Low Carbohydrate/High-Fat Diet Attenuates Cardiac Hypertrophy, Remodeling, and Altered Gene Expression in Hypertension

In the obese state, enlarged adipose cells display an altered gene-expression profile and metabolic capacity. The aim of this study was to gain insight into their secretory function, by assessing two secreted proteins, leptin and angiotensinogen, in adipose cells of obese (fa/fa) Zucker rats. A marked and co-ordinate increase in leptin mRNA, gene transcription and promoter activity was observed in obese compared with lean (Fa/fa) rat adipose cells, and this resulted in increased leptin release in culture. Two sets of observations suggest that this effect is due to the fa mutation. First, adipose-cell leptin release was higher in heterozygous (Fa/fa) than in homozygous (Fa/Fa) lean rats. Second, leptin release was not enhanced in enlarged adipose cells of FalFa rats fed a high-fat diet for 15 days. At variance with leptin, angiotensinogen production was not significantly increased in the obese cells. Dexamethasone stimulated both leptin and angiotensinogen release in lean and obese rat adipose cells. The magnitude of leptin stimulation was higher in fa/fa than in Fa/fa rats, whereas angiotensinogen release was increased to the same extent in both genotypes. These observations suggest that leptin production is specifically enhanced in enlarged adipose cells of obese Zucker rats and that cell hypertrophy is not the sole determinant of this feature. Increased leptin production might be related to disruption of leptin signalling by the fa mutation.

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IMPACT OF HIGH FAT DIET ON MYOCARDIAL GENE EXPRESSION IN CARDIAC HYPERTROPHY

The FASEB Journal ◽

10.1096/fasebj.21.6.a1409-a ◽

2007 ◽

Vol 21 (6) ◽

Author(s):

Anne‐Marie L Seymour ◽

Ashwin Akki

Keyword(s):

Gene Expression ◽

Cardiac Hypertrophy ◽

High Fat Diet ◽

High Fat ◽

Myocardial Gene Expression

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Effect of low carbohydrate high fat diet on LDL cholesterol and gene expression in normal-weight, young adults: A randomized controlled study

Atherosclerosis ◽

10.1016/j.atherosclerosis.2018.10.013 ◽

2018 ◽

Vol 279 ◽

pp. 52-61 ◽

Cited By ~ 17

Author(s):

Kjetil Retterstøl ◽

Mette Svendsen ◽

Ingunn Narverud ◽

Kirsten B. Holven

Keyword(s):

Gene Expression ◽

Young Adults ◽

High Fat Diet ◽

Ldl Cholesterol ◽

Normal Weight ◽

Randomized Controlled Study ◽

Controlled Study ◽

High Fat ◽

Randomized Controlled ◽

Low Carbohydrate

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Altered gene expression in cardiac hypertrophy

Journal of Molecular and Cellular Cardiology ◽

10.1016/0022-2828(92)90154-r ◽

1992 ◽

Vol 24 ◽

pp. 41 ◽

Cited By ~ 1

Author(s):

Ketty Schwartz

Keyword(s):

Gene Expression ◽

Cardiac Hypertrophy ◽

Altered Gene Expression ◽

Altered Gene

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Molecular and Microscopic Analysis of Altered Gene Expression in Transgenic and Gene Targeted Mice

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100162521 ◽

1996 ◽

Vol 54 ◽

pp. 12-13

Author(s):

W. K. Jones ◽

J. Robbins

Keyword(s):

Gene Expression ◽

Pulmonary Veins ◽

Microscopic Analysis ◽

Mouse Tissue ◽

Adult Heart ◽

Heavy Chains ◽

Altered Gene Expression ◽

Altered Gene ◽

Pulmonary Myocardium

Two myosin heavy chains (MyHC) are expressed in the mammalian heart and are differentially regulated during development. In the mouse, the α-MyHC is expressed constitutively in the atrium. At birth, the β-MyHC is downregulated and replaced by the α-MyHC, which is the sole cardiac MyHC isoform in the adult heart. We have employed transgenic and gene-targeting methodologies to study the regulation of cardiac MyHC gene expression and the functional and developmental consequences of altered α-MyHC expression in the mouse.We previously characterized an α-MyHC promoter capable of driving tissue-specific and developmentally correct expression of a CAT (chloramphenicol acetyltransferase) marker in the mouse. Tissue surveys detected a small amount of CAT activity in the lung (Fig. 1a). The results of in situ hybridization analyses indicated that the pattern of CAT transcript in the adult heart (Fig. 1b, top panel) is the same as that of α-MyHC (Fig. 1b, lower panel). The α-MyHC gene is expressed in a layer of cardiac muscle (pulmonary myocardium) associated with the pulmonary veins (Fig. 1c). These studies extend our understanding of α-MyHC expression and delimit a third cardiac compartment.

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