Karyological and Electron-Microscopic Studies in Myocardial Cells of Primates after Experimentally Induced Cardiac Hypertrophy

1977 ◽  
Vol 6 (6) ◽  
pp. 349-359 ◽  
Author(s):  
Peter Pfitzer ◽  
Hans-Jürgen Knieriem ◽  
Hagen D. Schulte
Keyword(s):  
Cardiac Hypertrophy ◽  
Myocardial Cells ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
Experimentally Induced

Electron microscopic studies on hepatic alkaline phosphatase in experimentally induced biliary obstruction of the rat

1980 ◽  
Vol 15 (6) ◽  
pp. 600-605 ◽  
Author(s):  
Makoto Kako ◽  
Gotaro Toda ◽  
Masao Torii ◽  
Hiroshi Kimura ◽  
Kazuhiko Miyake ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Biliary Obstruction ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
Experimentally Induced

Heavy Meromyosin Binding Studies of Myocardial Cells in Cardiac Lethal Mutant Salamanders (Ambystoma Meximanum

1975 ◽  
Vol 33 ◽  
pp. 540-541
Author(s):  
Larry F. Lemanski
Keyword(s):  
Heart Development ◽  
Recessive Gene ◽  
Genetic Mutation ◽  
Heart Beat ◽  
Myocardial Cells ◽  
Binding Studies ◽  
Electron Microscopic ◽  
Lethal Mutant ◽  
Microscopic Studies ◽  
Gross Examination

A naturally-occurring genetic mutation, designated c for “cardiac lethal”, was discovered in Ambystoma meximanum. The effect of homozygosity for recessive gene c is the absence of a heart beat, even though initially heart development appears normal. Mutant embryos are first distinguishable form their normal siblings at Harrison stage 34, when the normals develop contracting hearts. The mutant hearts at this stage, upon gross examination appear structurally normal but fail to beat. Nevertheless, the mutants survive through stage 41, which is about 20 days beyond the heart-beat stage and exhibit normal swimming movements indicating that gene c does not affect skeletal muscle. Electron microscopic studies of normal hearts at stage 34 reveal that the myocardial cells contain organized myofibrils; these myofibrils first form directly beneath the plasma membrane. By stage 41, the normal myocardial cells contain numerous well-organized myofibrils and thus have now become highly differentiated muscle cells.

Surface morphology of Purkinje cells and myocardial cells following chemical digestion

1990 ◽  
Vol 48 (3) ◽  
pp. 420-421
Author(s):  
Tetsuo Morita ◽  
Tatsuo Shimada
Keyword(s):  
Aqueous Solution ◽  
Purkinje Cells ◽  
Morphological Characteristics ◽  
Tween 20 ◽  
Myocardial Cells ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Moderator Band ◽  
Microscopic Studies ◽  
Endocardial Endothelium

From light and transmission electron microscopic studies, it has been long known that Purkinje cells of mammalian hearts have morphological characteristics different from ordinary myocardial cells. In the present study, not only Purkinje cells and myocardial cells but also connective tissue sheaths surrounding these cells were investigated by combined scanning electron microscopy(SEM) and chemical digestion.The moderator band of adult sheep heart was used because it possessed both Purkinje cells and myocardial cells (Fig.1). Tissue blocks were immersed in Karnovsky’s fixative for 3hr or longer. Some fixed tissues were inrrtersed in a 3-5% aqueous solution of NaClO for 1 min to digest the endocardial endothelium, and then were treated with 8N HCl for 30 min at 60°C to remove connection tissue elements. The others were iirmersed in a 2N NaOH or KOH solution for 7 days at room temperature to digest cellular elements. After freeze-cracking in a 40% dimethyl sulfoxide solution, the tissues were washed throughout in a saline solution containing tween 20, placed in a 1% aqueous solution of tannic acid for 2hr and conductive-stained with 1% OsO4 for 2hr.

scholarly journals -254-ELECTRON MICROSCOPIC STUDIES ON IMPAIRED SARCOLEMMAL PERMEABILITY AND IRREVERSIBILITY OF ISCHEMIC MYOCARDIAL CELLS BY LANTHANUM PROBE METHOD

1986 ◽  
Vol 50 (6) ◽  
pp. 534
Author(s):  
Fumihiro TANNO ◽  
Kazuhide AKIYAMA ◽  
Tooru KITSU ◽  
Juichi HIROSHIGE ◽  
Shigeo HASEGAWA ◽  
...  
Keyword(s):  
Probe Method ◽  
Myocardial Cells ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
Sarcolemmal Permeability

Myocardial Alterations in Cats Kept at 14,110 Feet Elevation for Three Months

1969 ◽  
Vol 27 ◽  
pp. 364-365
Author(s):  
M. B. Bischoff ◽  
W. D. Dean ◽  
T. J. Bucci
Keyword(s):  
Electron Microscopy ◽  
High Altitude ◽  
Ventricular Hypertrophy ◽  
Animal Species ◽  
Cardiac Tissue ◽  
Light And Electron Microscopy ◽  
Myocardial Cells ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
Adult Cats

Right ventricular hypertrophy occurs in several animal species when transported from sea level to high altitude. Previous electron microscopic studies in our laboratory have shown enlargement of mitochondria with degeneration of cristae, separation of myofibrils, and increased accumulations of lipid and particulate glycogen in myocardial cells of dogs, rabbits and rats maintained at high altitude for five months. Edematous endothelial cells of the myocardial capillaries were also observed. Our recent work showed that adult cats native to 5,380 feet subjected to 14, 110 feet elevation for 90 days also developed cardiac hypertrophy. The cardiac tissue was examined by light and electron microscopy and the results comprise this communication.

Electron microscopic studies on ultrathin frozen sections of lactating mouse mammary gland

1978 ◽  
Vol 36 (2) ◽  
pp. 46-47
Author(s):  
Jan Zarzycki ◽  
Joseph Szroeder
Keyword(s):  
Mammary Gland ◽  
Protein Denaturation ◽  
Chemical Constituents ◽  
Freeze Substitution ◽  
Electron Microscopic Examination ◽  
Mouse Mammary Gland ◽  
Electron Microscopic ◽  
Preparation Methods ◽  
Microscopic Studies ◽  
Ultrathin Frozen Sections

The mammary gland ultrastructure in various functional states is the object of our investigations. The material prepared for electron microscopic examination by the conventional chemical methods has several limitations, the most important are the protein denaturation processes and the loss of large amounts of chemical constituents from the cells. In relevance to this,one can't be sure about a degree the observed images are adequate to the realy ultrastructure of a living cell. To avoid the disadvantages of the chemical preparation methods,some autors worked out alternative physical methods based on tissue freezing / freeze-drying, freeze-substitution, freeze-eatching techniqs/; actually the technique of cryoultraraicrotomy,i,e.cutting ultrathin sections from deep frozen specimens is assented as a complete alternative method. According to the limitations of the routine plastic embbeding methods we were interested to analize the mammary gland ultrastructure during lactation by the cryoultramicrotomy method.

Early Development of Drug-Induced Hepatic Necrosis

1971 ◽  
Vol 29 ◽  
pp. 576-577
Author(s):  
F. G. Zaki ◽  
E. Detzi ◽  
C. H. Keysser
Keyword(s):  
Early Development ◽  
Hepatic Necrosis ◽  
Screening Tests ◽  
Dense Matrix ◽  
Sprague Dawley ◽  
Electron Microscopic ◽  
Drug Induced ◽  
Hepatocellular Damage ◽  
Sprague Dawley Rats ◽  
Microscopic Studies

This study represents the first in a series of investigations carried out to elucidate the mechanism(s) of early hepatocellular damage induced by drugs and other related compounds. During screening tests of CNS-active compounds in rats, it has been found that daily oral administration of one of these compounds at a dose level of 40 mg. per kg. of body weight induced diffuse massive hepatic necrosis within 7 weeks in Charles River Sprague Dawley rats of both sexes. Partial hepatectomy enhanced the development of this peculiar type of necrosis (3 weeks instead of 7) while treatment with phenobarbital prior to the administration of the drug delayed the appearance of necrosis but did not reduce its severity.Electron microscopic studies revealed that early development of this liver injury (2 days after the administration of the drug) appeared in the form of small dark osmiophilic vesicles located around the bile canaliculi of all hepatocytes (Fig. 1). These structures differed from the regular microbodies or the pericanalicular multivesicular bodies. They first appeared regularly rounded with electron dense matrix bound with a single membrane. After one week on the drug, these vesicles appeared vacuolated and resembled autophagosomes which soon developed whorls of concentric lamellae or cisterns characteristic of lysosomes (Fig. 2). These lysosomes were found, later on, scattered all over the hepatocytes.

Electron Microscopic identification of Pre-B Lymphocytes in the Bone Marrow of a Patient with Chronic Myelocytic Leukemic in Blast Crisis

1982 ◽  
Vol 40 ◽  
pp. 346-347
Author(s):  
T. Mullin ◽  
G. Yee ◽  
M. Aheam ◽  
J. Trujillo
Keyword(s):  
Blast Crisis ◽  
Immunoglobulin M ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Transformation Theory ◽  
Electron Microscopic ◽  
Chemotherapeutic Sensitivity ◽  
Microscopic Studies ◽  
Hematopoietic Precursor ◽  
Lymphoblastic Transformation ◽  
B Lineage

There have been numerous reports in the current literature suggesting that hematopoietic precursor cells in some human chronic myelocytic leukemias (CML) undergo lymphoblastic transformation at the time of the acute blast crisis (BC) stage. The primary evidence offered in support of this transformation theory--lymphoblastic appearing morphology, increased terminal deoxynucleotidyl transferase (TdT) activity, and chemotherapeutic sensitivity to vincristine and prednisone--has been indirect, however, since these features may occur in nonlymphoid cells. More direct support for the Pre-B lineage of these cells has recently been provided by immunofluorescent light microscopic studies demonstrating the presence of intracytoplasmic immunoglobulin M (IgM) in these CML-BC cells.

Origin of Hepatic Nuclear Inclusion Bodies

1973 ◽  
Vol 31 ◽  
pp. 426-427
Author(s):  
F. G. Zaki ◽  
J. A. Greenlee ◽  
C. H. Keysser
Keyword(s):  
Inclusion Bodies ◽  
Storage Disease ◽  
Glycogen Storage ◽  
Nutritional Deficiencies ◽  
Nuclear Inclusion ◽  
Electron Microscopic ◽  
Human Liver Cells ◽  
Microscopic Studies ◽  
Per Se ◽  
Experimental Liver

Nuclear inclusion bodies seen in human liver cells may appear in light microscopy as deposits of fat or glycogen resulting from various diseases such as diabetes, hepatitis, cholestasis or glycogen storage disease. These deposits have been also encountered in experimental liver injury and in our animals subjected to nutritional deficiencies, drug intoxication and hepatocarcinogens. Sometimes these deposits fail to demonstrate the presence of fat or glycogen and show PAS negative reaction. Such deposits are considered as viral products.Electron microscopic studies of these nuclei revealed that such inclusion bodies were not products of the nucleus per se but were mere segments of endoplasmic reticulum trapped inside invaginating nuclei (Fig. 1-3).

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