Erythrocyte Membrane-Specific Antigens in Friend Virus-Induced Leukemia Cells1

Monolayers and planar "black" lipid membranes have been widely used as models for studying the structure and properties of biological membranes. Because of the lack of a suitable method to prepare these membranes for electron microscopic observation, their ultrastructure is so far not well understood. A method of forming molecular bilayers over the holes of fine mesh grids was developed by Hui et al. to study hydrated and unsupported lipid bilayers by electron diffraction, and to image phase separated domains by diffraction contrast. We now adapted the method of Pattus et al. of spreading biological membranes vesicles on the air-water interfaces to reconstitute biological membranes into unsupported planar films for electron microscopic study. hemoglobin-free human erythrocyte membrane stroma was prepared by hemolysis. The membranes were spreaded at 20°C on balanced salt solution in a Langmuir trough until a surface pressure of 20 dyne/cm was reached. The surface film was repeatedly washed by passing to adjacent troughs over shallow partitions (fig. 1).

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Lectin Binding to Human Breast Tumor Cells

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100090154 ◽

1976 ◽

Vol 34 ◽

pp. 34-35

Author(s):

Robert E. Nordquist ◽

J. Hill Anglin ◽

Michael P. Lerner

Keyword(s):

Carcinoma Cell ◽

Ricinus Communis ◽

Lectin Binding ◽

Low Frequency ◽

Breast Carcinoma Cell Line ◽

Human Breast ◽

Human Breast Tumor ◽

Duct Carcinoma ◽

Specific Antigens ◽

Ricin Agglutinin

A human breast carcinoma cell line (BOT-2) was derived from an infiltrating duct carcinoma (1). These cells were shown to have antigens that selectively bound antibodies from breast cancer patient sera (2). Furthermore, these tumor specific antigens could be removed from the living cells by low frequency sonication and have been partially characterized (3). These proteins have been shown to be around 100,000 MW and contain approximately 6% hexose and hexosamines. However, only the hexosamines appear to be available for lectin binding. This study was designed to use Concanavalin A (Con A) and Ricinus Communis (Ricin) agglutinin for the topagraphical localization of D-mannopyranosyl or glucopyranosyl and D-galactopyranosyl or DN- acetyl glactopyranosyl configurations on BOT-2 cell surfaces.

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Characterization of HLA-A*0201 restricted cytotoxic CD8+ T cells directed against ewing tumor specific antigens

Klinische Pädiatrie ◽

10.1055/s-0029-1222667 ◽

2009 ◽

Vol 221 (03) ◽

Author(s):

S Pirson ◽

U Thiel ◽

H Bernhard ◽

GHS Richter ◽

S Burdach

Keyword(s):

T Cells ◽

Cd8 T Cells ◽

Specific Antigens ◽

Ewing Tumor

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Adoptive Cell Therapy for Gastrointestinal Cancers

Digestive Disease Interventions ◽

10.1055/s-0040-1718902 ◽

2020 ◽

Vol 04 (04) ◽

pp. 345-350

Author(s):

Ryan J. Slovak ◽

Hyun S. Kim

Keyword(s):

Cell Therapy ◽

Clinical Research ◽

Solid Tumors ◽

Immune Cells ◽

Ex Vivo ◽

Adoptive Cell Therapy ◽

Hematologic Malignancies ◽

Gastrointestinal Cancers ◽

Gastrointestinal Malignancies ◽

Specific Antigens

AbstractThe reinfusion of autologous or allogeneic immune cells that have been educated and/or engineered ex vivo to respond to tumor-specific antigens is termed “adoptive cell therapy.” While adoptive cell therapy has made tremendous strides in the treatment of hematologic malignancies, its utilization for solid tumors has lagged somewhat behind. The purpose of this article is to concisely review the clinical research that has been done to investigate adoptive cell therapy as a treatment for gastrointestinal malignancies.

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