scholarly journals Verification of the Canadian Laboratory Initiative on Paediatric Reference Intervals (CALIPER) reference values in Croatian children and adolescents

2020 ◽  
Vol 30 (2) ◽  
pp. 307-314
Author(s):  
Renata Zrinski Topić ◽  
Jasna Leniček Krleža
Keyword(s):  
Children And Adolescents ◽  
Follicle Stimulating Hormone ◽  
Reference Interval ◽  
Reference Intervals ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Total Testosterone ◽  
Healthy Children ◽  
Hormone Binding ◽  
Stimulating Hormone

Introduction: The aim of this study was to examine whether the Canadian Laboratory Initiative on Paediatric Reference Intervals (CALIPER) could be applied to Croatian children and adolescents. Materials and methods: A total of 295 outpatient healthy children and adolescents of age 1 to 18 were selected using the direct a posteriori sampling method. According to current guidelines, 20 samples were tested for each of a total of 51 reference intervals for ferritin, cortisol, dehydroepiandrosterone sulfate, follicle stimulating hormone, lutein stimulating hormone, prolactin, progesterone, sex hormone binding globulin, thyroid stimulating hormone, total testosterone, total thyroxine and total triiodothyronine. Serum samples were analysed on the Beckman Coulter DxI600 immunoassay analyser by chemiluminescence immunoassay method. A reference interval was adopted if < 10% of the results fall outside CALIPER reference interval range. For analytes in which this criterion is not met in the first set of samples, a new set of 20 samples were collected. Results: After the first set of measurements, 96% of all tested reference intervals were adopted for use. The additional sets of 20 reference subjects were tested for only two reference intervals; follicle stimulating hormone for female aged 1 to 9 years, and irrespective of the gender, sex hormone binding globulin for children aged 8 to 11 years. All results of additional samples were within the specified interval limits. Conclusions: CALIPER reference intervals for ferritin and 11 hormones defined for Beckman Coulter DxI600 immunoassay analyser can be implemented into the Croatian laboratories and clinical practice.

scholarly journals Statistical Genomic Approach Identifies Association between FSHR Polymorphisms and Polycystic Ovary Morphology in Women with Polycystic Ovary Syndrome

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Tao Du ◽  
Yu Duan ◽  
Kaiwen Li ◽  
Xiaomiao Zhao ◽  
Renmin Ni ◽  
...  
Keyword(s):  
Polycystic Ovary ◽  
Follicle Stimulating Hormone ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Nucleotide Polymorphisms ◽  
Hormone Binding ◽  
Functional Variants ◽  
Fshr Gene ◽  
Genomic Approach ◽  
Stimulating Hormone

Background.Single-nucleotide polymorphisms (SNPs) in the follicle stimulating hormone receptor (FSHR) gene are associated with PCOS. However, their relationship to the polycystic ovary (PCO) morphology remains unknown. This study aimed to investigate whether PCOS related SNPs in the FSHR gene are associated with PCO in women with PCOS.Methods. Patients were grouped into PCO (n=384) and non-PCO (n=63) groups. Genomic genotypes were profiled using Affymetrix human genome SNP chip 6. Two polymorphisms (rs2268361 and rs2349415) of FSHR were analyzed using a statistical approach.Results. Significant differences were found in the allele distributions of the GG genotype of rs2268361 between the PCO and non-PCO groups (27.6% GG, 53.4% GA, and 19.0% AA versus 33.3% GG, 36.5% GA, and 30.2% AA), while no significant differences were found in the allele distributions of the GG genotype of rs2349415. When rs2268361 was considered, there were statistically significant differences of serum follicle stimulating hormone, estradiol, and sex hormone binding globulin between genotypes in the PCO group. In case of the rs2349415 SNP, only serum sex hormone binding globulin was statistically different between genotypes in the PCO group.Conclusions. Functional variants in FSHR gene may contribute to PCO susceptibility in women with PCOS.

Effects of gonadotropin-releasing hormone on bioactivity of follicle-stimulating hormone (FSH) and microstructure of FSH, luteinizing hormone and sex hormone-binding globulin in a testosterone-based contraceptive trial: evaluation of responders and non-responders

1996 ◽  
Vol 135 (4) ◽  
pp. 433-439 ◽  
Author(s):  
Manuela Simoni ◽  
Jörg Peters ◽  
Hermann M Behre ◽  
Sabine Kliesch ◽  
Eckhard Leifke ◽  
...  
Keyword(s):  
Luteinizing Hormone ◽  
Concanavalin A ◽  
Follicle Stimulating Hormone ◽  
Gonadotropin Releasing Hormone ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Molecular Heterogeneity ◽  
Hormone Binding ◽  
Releasing Hormone ◽  
Stimulating Hormone

Simoni M, Peters J, Behre HM, Kliesch S, Leifke E, Nieschlag E. Effects of gonadotropin-releasing hormone on bioactivity of follicle-stimulating hormone (FSH) and microstructure of FSH. luteinizing hormone and sex hormone-binding globulin in a testosterone-based contraceptive trial: evaluation of responders and non-responders. Eur J Endocrinol 1996;135:433–9. ISSN 0804–4643 Only a proportion of normal men participating in testosterone-based contraceptive trials develop azoospermia (responders). This study analyzed whether serum follicle-stimulating hormone (FSH), luteinizing hormone (LH) and sex hormone-binding globulin (SHBG) are qualitatively different between responders and non-responders. Determination of in vitro bioactive FSH after stimulation with gonadotropin-releasing hormone (GnRH) and analysis of molecular heterogeneity of serum FSH. LH and SHBG was carried out by chromatofocusing and concanavalin-A affinity chromatography in eight men who had participated in a previous contraceptive study with testosterone buciclate. Blood was withdrawn at 15-min intervals on two basal occasions and 30, 45 and 60 min after iv administration of GnRH (100 μg). Pools of sera were separated by chromatofocusing in the pH range 3–6 and by lectin chromatography on concanavalin A. Immunoreactive FSH, LH and SHBG were assayed in the eluates. Bioactive FSH was analyzed by the rat Sertoli cell bioassay. Serum bioactive FSH increased after GnRH stimulation, without significant differences between responders and non-responders. The chromatofocusing profiles of serum FSH showed a significant shift towards the less acidic region after GnRH. The isoform distribution was similar in responders and non-responders. No significant differences were found in the relative proportion of FSH, LH and SHBG retained by concanavalin A. It is concluded that the extent of suppression of sperm production by androgen administration cannot be foreseen either on the basis of the response of bioactive FSH to GnRH administration or from the glycosylation pattern of serum FSH, LH and SHBG. E Nieschlag, Institute of Reproductive Medicine of the University, Domagkstr. 11, D-48129 Münster, Germany

Biochemical reference intervals for sex hormones with a new AutoDelfia method in aged men

2007 ◽  
Vol 45 (2) ◽  
Author(s):  
Seija Eskelinen ◽  
Tero Vahlberg ◽  
Raimo Isoaho ◽  
Sirkka-Liisa Kivelä ◽  
Kerttu Irjala
Keyword(s):  
Sex Hormones ◽  
Follicle Stimulating Hormone ◽  
Age Groups ◽  
Free Testosterone ◽  
Reference Population ◽  
Reference Intervals ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Reference Ranges ◽  
Hormone Binding

AbstractOur aim was to establish sex hormone reference intervals measured with a new AutoDelfia immunoassay method for aged men free of medication and/or conditions known to influence sex hormone levels.The reference population consisted of 466 individuals between 64 and 97 years (mean 72 years) and a mean body mass index (BMI) of 26.9 kg/mBecause age correlated significantly with most sex hormones studied, we calculated reference intervals for three age groups (64–69, 70–74 and ≥75 years). In clinical practice, single ranges can be used for men aged 64 years or over for testosterone, estradiol and follicle-stimulating hormone (FSH) with the AutoDelfia method. For free testosterone and luteinizing hormone (LH), separate reference intervals should be used for men aged 64–74 years and those aged 75 years or over. For sex hormone-binding globulin, two separate reference intervals by age (64–69 and ≥70 years) are also needed for aged men. LH and FSH reference ranges should be judged with caution, because they may be too high due to cases of subclinical hypogonadism included in the reference population.Clin Chem Lab Med 2007;45:249–53.

scholarly journals Prevalence, Pattern and Predictors for Dyslipidemia of Chinese Women With Polycystic Ovary Syndrome

2021 ◽  
Vol 8 ◽  
Author(s):  
Xi Luo ◽  
Wang-Yu Cai ◽  
Xiao-Ke Wu
Keyword(s):  
Waist Circumference ◽  
Chinese Women ◽  
Polycystic Ovary ◽  
Follicle Stimulating Hormone ◽  
Density Lipoprotein ◽  
Sex Hormone ◽  
Sex Hormone Binding Globulin ◽  
Hormone Binding ◽  
Ovary Syndrome ◽  
Stimulating Hormone

Objective: To investigate the prevalence, pattern and risk predictors for dyslipidemia among Chinese women with polycystic ovary syndrome (PCOS).Study Design and Methods: A total of 1,000 women diagnosed as PCOS by modified Rotterdam criteria were enrolled in 27 hospitals across China in a randomized controlled trial. Anthropometric, metabolic parameters, sex hormone, and lipid levels were measured at the baseline visit. Dyslipidemia was defined according to total cholesterol (TC), low density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), and triglycerides (TG) level. Independent t-test and logistic regression were used to identify predictors for dyslipidemia. Area under the receiver operating characteristic curve (AUC) was calculated.Results: A total of 41.3% of the women had dyslipidemia, and the prevalence of abnormal TC, LDL-C, HDL-C, and TG were 8.6, 9.1, 26.9, and 17.5%, respectively. Logistic regression found that age, waist circumference, insulin, follicle-stimulating hormone, and sex hormone-binding globulin were independent predictors for dyslipidemia. When combining these predictors, the AUC was 0.744. The cut-off points were age &gt;28.5 years, waist circumference &gt;86.5 cm, insulin &gt;96.0 pmol/L, follicle-stimulating hormone &lt;5.6 mIU/mL, and sex hormone-binding hormone &lt;31.0 nmol/L, respectively.Conclusion: Dyslipidemia was common in Chinese women with PCOS, and low HDL-C level was the predominant lipid abnormality. Age, waist circumference, follicle-stimulating hormone, insulin and sex hormone-binding globulin were predictive for dyslipidemia among Chinese women with PCOS.

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