scholarly journals Fluid Movement in Occluded Single Capillaries of Rabbit Omentum

1971 ◽  
Vol 28 (3) ◽  
pp. 358-366 ◽  
Author(s):  
J. S. LEE ◽  
L. H. SMAJE ◽  
B. W. ZWEIFACH
Keyword(s):  
Cancers ◽  
2021 ◽  
Vol 13 (13) ◽  
pp. 3128
Author(s):  
Thomas R. Coughlin ◽  
Ali Sana ◽  
Kevin Voss ◽  
Abhilash Gadi ◽  
Upal Basu-Roy ◽  
...  

Osteosarcoma (OS) is an aggressive bone cancer originating in the mesenchymal lineage. Prognosis for metastatic disease is poor, with a mortality rate of approximately 40%; OS is an aggressive disease for which new treatments are needed. All bone cells are sensitive to their mechanical/physical surroundings and changes in these surroundings can affect their behavior. However, it is not well understood how OS cells specifically respond to fluid movement, or substrate stiffness—two stimuli of relevance in the tumor microenvironment. We used cells from spontaneous OS tumors in a mouse engineered to have a bone-specific knockout of pRb-1 and p53 in the osteoblast lineage. We silenced Sox2 (which regulates YAP) and tested the effect of fluid flow shear stress (FFSS) and substrate stiffness on YAP expression/activity—which was significantly reduced by loss of Sox2, but that effect was reversed by FFSS but not by substrate stiffness. Osteogenic gene expression was also reduced in the absence of Sox2 but again this was reversed by FFSS and remained largely unaffected by substrate stiffness. Thus we described the effect of two distinct stimuli on the mechanosensory and osteogenic profiles of OS cells. Taken together, these data suggest that modulation of fluid movement through, or stiffness levels within, OS tumors could represent a novel consideration in the development of new treatments to prevent their progression.


1989 ◽  
pp. 203-217
Author(s):  
Perry L. Blackshear ◽  
Gertrude L. Blackshear ◽  
Paul F. Emerson

1988 ◽  
Vol 33 (9) ◽  
pp. 1153-1158 ◽  
Author(s):  
Ulrich M. Farack ◽  
Rupert Gerzer ◽  
Therese M. Keravis ◽  
Klaus Loeschke

1971 ◽  
Vol 11 (01) ◽  
pp. 57-62
Author(s):  
C.R. Kyle ◽  
R.L. Perrine

Abstract This paper reports on a simple theoretical analysis of dispersion in rapid flow through porous materials, giving a comparison of predicted results with experiments. The analytical model considers a pore structure which acts like a sequence of mixing cells, each coupled with a stagnant zone. Computed results compare very favorably with experimental observations on flow through a staggered matrix of cylinders. This, in turn, has been shown to behave the packed beds of spheres with corresponding properties. Agreement requires that values for certain theoretical parameters be fitted from the data The values required for these parameters are very reasonable. Development of parameters are very reasonable. Development of this approach could be useful for a number of related problems. Introduction The dispersion of two dynamically similar miscible liquids in laminar or turbulent flow through a porous material is a very complex process. However, it can be broken down into four process. However, it can be broken down into four basic mixing mechanisms:Molecular diffusion. Where the flow velocity is appreciable, or pore size is larger, diffusion is usually negligible. Molecular diffusion will not be discussed in this paper.Uneven fluid movement due to irregular pore geometry and inhomogeneities in the media. Both of these factors are difficult to treat, and are usually neglected in theoretical analysis.Uneven fluid movement due to velocity differences within the pores and passages. The zero-velocity boundary condition on each solid surface assures this type of mixing in both laminar and turbulent flow.Mixing by rotational flow, or by turbulent eddies within the pores or passages. The last two are both convective mixing processes and depend primarily upon the level of processes and depend primarily upon the level of energy dissipation in the media, as well as on the geometry of the system. In general as the velocity increases and the friction losses rise, so does the efficiency of the mixing process. Dispersion has been reviewed thoroughly by Perkins and Johnston and has been studied Perkins and Johnston and has been studied extensively by others. DIFFUSION MODEL OF DISPERSION The most commonly used mathematical model for dispersion in both laminar and turbulent flow is a diffusion-type equation (Refs. 1 or 5). The solution for a step function input with flow in the x-direction only, and with negligible lateral gradients, shows that an initial sharp interface degenerates into a broad mixing zone which grows approximately as the square root of the distance traveled. The solution also predicts a normal distribution for concentration as a function of distance. However, in most real systems "tailing" occurs, causing a skewed distribution. Usually the deviation is not serious and the diffusion equation may be used as a good approximation for the actual process. process. DISPERSION IN A TUBE Another simple model for laminar dispersion, neglecting molecular diffusion, is to consider a porous material as a bundle of capillary tubes. porous material as a bundle of capillary tubes. Sir Geoffrey Taylor showed that if one fluid in a capillary tube is displaced by another dynamically similar miscible fluid, the average concentration, C, at the tube exit is given by: 2C = (V /2V)p SPEJ P. 57


1982 ◽  
Vol 243 (3) ◽  
pp. R416-R423 ◽  
Author(s):  
R. N. Barton ◽  
B. J. Passingham

There is evidence for a physiological role of the adrenal cortex in the early responses to limb ischemia in the rat. Trilostane, which inhibits steroid production and prevents the usual rise in corticosterone concentration, impairs compensatory fluid movement during the 3 h after removal of bilateral hindlimb tourniquets and truncates the accompanying hyperglycemia. We have now studied whether altering the corticosterone concentration has similar effects over a 3-h period after a 35% hemorrhage in the conscious rat. After hemorrhage there was only a small rise in plasma glucose concentration, which was unaffected by inhibition of the adrenocortical response with trilostane or its prolongation with adrenocorticotrophic hormone. However, if hindlimb tourniquets were applied 4 h beforehand, the hyperglycemia after hemorrhage was as large as after tourniquet removal and was similarly curtailed by trilostane. Compensatory fluid movement, in contrast, was unaffected by any of the alterations in corticosterone concentration, with or without tourniquets. Thus the method of producing fluid loss is critical in determining whether glucocorticoids play a role in compensation but not in maintaining hyperglycemia after injury.


1975 ◽  
Vol 228 (1) ◽  
pp. 134-140 ◽  
Author(s):  
SM Rosenthal ◽  
LA LaJohn

Swellings of the mouse tail and ear were produced by subjecting them to subatmospheric pressures of minus 40 to minus 80 mmHg for 15-60 min. Increase in volume was measured volumetrically in the tail and gravimetrically in the ear. Blood volume increases in the tail, as measured with 51Cr erythrocytes, contributed a minor part of the fluid increase. Comparison of mice from 3 to 36 wk in age showed a large decrease of fluid movement with age, with major changes during the growth period. Study of permeability of the ear under decreased pressure,to intravenously administred Evans blue, showed no influence of age on permeability to the protein-bound dye. Measurement of transmission of the applied negative pressure through the skin, and of compliance of the tissues of the ear and tail in mice of different age groups, indicated that these factors were not responsible for the observed changes with age.


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