Serum Human β-Defensin 2 Is Increased in Angioedema Accompanying Chronic Spontaneous Urticaria

2021 ◽  
pp. 1-6
Author(s):  
Thi Bich Tra Cao ◽  
Hyun-Young Cha ◽  
Eun-Mi Yang ◽  
Bo-Youn Choi ◽  
Hae-Sim Park ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cell Degranulation ◽  
Chronic Spontaneous Urticaria ◽  
Autologous Serum ◽  
Healthy Controls ◽  
Serum Samples ◽  
Autologous Serum Skin Test ◽  
Vitamin D Levels ◽  
Ige Mediated ◽  
Urticaria Activity Score

<b><i>Introduction:</i></b> Chronic spontaneous urticaria (CSU) is a common cutaneous disease caused by mast-cell degranulation. Human β-defensin 2 (HBD2) is a well-known antimicrobial peptide that is also a pruritogen inducing vascular permeability via non-IgE-mediated mast-cell degranulation. <b><i>Objective:</i></b> We investigated the associations between serum HBD2 levels and the clinical characteristics of CSU patients. <b><i>Methods:</i></b> Serum samples from 124 CSU patients and 56 healthy controls were screened for the levels of HBD2 and translationally controlled tumor protein (TCTP)_ by using ELISA. The urticaria activity score over 7 days (UAS7) was used to measure disease activity in CSU patients. Accompanying angioedema was self-reported. <b><i>Results:</i></b> Serum HBD2 levels were higher in the CSU group than in healthy subjects (median [interquartile range], 84.1 [43.5, 142.5] vs. 59.5 [26.7, 121.5], <i>p</i> = 0.034). In CSU patients, serum HBD2 level was negatively correlated with the peripheral basophil percentages (Spearman’s rho = −0.229, <i>p</i> = 0.01) and vitamin D levels (−0.262, <i>p</i> = 0.02), but positively correlated with TCTP levels (0.252, <i>p</i> = 0.006). In CSU patients, HBD2 level was higher in those with than without angioedema (101.7 [50.9, 184.2] vs. 66.7 [37.9, 132.0], <i>p</i> = 0.019). It did not differ by aspirin hypersensitivity or atopy status, or autologous serum skin test positivity. <b><i>Conclusion:</i></b> A known mast-cell degranulator, HBD2 was elevated in the sera from CSU patients compared to healthy controls and may be involved in the pathogenesis of accompanying angioedema.

Interleukin-23/ Interleukin -17 Axis in Chronic Spontaneous Urticaria: A Case- Control Study

2021 ◽  
Vol 30 (1) ◽  
pp. 169-174
Author(s):  
Lobna A. El-Korashi ◽  
Basma M. Elkholy ◽  
Hanaa M. El Maghraby
Keyword(s):  
Disease Severity ◽  
Serum Levels ◽  
Chronic Spontaneous Urticaria ◽  
Autologous Serum ◽  
Control Group ◽  
Interleukin 17 ◽  
Autologous Serum Skin Test ◽  
Interleukin 23 ◽  
Urticaria Activity Score ◽  
Control Study

Background: Chronic Spontaneous Urticaria (CSU) is a common health problem and its clear etiology is not established yet. Several theories have been tried to illustrate its etiology and pathogenesis. Autoantibodies and inflammatory cytokines like IL-23 and IL17 are hypothesized to take part in CSU pathogenesis and outcome. Objectives: To detect serum levels of IL-23 and IL-17A among CSU patients and to determine its correlation with disease severity and its relation to autoreactivity. Methodology: Serum levels of IL-23 and IL-17A were measured in 23 patients with CSU (CSU group) and 23 healthy controls (control group). In CSU group, Weekly Urticaria Activity Score (UAS7) was recorded to assess disease severity. Autologous Serum Skin Test (ASST) was performed to assess autoreactivity. CSU patients᾿ group was subdivided, based on ASST, into positive ASST (ASST+ ) and negative ASST (ASST- ) subgroups. Correlation of serum IL-23 & IL-17A levels, with UAS7 and ASST response were analyzed. Results: CSU group had higher serum IL-17A and IL-23 levels than control group (P=0.000). ASST+ CSU had higher serum IL-17A and IL-23 levels than ASSTones (P=0.000). Additionally, UAS7 was higher in ASST+ subgroup than ASST- subgroup (32+11.7 versus 16.27+ 9.92; P =0.005). There was significant positive correlation between disease severity and serum levels of both IL-17A and IL-23 among CSU patients (r= 0.626 & P= 0.001 and r=0.515 & P= .012, respectively). Conclusion: Increased serum IL-17A and IL-23 levels may constitute two major determinants of CSU pathogenesis and severity.

scholarly journals Interleukin 17 Receptor a Haplotype Analysis in Chronic Spontaneous Urticaria

2020 ◽  
Author(s):  
Hesham Nada ◽  
Ranya Hassan ◽  
Rasha Abd El-Hamed Ibrahim ◽  
Omnia Emad Abdelsalam ◽  
Amal Fathy ◽  
...  
Keyword(s):  
Quality Of Life ◽  
Haplotype Analysis ◽  
Complex Trait ◽  
Chronic Spontaneous Urticaria ◽  
Autologous Serum ◽  
Interleukin 17 ◽  
Allelic Discrimination ◽  
Autologous Serum Skin Test ◽  
Urticaria Activity Score

Abstract Background: Chronic spontaneous urticaria (CSU) is a distressing skin disease. Family clustering and heterogeneity in the onset and progression indicate that susceptibility to CSU is a complex trait. In this study, we performed haplotype analysis for one of the key player gene, IL17RA, for CSU to test the association with disease susceptibility and severity.MethodsThe study included 70 CSU patients and 30 healthy controls. The severity of the disease was evaluated by autologous serum skin test (ASST) and urticaria activity score (UAS). ASST test was done and quality of life was assessed using a questionnaire. Allelic discrimination analysis for rs4819554 and rs879577 was performed using Real-Time Polymerase Chain Reaction technology.Results: Carriers of rs4819554*G were more prone to develop CSU than its counterpart (p = 0.039), while rs4819554*A allele displayed more severe phenotype in the form of more prolonged disease duration (p = 0.040), concurrent angioedema (p < 0.001), higher level of treatment (p < 0.001), and higher score of quality of life (p < 0.001). Additionally, homozygote patients with rs879577*CC were associated with angioedema (p < 0.001). Haplotype analysis revealed that cohorts with both rs4819554*A and rs879577*T conferred protection against developing CSU (OR = 0.07, 95%CI = 0.01 - 0.32, p = 0.001).Conclusions: Our results showed that IL17RA gene polymorphisms might contribute to the increased susceptibility to CSU.

Inhibition of IgE-mediated mast cell degranulation by sulphasalazine

1985 ◽  
Vol 107 (2) ◽  
pp. 279-281 ◽  
Author(s):  
Kim E. Barrett ◽  
Tracy L. Tashof ◽  
Dean D. Metcalfe
Keyword(s):  
Mast Cell ◽  
Mast Cell Degranulation ◽  
Ige Mediated

IgE-mediated mast cell degranulation and recovery monitored by time-lapse photography☆

2001 ◽  
Vol 108 (1) ◽  
pp. 116-121 ◽  
Author(s):  
Zou Xiang ◽  
Mats Block ◽  
Carl Löfman ◽  
Gunnar Nilsson
Keyword(s):  
Mast Cell ◽  
Time Lapse ◽  
Mast Cell Degranulation ◽  
Ige Mediated ◽  
Time Lapse Photography

scholarly journals SLC10A4 regulates IgE-mediated mast cell degranulation in vitro and mast cell-mediated reactions in vivo

2017 ◽  
Vol 7 (1) ◽  
Author(s):  
Hanna Pettersson ◽  
Behdad Zarnegar ◽  
Annika Westin ◽  
Viktor Persson ◽  
Christiane Peuckert ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cell Degranulation ◽  
Ige Mediated

scholarly journals Denatonium Benzoate Exposure Promotes IgE-Mediated Mast Cell Degranulation and Allergy By Upregulating FcεRIα Expression

2021 ◽  
Author(s):  
Huaping Xu ◽  
Xiaoyun Shi ◽  
Mengting Xie ◽  
Shiyu Xiao ◽  
Xin Li ◽  
...  
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Cell Surface ◽  
Surface Expression ◽  
Specific Ige ◽  
Mast Cell Degranulation ◽  
Cell Surface Expression ◽  
Denatonium Benzoate ◽  
Ige Mediated

Abstract Background: Denatonium benzoate (DB), one of the bitterest compounds known to man, is currently added to a wide range of products and is also used for alcohol denaturation. Some reports demonstrated that asthmatic symptoms are associated with DB exposure but the possible links between DB and IgE-mediated allergy susceptibility have not been examined to date. We investigated the effects of DB on IgE-mediated mast cell degranulation in vitro and in the ovalbumin (OVA)-induced mouse model of allergy.Methods: DB treatments were given to RBL-2H3 IgE-sensitized rat mast cell/basophil cells and KU812 human basophilic cells together with OVA-induced allergic BALB/c mice. Allergic mediator release, Ca2+ influx and OVA-specific IgE anaphylactic shock symptoms were measured along with the cell-surface expression of the α-subunit of high-affinity IgE receptor FcεRI on mast cells.Results: DB increases β-hexosaminidase (β-hex) release and Ca2+ mobilization in IgE-mediated activated RBL-2H3 and KU812 cells, and enhanced the cell-surface expression of FcεRIα. DB also promoted the severity of OVA-induced anaphylactic and diarrheic symptoms which was accompanied by mucus thickness in jejunum and the levels of β-hex, histamine and OVA-specific IgE in allergy mice, as well as the levels of FcεRIα mRNA and the FcεRIα proteinin isolated mucosal mast cells. Conclusions: DB treatments can promote the IgE-mediated mast cell degranulation in vitro and OVA-induced allergic susceptibility in mice by upregulating mast-cell-surface FcεR1α expression, providing evidence for DB exposure in promoting allergy susceptibility.

scholarly journals Febrile urticaria in a family: uncommon manifestation of a common disease

2012 ◽  
Vol 6 (12) ◽  
pp. 895-896 ◽  
Author(s):  
Vishal Sharma ◽  
Mayank Singhal ◽  
Alka Sharma ◽  
Vivek Kumar
Keyword(s):  
Mast Cell ◽  
Vivax Malaria ◽  
Purpura Fulminans ◽  
Mast Cell Degranulation ◽  
Common Disease ◽  
Single Family ◽  
Cutaneous Manifestations ◽  
Petechial Rash ◽  
Urticarial Rash ◽  
Ige Mediated

Cutaneous manifestations are uncommon with malaria. These include urticaria, purpura fulminans, and petechial rash. We report on a series of three patients from a single family who had an urticarial rash with fever that was subsequently diagnosed to be caused by malaria. Urticarial rash has been previously reported with both falciparum and vivax malaria infections. Although the exact pathogenesis is not clear urticarial rash might be related with IgE mediated mast cell degranulation.

scholarly journals Quality of Life Based on Autologous Serum Skin Test Result in Chronic Spontaneous Urticaria Patients

2021 ◽  
Vol 4 (1) ◽  
pp. 24-29
Author(s):  
Mahvira Chow Liana Herman Adil ◽  
Nopriyati Nopriyati ◽  
Desi Oktariana ◽  
Yuli Kurniawati ◽  
Gita Dwi Prasasty
Keyword(s):  
Quality Of Life ◽  
Skin Test ◽  
Outpatient Clinic ◽  
Chronic Spontaneous Urticaria ◽  
Autologous Serum ◽  
Bivariate Analysis ◽  
Female Group ◽  
Cross Sectional ◽  
Autologous Serum Skin Test

Several studies regarding the quality of life of chronic spontaneous urticaria patients based on Autologous Serum Skin Test (ASST) results have shown a variety of results. This study aims to determine the correlation between the quality of life and ASST results in chronic spontaneous urticaria patients at Dermatology and Venereology (DV) Outpatient Clinic of Dr. Mohammad Hoesin Hospital Palembang. This analytic observational study with a cross-sectional design used secondary data in the form of medical records. 76 samples met the inclusion criteria from 110 samples of chronic spontaneous urticaria patients at DV outpatient clinic. The distribution of chronic spontaneous urticaria patients was highest in the 17-25 year age group (23.7%) and the female group (64.5%). The majority of chronic spontaneous urticaria patients had negative ASST results (52.6%). The effect of chronic spontaneous urticaria on the decline in quality of life was mostly moderate (35.5%). The bivariate analysis between DLQI score and ASST results with a value of p = 0.307 or p> 0.05 showed no significant correlation between the quality of life and ASST results.

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