Comparative evaluation of antimicrobial activity of miswak, propolis, sodium hypochlorite and saline as root canal irrigants by microbial culturing and quantification in chronically exposed primary teeth

Introduction:As the root canal system considered to be complex and unpredictable, using root canal irrigants and medicaments are essential in order to enhance the disinfection of the canal. Sodium hypochlorite is the most common irrigant in endodontics. Despite its excellent antimicrobial activity and tissue solubility, sodium hypochlorite lacks some important properties such as substantivity and smear layer removing ability.Objective:The aim of this review was to address benefits and drawbacks of combining sodium hypochlorite with other root canal irrigants and medicaments.Discussion:According to the reviewed articles, NaOCl is the most common irrigation solution in endodontics. However, it has some drawbacks such as inability to remove smear layer. One of the drawbacks of NaOCl is its inability to remove the smear layer and lack of substantivity.Conclusion:The adjunctive use of other materials has been suggested to improve NaOCl efficacy. Nevertheless, further studies are required in this field.

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Antimicrobial activity of root canal irrigants in primary teeth – Comment

GERMS ◽

10.11599/germs.2012.1016 ◽

2012 ◽

Vol 2 (2) ◽

pp. 79-79

Author(s):

Theodor-Cristian Vizitiu

Keyword(s):

Antimicrobial Activity ◽

Root Canal ◽

Primary Teeth ◽

Root Canal Irrigants

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Antimicrobial Activity of Root Canal Irrigants associated with Cetrimide against Biofilm and Planktonic Enterococcus faecalis

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1586 ◽

2014 ◽

Vol 15 (5) ◽

pp. 603-607 ◽

Cited By ~ 3

Author(s):

Mario Tanomaru-Filho ◽

Camila Almeida Nascimento ◽

Norberto Batista Faria-Junior ◽

Gisele Faria ◽

Juliane Maria Guerreiro-Tanomaru

Keyword(s):

Antimicrobial Activity ◽

Antibacterial Activity ◽

Enterococcus Faecalis ◽

Sodium Hypochlorite ◽

Root Canal ◽

Direct Contact ◽

Culture Collection ◽

Planktonic Cells ◽

Colony Forming Unit ◽

Root Canal Irrigants

ABSTRACT Aim To evaluate the antibacterial activity of sodium hypochlorite (NaOCl) and chlorhexidine (CHX) alone or associated with cetrimide (CTR), and QMiX against biofilm and planktonic Enterococcus faecalis (E. faecalis) [American type culture collection (ATCC) 29212]. Materials and methods The solutions 2.5% NaOCl, 2.5% NaOCl + 0.2% CTR, 2% CHX, 2% CHX + 0.2% CTR, 0.2% CTR, and QMiX were evaluated. E. faecalis biofilms were induced for 14 days on bovine dentin blocks. The irrigants were evaluated after contact with E. faecalis suspension and biofilm for 1 and 3 minutes. After that, serial decimal dilutions were made and plated on tryptic soy agar (TSA) medium. Plates were incubated for 24 hours at 37°C and the colony-forming unit (CFU) 1 ml was determined. Data were subjected to ANOVA and Tukey's tests at 5% significance. Results All microorganisms were eliminated by direct contact of the irrigants with planktonic cells. Only NaOCl and NaOCl + CTR were able to completely eliminate the microorganisms by direct contact with E. faecalis biofilm. CHX presented effectiveness similar to CHX + CTR CTR, and QMiX after 1 minute of contact and similar to NaOCl and NaOCl + CTR after 3 minutes (p > 0.05), but was unable to completely eliminate the microorganisms. CTR and QMiX did not differ from each other. Conclusion CTR addition to CHX and NaOCl solutions did not improve the antimicrobial activity against biofilm. All evaluated irrigants and associations presented activity against planktonic E. faecalis. Only NaOCl and NaOCl + CTR eliminated biofilm after 1 and 3 minutes of direct contact. Clinical relevance Addition of CTR does not modify the antibiofilm effectiveness of CHX and NaOCl. How to cite this article Nascimento CA, Tanomaru-Filho M, Faria-Junior NB, Faria G, Guerreiro-Tanomaru JM. Antimicrobial Activity of Root Canal Irrigants associated with Cetrimide against Biofilm and Planktonic Enterococcus Faecalis. J Contemp Dent Pract 2014;15(5):603-607.

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Comparative evaluation of antimicrobial effect of herbal root canal irrigants (Morinda citrifolia, Azadirachta indica, Aloe vera) with sodium hypochlorite: An in vitro study

Journal of International Society of Preventive and Community Dentistry ◽

10.4103/2231-0762.183104 ◽

2016 ◽

Vol 6 (3) ◽

pp. 196 ◽

Cited By ~ 7

Author(s):

Prashant Babaji ◽

Kiran Jagtap ◽

Himani Lau ◽

Nandita Bansal ◽

S Thajuraj ◽

...

Keyword(s):

Sodium Hypochlorite ◽

Azadirachta Indica ◽

Root Canal ◽

Comparative Evaluation ◽

Aloe Vera ◽

In Vitro Study ◽

Antimicrobial Effect ◽

Morinda Citrifolia ◽

Root Canal Irrigants

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The role of intracanal medicaments in inhibition of bacteria isolated from root canals of infected primary molars

Mustansiria Dental Journal ◽

10.32828/mdj.v14i1.766 ◽

2019 ◽

Vol 14 (1) ◽

pp. 92

Author(s):

Dr. Maha Abdul- Kareem Mahmood ◽

Dr. Huda Elias Ali ◽

Dr. Haraa Khairi Abdul-Kadher

Keyword(s):

Antimicrobial Activity ◽

Streptococcus Mutans ◽

Normal Saline ◽

Root Canal ◽

Primary Teeth ◽

Primary Molars ◽

Root Canals ◽

Etiologic Agents ◽

Micro Organisms

Microbes are considered as the primary etiologic agents in endodontic diseases.Disinfection of the root canal is obtained by the combined effect of biomechanicalpreparation, irrigation and intra canal medicament. The aim of the present study wasto assess the antimicrobial activity of intracanal medicaments (formocresol andEndosepton) against two micro organisms (Streptococcus mutans and staphylococcusaureus) isolated from 15 necrotic pulps of primary molars indicated for pulpectomyprocedure. The samples were cultured, and purified using microbiological evaluation.Broth dilution test was performed in our study by preparing test tubes containing10 ml of BHI broth (pH. 7) which then inoculated with strains of the tested bacteriaand incubated at 37 C° for 24 h. After over night incubaction, ten fold dilution weremade in test tubes containing 9 ml of normal saline by adding 1 ml of the inoculum tothe first tube . Then from dilution 10-1 , 0.1 ml of cell suspension was added to 9.9 mlof formocresol and endosepton, then 0.1 ml was taken and spread on duplicates ofBHI agar plates at different intervals and incubated aerobically for 24 h. at 37 C°.Colonies on the plates were counted after incubation and CFU/mL (colony formingunit) was calculated. Our results indicating that there were no significant differencesbetween the intracanal medicaments, but there were high significant differencesbetween the intervals time of the study. We concluded that both materials had greatantibacterial effect against the pathogens commonly isolated from necrotic pulpaltissue of primary teeth.

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