Effects of lipid enrichment of Artemia nauplii for feeding of spiny lebbeid shrimp, Lebbeus groenlandicus (Fabricius, 1775), zoea larvae

Crustaceana ◽  
2016 ◽  
Vol 89 (10) ◽  
pp. 1125-1134
Author(s):  
Kie-Young Park ◽  
Heum-Gi Park ◽  
Woo-Suck Hong ◽  
O-Nam Kwon

This study was conducted to determine the optimum feed selection for the spiny lebbeid shrimp, Lebbeus groenlandicus, from zoea to megalopa larva. To the shrimp zoeae, untreated hatching Artemia meta-nauplii, or either of two kinds of enriched Artemia, were supplied as feed daily, in a concentration of 3 individuals/ml. Throughout the experimental trials, 17.6-18.1 days elapsed until reaching the megalopa larval stage. In the starving trials, no mortality was observed; the mortality values observed in the experimental trials, between 4.8 and 9.5% were considered low and not significantly different (). Neither fatty nor amino acids varied significantly in the zoea I and II stages in either the feeding or starving trials. Highly unsaturated fatty acids and amino acids were significantly lower in the megalopa stage in the starving group, compared with the feeding groups (). Therefore, feeding was proven not to have any effect in the zoea I and II larval stages. However, for the megalopa larval stage, a feed containing supplementary amino acids and fatty acids evoked a positive effect.

2002 ◽  
Vol 364 (1) ◽  
pp. 49-55 ◽  
Author(s):  
Sabine D'ANDREA ◽  
Hervé GUILLOU ◽  
Sophie JAN ◽  
Daniel CATHELINE ◽  
Jean-Noël THIBAULT ◽  
...  

The recently cloned Δ6-desaturase is known to catalyse the first step in very-long-chain polyunsaturated fatty acid biosynthesis, i.e. the desaturation of linoleic and α-linolenic acids. The hypothesis that this enzyme could also catalyse the terminal desaturation step, i.e. the desaturation of 24-carbon highly unsaturated fatty acids, has never been elucidated. To test this hypothesis, the activity of rat Δ6-desaturase expressed in COS-7 cells was investigated. Recombinant Δ6-desaturase expression was analysed by Western blot, revealing a single band at 45kDa. The putative involvement of this enzyme in the Δ6-desaturation of C24:5n-3 to C24:6n-3 was measured by incubating transfected cells with C22:5n-3. Whereas both transfected and non-transfected COS-7 cells were able to synthesize C24:5n-3 by elongation of C22:5n-3, only cells expressing Δ6-desaturase were also able to produce C24:6n-3. In addition, Δ6-desaturation of [1-14C]C24:5n-3 was assayed invitro in homogenates from COS-7 cells expressing Δ6-desaturase or not, showing that Δ6-desaturase catalyses the conversion of C24:5n-3 to C24:6n-3. Evidence is therefore presented that the same rat Δ6-desaturase catalyses not only the conversion of C18:3n-3 to C18:4n-3, but also the conversion of C24:5n-3 to C24:6n-3. A similar mechanism in the n-6 series is strongly suggested.


1995 ◽  
Vol 61 (5) ◽  
pp. 860-866 ◽  
Author(s):  
Pairat Kosutarak ◽  
Akio Kanazawa ◽  
Shin-ichi Teshima ◽  
Shunsuke Koshio

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