Desenvolvimento e caracterização de marcadores microssatélites para Puccinia melanocephala, agente causador da ferrugem marrom em cana-de-açúcar

Abstract A new distribution map is provided for Puccinia melanocephala H. & P. Sydow. Hosts: Sugar cane (Saccharum), Erianthus spp. Information is given on the geographical distribution in AFRICA, Cameroon, Kenya, Madagascar, Malawi, Mauritius, Reunion, South Africa, Tanzania, Uganda, Zambia, Zimbabwe, ASIA, China (Hupeh, Yunnan), India (Andhra Pradesh, Himachal Pradesh, Madras, Maharashta, Mysore, Punjab, Uttar Pradesh), Nepal, Taiwan, AUSTRALASIA & OCEANIA, Australia (Queensland), (New South Wales), NORTH AMERICA, Mexico, USA (Florida, La), (Mississippi), CENTRAL AMERICA & WEST INDIES, Barbados, Belize, Cuba, Dominican Republic, Guadeloupe, Guatemala, Haiti, Honduras, Jamaica, Panama, Puerto Rico, Salvador, Trinidad, SOUTH AMERICA, Guyana, Venezuela.

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Variability in Puccinia melanocephala Pathogenicity and Resistance in Sugarcane Cultivars

Plant Disease ◽

10.1094/pdis-01-14-0074-re ◽

2014 ◽

Vol 98 (12) ◽

pp. 1728-1732 ◽

Cited By ~ 9

Author(s):

J. W. Hoy ◽

M. C. Avellaneda ◽

J. Bombecini

Keyword(s):

Leaf Area ◽

Quantitative Resistance ◽

Durable Resistance ◽

Lesion Size ◽

Brown Rust ◽

Host Genotype ◽

Disease Response ◽

Puccinia Melanocephala ◽

Pathogen Variability ◽

Pathogenic Variability

Brown rust, caused by Puccinia melanocephala, is an important disease of sugarcane worldwide, controlled primarily with host plant resistance. Disease response shifts from resistant to susceptible have been repeatedly observed for cultivars. However, information is limited concerning pathogen variability related to host reactions. To evaluate variability in the pathogen population and characterize resistance responses in different host genotypes, seven cultivars were inoculated with four urediniospore collections from three cultivars. Greenhouse-grown plants were inoculated under controlled conditions favorable for infection and disease development. Severity assessed as leaf area occupied by lesions, lesion density, and lesion size was determined and compared. Three cultivars that shifted from resistance to high susceptibility while under cultivation exhibited differential disease severity when inoculated with spore collections from two of the respective cultivars. Two cultivars exhibited consistent moderate to high levels of quantitative resistance against all spore collections and two cultivars, including one with the Bru1 resistance gene, were highly resistant to all collections. Differential reactions were best revealed by assessing percent leaf area. Pathogenic variability related to host genotype was confirmed, and quantitative resistance was detected that could be useful to improve breeding and selection for effective, durable resistance to brown rust.

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Validação de marcadores moleculares associados à resistência à ferrugem marrom em cana-de-açúcar

Summa Phytopathologica ◽

10.1590/0100-5405/168917 ◽

2017 ◽

Vol 43 (1) ◽

pp. 36-40

Author(s):

Fernanda Zatti Barreto ◽

Thiago Willian Almeida Balsalobre ◽

Roberto Giacomini Chapola ◽

Hermann Paulo Hoffmann ◽

Monalisa Sampaio Carneiro

Keyword(s):

Saccharum Spp ◽

Puccinia Melanocephala

RESUMO A ferrugem marrom causada pelo fungo Puccinia melanocephala é uma importante doença em cana-de-açúcar (Saccharum spp.) e está presente em praticamente todas as áreas de cultivo. Um gene de efeito maior, Bru1, foi descrito como fonte de resistência durável da cana-de-açúcar contra isolados de P. melanocephala. O objetivo deste estudo foi avaliar a eficiência de dois marcadores moleculares fortemente associados ao gene Bru1, R12H16 e 9O20-F4-RsaI, para predição da resistência genética à ferrugem marrom em variedades de cana-de-açúcar. Para tanto, foram utilizadas neste estudo 14 variedades RB, desenvolvidas pela RIDESA (Rede Interuniversitária para o Desenvolvimento do Setor Sucroenergético), as quais estão entre as 10 mais cultivadas na região Centro-Sul do Brasil no período de 1974 a 2015. Para avaliação da doença a campo foi conduzido um experimento em blocos casualizados com quatro repetições. A severidade da ferrugem marrom foi quantificada de acordo com escala diagramática que varia de 1 (mais resistente) a 9 (mais suscetível) e os dados obtidos foram analisados através de modelo misto linear generalizado (GLMM). Concomitantemente, foi extraído o DNA total das 14 variedades e, em seguida, foi realizada amplificação dos marcadores moleculares, que foram classificados em haplótipos. Do total de variedades, 12 não mostraram sintomas da doença e, através da análise via GLMM, apresentaram probabilidade de 99,35% de serem resistentes; destas, 91,66% foram haplótipo 1, ou seja, tiveram a presença de ambos os marcadores associados ao gene Bru1. Portanto, pode-se concluir que os marcadores moleculares R12H16 e 9O20-F4-RsaI foram altamente eficientes em predizer fenótipos resistentes e que provavelmente o gene Bru1 seja a principal fonte de resistência de cana-de-açúcar à ferrugem marrom nos campos comerciais do Brasil.

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Putative resistance-associated genes induced in sugarcane in response to the brown rust fungus, Puccinia melanocephala and their use in genetic diversity analysis of Louisiana sugarcane clones

Plant Gene ◽

10.1016/j.plgene.2018.04.002 ◽

2018 ◽

Vol 14 ◽

pp. 20-28 ◽

Cited By ~ 3

Author(s):

Mavir Carolina Avellaneda ◽

Arnold P. Parco ◽

Jeffrey W. Hoy ◽

Niranjan Baisakh

Keyword(s):

Genetic Diversity ◽

Rust Fungus ◽

Brown Rust ◽

Diversity Analysis ◽

Genetic Diversity Analysis ◽

Puccinia Melanocephala

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Resistance to rust and smut among Puerto Rico sugarcane clones

The Journal of Agriculture of the University of Puerto Rico ◽

10.46429/jaupr.v73i1.6372 ◽

1969 ◽

Vol 73 (1) ◽

pp. 45-49

Author(s):

Rodrigo Echávez-Badel ◽

Jorge L. Rodríguez ◽

Carlos E. Ortiz

Keyword(s):

Puerto Rico ◽

Rust Resistance ◽

Puccinia Melanocephala ◽

Ustilago Scitaminea

One hundred and ninety-two sugarcane clones were evaluated for resistance to rust (Puccinia melanocephala) H. Syd. and P. Syd.) and to smut (Ustilago scitaminea Syd.), between March 1985 and March 1988. Fiftynine clones (31%) were resistant to both rust and smut in plant cane, and in the first and second ratoon crops. Incidence of smut increased after ratooning. Some 81 to 96% were resistant to rust. Rust resistance remained unchanged in plant cane and ratoon crops.

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