scholarly journals Effect of Glomerular Mannose-Binding Lectin Deposition on the Prognosis of Idiopathic Membranous Nephropathy

2020 ◽  
Vol 45 (5) ◽  
pp. 713-726
Author(s):  
Ying Zhang ◽  
Yipeng Liu ◽  
Liming Liang ◽  
Liyan Liu ◽  
Xueqing Tang ◽  
...  
Keyword(s):  
Membranous Nephropathy ◽  
Cox Proportional Hazards ◽  
Mannose Binding Lectin ◽  
Clinical Event ◽  
Idiopathic Membranous Nephropathy ◽  
Antibody Concentration ◽  
Phospholipase A2 Receptor ◽  
Significant Difference ◽  
Mannose Binding ◽  
Binding Lectin

Objective: Co-deposition of mannose-binding lectin (MBL) and IgG4 anti-phospholipase A2 receptor (anti-PLA2R) autoantibodies under subepithelial cells has been observed in patients with idiopathic membranous nephropathy (iMN), but the relationships of MBL deposition to iMN severity and progression remain unclear. Methods: Patients diagnosed with iMN who underwent renal puncture were enrolled and followed up for a median of 17 months (interquartile range [IQR], 9–25 months). Serum anti-PLA2R and anti-thrombospondin type-1 domain-containing 7A antibodies and MBL were detected by ELISA. Glomerular MBL and anti-PLA2R antibodies were detected by immunofluorescence. Proteinuria remission, including complete remission (CR), was defined as a clinical event. Clinicopathological characteristics and kidney outcomes were compared between patients with and without MBL deposition. Results: In 67 prevalent patients with biopsy-proven iMN, serum anti-PLA2R antibodies and anti-THSD7A antibodies were present in 37 (55.3%) and 1 (1.4%) patient with iMN. The positivity of glomerular MBL deposition and tissue anti-PLA2R antibody was 53 (79.1%) and 49 (73.1%), respectively. No significant difference was found between the MBL-positive and negative groups in the albumin level (26.5 ± 6.6 and 28.6 ± 6.1 g/L), eGFR (104.8 ± 17.4 and 114.6 ± 16.1 mL/min/1.73 m2), 24-h proteinuria (5.35 and 4.25 g/day), or serum MBL level corrected by serum Cr 4.92 (IQR, 0.86, 8.90) and 2.28 (IQR, 0.4, 5.62). In a Cox proportional hazards regression model adjusted for sex, age, systolic blood pressure, eGFR, immunosuppressive agent use, 24-h proteinuria, and anti-PLA2R antibody concentration, glomerular MBL deposition was independently associated with ICR of proteinuria (HR, 6.31; 95% CI, 1.1–36.1; p = 0.039). Conclusions: The MBL pathway of complement activation is commonly initiated in patients with iMN, and patients with MBL deposition reach ICR faster than patients without MBL deposition.

Glomerular deposition of L-ficolin and mannose-binding lectin in a patient with membranous nephropathy: Activation of the lectin complement pathway

2007 ◽  
Vol 44 (16) ◽  
pp. 3993
Author(s):  
Hiroyuki Inoshita ◽  
Isao Ohsawa ◽  
Kisara Onda ◽  
Satoshi Horikoshi ◽  
Hiroyuki Ohi ◽  
...  
Keyword(s):  
Membranous Nephropathy ◽  
Mannose Binding Lectin ◽  
Complement Pathway ◽  
Mannose Binding ◽  
Binding Lectin

scholarly journals Glomerular mannose-binding lectin deposition in intrinsic antigen-related membranous nephropathy

2017 ◽  
Vol 33 (5) ◽  
pp. 832-840 ◽  
Author(s):  
Norifumi Hayashi ◽  
Keiichirou Okada ◽  
Yuki Matsui ◽  
Keiji Fujimoto ◽  
Hiroki Adachi ◽  
...  
Keyword(s):  
Membranous Nephropathy ◽  
Mannose Binding Lectin ◽  
Mannose Binding ◽  
Binding Lectin

scholarly journals Association of Mannose-Binding Lectin Gene Polymorphism but Not of Mannose-Binding Serine Protease 2 with Chronic Severe Aortic Regurgitation of Rheumatic Etiology

2008 ◽  
Vol 15 (6) ◽  
pp. 932-936 ◽  
Author(s):  
Rajendranath Ramasawmy ◽  
Guilherme S. Spina ◽  
Kellen C. Fae ◽  
Alexandre C. Pereira ◽  
Renato Nisihara ◽  
...  
Keyword(s):  
Aortic Regurgitation ◽  
Cross Reactivity ◽  
Mannose Binding Lectin ◽  
Streptococcal Cell Wall ◽  
Severe Aortic Regurgitation ◽  
Significant Difference ◽  
Mannose Binding ◽  
Exon 1 ◽  
Valve Lesion ◽  
Binding Lectin

ABSTRACT N-Acetylglucosamine (GlcNAc) is the major immunoepitope of group A streptococcal cell wall carbohydrates. Antistreptococcal antibodies cross-reactive with anti-GlcNAc and laminin are present in sera of patients with rheumatic fever. The cross-reactivity of these antibodies with human heart valvular endothelium and the underlying basement membrane has been suggested to be a possible cause of immune-mediated valve lesion. Mannose-binding lectin (MBL) encoded by the MBL2 gene, a soluble pathogen recognition receptor, has high affinity for GlcNAc. We postulated that mutations in exon 1 of the MBL2 gene associated with a deficient serum level of MBL may contribute to chronic severe aortic regurgitation (AR) of rheumatic etiology. We studied 90 patients with severe chronic AR of rheumatic etiology and 281 healthy controls (HC) for the variants of the MBL2 gene at codons 52, 54, and 57 by using a PCR-restriction fragment length polymorphism-based method. We observed a significant difference in the prevalence of defective MBL2 alleles between patients with chronic severe AR and HC. Sixteen percent of patients with chronic severe AR were homozygotes or compound heterozygotes for defective MBL alleles in contrast to 5% for HC (P = 0.0022; odds ratio, 3.5 [95% confidence interval, 1.6 to 7.7]). No association was detected with the variant of the MASP2 gene. Our study suggests that MBL deficiency may contribute to the development of chronic severe AR of rheumatic etiology.

scholarly journals What is the Role of Mannose-Binding Lectin Gene Polymorphism in the Development of Acute Post-Streptococcal Glomerulonephritis?

2021 ◽  
Author(s):  
Deniz Güven ◽  
Mustafa Bak ◽  
Erkin Serdaroğlu ◽  
Ferda Özkınay ◽  
Ayça Aykut
Keyword(s):  
Gene Polymorphism ◽  
Mannose Binding Lectin ◽  
Response To Treatment ◽  
Control Group ◽  
Gene Frequencies ◽  
Laboratory Findings ◽  
Significant Difference ◽  
Mannose Binding ◽  
Post Streptococcal Glomerulonephritis ◽  
Binding Lectin

Objective: This study aims to determine the effects of the mannose-binding lectin (MBL) gene polymorphism on the clinical and laboratory findings, response to treatment, and progress of patients with acute post-streptococcal glomerulonephritis (APSGN). Methods: Codon 54 polymorphism found in exon 1 of the MBL gene was investigated by polymerase chain reaction-restriction fragment length polymorphism method in 110 children followed up with the diagnosis of APSGN and compared with healthy control group. Results: The normal allele AA and, the variant alleles AB and BB gene frequencies were determined within the APSGN group as 74.5%, 20% and, 5.5%, respectively. No statistically significant difference was found with concerning to the gene polymorphism in the APSGN group when compared with the control group (p>0.05). No correlation was found in the patient group between gene polymorphism and the presence of hematuria, edema, central nervous system findings, and blood pressure (p>0.05). Concerning laboratory findings during the diagnosis, no correlation existed between the gene polymorphism and high levels of urea, creatine, total cholesterol, and triglycerides, low levels of albumin, and the presence of proteinuria (p>0.05). Within the first years following the diagnosis, no statistically significant difference was found in the glomerular filtration rates, blood creatine levels, proteinuria levels, duration of microscopic hematuria and proteinuria between the patients with the gene polymorphism and those without the gene polymorphism (p>0.05) Conclusion: Our study determined that the MBL gene polymorphism was not important in the development, the laboratory and clinical findings, or the progression of the patients with APSGN.

scholarly journals Mannose-Binding Lectin Levels and Carotid Intima-Media Thickness in Type 2 Diabetic Patients

2016 ◽  
Vol 2016 ◽  
pp. 1-8 ◽  
Author(s):  
Miklós Káplár ◽  
Shah Sweni ◽  
Julianna Kulcsár ◽  
Barbara Cogoi ◽  
Regina Esze ◽  
...  
Keyword(s):  
Intima Media Thickness ◽  
Carotid Intima Media Thickness ◽  
Mannose Binding Lectin ◽  
Diabetic Patients ◽  
Media Thickness ◽  
Significant Difference ◽  
Mannose Binding ◽  
Binding Lectin ◽  
Type 2 Diabetic

Introduction. Mannose-binding lectin (MBL) activates complement system and has been suggested to play a role in vascular complications in diabetics. Carotid intima-media thickness (cIMT) detects subclinical atherosclerosis. We evaluated the association of MBL and IMT in type 2 diabetic (T2DM) patients.Methods. Serum MBL levels and cIMT were measured in a total of 103 diabetics and in 98 age-matched healthy controls.Results. There was no significant difference in MBL level in T2DM versus controls. As expected, IMT was significantly higher in T2DM patients than in controls (P=0.001). In T2DM, the lowest cIMT was seen in patients with normal MBL level (500–1000) while cIMT continuously increased with both high MBL and absolute MBL deficiency states. This was especially significant in high MBL versus normal MBL T2DM patients (P=0.002). According to multiple regression analysis the main predictors of IMT in T2DM are age (P<0.003), ApoA level (P=0.023), and the MBL (P=0.036).Conclusions. Our results suggest a dual role of MBL as a risk factor for cIMT in T2DM. MBL may also be used as a marker of macrovascular disease, as both low and high levels indicate the susceptibility for atherosclerosis in T2DM.

Serum Anti-PLA2R Antibody Predicts Treatment Outcome in Idiopathic Membranous Nephropathy

2016 ◽  
Vol 43 (2) ◽  
pp. 129-140 ◽  
Author(s):  
Shi-Yao Wei ◽  
Yu-Xiao Wang ◽  
Jian-Si Li ◽  
Shi-Lei Zhao ◽  
Tian-Tian Diao ◽  
...  
Keyword(s):  
Treatment Outcome ◽  
Membranous Nephropathy ◽  
Immunosuppressive Agents ◽  
Idiopathic Membranous Nephropathy ◽  
Enzyme Linked Immunosorbent Assay ◽  
Target Antigen ◽  
Phospholipase A2 Receptor ◽  
Significant Difference ◽  
Major Target

Background: M-type phospholipase A2 receptor (PLA2R) has been identified as the major target antigen in idiopathic membranous nephropathy (IMN). However, the role of glomerular PLA2R (gPLA2R) and the associations of serum anti-PLA2R antibody (sPLA2R-Ab) titre with diagnosis, treatment and prognosis in IMN need to be further investigated. Methods: We screened 148 consecutive patients with biopsy-proven membranous nephropathy (MN; 113 with IMN and 35 with secondary MN (SMN)) who were followed up for ≤20 months. Serum and urine samples were simultaneously collected at different time points. The levels of sPLA2R-Ab were detected using immunofluorescence and enzyme-linked immunosorbent assay. gPLA2R was assessed by immunofluorescence. Results: Most patients with IMN displayed both gPLA2R and sPLA2R-Ab positive (85.8 and 82.3%, respectively). In contrast, very few patients with SMN showed either gPLA2R or sPLA2R-Ab positive. The sPLA2R-Ab titre, not gPLA2R, was significantly correlated with proteinuria. Surprisingly, changes in sPLA2R-Ab titre occurred earlier and faster than proteinuria in patients who were followed up for ≤20 months during the whole period of observation. Survival analysis of IMN patients indicated a significant association between sPLA2R-Ab titre and outcome, whereas, no significant difference was observed between the gPLA2R intensity and outcome. Conclusions: These data indicate that sPLA2R-Ab might be a better biomarker for IMN diagnosis and treatment outcome. In addition, monitoring sPLA2R-Ab titre may assist in determining when to initiate the administration of immunosuppressive agents and in evaluating treatment efficacy.

Mannose-binding Lectin Gene Polymorphisms in Brazilian Patients with Rheumatoid Arthritis

2011 ◽  
Vol 39 (1) ◽  
pp. 6-9 ◽  
Author(s):  
FERNANDA LETICIA MARTINY ◽  
TIAGO DEGANI VEIT ◽  
CLAITON VIEGAS BRENOL ◽  
JOÃO CARLOS TAVARES BRENOL ◽  
RICARDO MACHADO XAVIER ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Gene Polymorphisms ◽  
Clinical Manifestations ◽  
Mannose Binding Lectin ◽  
Unknown Etiology ◽  
Significant Difference ◽  
Mannose Binding ◽  
Binding Lectin ◽  
Reaction Sequencing

Objective.Rheumatoid arthritis (RA) is a disease with unknown etiology but it is probably multifactorial. RA susceptibility is related to genetic, hormonal, immunologic, and environmental factors. Mannose-binding lectin (MBL) is an important protein of the human innate immune system, encoded by the MBL2 gene. Polymorphisms in MBL2 were associated with several diseases, and may be an important factor in RA susceptibility. We analyzed 3 MBL2 gene polymorphisms in 322 Brazilian patients with RA and 345 ethnically matched healthy controls.Methods.MBL2 gene variants were analyzed through polymerase chain reaction sequencing.Results.Considering MBL2 B, C, and D alleles separately, a significant difference in both genotypic and allelic frequencies, particularly concerning frequency of the C allele, was observed comparing European-derived and African-derived individuals (European-derived patients 0.022 vs African-derived patients 0.205; European-derived controls 0.029 vs African-derived controls 0.144; both p < 0.001). We also analyzed MBL2 genotype in relation to extraarticular manifestations. Considering MBL2 variants together, we found an increased frequency of the OO genotype among patients with rheumatoid nodules (p = 0.031), although this association lost significance after Bonferroni correction.Conclusion.Our findings suggest an association of MBL2 genotypes with some clinical manifestations of RA, but more studies are needed to clarify the actual role of MBL in RA.

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