Effects of washed microbiota transplantation on serum uric acid levels, symptoms and intestinal barrier function in patients with acute and recurrent gout: a pilot study

Introduction: Gut dysbiosis has been reported to be closely associated with gout. Washed microbiota transplantation (WMT) is considered as an effective way to restore a healthy gut microbiota with less adverse events than the conventional fecal microbiota transplantation. In this study, we aimed to evaluate the effects of WMT on serum uric acid levels, symptoms and the intestinal barrier function in patients with acute and recurrent gout. Methods: We performed a pilot study of WMT for acute and recurrent gout. The primary outcome was the changes in serum uric acid level and gout symptoms. The secondary outcomes included the changes in levels of diamine oxidase (DAO), D-lactic acid and endotoxin. Results: Eleven patients received WMT treatment. The averaged serum uric acid levels in patients with gout reduced after WMT (P = 0.031), accompanied with a decrease in the frequency and duration time of acute gout flares (P < 0.01). The levels of DAO, D-lactic acid and endotoxin were higher in patients than in healthy donors (P < 0.05). After WMT treatment, the levels of DAO and endotoxin decreased (P < 0.05). Conclusions: WMT is effective for reducing serum uric acid levels and improving gout symptoms in patients with gout, and contributes to improve their impaired intestinal barrier function.

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Effects of fecal microbiota transplantation on serum uric acid levels, symptoms and intestinal barrier function in patients with acute and recurrent gout: a pilot study

10.21203/rs.3.rs-41655/v1 ◽

2020 ◽

Author(s):

Wenrui Xie ◽

Xiaoya Yang ◽

Zhihe Deng ◽

Yamei Zheng ◽

Ran Zhang ◽

...

Keyword(s):

Uric Acid ◽

Lactic Acid ◽

Pilot Study ◽

Serum Uric Acid ◽

Barrier Function ◽

Fecal Microbiota Transplantation ◽

Intestinal Barrier ◽

Fecal Microbiota ◽

Intestinal Barrier Function ◽

Acute Gout

Abstract Background: Gut dysbiosis has been reported to be closely associated with gout. Fecal microbiota transplantation (FMT) has been considered as an effective way to restore the balance of gut microbiota. We aimed to evaluate the effects of FMT on serum uric acid levels, gout symptoms and the intestinal barrier function in patients with acute and recurrent gout. Methods: We performed a pilot study of FMT for acute and recurrent gout. The primary outcome was the changes in serum uric acid level on day 28 post-FMT and in gout symptoms by one year. The secondary outcomes included the changes in levels of urine uric acid, diamine oxidase (DAO), D-lactic acid and endotoxin on day 28 post-FMT. The levels of DAO, D-lactic acid and endotoxin were assessed by enzyme assay. Results: Eleven patients received FMT treatment. All the patients had a reduction in serum uric acid levels after FMT treatment ( P < 0.05), accompanied with a decrease in the frequency and duration time of acute gout flares. The levels of DAO, D-lactic acid and endotoxin, reflecting the intestinal barrier function, were higher in patients with gout than in healthy donors ( P < 0.05). After FMT treatment, the levels of DAO and endotoxin decreased ( P < 0.05). Conclusions: Our findings demonstrate that FMT is effective for reducing serum uric acid levels and improving gout symptoms in patients with gout; FMT contributes to improve the impaired intestinal barrier function of the patients.

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Effects of resistance training on serum uric acid level of hyperuricemic patients (pilot study)

GOUT AND NUCLEIC ACID METABOLISM ◽

10.6032/gnam.40.123 ◽

2016 ◽

Vol 40 (2) ◽

pp. 123-130

Author(s):

Hiroshi Ooyama ◽

Keiko Ooyama ◽

Hitoshi Moromizato ◽

Norifumi Takagi ◽

Yuki Tashiro ◽

...

Keyword(s):

Uric Acid ◽

Pilot Study ◽

Resistance Training ◽

Serum Uric Acid ◽

Uric Acid Level ◽

Acid Level ◽

Serum Uric Acid Level

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Effects of Synbiotics to Prevent Postoperative Infectious Complications in Highly Invasive Abdominal Surgery

Annals of Nutrition and Metabolism ◽

10.1159/000479920 ◽

2017 ◽

Vol 71 (Suppl. 1) ◽

pp. 23-30 ◽

Cited By ~ 2

Author(s):

Yukihiro Yokoyama ◽

Takashi Asahara ◽

Koji Nomoto ◽

Masato Nagino

Keyword(s):

Lactic Acid ◽

Abdominal Surgery ◽

Bacterial Translocation ◽

Barrier Function ◽

Intestinal Microflora ◽

Surgical Stress ◽

Controlled Trial ◽

Intestinal Barrier ◽

Short Chain Fatty Acids ◽

Intestinal Barrier Function

Postoperative infectious complication (POIC) is one of the most common complications following highly invasive abdominal surgeries, such as hepatectomy, esophagectomy, and pancreatoduodenectomy. The surgical stress temporarily deteriorates the intestinal microenvironment, and the fecal concentrations of beneficial bacteria such as Bifidobacterium and Lactobacillus decrease following highly invasive abdominal surgery. In parallel with these changes, the concentrations of fecal short-chain fatty acids (SCFAs) such as acetic acid, propionic acid, and butyric acid also decrease after surgery. In contrast, the fecal concentration of lactic acid increases under this condition because of the deterioration of the metabolism from lactic acid to SCFAs by normal intestinal microflora. Decreased fecal concentration of SCFAs may lead to an impaired intestinal barrier function under stressful condition. Translocation of bacteria from the gut to lymphatic and bloodstream leads to bacteremia and subsequent POICs. The incidence of POICs in patients with unhealthy intestinal microflora before surgery may be more because their intestine is more susceptible to bacterial translocation induced by surgical stress. Therefore, improving the intestinal microenvironment and intestinal barrier function before surgery is crucial to prevent POICs following highly invasive abdominal surgeries. In this regard, the use preoperative synbiotics therapy may be one of the effective ways because it has been shown to improve intestinal microflora, increase fecal SCFAs, prevent bacterial translocation, and reduce the incidence of POICs in several randomized controlled trial in patients undergoing highly invasive abdominal surgeries.

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Peer Review #2 of "Serum uric acid level is correlated with the clinical, pathological progression and prognosis of IgA nephropathy: an observational retrospective pilot-study (v0.1)"

10.7287/peerj.10130v0.1/reviews/2 ◽

2020 ◽

Keyword(s):

Uric Acid ◽

Pilot Study ◽

Peer Review ◽

Iga Nephropathy ◽

Serum Uric Acid ◽

Uric Acid Level ◽

Acid Level ◽

Serum Uric Acid Level

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Gut microbiota regulates AML via alternation of intestinal barrier function mediated by butyrate

10.21203/rs.3.rs-157712/v1 ◽

2021 ◽

Author(s):

Ruiqing Wang ◽

Xinyu Yang ◽

Jinting Liu ◽

Fang Zhong ◽

Chen Zhang ◽

...

Keyword(s):

Gut Microbiota ◽

Barrier Function ◽

Fecal Microbiota Transplantation ◽

Therapeutic Option ◽

Leukemia Cell ◽

Intestinal Barrier ◽

Intestinal Barrier Function ◽

Significant Shift ◽

Dependent Manner ◽

Intestinal Microbes

Abstract The gut microbiota has been linked to many cancers, yet the role of intestinal microbes in acute myeloid leukemia (AML) progression remains unclear. Here, we observed a significant shift in the gut microbiota in AML patients, characterized by reduced Faecalibacterium abundance. According to a murine AML model, we found that intestinal microbial diversity decreased as the disease progressed. On the other side, gut microbiota dysbiosis induced by antibiotic treatment accelerated AML progression with a higher leukemia cell burden and shorter overall survival (OS), while fecal microbiota transplantation altered this process. Metabolome analyses showed that microbiota-derived butyrate concentration obviously decreased in AML patient feces, and butyrate gavage postponed AML progression in a mouse model. Moreover, our study revealed that intestinal barrier function is decreased in AML mice which may be related to the microbiota disorder caused by AML. Lower intestinal barrier function increased the bacterial-associated lipopolysaccharide (LPS) concentration in the peripheral blood of AML patients or mice through enhancing intestinal permeability. Butyrate repaired the intestinal barrier damage and inhibited LPS absorption in AML mice. Collectively, these findings demonstrate that the gut microbiota promotes AML progression in a metabolite dependent manner, and targeting the gut microbiota might provide a novel therapeutic option for AML.

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Estimation of Serum Uric Acid in Cases of Hyperuriceamia and Gout

Journal of Nepal Medical Association ◽

10.31729/jnma.30 ◽

2011 ◽

Vol 51 (181) ◽

Author(s):

R K Pokharel ◽

BK Yadav ◽

B Jha ◽

K Parajuli

Keyword(s):

Uric Acid ◽

Serum Uric Acid ◽

Uric Acid Level ◽

Acid Level ◽

Serum Uric Acid Level ◽

Relevant Information ◽

University Teaching ◽

Acute Gout ◽

College Hospital ◽

The Mean

Introduction: Gout is crystal deposit arthritis and is an ancient disease. The biologic precursor to gout is hyperuricaemia. The prevalence of hyperuricaemia and gout has an increasing trend all over the world including the developing countries. The purpose of this study is to estimate serum uric acid level in hyperuricaemic and gout patients attending a medical college hospital. Methods: A consecutive 150 hyperuricaemics and 150 gout patients attending Tribhuwan University Teaching Hospital from June to September 2005 were included in this study. The serum uric acid level was measured by the enzymatic (PAP- Uricase) method. The patients with acute gout were interviewed and relevant information was obtained. Results: Males comprised 84 % of gout cases. Hyperuricaemia was common in both sexes. The mean age for gout was 47.49 and 56.65 years in males and females respectively. The mean age for the first gout attack was 42.1 ± 14.0 years. Family history was positive in 22 % of cases. The overall mean serum uric acid level in hyperuricaemics was 7.2 ± 0.7 mg/dL and 8.4 ± 1.1 mg/dL in acute gout (p 0.0001). The mean serum uric acid level was signifi cantly (p 0.0001) high among males both at the asymptomatic phase and at acute gout. Gout was more common in non-vegetarians (95 %) and alcoholics (65.3 %). Serum uric acid level was inversely related with the amount of daily water intake (p 0.0001). Conclusions: Serum uric acid level is signifi cantly high among the male gouty arthritic patients. However, it is also high among asymptomatic hyperuricaemic cases of both sexes. Keywords: asymptomatic hyperuricaemia, gout, serum uric acid level.

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Early Intervention Using Fecal Microbiota Transplantation Combined with Probiotics Influence the Growth Performance, Diarrhea, and Intestinal Barrier Function of Piglets

Applied Sciences ◽

10.3390/app10020568 ◽

2020 ◽

Vol 10 (2) ◽

pp. 568 ◽

Cited By ~ 1

Author(s):

Quanhang Xiang ◽

Xiaoyu Wu ◽

Ye Pan ◽

Liu Wang ◽

Yuwei Guo ◽

...

Keyword(s):

Early Intervention ◽

Growth Performance ◽

Relative Abundance ◽

Barrier Function ◽

Fecal Microbiota Transplantation ◽

Bacterial Colonization ◽

Average Daily Gain ◽

Intestinal Barrier ◽

Fecal Microbiota ◽

Intestinal Barrier Function

Early intervention with fecal microbiota transplantation (FMT) improves the growth performance and intestinal barrier function of piglets. Accelerating intestinal oxygen concentration is beneficial for symbiotic bacterial colonization. Saccharomyces boulardii (SB) is an aerobic fungus, which may contribute to the colonization of anaerobic symbiotic bacteria by competing for oxygen. Clostridium butyricum (CB) improves intestinal barrier function and performance, via regulating the gut microbiota composition of piglets. The objective of this study was to investigate the effect of early intervention with FMT combining CB and SB on growth performance, diarrhea, and intestinal barrier function in piglets. A total of 77 litters of neonatal piglets assigned to one of six treatments, which treated with antibiotics (AB), placebo (CON), and FMT (FMT), FMT-added CB (FMT+C), FMT-added SB (FMT+S), and FMT-added CB and SB (FMT+C+S), respectively. FMT+C+S treated piglets had higher body weight (BW) and average daily gain (ADG) both in weaning and finial period, and it significantly increased the levels of fecal mucin-2 (MUC2), fecal short-chain fatty acids (SCFAs), and relative abundance of fecal Lactobacillus spp., and Bifidobacterium genus. Moreover, early intervention with FMT+C+S reduced the diarrhea rate during the experiment. FMT+C+S also decreased the level of plasma diamine oxidase (DAO) and D-lactate (D-LA), and relative abundance of fecal E. coli during the suckling period. In summary, early intervention with FMT combining CB and SB improved the growth performance, intestinal barrier function, fecal SCFAs concentration, and fecal Lactobacillus and Bifidobacterium of piglets.

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