Reduced Protein C Global Assay Levels in Infertile Women with in vitro Fertilization Failure: A Pilot Study

2018 ◽  
Vol 139 (1) ◽  
pp. 1-6 ◽  
Author(s):  
Sally El Masry ◽  
Hanan Azzam ◽  
Hamed Youssef ◽  
Maha Othman ◽  
Mohamed Awad
Keyword(s):  
In Vitro Fertilization ◽  
Protein C ◽  
Activated Protein C ◽  
Control Group ◽  
Vitro Fertilization ◽  
Infertile Women ◽  
Ivf Failure ◽  
History Of

Protein C global is a global dotting assay that evaluates abnormalities in the protein C anticoagulant pathway. A few studies have examined this assay in relation to assisted reproductive technology (ART), but its role in infertile women with in vitro fertilization (IVF) failure remains unclear. In this study, we assessed protein C in infertile women with a history of IVF failure who were undergoing ART. We examined 45 healthy fertile women who conceived naturally, and 45 infertile women with 2 or more implantation failures undergoing ART. Both protein C and activated protein C resistance (APC-R) were evaluated. The results showed that mean protein C expressed as a normalized ratio (PCAT-NR) was significantly lower in the study group compared to the control group (0.76 ± 0.15 vs. 0.91 ± 0.14, respectively; p = 0.0001). Follow-up on ART outcomes showed that women who failed ART had significantly lower PCAT-NR compared to successful cases. PCAT-NR did not correlate with APC-R levels in the study (r = 0.125, p < 0.5) or failed ART subgroups. Using logistic regression analysis, patients with lower PCAT-NR levels showed an elevated risk of implantation failure (p = 0.04, OR 0.50, 95% CI 0.26-0.84). In conclusion, protein C global assay may play a role in the etiology of IVF failure, which might be independent of APC-R. Larger studies are encouraged to validate these findings and explore the underlying pathophysiological mechanisms.

scholarly journals The effectiveness of collaborative infertility counseling (CIC) on pregnancy outcome in infertile women undergoing in vitro fertilization: A randomized controlled trial

2019 ◽  
Author(s):  
Mahboobeh Rasoulzadeh Bidgoli ◽  
robab latifnejad roudsari ◽  
ali montazeri
Keyword(s):  
In Vitro Fertilization ◽  
Pregnancy Rate ◽  
Treatment Success ◽  
Intervention Group ◽  
Control Group ◽  
Vitro Fertilization ◽  
Infertile Women ◽  
Significant Difference ◽  
And Control

Abstract Background: Infertility is an emotional tension which influences the whole aspects of relationships in infertile couples. A main objective of infertility treatments is elevation of pregnancy rate. The present study aimed to examine the effect of collaborative counseling on pregnancy rate in infertile women, undergoing in vitro fertilization in Mashhad, Iran. Methods: In this clinical trial, 60 women with primary infertility were selected from an infertility research center and were randomly allocated into intervention (n=29) and control (n=31) groups. The intervention group received individual counseling, based on the collaborative reproductive healthcare model with collaboration of a midwife, a gynecologist and a clinical psychologist in five sessions during a two-month period. The control group received routine care. Positive pregnancy test was considered as a criterion of treatment success at the end of the study. Data were analyzed using statistical tests including independent samples t-test. Results: There was no significant difference in pregnancy rate between intervention and control groups (P = 0.298). Also, there were no significant differences in follicle and embryo numbers between two groups. However, a significant difference was observed between two groups in terms of oocyte numbers where the intervention group had more oocyte (P = 0.014). Conclusion: Overall the findings indicated that the collaborative infertility counseling did not improve treatment success in infertile women undergoing in vitro fertilization

Activated protein C resistance during in vitro fertilization treatment

2001 ◽  
Vol 95 (2) ◽  
pp. 222-224 ◽  
Author(s):  
J. Curvers ◽  
S.J. Nienhuis ◽  
A.W. Nap ◽  
K. Hamulyák ◽  
J.L.H. Evers ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Protein C ◽  
Activated Protein C ◽  
Activated Protein C Resistance ◽  
Vitro Fertilization

scholarly journals Effect of intrauterine granulocyte-colony stimulating factor administration on in vitro fertilization outcome in women with moderate-to-severe endometriosis: An RCT

2021 ◽  
pp. 733-740
Author(s):  
Ladan Kashani ◽  
Ashraf Moini ◽  
Tayebeh Esfidani ◽  
Nazila Yamini ◽  
Shima Mohiti
Keyword(s):  
In Vitro Fertilization ◽  
Clinical Pregnancy ◽  
Control Group ◽  
Granulocyte Colony Stimulating Factor ◽  
Colony Stimulating Factor ◽  
Vitro Fertilization ◽  
Infertile Women ◽  
Significant Difference ◽  
Stimulating Factor

Background: Nearly 25-50% of infertile women have endometriosis. There are reports of disorders in the expression of granulocyte colony-stimulating factor (G-CSF) receptors in women with endometriosis. Objective: To examine the effect of intrauterine administration of G-CSF in in vitro fertilization (IVF) cycles on the fertility rate of infertile women with moderate-to-severe endometriosis. Materials and Methods: This clinical trial was conducted on 66 infertile women with moderate-to-severe endometriosis, undergoing IVF and intracytoplasmic sperm injection (ICSI). The participants were allocated into two groups via simple randomization: the G-CSF (n = 27) and control (n = 39) groups. In the G-CSF intervention group, on the oocyte pick-up day, immediately after an ovarian puncture, 300 μg of G-CSF was injected through a transcervical catheter under abdominal ultrasound guide to visualize flushing into the uterine cavity. Women in the control group received no intervention. The two groups were evaluated for clinical pregnancy. Results: No significant difference was noted in the demographic characteristics of the two groups. The rate of clinical pregnancy was 28.2% in the control group and 25.9% in the G-CSF group, indicating no significant difference (p = 0.83). Conclusion: The results showed that the intrauterine injection of G-CSF had no effects on pregnancy in women with stage-3/4 endometriosis undergoing IVF. Key words: G-CSF, In vitro fertilization, Endometriosis, Pregnancy.

scholarly journals Association Between Systemic and Local Oxidative Stress of Infertile Women Undergoing Ivf/Icsi

2019 ◽  
pp. 1888-1897
Author(s):  
Qais Ahmed Mahdi ◽  
Shatha Abdul Wadood ◽  
Rusul H Hamza
Keyword(s):  
Oxidative Stress ◽  
In Vitro Fertilization ◽  
Polycystic Ovary ◽  
Stress Index ◽  
Control Group ◽  
Vitro Fertilization ◽  
Infertile Women ◽  
Pcos Group ◽  
Gst Activity

     Oxidative stress (OS) may affect in vitro fertilization (IVF) outcomes in infertile women undergoing in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI). The aim of this study is to explore the possible relationships between OS parameters in serum and follicular fluid (FF) from infertile women with male cause infertility (n=40), women with polycystic ovary syndrome (PCOS) (n=40), and women with unexplained infertility (UI)(n=45) undergoing IVF/ICSI. The collection of blood and FF samples was done at the day of oocyte aspiration. Total peroxide (TPX) level, total antioxidant capacity (TAC), and malondialdehyde (MDA) level were measured in serum and FF; whereas, glutathione-s-transferase (GST) activity and superoxide dismutase (SOD) activity were measured in FF. Also, oxidative stress index (OSI) that is the percentage ratio of TPX to TAC, was calculated. In the control group, correlation analysis reveals the presence of a significant positive association between FF OSI with serum OSI, FF TPX with FF OSI, and serum TPX with FF GST activity. In the PCOS group, there was a significant negative association between: FF TPX and serum TAC level. However, non-significant relationship was found between serum and FF OS status parameters in the UI group. It’s concluded from the present study that systemic OS may give valuable information about local OS occurrence (blood OS reflects FF OS) only in control group and PCOS group. Such information could be useful for a better understanding of the pathological OS mechanisms involved in IVF failure for patients with different causes of infertility.

scholarly journals The clinical outcome of Dienogest treatment followed by in vitro fertilization and embryo transfer in infertile women with endometriosis

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Hiroshi Tamura ◽  
Hiroaki Yoshida ◽  
Hiroyuki Kikuchi ◽  
Mai Josaki ◽  
Yumiko Mihara ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
In Vitro Fertilization ◽  
Clinical Outcome ◽  
Control Group ◽  
Vitro Fertilization ◽  
Infertile Women ◽  
Significant Difference ◽  
Ivf Et ◽  
Follicular Fluids

Abstract Background Endometriosis is considered to be the most intractable cause of female infertility. Administering any type of treatment for endometriosis before in vitro fertilization and embryo transfer (IVF-ET) is an important strategy for improving the IVF-ET outcomes for infertile women with endometriosis. In fact, treatment with a gonadotropin-releasing hormone (GnRH) agonist just before IVF-ET has been reported to improve the clinical outcome in endometriosis patients. However, the benefit of Dienogest (DNG), a synthetic progestin, treatment just before IVF-ET remains unclear. Methods Sixty-eight infertile women with Stage III or IV endometriosis (ovarian endometrial cyst < 4 cm) were recruited for this study. The subjects were divided into 2 groups: a DNG group (n = 33) and a control group (n = 35). DNG was administered orally every day for 12 weeks prior to the conventional IVF-ET cycle in the DNG group. Standard controlled ovarian hyperstimulation with the GnRH agonist long protocol was performed in the control group. The numbers of mature follicles and retrieved oocytes, fertilization rates, implantation rates, and clinical pregnancy rate were compared between the two groups. In addition, the concentrations of inflammatory cytokines, oxidative stress markers, and antioxidants in follicular fluids were also measured. Results The numbers of growing follicles, retrieved oocytes, fertilized oocytes, and blastocysts were significantly lower in the DNG group than in the control group. The fertilization and blastocyst rates were also lower in the DNG group than in the control group. Although there was no significant difference in the implantation rate between the groups, the cumulative pregnancy rate and live birth rate were lower in the DNG group than in the control group. There was no significant difference in the abortion rate. Our results failed to show that DNG reduces the inflammatory cytokine levels and oxidative stress in follicular fluids. Conclusions Administering DNG treatment just before IVF-ET did not provide any benefits to improve the clinical outcomes for infertile women with endometriosis.

scholarly journals Analysis of in vitro fertilization/intracytoplasmic sperm injection outcomes in infertile women with a history of thyroid cancer: a retrospective study

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Ning Huang ◽  
Lin Zeng ◽  
Jie Yan ◽  
Hongbin Chi ◽  
Jie Qiao
Keyword(s):  
Thyroid Cancer ◽  
In Vitro Fertilization ◽  
Preterm Delivery ◽  
Intracytoplasmic Sperm Injection ◽  
Obstetric Outcomes ◽  
Control Group ◽  
High Grade ◽  
Vitro Fertilization ◽  
History Of

Abstract Background Recent studies have revealed that women with infertility have a higher risk of thyroid cancer (TC) than fertile women. However, studies on whether a history of thyroid cancer affects clinical outcomes in women who conceive using in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) are scarce. We investigate whether a history of thyroid cancer (TC) affects the in vitro fertilization/intracytoplasmic sperm injection (IVF/ICSI) outcomes and increases the risk of adverse obstetric outcomes in women with infertility. Methods This retrospective study enrolled 384 women with infertility who underwent their first IVF/ICSI treatment at the Peking University Third Hospital between 2010 and 2019. Participants were divided into the TC (64 women with TC history) and control (320 women matched from 85,272 women without thyroid diseases) groups. Controls were individually matched to the TC group according to age, body mass index, concomitant infertility factors, first IVF/ICSI dates, and controlled ovarian stimulation and embryo transfer procedure protocols. IVF/ICSI outcomes, including the numbers of retrieved oocytes and high-grade embryos, clinical pregnancy, miscarriage, preterm delivery, and live birth rates, and adverse obstetric outcome risk were assessed. Results The TC group had significantly higher thyroid hormone and lower thyroid-stimulating hormone (TSH) levels than the control group. Despite similar gonadotropin treatment dosage, the TC group had a significantly lower numbers of retrieved oocytes and high-grade embryos than the control group. The occurrence rates of clinical pregnancy, miscarriage, preterm delivery, live births, and adverse obstetric outcomes, including multiple gestation, preterm delivery, gestational diabetes mellitus, gestational hypertension, low birth weight, and large-for-gestational-age infants, were not significantly different between the two groups. Conclusions TC history did not affect the pregnancy outcomes or increase the risk of adverse obstetric outcomes after the first IVF/ICSI, but it may decrease the number of retrieved oocytes and high-grade embryos.

scholarly journals Stress in women with Reproductive Failure

2016 ◽  
Vol 11 (1) ◽  
pp. 14-19 ◽  
Author(s):  
Sunita Bhandari
Keyword(s):  
In Vitro Fertilization ◽  
Nk Cells ◽  
Health Survey ◽  
Recurrent Miscarriage ◽  
Reproductive Failure ◽  
Serum Cortisol Level ◽  
Control Group ◽  
Vitro Fertilization ◽  
Infertile Women

Aims: The study aim to measure the level of stress in women with recurrent miscarriage and infertile women undergoing in-vitro fertilization treatment using psychological, endocrinological and immunological markers of stress.Methods: Group A（Recurrent Miscarriage Group, RM Group） of 20 women who had had at least three or more consecutive spontaneous abortions after negative biomedical diagnostic screening test; Group B (Infertility Group) of 40 infertile women undergoing in-vitro fertilization treatment after down-regulation with Gonadotropin-releasing Hormone (GnRH) agonist in a long protocol and Group C (Control Group) of 15 fertile women were asked to complete the stress questionnaires : the Perceived stress scale (PSS), the Fertility Problem Inventory (FPI), the Positive and Negative Scale (PANAS) and the SF-12 Health Survey. Blood samples were collected to measure natural killer (NK) cells and cortisol levels.Results: The score of stress questionnaires were significantly high in women with reproductive failure compared to fertile control group (p=0.023 for PSS; p=0.011 for FPI; p=0.013 for negative scale of PANAS) and the score of MCS from SF-12 Health Survey was significantly lower compared to control group (p=0.001). However, no significant differences were seen between women with RM and infertile women undergoing IVF treatment. Women with reproductive failure have increased number of peripheral NK cells compared to controls (p=0.000). No significant difference was found in the serum cortisol level between the women with reproductive failure and fertile women.Conclusions: Women with reproductive failure perceived higher levels of psychological stress. Level of stress in women with recurrent miscarriage is comparable to that of infertile women undergoing in-vitro fertilization treatment. Increased numbers of NK cells were found in the peripheral blood of women with reproductive failure.

scholarly journals T Cell Subsets Profiling in Unexplained Infertile Women with Successful and Unsuccessful in vitro Fertilization Outcome: Focus on the Effect of Seminal Plasma

2019 ◽  
Author(s):  
Zahra Kanannejad ◽  
Bahia Namavar Jahromi ◽  
Behrouz Gharesi-Fard
Keyword(s):  
In Vitro Fertilization ◽  
Seminal Plasma ◽  
T Cell Subsets ◽  
Control Group ◽  
Ivf Outcome ◽  
Color Flow ◽  
Vitro Fertilization ◽  
Infertile Women ◽  
Th Cell

Unexplained infertility (UI) is one of the most common diagnoses in the fertility care. Seminal plasma (SP) plays a crucial role in the regulation of female immune responses and the success of a pregnancy. In vitro fertilization (IVF) is a well-known method for the treatment of UI. In this study, we aimed to investigate the effect of SP on the differentiation of T helper (Th) cell subsets and the relationship between these subsets with the rate of IVF success in a group of women complicated with UI compared to those with normal pregnancy. This study was conducted on 20 UI couples (ten with successful and ten with unsuccessful IVF outcome) and 10 fertile couples as the control group. Four color flow cytometry technique was used to detect Th cell subsets in the peripheral blood mononuclear cells (PBMC) with or without stimulation by SP. Results indicated that the frequencies of IL-17+ and Foxp3+ T cells after incubation with SP was significantly increased in couples with unsuccessful IVF outcome as compared to successful and healthy groups (p<0.05). Additionally, a positive correlation was observed between Th1 and Th2 cells in the unsuccessful IVF group (R=0.6, p=0.03). In summary, the results of the present study demonstrated that exposure to SP might increase Th17 and Treg cell frequencies in infertile women with unsuccessful IVF, and might also balance inflammatory to regulatory responses to finally tune-up the Th1/Th2/Th17/Treg balance and support the success of IVF.

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