scholarly journals Potential and Performance of Accelerated Solvent Extraction (ASE) in Obtaining Bioactive Compounds from Bee Propolis: Comparison with Soaking, Ultrasonication, and Microwave-Assisted Methods

2021 ◽  
Vol 11 (2) ◽  
pp. 187-192
Author(s):  
Elmer-Riko Mojica ◽  
◽  
Jozlyn Charland ◽  
Custer Deocaris ◽  
Jose Micor ◽  
...  
Keyword(s):  
Natural Products ◽  
Solvent Extraction ◽  
Bioactive Compounds ◽  
Biological Properties ◽  
Accelerated Solvent Extraction ◽  
Gas Chromatography Mass Spectrometry ◽  
Spectroscopic Techniques ◽  
Extraction Temperature ◽  
Food Component ◽  
Microwave Assisted

Propolis is a natural resinous substance collected by honeybees from buds and exudates of trees. The material has attracted much attention in recent years as a functional food component since it possesses various biological properties, including antimicrobial, antioxidative, and anti-ulcer properties. In this study, the performance of accelerated solvent extraction (ASE) was assessed and compared with varying methods of extraction: soaking (maceration), ultrasonication, and microwave-assisted methods. Gas chromatography-mass spectrometry (GC-MS) and other spectroscopic techniques, such as absorbance and fluorescence, were employed to assess the efficiency in the extraction of natural products. The antioxidant activity and phenolic content of the different extracts were also determined. Results showed samples obtained from the microwave method showed the highest yield in the extraction of bioactive compounds. Although microwave showed the best method in this study, some issues and recommendations on ASE application for extracting natural products from bee propolis were discussed. Given the ease in controlling extraction temperature with ASE, this technique has a great potential to be a better method for extraction of heat-labile natural products from propolis should optimization of conditions for extraction were further performed.

scholarly journals Accelerated solvent extraction of bioactive compounds from carrot: Optimization of response surface methodology

2018 ◽  
Vol 83 (11) ◽  
pp. 1223-1228 ◽  
Author(s):  
Vanja Seregelj ◽  
Vesna Tumbas-Saponjac ◽  
Anamarija Mandic ◽  
Gordana Cetkovic ◽  
Jasna Canadanovic-Brunet ◽  
...  
Keyword(s):  
Response Surface Methodology ◽  
Solvent Extraction ◽  
Response Surface ◽  
Bioactive Compounds ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Accelerated Solvent Extraction ◽  
Carotenoid Content ◽  
Extraction Temperature ◽  
Significant Difference

Carrot is considered to be rich in bioactive antioxidants, both lipophilic (carotenoids) and hydrophilic (phenolic compounds). In the present study, the conditions for accelerated solvent extraction (ASE) of bioactive compounds from carrots (Daucus carota L.) were optimized using response surface methodology (RSM). Box?Behnken design was employed for the experimental design to obtain the optimized combination of extraction temperature, time, and number of extraction cycles. Total carotenoid content (TCar), total polyphenol content (TPh), free radical scavenging activity (SA) and reducing power (RP) of the obtained extracts were used as responses for the optimization. Considering the four quality indicators, the ideal extraction conditions were found to be: 120 ?C, 60 min and three extraction cycles. Under these conditions, predicted values of 28.84 mg ?-carotene/100 g for TCar; 530.81 mg GAE/100 g for TPh; 2572.29 ?mol TE/100 g for SA and 1336.26 ?mol TE/100 g for RP were obtained with high desirability (0.975) and no significant difference (p < 0.05) with the experimental values.

Microwave-Assisted Solvent Extraction of Melamine from Seafood and Determination by Gas Chromatography–Mass Spectrometry: Optimization by Factorial Design

2014 ◽  
Vol 47 (7) ◽  
pp. 1118-1133 ◽  
Author(s):  
Cristina Truzzi ◽  
Silvia Illuminati ◽  
Carolina Finale ◽  
Anna Annibaldi ◽  
Carmela Lestingi ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Solvent Extraction ◽  
Factorial Design ◽  
Gas Chromatography Mass Spectrometry ◽  
Microwave Assisted ◽  
Chromatography Mass Spectrometry

scholarly journals Determination of Organochlorine Pesticides and Chlorobenzenes in Strawberries by Using Accelerated Solvent Extraction Combined with Sorptive Enrichment and Gas Chromatography/Mass Spectrometry

2001 ◽  
Vol 84 (4) ◽  
pp. 1194-1201 ◽  
Author(s):  
Luise Wennrich ◽  
Peter Popp ◽  
Gábor Köller ◽  
Jürgen Breuste
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Solvent Extraction ◽  
Organochlorine Pesticides ◽  
Accelerated Solvent Extraction ◽  
Stir Bar Sorptive Extraction ◽  
Mass Spectrometry Analysis ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

Abstract An analytical scheme for the determination of several organochlorine pesticides like hexachlorocyclohexanes (HCHs) and DDX compounds (p,p′-DDE, p,p′-DDD, and p,p′-DDT) as well as chlorobenzenes in strawberries has been developed. The procedure is based on aqueous accelerated solvent extraction (ASE) followed by solidphase microextraction (SPME) or stir bar sorptive extraction (SBSE) and subsequent thermodesorption–gas chromatography/mass spectrometry analysis. A 65 μm polydimethylsiloxane/divinylbenzene fiber was chosen for the SPME experiments. Significant SPME and ASE parameters were optimized using spiked water and strawberry samples. For the ASE of the organochlorine compounds, a water–acetone mixture (90 + 10, v/v) as the extraction solvent, an extraction temperature of 120°C, and 2 cycles of 10 min extraction proved optimal. The developed method was evaluated with respect to precision and limits of detection (LOD). The relative standard deviations of replicate ASE–SPME determinations (n = 5) were in the range of 4–24%. LOD values between 1 and 10 μ g/kg were achieved with the exception of DDT and DDE (40 μg/kg). Using SBSE, the LOD of these compounds could be improved (2 and 5 μg/kg). The main advantages of this method are the avoidance of cleanup and concentration procedures as well as the significant reduction of the required volume of organic solvents. The described method was applied to the determination of the pollutants in strawberry samples collected from different allotment gardens in a potentially polluted area, the Bitterfeld-Wolfen region (Germany).

Analysis of Cembranoids in Flue-cured Tobacco by Accelerated Solvent Extraction and Gas Chromatography-Mass Spectrometry-Selected Ion Monitoring

2014 ◽  
Vol 61 (10) ◽  
pp. 1133-1140 ◽  
Author(s):  
Xiao-Bo Qian ◽  
Jiang-Ping Ye ◽  
Xiao-Ming Chen ◽  
Chang-Hua Zhang ◽  
Yong-Jiang Liang ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Solvent Extraction ◽  
Accelerated Solvent Extraction ◽  
Gas Chromatography Mass Spectrometry ◽  
Selected Ion Monitoring ◽  
Chromatography Mass Spectrometry

Volatile and semi-volatile components of oak wood chips analysed by Accelerated Solvent Extraction (ASE) coupled to gas chromatography–mass spectrometry (GC–MS)

2007 ◽  
Vol 102 (4) ◽  
pp. 1260-1269 ◽  
Author(s):  
Stefania Vichi ◽  
Cecilia Santini ◽  
Nadia Natali ◽  
Claudio Riponi ◽  
Elvira López-Tamames ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Solvent Extraction ◽  
Accelerated Solvent Extraction ◽  
Wood Chips ◽  
Gas Chromatography Mass Spectrometry ◽  
Volatile Components ◽  
Oak Wood ◽  
Chromatography Mass Spectrometry

scholarly journals Original Article BIOACTIVE COMPOUNDS FROM SPONGE SUBERITES CARNOSUS (JOHNSTON) COLLECTED FROM WEST COAST OF MUMBAI, INDIA

2021 ◽  
pp. 41-51
Author(s):  
BHADEKAR N. S ◽  
ZODAPE G. V.
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Bioactive Compounds ◽  
West Coast ◽  
Acid Methyl Ester ◽  
Biological Properties ◽  
Gas Chromatography Mass Spectrometry ◽  
Ftir Analysis ◽  
Infrared Spectrometer ◽  
Layer Chromatography

Objective: Structural elucidation of bioactive compounds from marine Sponge Suberites Carnosus (Johnston) by using Thin Layer Chromatography (TLC), Gas Chromatography-Mass Spectrometry (GC-MS), Fourier Transform Infrared Spectrometer (FTIR) techniques with respect to its pharmacological and biomedical properties. Methods: The sponge Suberites carnosus (Johnston) was collected during low tides from West Coast of Mumbai. Crude extract was obtained by taking 10 gram of sponge sample in10 ml of methanol. The preparative TLC (Thin Layer Chromatography) was performed by using Toluene: Ethyl acetate: Diethylamine (7:2:1) (v/v). The isolated compounds were subjected to GC-MS and FTIR analysis. Results: From the GC-MS and FTIR analysis, total ten compounds were identified. The GC-MS spectra correlate to the mass spectra. The structures of those compounds are 6-Fluoro 2-trifluromethylbenzoic acid,2,3-dichlorophenyl ester, Eicosane 3-cyclohexyl, Phosphine imide, P,P,P,-tris (p-chlorophenyl)-N-phenyl-, Dimethylhyl hexavinyl octasilsesquioxane, Hexanoicacid, hexadecyl ester, Hexadecanoic acid,2-hydroxy1-(hydroxymethyl)ethyl ester, 9, 19-Cyclolanostan-3-ol, acetate, (3β), Tetracosane, 3-ethyl-, 11, 14-Eicosadienoic acid, methyl ester, Triacontane, 11,20-didecyl-respectively. Conclusion: The nature and biological properties of the said compounds were determined and it was found that some of them act as a skin irritant. Some of them have fatty, metabolite, and irritant property, whereas some are act as masking and perfuming agents. Some compounds are highly toxic property, whereas others have an effect on health and environmental hazard. Some are highly corrosive in nature, whereas others are Cholesterol and chemotaxonomic significance.

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