Description of Aulolaimus multipapillatus sp. n. and A. nannocephalus Andrássy, 1972 with notes on taxonomy and phylogeny of the genus (Nematoda: Aulolaimidae)

Nematology ◽  
2007 ◽  
Vol 9 (2) ◽  
pp. 201-214 ◽  
Author(s):  
Andrij Susulovsky ◽  
Sven Boström ◽  
Oleksandr Holovachov

AbstractOne new and one known species of Aulolaimus are described, viz. A. multipapillatus sp. n. from Israel and A. nannocephalus from Ukraine. Aulolaimus multipapillatus sp. n. is particularly characterised by a cuticle bearing ca 60 distinct longitudinal ridges, cuticle with distinct ornamentation on ventral body side anterior and posterior to vulva, 6-11 midventral sensilla anterior to cloaca, and spicules ca 25 μm long. Aulolaimus nannocephalus is redescribed on the base of both light and scanning electron microscopic observations on two populations from Ukraine. An emended diagnosis and a revised classification of the genus are proposed. The morphological variability and systematic position of Aulolaimus are discussed and a key to the species of the genus is provided.

2010 ◽  
Vol 6 ◽  
pp. 106-108
Author(s):  
Kunjani Joshi

Since more than two decades, the taxonomy of Hopea has been a point for discussion. Time to time, various taxonomists have tried to classify the species of Hopea on the basis of morphological and anatomical characters. This type of controversy can be solved by the study of various disciplines. Among them, the Scanning Electron Microscopic (SEM) study may be taxonomically useful to resolve the controversies over the classification of the genus Hopea. The SEM study was carried out in Hopea of Sri Lanka. The result of SEM indicates that the two taxa (Hopea jucunda ssp. jucunda and H. jucunda ssp. modesta) possess different epicuticular appearance justifying to upgrade the subspecies of H. jucunda to species level. Key words: epicuticular wax; SEM; stomata; trichome.DOI: 10.3126/botor.v6i0.2919 Botanica Orientalis - Journal of Plant Science (2009) 6: 106-108


Author(s):  
Toichiro Kuwabara

Although scanning electron microscopy has a great potential in biological application, there are certain limitations in visualization of the biological structure. Satisfactory techniques to demonstrate natural surfaces of the tissue and the cell have been reported by several investigators. However, it is commonly found that the surface cell membrane is covered with a minute amount of mucin, secretory substance or tissue fluid as physiological, pathological or artefactual condition. These substances give a false surface appearance, especially when the tissue is fixed with strong fixatives. It seems important to remove these coating substances from the surface of the cell for demonstration of the true structure.


Author(s):  
T. Kanetaka ◽  
M. Cho ◽  
S. Kawamura ◽  
T. Sado ◽  
K. Hara

The authors have investigated the dissolution process of human cholesterol gallstones using a scanning electron microscope(SEM). This study was carried out by comparing control gallstones incubated in beagle bile with gallstones obtained from patients who were treated with chenodeoxycholic acid(CDCA).The cholesterol gallstones for this study were obtained from 14 patients. Three control patients were treated without CDCA and eleven patients were treated with CDCA 300-600 mg/day for periods ranging from four to twenty five months. It was confirmed through chemical analysis that these gallstones contained more than 80% cholesterol in both the outer surface and the core.The specimen were obtained from the outer surface and the core of the gallstones. Each specimen was attached to alminum sheet and coated with carbon to 100Å thickness. The SEM observation was made by Hitachi S-550 with 20 kV acceleration voltage and with 60-20, 000X magnification.


Author(s):  
Loren Anderson ◽  
Pat Pizzo ◽  
Glen Haydon

Transmission electron microscopy of replicas has long been used to study the fracture surfaces of components which fail in service. Recently, the scanning electron microscope (SEM) has gained popularity because it allows direct examination of the fracture surface. However, the somewhat lower resolution of the SEM coupled with a restriction on the sample size has served to limit the use of this instrument in investigating in-service failures. It is the intent of this paper to show that scanning electron microscopic examination of conventional negative replicas can be a convenient and reliable technique for determining mode of failure.


Author(s):  
Peter M. Andrews

Although there have been a number of recent scanning electron microscopic reports on the renal glomerulus, the advantages of scanning electron microscopy have not yet been applied to a systematic study of the uriniferous tubules. In the present investigation, scanning electron microscopy was used to study the ultrastructural morphology of the proximal, distal, thin loop, and collecting tubules. Material for observation was taken from rat kidneys which were fixed by vascular perfusion, sectioned by either cutting or fracturing technigues, and critically point dried.The brush border characterising proximal tubules is first detected on the luminal surface of Bowman's capsule adjacent to the urinary pole orifice. In this region one frequently finds irregular microvilli characterized by broad and flattened bases with occasional bulbous structures protruding from their surfaces.


Author(s):  
M.D. Graham

The recent development of the scanning electron microscope has added great impetus to the study of ultrastructural details of normal human ossicles. A thorough description of the ultrastructure of the human ossicles is required in order to determine changes associated with disease processes following medical or surgical treatment.Human stapes crura were obtained at the time of surgery for clinical otosclerosis and from human cadaver material. The specimens to be examined by the scanning electron microscope were fixed immediately in the operating room in a cold phosphate buffered 2% gluteraldehyde solution, washed with Ringers, post fixed in cold 1% osmic acid and dehydrated in graded alcohol. Specimens were transferred from alcohol to a series of increasing concentrations of ethyl alcohol and amyl acetate. The tissue was then critical point dried, secured to aluminum stubs and coated with gold, approximately 150A thick on a rotating stage in a vacuum evaporator. The specimens were then studied with the Kent-Cambridge S4-10 Scanning Electron Microscope at an accelerating voltage of 20KV.


Author(s):  
A. E. Hotchkiss ◽  
A. T. Hotchkiss ◽  
R. P. Apkarian

Multicellular green algae may be an ancestral form of the vascular plants. These algae exhibit cell wall structure, chlorophyll pigmentation, and physiological processes similar to those of higher plants. The presence of a vascular system which provides water, minerals, and nutrients to remote tissues in higher plants was believed unnecessary for the algae. Among the green algae, the Chaetophorales are complex highly branched forms that might require some means of nutrient transport. The Chaetophorales do possess apical meristematic groups of cells that have growth orientations suggestive of stem and root positions. Branches of Chaetophora incressata were examined by the scanning electron microscope (SEM) for ultrastructural evidence of pro-vascular transport.


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