Caries-Preventive Effect of NaF, NaF plus TCP, NaF plus CPP-ACP, and SDF Varnishes on Sound Dentin and Artificial Dentin Caries in vitro

2018 ◽  
Vol 52 (3) ◽  
pp. 199-211 ◽  
Author(s):  
Richard J. Wierichs ◽  
Sabrina Stausberg ◽  
Julian Lausch ◽  
Hendrik Meyer-Lueckel ◽  
Marcella Esteves-Oliveira
Keyword(s):  
Preventive Effect ◽  
Silver Diamine Fluoride ◽  
Photographic Images ◽  
Dentin Caries ◽  
High Caries Risk ◽  
Risk Patients ◽  
Ph Cycling ◽  
Casein Phosphopeptide ◽  
Post Hoc

The aim of this study was to compare the caries-preventive effect of different fluoride varnishes on sound dentin as well as on artificial dentin caries-like lesions. Bovine dentin specimens (n = 220) with one sound surface (ST) and one artificial caries lesion (DT) were prepared and randomly allocated to 11 groups. The interventions before pH cycling were as follows: application of a varnish containing NaF (22,600 ppm F-; Duraphat [NaF0/NaF1]), NaF plus tricalcium phosphate (22,600 ppm F-; Clinpro White Varnish Mint [TCP0/TCP1]), NaF plus casein phosphopeptide-stabilized amorphous calcium phosphate complexes (CPP-ACP; 22,600 ppm F-; MI Varnish [CPP0/CPP1]), or silver diamine fluoride (SDF; 35,400 ppm F-; Cariestop 30% [SDF0/SDF1]) and no intervention (NNB/N0/N1). During pH cycling (14 days, 6 × 120 min demineralization/day) half of the specimens in each group were brushed (10 s; 2 times/day) with either fluoride-free (“0”; e.g., TCP0) or 1,100 ppm F- (“1”; e.g., TCP1) dentifrice slurry. In another subgroup, the specimens were pH cycled but not brushed (NNB). Differences in integrated mineral loss (ΔΔZ), lesion depth (ΔLD), and colorimetric values (ΔΔE) were calculated between the values after initial demineralization and those after pH cycling, using transversal microradiography and photographic images. After pH cycling, no discoloration could be observed. Furthermore, NNB, N0, and N1 showed significantly increased ΔZDT/LDDT and ΔZST/LDST values, indicating further demineralization. In contrast, CPP0, CPP1, SDF0, and SDF1 showed significantly decreased ΔZDT/LDDT values, indicating remineralization (p ≤ 0.004; paired t test). CPP0, CPP1, SDF0, and SDF1 showed significantly higher changes in ΔΔZDT/ΔLDDT and ΔΔZST/ΔLDST than NNB, N0, and N1 (p < 0.001; Bonferroni post hoc test). In conclusion, under the conditions chosen, all fluoride varnishes prevented further demineralization. However, only NaF plus CPP-ACP and SDF could remineralize artificial dentin caries-like lesions under net-demineralizing conditions, thereby indicating that NaF plus CPP-ACP and SDF may be helpful to high-caries-risk patients.

scholarly journals Protective effect of titanium tetrafluoride and silver diamine fluoride on radiation-induced dentin caries in vitro

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Beatriz Martines de Souza ◽  
Mayara Souza Silva ◽  
Aline Silva Braga ◽  
Patrícia Sanches Kerges Bueno ◽  
Paulo Sergio da Silva Santos ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Protective Effect ◽  
Acid Production ◽  
Lactic Acid Production ◽  
Negative Control ◽  
Titanium Tetrafluoride ◽  
Silver Diamine Fluoride ◽  
Dentin Caries ◽  
Radiation Induced

AbstractThis in vitro study evaluated the protective effect of titanium tetrafluoride (TiF4) varnish and silver diamine fluoride (SDF) solution on the radiation-induced dentin caries. Bovine root dentin samples were irradiated (70 Gy) and treated as follows: (6 h): 4% TiF4 varnish; 5.42% NaF varnish; 30% SDF solution; placebo varnish; or untreated (negative control). Microcosm biofilm was produced from human dental biofilm (from patients with head-neck cancer) mixed with McBain saliva for the first 8 h. After 16 h and from day 2 to day 5, McBain saliva (0.2% sucrose) was replaced daily (37 °C, 5% CO2) (biological triplicate). Demineralization was quantified by transverse microradiography (TMR), while biofilm was analyzed by using viability, colony-forming units (CFU) counting and lactic acid production assays. The data were statistically analyzed by ANOVA (p < 0.05). TiF4 and SDF were able to reduce mineral loss compared to placebo and the negative control. TiF4 and SDF significantly reduced the biofilm viability compared to negative control. TiF4 significantly reduced the CFU count of total microorganism, while only SDF affected total streptococci and mutans streptococci counts. The varnishes induced a reduction in lactic acid production compared to the negative control. TiF4 and SDF may be good alternatives to control the development of radiation-induced dentin caries.

Demineralization Inhibitory Effects of Highly Concentrated Fluoride Dentifrice and Fluoride Gels/Solutions on Sound Dentin and Artificial Dentin Caries Lesions in vitro

2020 ◽  
pp. 1-14
Author(s):  
Daniel Erdwey ◽  
Hendrik Meyer-Lueckel ◽  
Marcella Esteves-Oliveira ◽  
Christian Apel ◽  
Richard Johannes Wierichs
Keyword(s):  
In Vitro Study ◽  
Inhibitory Effect ◽  
Negative Effects ◽  
Dentin Caries ◽  
Before And After ◽  
Amine Fluoride ◽  
Ph Cycling ◽  
Fluoride Dentifrice ◽  
Caries Lesions

<b><i>Objectives:</i></b> The aim of this in vitro study was to compare the demineralization inhibitory effect of gels/solutions used in combination with either standard or highly fluoridated dentifrices on sound dentin as well as on artificial dentin caries-like lesions. <b><i>Methods:</i></b> Bovine dentin specimens (<i>n</i> = 240) with two different surfaces each (sound [ST] and artificial caries lesion [DT]) were prepared and randomly allocated to twelve groups. Weekly interventions during pH-cycling (28 days, 6 × 120 min demineralization/day) were: the application of gels/solutions containing amine fluoride/sodium fluoride (12,500 ppm F [ppm]; pH = 4.4; AmF); NaF (12,500 ppm; pH = 6.6; NaF1); NaF (12,500 ppm; pH = 6.3; NaF2); silver diamine fluoride (14,200 ppm; pH = 8.7; SDF); acidulated phosphate fluoride (12,500 ppm; pH = 3.8; APF), and no intervention (standard control; S). Furthermore, half of the specimens in each group were brushed (10 s; twice per day) with dentifrice slurries containing either 1,450 ppm (e.g., AmF<sub>1450</sub>) or 5,000 ppm (e.g., AmF<sub>5000</sub>). Differences in integrated mineral loss (ΔΔZ) and lesion depth (ΔLD) were calculated between values before and after pH-cycling using transversal microradiography. <b><i>Results:</i></b> After pH-cycling Ss showed significantly increased ΔZ<sub>DT</sub> and LD<sub>DT</sub> values, indicating further demineralization. In contrast, except for one, all groups including fluoride gels/solutions showed significantly decreased ΔZ<sub>DT</sub> values. Additional use of most fluoride gels/solutions significantly enhanced mineral gain, mainly in the surface area; however, acidic gels/solutions seemed to have negative effects on lesion depths. <b><i>Significance:</i></b> Under the present pH-cycling conditions the highly fluoridated dentifrice significantly reduced caries progression and additional application of nearly all of the fluoride gels/solutions resulted in remineralization. However, there was no difference in the remineralizing capacity of fluoride gels/solutions when used in combination with either standard or highly fluoridated dentifrices.

scholarly journals In vitro Caries-Preventive Effect of Fluoridated Orthodontic Resins against Cariogenic Challenge Stimulation

2012 ◽  
Vol 13 (4) ◽  
pp. 452-455 ◽  
Author(s):  
Bhushan Arun Jawale ◽  
Neeraj Patil ◽  
Rahul Redasani ◽  
Lalit Chaudhari ◽  
JB Garde ◽  
...  
Keyword(s):  
Artificial Saliva ◽  
White Spot ◽  
White Spot Lesion ◽  
White Spot Lesions ◽  
Preventive Effect ◽  
Bonding Agents ◽  
Ph Cycling ◽  
Preventive Methods ◽  
Caries Lesions

ABSTRACT Aim The aim of the present study was to evaluate the in vitro caries preventive effect of fluoridated orthodontic resins under pH cycling with two types of acid demineralizing saliva. Materials and methods Brackets were bonded to 120 extracted human premolars, using Rely-a-bond (n = 40), Tru- Bond (n = 40) and Ortho-one (n = 40) orthodontic bonding agents. Each group of resin was divided into 2 subgroups (n = 20): immersion in remineralizing artificial saliva for 14 days and acid saliva with pH 4.3. After 14 days of pH cycling the caries preventive effect on the development of white spot lesion was evaluated considering the presence of inhibition zones to white spot lesions using two scores: 0 = absence and 1 = presence. Kruskal-Wallis ANOVA and Mann-Whitney U tests were used. Results Formation of white spot lesions was observed only under pH cycling using acid saliva with pH 4.3; with Rely-a-bond and Tru-Bond being significantly more effective in preventing the appearance of white spot lesions effect than Ortho-one. Conclusion The acidity of the demineralizing solution influenced the formation of white spot lesions around orthodontic brackets under highly cariogenic conditions. Rely-a-bond and Tru-bond presented higher caries-preventive effect than Orthoone. Clinical significance The development of fluoride-containing materials cannot be regarded as a permanent means to control dental caries lesions, but a complement along with other preventive methods. How to cite this article Patil N, Jawale B, Redasani R, Chaudhari L, Garde JB, Chauhan VS. In vitro Caries-Preventive Effect of Fluoridated Orthodontic Resins against Cariogenic Challenge Stimulation. J Contemp Dent Pract 2012;13(4):452-455.

scholarly journals The effect of silver diamine fluoride in preventing in vitro primary coronal caries under pH-cycling conditions

2021 ◽  
Vol 121 ◽  
pp. 104950
Author(s):  
Parand Sorkhdini ◽  
Yasmi O. Crystal ◽  
Qing Tang ◽  
Frank Lippert
Keyword(s):  
Silver Diamine Fluoride ◽  
Ph Cycling

Silver diamine fluoride remineralized artificial incipient caries in permanent teeth after bacterial pH-cycling in-vitro

2018 ◽  
Vol 69 ◽  
pp. 55-59 ◽  
Author(s):  
Kulnipa Punyanirun ◽  
Thanida Yospiboonwong ◽  
Thansinee Kunapinun ◽  
Panida Thanyasrisung ◽  
Chutima Trairatvorakul
Keyword(s):  
Permanent Teeth ◽  
Silver Diamine Fluoride ◽  
Ph Cycling ◽  
Incipient Caries

scholarly journals Preventive effect of fluoridated orthodontic resins subjected to high cariogenic challenges

2010 ◽  
Vol 21 (3) ◽  
pp. 211-215 ◽  
Author(s):  
Paula Passalini ◽  
Tatiana Kelly da Silva Fidalgo ◽  
Erika Machado Caldeira ◽  
Rogerio Gleiser ◽  
Matilde da Cunha Gonçalves Nojima ◽  
...  
Keyword(s):  
Color Change ◽  
Artificial Saliva ◽  
Orthodontic Brackets ◽  
White Spot ◽  
White Spot Lesion ◽  
White Spot Lesions ◽  
Preventive Effect ◽  
Ph Cycling ◽  
Inhibition Zones

The aim of the present study was to evaluate the in vitro caries preventive effect of fluoridated orthodontic resins under pH cycling with two types of acid demineralizing saliva. Brackets were bonded to 60 bovine incisors, using either Transbond Plus Color Change (n=30) or Orthodontic Fill Magic (n=30) orthodontic resins. Each group of resin was divided into 3 subgroups (n=10): immersion in remineralizing artificial saliva for 14 days, pH cycling with high cariogenic challenge in acid saliva with pH 5.5, and acid saliva with pH 4.5. After 14 days of pH cycling, the caries preventive effect on the development of white spot lesion was evaluated considering the presence of inhibition zones to white spot lesions using two scores: 0= absence and 1= presence. Kruskal Wallis and Mann-Whitney tests (a=0.05) were used. Formation of white spot lesions was observed only under pH cycling using acid saliva with pH 4.5; with Transbond Plus Color Change being significantly more effective (p<0.05) in preventing the appearance of white spot lesions effect than Orthodontic Fill Magic. The acidity of the demineralizing solution influenced the formation of white spot lesions around orthodontic brackets under highly cariogenic conditions. Transbond Plus Color Change resin presented higher caries preventive effect than Orthodontic Fill Magic.

scholarly journals Effect of Galla Chinensis on Remineralization of Early Dentin Lesion

2020 ◽  
Vol 14 (04) ◽  
pp. 651-656
Author(s):  
Hend Mahmoud Abdel-Azem ◽  
Ahmed Fawzy Abo Elezz ◽  
Rehab Khalil Safy
Keyword(s):  
Treatment Time ◽  
Analysis Data ◽  
Environmental Scanning ◽  
X Ray ◽  
Parametric Data ◽  
Significant Difference ◽  
Ph Cycling ◽  
The Mean ◽  
Post Hoc

Abstract Objective This study aimed to investigate the efficacy of the Galla chinensis extract (GCE) on early dentin lesion remineralization. Materials and Methods Seventy-two dentin specimens were divided into three groups according to the treatment solution. In group S1, specimens were treated with GCE; in group S2, the specimens were treated with sodium fluoride (NaF); meanwhile, specimens of group S3 were treated with distilled water (DW). Each group was further subdivided into two subgroups according to the treatment time (1 minute and 5 minutes). An in vitro pH-cycling model for 12 days was done. Subsequently, surface microhardness (SMH) of the specimens, elemental analysis, and their micromorphological appearance were evaluated. Statistical Analysis Data were statistically analyzed. One-way analysis of variance was used to compare numerical (parametric) data between more than two separate groups followed by post hoc Tukey. Results There was no significant difference between the mean SMH of dentin between NaF and GCE groups. Regarding the time, the 5-minute treatment with NaF and DW groups recorded higher mean SMH value of dentin than the 1-minute treatment group. Meanwhile, for GCE groups, the 1-minute immersion recorded higher mean SMH value than the 5-minute immersion without any significant difference between them. The microhardness results were confirmed by environmental scanning electron microscope and energy dispersive X-ray analysis results. Conclusion GCE could be used as an effective alternative for dentin remineralization.

scholarly journals Preventing and arresting primary tooth enamel lesions using self-assembling peptide P11-4 in vitro

2021 ◽  
Author(s):  
Nour Wahba ◽  
Falk Schwendicke ◽  
Mohamed Amr Kamel ◽  
Gehan Allam ◽  
Sebastian Paris ◽  
...  
Keyword(s):  
Tooth Enamel ◽  
Minimal Invasive ◽  
Primary Tooth ◽  
Treatment Needs ◽  
Invasive Treatment ◽  
Self Assembling ◽  
Ph Cycling ◽  
Casein Phosphopeptide ◽  
Negative Controls

Abstract Background: Self-assembling peptides (SAP) may prevent and/or arrest caries lesions. The aim of the study was to evaluate SAP for caries prevention and arrest in primary tooth enamel in vitro. Methods: 215 extracted primary teeth were used. In the prevention experiment, self-assembling peptide for prevention (SAPP), fluoride varnish/mouthwash (FV/FMW), casein-phosphopeptide amorphous-calcium phosphate (CPP-ACP), nanohydroxy-apatite (nHA) were applied. Samples were subjected to a demineralizing pH-cycling for 14-days. In the arrest experiment, 60 samples were pre-demineralized and induced lesions treated using self-assembling peptide for repair (SAPR), FV, CPP-ACP plus fluoride and resin infiltration (RI), and submitted to pH-cycling. Thirty-five samples were used as negative controls (NC). Mineral loss and its differences (Δ/ΔΔZ) were determined using transversal microradiography. Results: FV (ΔZ median: -46 [interquartile range: 189] vol%×µm) and FMW (-28 [129] vol%×µm) prevented caries significantly more effective than all other groups (p<0.05), which did not show significant preventive effects compared with NC (ΔZ=1446 [378] vol%×µm). RI (ΔΔZ=1808 [2193] vol%×µm) and FV (ΔΔZ=1494 [4274] vol%×µm) arrested lesions compared with NC (ΔΔZ=5605 [1371] vol%×µm; p<0.05), while SAPR and CPP-ACPF did not show such arrest. Conclusions: FV and FMW showed the largest caries-preventive effect, while RI and FV arrested lesion progression in primary tooth enamel in vitro. Clinical significance: Preventing and arresting caries lesion allows decreasing invasive treatment needs and patient’s morbidity and fosters the application of minimal invasive dentistry approach.

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