Scanning electron microscopic study of leaf surface of some species of Hopea Roxb. (Dipterocarpaceae): implications for taxonomy

Sem Study ◽

Anatomical Characters ◽

Since more than two decades, the taxonomy of Hopea has been a point for discussion. Time to time, various taxonomists have tried to classify the species of Hopea on the basis of morphological and anatomical characters. This type of controversy can be solved by the study of various disciplines. Among them, the Scanning Electron Microscopic (SEM) study may be taxonomically useful to resolve the controversies over the classification of the genus Hopea. The SEM study was carried out in Hopea of Sri Lanka. The result of SEM indicates that the two taxa (Hopea jucunda ssp. jucunda and H. jucunda ssp. modesta) possess different epicuticular appearance justifying to upgrade the subspecies of H. jucunda to species level. Key words: epicuticular wax; SEM; stomata; trichome.DOI: 10.3126/botor.v6i0.2919 Botanica Orientalis - Journal of Plant Science (2009) 6: 106-108

Scanning Electron Microscopic Study of the Eggs of the Warthog Louse (Haematopinus Phacochoeri)

Microscopy and Microanalysis ◽

10.1017/s1431927600007455 ◽

1997 ◽

Vol 3 (S2) ◽

pp. 113-114

Author(s):

C. Baker ◽

E.D. Green

Keyword(s):

Electron Microscope ◽

Scanning Electron Microscope ◽

Environmental Changes ◽

General Structure ◽

Electron Microscopic ◽

Razor Blade ◽

Sem Study ◽

The large eggs of Haematopinus phacochoeri are found attached to the long dorsal mane bristles of the diurnal warthog. The eggs are generally exposed to great environmental stresses such as excessive sunlight and temperature as well as the occasional plunge under water or mud during grooming. This investigation was therefore undertaken to determine how the eggs of H. phococoeri are adapted to tolerate the various environmental changes it is constantly exposed to. To our knowledge no other SEM study on the eggs of this species has been performed.Specimens still attached to the bristles were fixed in 70% ethanol, dehydrated and ultrasonically cleaned. Some eggs were longitudinally sectioned with a razor blade to expose the chorionic layers. The eggs were critical point dried in liquid CO2, mounted and viewed by means of a Leica Stereoscan 420 scanning electron microscope.The general structure of the shell consists of two layers of chorion, i.e. the endo-and exochorion. These two layers are attached at the pre-formed line of weakness at the operculum and again near the hydropyle. Elsewhere these two layers are completely separated by the respiratory layer.

A Scanning Electron Microscopic Study of Changes in Microtubule Distributions During Secondary Wall Formation in Tracheids

IAWA Journal ◽

10.1163/22941932-90001360 ◽

1994 ◽

Vol 15 (2) ◽

pp. 185-189 ◽

Cited By ~ 9

Author(s):

Hisashi Abe ◽

Jun Ohtani ◽

Kazumi Fukazawa

Keyword(s):

Secondary Wall ◽

Secondary Cell Wall ◽

Electron Microscopic ◽

Secondary Cell Wall Formation ◽

Wall Formation ◽

Sem Study ◽

Secondary Wall Formation ◽

A quantitative scanning electron microscopic (SEM) study of the changes in microtubule orientations and arrays during secondary wall formation has been done on conifer (Abies sachalinensis Masters) tracheids. Microtubules have similar orientations as the microfibrils being deposited in the various wall layers. The density of microtubules is different in different stages of secondary cell wall formation. Microtubules are more closely arrayed in the tracheids forming the S2 than the S12 and S3. During S3 formation, sometimes 2-7 microtubules are closely arrayed, and form bundles about 80-350 nm wide. Bundles of microfibrils of similar width were also observed during S3 formation.

Structural and textural classification of erythrocytes in anaemic cases: A scanning electron microscopic study

Micron ◽

10.1016/j.micron.2012.09.003 ◽

2013 ◽

Vol 44 ◽

pp. 384-394 ◽

Cited By ~ 10

Author(s):

Sirsendu Bhowmick ◽

Dev Kumar Das ◽

Asok Kumar Maiti ◽

Chandan Chakraborty

Keyword(s):

Microscopic Study ◽

Electron Microscopic Study ◽

Electron Microscopic ◽

Scanning Electron Microscopic Study of Surface Epithelial Cells in Erosive and Nonerosive Oral Lichen Planus

The Journal of Contemporary Dental Practice ◽

10.5005/jp-journals-10024-1077 ◽

2011 ◽

Vol 12 (6) ◽

pp. 463-468 ◽

Cited By ~ 2

Author(s):

Manjiri Thakur ◽

Vinay Hazare

Keyword(s):

Epithelial Cells ◽

Lichen Planus ◽

Oral Lichen Planus ◽

Normal Mucosa ◽

Electron Microscopic ◽

Sem Study ◽

Oral Lichen ◽

ABSTRACT Aim Aim of the study is to study the surface epithelial cells of erosive, nonerosive lichen planus by scanning electron microscope (SEM) and to correlate it with that of normal mucosa and oral leukoplakia. Materials and methods Twenty biopsies of lichen planus and five biopsies of normal mucosa were taken. Half piece of each biopsy was processed for H&E stain and another half for SEM study. Result Surface epithelial cells were showing different features. Conclusion Definite different features were seen in lichen planus-leukoplakia group than in normal oral mucosa. These changes are due to abnormality in cell maturation and differentiation. Clinical significance SEM study is helpful in early detection of dysplasia. It can lead to early treatment of the lesion and thus prevention of squamous cell carcinoma. How to cite this article Thakur M, Hazare V. Scanning Electron Microscopic Study of Surface Epithelial Cells in Erosive and Nonerosive Oral Lichen Planus. J Contemp Dent Pract 2011; 12(6):463-468.

Scanning Electron Microscopic Study of the Cell Surface

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100062087 ◽

1969 ◽

Vol 27 ◽

pp. 36-37 ◽

Cited By ~ 1

Author(s):

Toichiro Kuwabara

Keyword(s):

Tissue Fluid ◽

Biological Application ◽

Biological Structure ◽

Electron Microscopic ◽

Surface Appearance ◽

Minute Amount ◽

True Structure ◽

Although scanning electron microscopy has a great potential in biological application, there are certain limitations in visualization of the biological structure. Satisfactory techniques to demonstrate natural surfaces of the tissue and the cell have been reported by several investigators. However, it is commonly found that the surface cell membrane is covered with a minute amount of mucin, secretory substance or tissue fluid as physiological, pathological or artefactual condition. These substances give a false surface appearance, especially when the tissue is fixed with strong fixatives. It seems important to remove these coating substances from the surface of the cell for demonstration of the true structure.

A scanning electron microscopic study on dissolving process of cholesterol gallstones by chenodeoxycholic acid (CDCA)

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100083485 ◽

1978 ◽

Vol 36 (2) ◽

pp. 522-523

Author(s):

T. Kanetaka ◽

M. Cho ◽

S. Kawamura ◽

T. Sado ◽

K. Hara

Keyword(s):

Electron Microscope ◽

Chenodeoxycholic Acid ◽

Outer Surface ◽

Electron Microscopic ◽

Cholesterol Gallstones ◽

The Core ◽

Acceleration Voltage ◽

The authors have investigated the dissolution process of human cholesterol gallstones using a scanning electron microscope(SEM). This study was carried out by comparing control gallstones incubated in beagle bile with gallstones obtained from patients who were treated with chenodeoxycholic acid(CDCA).The cholesterol gallstones for this study were obtained from 14 patients. Three control patients were treated without CDCA and eleven patients were treated with CDCA 300-600 mg/day for periods ranging from four to twenty five months. It was confirmed through chemical analysis that these gallstones contained more than 80% cholesterol in both the outer surface and the core.The specimen were obtained from the outer surface and the core of the gallstones. Each specimen was attached to alminum sheet and coated with carbon to 100Å thickness. The SEM observation was made by Hitachi S-550 with 20 kV acceleration voltage and with 60-20, 000X magnification.

A Scanning Electron Microscopic Study of the Uriniferous Tubules

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100073556 ◽

1973 ◽

Vol 31 ◽

pp. 622-623

Author(s):

Peter M. Andrews

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Renal Glomerulus ◽

Electron Microscopic ◽

Vascular Perfusion ◽

Collecting Tubules ◽

Bowman's Capsule ◽

Although there have been a number of recent scanning electron microscopic reports on the renal glomerulus, the advantages of scanning electron microscopy have not yet been applied to a systematic study of the uriniferous tubules. In the present investigation, scanning electron microscopy was used to study the ultrastructural morphology of the proximal, distal, thin loop, and collecting tubules. Material for observation was taken from rat kidneys which were fixed by vascular perfusion, sectioned by either cutting or fracturing technigues, and critically point dried.The brush border characterising proximal tubules is first detected on the luminal surface of Bowman's capsule adjacent to the urinary pole orifice. In this region one frequently finds irregular microvilli characterized by broad and flattened bases with occasional bulbous structures protruding from their surfaces.

A Scanning Electron Microscopic Study of Pro-Vascular Cellular Morphology in Chaetophora Incressata

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100119880 ◽

1985 ◽

Vol 43 ◽

pp. 638-639

Author(s):

A. E. Hotchkiss ◽

A. T. Hotchkiss ◽

R. P. Apkarian

Keyword(s):

Green Algae ◽

Vascular Plants ◽

Vascular System ◽

Higher Plants ◽

Wall Structure ◽

Electron Microscopic ◽

Physiological Processes ◽

Multicellular green algae may be an ancestral form of the vascular plants. These algae exhibit cell wall structure, chlorophyll pigmentation, and physiological processes similar to those of higher plants. The presence of a vascular system which provides water, minerals, and nutrients to remote tissues in higher plants was believed unnecessary for the algae. Among the green algae, the Chaetophorales are complex highly branched forms that might require some means of nutrient transport. The Chaetophorales do possess apical meristematic groups of cells that have growth orientations suggestive of stem and root positions. Branches of Chaetophora incressata were examined by the scanning electron microscope (SEM) for ultrastructural evidence of pro-vascular transport.

Haemostatic Clot Formation at Anastomosis of Synthetic Venous Graft in Defibrinogenated Dogs: A Scanning Electron Microscopic Study

Thrombosis and Haemostasis ◽

10.1055/s-0038-1650187 ◽

1981 ◽

Vol 45 (03) ◽

pp. 276-281 ◽

Cited By ~ 2

Author(s):

S Ishimaru ◽

E Berglin ◽

H-A Hansson ◽

A-C Teger-Nilsson ◽

G William-Olsson

Keyword(s):

Vena Cava ◽

Treated Group ◽

Control Group ◽

Electron Microscopic ◽

Fibrin Network ◽

Expanded Polytetrafluoroethylene Graft ◽

Morphological Differences ◽

SummaryA segment of the inferior vena cava was replaced by an expanded polytetrafluoroethylene graft in 13 dogs. Five of them served as a control group, while the other 8 were moderately or severely defibrinogenated with subcutaneous batroxobin. Plasma fibrinogen decreased to extremely low values throughout the experiment in the defibrinogenated dogs except in the moderately treated group in which it temporarily rose to 0.72-0.87 g/1 on the first postoperative day.Scanning electron microscopic observations of the haemostatic clot formed at the anastomoses of the graft revealed no significant morphological differences in platelet adhesion and/or aggregation between the three groups. These findings confirmed that platelets play a key role in primary haemostasis during defibrinogenation.The fibrin network was slightly diminished and only short fibrin filaments could be seen in the moderately and severely defibrinogenated groups respectively. These differences in composition of the clots are discussed in relation to their haemostatic capacity.