Tyrosine Kinase Signaling Pathways Modulate Angiotensin II–Induced Calcium ([Ca2+]i) Transients in Vascular Smooth Muscle Cells

Hypertension ◽  
1996 ◽  
Vol 27 (5) ◽  
pp. 1097-1103 ◽  
Author(s):  
R.M. Touyz ◽  
E.L. Schiffrin
Keyword(s):  
Smooth Muscle ◽  
Angiotensin Ii ◽  
Vascular Smooth Muscle ◽  
Tyrosine Kinase ◽  
Smooth Muscle Cells ◽  
Signaling Pathways ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Kinase Signaling ◽  
Tyrosine Kinase Signaling

Activation of Akt/protein kinase B after stimulation with angiotensin II in vascular smooth muscle cells

1999 ◽  
Vol 276 (6) ◽  
pp. H1927-H1934 ◽  
Author(s):  
Tomosaburo Takahashi ◽  
Takahiro Taniguchi ◽  
Hiroaki Konishi ◽  
Ushio Kikkawa ◽  
Yuichi Ishikawa ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Angiotensin Ii ◽  
Protein Kinase ◽  
Vascular Smooth Muscle ◽  
Tyrosine Kinase ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Protein Kinase B ◽  
Muscle Cells ◽  
Ang Ii

Involvement of Akt/Protein kinase B (PKB), a serine/threonine kinase with a pleckstrin-homology domain, in angiotensin II (ANG II)-induced signal transduction was investigated in cultured vascular smooth muscle cells (VSMC). Stimulation of the cells with ANG II led to a marked increase in the kinase activity of Akt/PKB, which coincided with Ser-473 phosphorylation. ANG II-stimulated Akt/PKB activation was rapid, concentration dependent, and inhibited by the AT1-receptor antagonist CV-11974, but not by pertussis toxin. Akt/PKB activity was stimulated by the Ca2+ ionophore ionomycin, suggesting the possible involvement of Ca2+ in ANG II-stimulated Akt/PKB activation. However, blockade of Ca2+ mobilization by BAPTA-AM only partially inhibited ANG II-stimulated Akt/PKB activation. ANG II-stimulated Akt/PKB activation was inhibited by the tyrosine kinase inhibitors genistein and herbimycin A and by the phosphatidylinositol 3-kinase (PI3K) inhibitors wortmannin and LY-294002. These results indicate that ANG II stimulates Akt/PKB activity via AT1 receptors in VSMC and that the activities of tyrosine kinase and PI3K are required for this activation.

Implication of multiple signaling pathways in the regulation of angiotensin II induced enhanced expression of Giα proteins in vascular smooth muscle cells

2012 ◽  
Vol 90 (8) ◽  
pp. 1105-1116 ◽  
Author(s):  
Yuan Li ◽  
Madhu B. Anand-Srivastava
Keyword(s):  
Smooth Muscle ◽  
Angiotensin Ii ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Receptor Antagonist ◽  
Signaling Pathways ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Ang Ii ◽  
Enhanced Expression

We have previously shown that A10 vascular smooth muscle cells (VSMC) exposed to angiotensin II (Ang II) exhibited overexpression of Giα proteins. In the present study, we examined the involvement of different signaling pathways in regulating Ang II induced enhanced expression of Giα proteins in VSMC by using pharmacological inhibitors. Ang II induced increased expression of Giα proteins in A10 VSMC was markedly attenuated by actinomycin D, losartan (an AT1 receptor antagonist), dibutyryl cAMP, phospholipase C (PLC) inhibitor U73122, protein kinase C (PKC) inhibitors staurosporine and GP109203X, but not by PD123319 (an AT2 receptor antagonist). In addition, BAPTA-AM and TMB-8 (chelators of intracellular Ca2+); and nifedipine (a blocker of L-type Ca2+ channels) significantly inhibited Ang II induced enhanced expression of Giα proteins. On the other hand, extracellular Ca2+ chelation using EGTA did not affect the Ang II evoked enhanced levels of Giα proteins. Furthermore, pretreatment of A10 VSMC with calmidazolium (an inhibitor of calmodulin), or KN93 (an inhibitor of CaM kinase), or genistein (an inhibitor of protein tyrosine kinase, PTK), also attenuated the increased levels of Giα proteins induced by Ang II. These results suggest that Ang II induced enhanced expression of Giα proteins may be regulated by different signaling pathways through AT1 receptors in A10 VSMC.

scholarly journals Deletion of Jak2 Tyrosine Kinase within Vascular Smooth Muscle Cells Attenuates Angiotensin II induced Hypertension in Mice

2010 ◽  
Vol 24 (S1) ◽  
Author(s):  
Annet Kirabo ◽  
Patrick Kearns ◽  
Jennifer Sasser ◽  
Arturo J Cardounel ◽  
Chris Baylis ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Angiotensin Ii ◽  
Vascular Smooth Muscle ◽  
Tyrosine Kinase ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Induced Hypertension
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