 ◽  
Author(s):  
S. Ignacimuthu ◽  
C. R. Babu
Keyword(s):  
Nuclear Dna ◽  
Dna And Rna ◽  
In The Wild

Hydra collecting for citizen scientists v2

2021 ◽  
Author(s):  
Callen Hyland ◽  
Kimberly Sladek
Keyword(s):  
New York ◽  
Molecular Phylogenetics ◽  
Nuclear Dna ◽  
Model System ◽  
In The Wild ◽  
Submission Form ◽  
The University ◽  
To Receive ◽  
Business Media ◽  
Simple Materials

The freshwater cnidarian Hydra has been a model system for regeneration and developmental biology for over 250 years, but much remains unknown about their biodiversity and global distribution. As a citizen scientist, you can contribute to our understanding of Hydra in the wild by becoming a "Hydra Hunter". All it takes is a few simple materials and a little patience. Collecting Hydra in the wild can be challenging. You will certainly not find them everywhere you look. Keep in mind that NOT finding Hydra is still useful information because this will help us understand the environmental factors that effect their distribution. Metadata submission form: https://forms.gle/cAZCiiRCyE922G5t5 Please contact [email protected] for more information or to receive a Hydra collecting kit. Hydra collecting kits were purchased with a grant to Kimberly Sladek from the University of San Diego Associated Students Government. Thank you to Rob Steele for helpful feedback on this protocol. References: Campbell, R. D. (1983). Hydra Collecting. In H. M. Lenhoff (Ed.). Hydra: Research Methods. New York: Springer Science + Business Media. Martínez, D. E., et al. (2010). Phylogeny and biogeography of Hydra (Cnidaria: Hydridae) using mitochondrial and nuclear DNA sequence. Molecular Phylogenetics and Evolution, 57, 403-410. doi:10.1016/j.ympev.2010.06.016

DNA-RNA complexes that might represent transient attachment sites of nuclear DNA to the matrix

1990 ◽  
Vol 95 (4) ◽  
pp. 667-674
Author(s):  
C. Patriotis ◽  
M. Andreeva ◽  
M. Pascaleva ◽  
V. Ivanov ◽  
L. Djondjurov
Keyword(s):  
Dna Sequences ◽  
Nuclear Dna ◽  
Rna Polymerase Iii ◽  
Erythroid Differentiation ◽  
Rnase H ◽  
Dna And Rna ◽  
Attachment Sites ◽  
The Matrix ◽  
Exo Iii ◽  
Rna Complexes

In this study we describe DNA-RNA complexes in matrix DNA of Friend cells. The presence of such unusual structures is confirmed by the following evidence. When a preparation of matrix DNA is electrophoresed in agarose an RNA component always migrates together with DNA. There should be a close interaction between DNA and RNA in such a preparation because the presence of the RNA component causes resistance of DNA to DNase I and Exo III. An intimate, hybrid-type association of part of the RNA component with DNA is indicated also by the fact that about 20% of this RNA is sensitive to RNase H. By specific inhibition of the RNA synthesis with alpha-amanitin and actinomycin D it was shown that the bulk of associated RNA is transcribed by RNA polymerase III. Hybridization experiments showed similarity between the DNA sequences isolated from the complexes and those from the base of dehistonized DNA loops obtained by high-salt extraction of nuclei. This observation suggests that the complexes might represent attachment sites of nuclear DNA to the matrix: possibly, the attachment is mediated via the RNA component. Experiments with induction of erythroid differentiation indicated that a profound reorganization of the nucleus, accompanying terminal differentiation, leads to a striking reduction in the number of complexes and thus in the number of attachment sites. This suggests that the complexes should function as transient attachment sites.

scholarly journals Spatial Perturbations within an RNA Promoter Specifically Recognized by a Viral RNA-Dependent RNA Polymerase (RdRp) Reveal That RdRp Can Adjust Its Promoter Binding Sites

1999 ◽  
Vol 73 (1) ◽  
pp. 198-204 ◽  
Author(s):  
Scott Stevenson Stawicki ◽  
C. Cheng Kao
Keyword(s):  
Rna Polymerase ◽  
Rna Synthesis ◽  
Core Promoter ◽  
Brome Mosaic Virus ◽  
Viral Rna ◽  
Rna Dependent Rna Polymerase ◽  
Dna And Rna ◽  
In The Wild ◽  
Nucleotide Insertions

ABSTRACT RNA synthesis during viral replication requires specific recognition of RNA promoters by the viral RNA-dependent RNA polymerase (RdRp). Four nucleotides (−17, −14, −13, and −11) within the brome mosaic virus (BMV) subgenomic core promoter are required for RNA synthesis by the BMV RdRp (R. W. Siegel et al., Proc. Natl. Acad. Sci. USA 94:11238–11243, 1997). The spatial requirements for these four nucleotides and the initiation (+1) cytidylate were examined in RNAs containing nucleotide insertions and deletions within the BMV subgenomic core promoter. Spatial perturbations between nucleotides −17 and −11 resulted in decreased RNA synthesis in vitro. However, synthesis was still dependent on the key nucleotides identified in the wild-type core promoter and the initiation cytidylate. In contrast, changes between nucleotides −11 and +1 had a less severe effect on RNA synthesis but resulted in RNA products initiated at alternative locations in addition to the +1 cytidylate. The results suggest a degree of flexibility in the recognition of the subgenomic promoter by the BMV RdRp and are compared with functional regions in other DNA and RNA promoters.

Multiple Functions of Nuclear DNA Helicase II (RNA Helicase A) in Nucleic Acid Metabolism

2004 ◽  
Vol 36 (3) ◽  
pp. 177-183 ◽  
Author(s):  
Suisheng Zhang ◽  
Frank Grosse
Keyword(s):  
Nuclear Dna ◽  
Rna Helicase ◽  
Dna Helicase ◽  
Nucleic Acid Metabolism ◽  
Rna Helicase A ◽  
Dna Helicase Ii ◽  
Dna And Rna ◽  
Sequence Homologies ◽  
Drosophila Protein ◽  
Purification Methods

Abstract Nuclear DNA helicase II (NDH II), or RNA helicase A (RHA), was initially discovered in mammals by conventional protein purification methods. Molecular cloning identified apparent sequence homologies between NDH II and a Drosophila protein named maleless (MLE), the latter being essential for the Drosophila X-chromosome dosage compensation. Increasing amounts of evidence suggest that NDH II is involved in multiple aspects of cellular and viral DNA and RNA metabolism. Moreover the functions of NDH II may have potential clinical implications related to viral infection, autoimmune diseases, or even tumorigenesis.

Isolation and characterization of a Chlamydomonas reinhardtii mutant lacking the gamma-subunit of chloroplast coupling factor 1 (CF1)

1991 ◽  
Vol 11 (10) ◽  
pp. 5053-5058
Author(s):  
E J Smart ◽  
B R Selman
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Nuclear Dna ◽  
Coupling Factor ◽  
Dna Encoding ◽  
Gamma Subunit ◽  
Exogenous Dna ◽  
Dna And Rna ◽  
Isolation And Characterization ◽  
Chloroplast Coupling Factor ◽  
Chloroplast Coupling Factor 1

Several nonphotoautotrophic mutants of Chlamydomonas reinhardtii were generated by transforming strain nit1-305 (cw 15) with exogenous DNA. An enrichment for potential photophosphorylation mutants was performed on medium containing arsenate, and acetate-requiring auxotrophs were then identified by replica plating. Strains containing a potential mutation in the nuclear DNA encoding the chloroplast coupling factor 1 (CF1) gamma-subunit (the atpC gene) were first identified serologically with a monospecific antiserum directed against the CF1 gamma-subunit polypeptide. Of several mutants isolated, one, designated T1-54, was characterized at the protein, DNA, and RNA levels. Mutant strain T1-54 lacks anti-CF1 gamma-subunit cross-reacting material, exhibits polymorphism at the atpC locus compared with the parental strain, and lacks the mRNA transcript for the CF1 gamma-subunit. The data are consistent with there being an insertion of exogenous DNA, a deletion of DNA, or both at the 5' end of the gene encoding the CF1 gamma-subunit.

scholarly journals Integrating molecular, phenotypic and environmental data to elucidate patterns of crocodile hybridization in Belize

2015 ◽  
Vol 2 (9) ◽  
pp. 150409 ◽  
Author(s):  
Evon R. Hekkala ◽  
Steven G. Platt ◽  
John B. Thorbjarnarson ◽  
Thomas R. Rainwater ◽  
Michael Tessler ◽  
...  
Keyword(s):  
Nuclear Dna ◽  
Molecular Data ◽  
Environmental Data ◽  
Phenotypic Data ◽  
Crocodylus Acutus ◽  
Breeding Habitats ◽  
American Crocodile ◽  
In Captivity ◽  
In The Wild ◽  
Morelet's Crocodile

The genus Crocodylus comprises 12 currently recognized species, many of which can be difficult to differentiate phenotypically. Interspecific hybridization among crocodiles is known to occur in captivity and has been documented between some species in the wild. The identification of hybrid individuals is of importance for management and monitoring of crocodilians, many of which are Convention on International Trade in Endangered Species (CITES) listed. In this study, both mitochondrial and nuclear DNA markers were evaluated for their use in confirming a suspected hybrid zone between American crocodile ( Crocodylus acutus ) and Morelet’s crocodile ( Crocodylus moreletii ) populations in southern Belize where individuals and nests exhibiting atypical phenotypic features had previously been observed. Patterns observed in both phenotypic and molecular data indicate possible behavioural and ecological characteristics associated with hybridization events. The results of the combined analyses found that the majority of suspected hybrid samples represent crosses between female C. acutus and male C. moreletii . Phenotypic data could statistically identify hybrids, although morphological overlap between hybrids and C. moreletii reduced reliability of identification based solely on field characters. Ecologically, C. acutus was exclusively found in saline waters, whereas hybrids and C. moreletii were largely absent in these conditions. A hypothesized correlation between unidirectional hybridization and destruction of C. acutus breeding habitats warrants additional research.

Infrared Spectroscopy of Human Cells and Tissue. Part VII: FT-IR Microspectroscopy of DNase- and RNase-Treated Normal, Cirrhotic, and Neoplastic Liver Tissue

2000 ◽  
Vol 54 (4) ◽  
pp. 480-485 ◽  
Author(s):  
Luis Chiriboga ◽  
Herman Yee ◽  
Max Diem
Keyword(s):  
Nuclear Dna ◽  
Spectral Features ◽  
Protein Matrix ◽  
Neoplastic Tissue ◽  
Tissue Samples ◽  
Spectral Changes ◽  
Disease States ◽  
Dna And Rna ◽  
Cytoplasmic Rna ◽  
Infrared Spectral

In our efforts to understand the infrared spectral features of cells and tissues, and the spectral changes occurring between normal and disease states, we reported previously a detailed correlation between histochemical/immunohistochemical and spectral results. These results suggested an increase of nucleic acid spectral contributions in neoplastic, as compared to normal, tissue samples. In the present paper, these studies are extended to report the spectral features of DNA and RNA separately in these tissue samples. This was accomplished by selectively digesting either DNA or RNA from tissue sections, leaving behind the protein matrix with nuclear/cytoplasmic RNA or the protein matrix with nuclear DNA, respectively. These results demonstrate that the spectral changes between normal and neoplastic tissue are mostly due to an enhanced signature of DNA in neoplastic tissue. This enhancement is sufficiently large to suggest that it is most likely due to an increased detectability of DNA, rather than an increase in concentration.

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