Contribution of PCR Denaturing Gradient Gel Electrophoresis Combined with Mixed Chromatogram Software Separation for Complex Urinary Sample Analysis
2017 ◽
Vol 27
(6)
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pp. 350-355
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Keyword(s):
Complex samples are a challenge for sequencing-based broad-range diagnostics. We analysed 19 urinary catheter, ureteral Double-J catheter, and urine samples using 3 methodological approaches. Out of the total 84 operational taxonomic units, 37, 61, and 88% were identified by culture, PCR-DGGE-SS (PCR denaturing gradient gel electrophoresis followed by Sanger sequencing), and PCR-DGGE-RM (PCR- DGGE combined with software chromatogram separation by RipSeq Mixed tool), respectively. The latter approach was shown to be an efficient tool to complement culture in complex sample assessment.
2001 ◽
Vol 47
(9)
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pp. 829-841
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2002 ◽
Vol 14
(3)
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2008 ◽
Vol 99
(4)
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pp. 730-738
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2005 ◽
Vol 60
(2)
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pp. 143-154
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2011 ◽
Vol 2011
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pp. 1-8
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2003 ◽
Vol 69
(11)
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pp. 6380-6385
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1999 ◽
Vol 96
(6)
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pp. 2913-2918
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2003 ◽
Vol 61
(1)
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pp. 10-14
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