Description of Lelenchus brevislitus n. sp. (Nematoda: Tylenchidae), an example of a cryptic species from Iran and its phylogenetic relationships with other species in the family

Lelenchus brevislitus n. sp. is described and illustrated based on morphological, morphometric and molecular data. The new species is characterised by having females with slender, 452-488 μm long, body lateral field vestigial as a plain simple band under scanning electron microscopy (SEM) in vulval region and absent in proximal and distal body ends, cephalic region short, ca 2 μm high, conical and truncate in anterior end in lateral view under SEM, dorso-ventrally flattened, amphidial pouches pocket-shaped and large under light microscopy (LM), their apertures short (ca 1 μm long in SEM) longitudinal sinuous slits remarkably wide near the small oral disc and significantly narrowing posteriad stylet, slender. 6.5-8.0 μm long, developed median bulb with distinct valvular plates, monodelphic-prodelphic reproductive system having a short post-vulval uterine sac (PUS), transverse vulval slit (V = 47.5-51.7) bearing small lateral membranes on each side, long filiform female tail (154-170 μm, c = 2.8-3.1, c′ = 21.5-28.3), and males unknown. The new species is considered to be a cryptic species inside the Tylenchidae. Morphological comparisons with all species of the genus are discussed. Phylogenetic studies were performed for further characterisation of the new species, and the results, using nearly full length 18S rDNA data, revealed it formed a clade with an isolate of L. leptosoma, both of which are in a sister clade with an isolate of Sakia. In a reconstructed phylogenetic tree using partial 28S rDNA sequences, the new species formed a monophyletic group, again with L. leptosoma, a sister clade to some Malenchus spp.

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Description of Atetylenchus minor n. sp. (Tylenchina: Tylenchidae) and data on two other species of the family

Nematology ◽

10.1163/15685411-00002918 ◽

2015 ◽

Vol 17 (8) ◽

pp. 981-994 ◽

Cited By ~ 7

Author(s):

Ali Yaghoubi ◽

Ebrahim Pourjam ◽

Mohammad Reza Atighi ◽

Majid Pedram

Keyword(s):

New Species ◽

Posterior Probability ◽

Bayesian Posterior Probability ◽

Lateral Field ◽

Phylogenetic Studies ◽

Sister Clade ◽

The Family ◽

Molecular Phylogenetic ◽

Morphological Differences ◽

Short Body

Three species of the family Tylenchidae, including one new and two known species, are described and illustrated. Atetylenchus minor n. sp. is characterised by its short body length of 633 (558-691) μm, MB = 45 (41-46), lateral field with four crenate incisures, stylet 12.0 (11.5-12.5) μm long, without distinct basal knobs but with swollen arms of the shaft at its base, V = 56.3 (53.7-59.3), absence of lateral vulval membranes, and conical tail 57 (51-62) μm long with a rounded tip. Morphological differences of the new species with four known species of the genus, namely A. abulbosus, A. graminus, A. amiri and A. metaporus, are discussed. Molecular phylogenetic studies of the new species using 1089 bp partial sequences of 18S rDNA revealed that the new species formed a clade with an unpublished species assigned to Psilenchus in Bayesian inference (BI) with low Bayesian posterior probability (BPP). This clade forms a sister clade to a major clade containing two species of Psilenchus and several belonolaim species and genera. The same result was achieved using maximum likelihood (ML) analysis. Morphological and molecular phylogenetic studies were also performed on two other species, Malenchus labiatus and Neothada cancellata, using 28S rDNA D2-D3 sequences. In the inferred Bayesian tree, M. labiatus formed a clade with other species of Malenchus with 28S information with maximal support (1.00 BPP), while N. cancellata formed a clade with Boleodorinae, again with maximal (1.00) BPP.

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Description of a new species of the genus Anguillonema Fuchs, 1938 (Nematoda: Sphaerularioidea) with an identification key to the species

Journal of Helminthology ◽

10.1017/s0022149x18000470 ◽

2018 ◽

Vol 93 (04) ◽

pp. 504-512

Author(s):

A. Yaghoubi ◽

E. Pourjam ◽

M. Pedram

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Thin Body ◽

Molecular Characteristics ◽

Lateral Field ◽

Phylogenetic Studies ◽

Sister Clade ◽

Molecular Phylogenetic ◽

Morphological Differences ◽

18S Rdna Sequence

AbstractAnguillonema iranicum n. sp. is described and illustrated as the second species of this genus from Iran, based on morphological, morphometric and molecular characteristics. It is identified by a short, thin body, a continuous lip region, six lines on the lateral field, a short, thin stylet, a posteriorly located pharyngo-intestinal junction to excretory pore, the presence of a post-vulval uterine sac, vulval position at 89% (87.4–89.9%) of body length, an elongate conoid tail with a rounded to pointed tip and not dorsally bent, and common functional males with short spicules and lacking a bursa. Morphological differences between the new species and the three known species of the genus, namely A. amolensis, A. crenati and A. poligraphi, are discussed. Molecular phylogenetic studies of the new species using partial 18S rDNA sequence revealed that it formed a sister clade with three species of Howardula, one species of Anguillonema and one unidentified isolate. In phylogenetic analyses using partial sequences of 28S rDNA D2-D3 segment, the new species formed a clade with two isolates of Parasitylenchus. A key to identification of Anguillonema species is also presented.

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Discovery of a Cryptic Species, Erysiphe Salicina Sp. Nov., and Reconstruction of The Phylogeny of Powdery Mildews On Populus and Salix Spp.

10.21203/rs.3.rs-1199630/v1 ◽

2022 ◽

Author(s):

Jing Feng ◽

Shu-Yan Liu ◽

Uwe Braun ◽

Susumu Takamatsu ◽

Michael Bradshaw ◽

...

Keyword(s):

New Species ◽

Cryptic Species ◽

Internal Transcribed Spacer ◽

Phylogenetic Analyses ◽

Closely Related Species ◽

Rdna Sequences ◽

Powdery Mildews ◽

Salix Gracilistyla ◽

28S Rdna Sequences ◽

Salix Spp

Abstract Recently performed phylogenetic-taxonomic analyses of species belonging to Erysiphe sect. Uncinula on willows (Salix spp.) demonstrated a much higher diversity than previously assumed. Phylogenetic analyses and morphological examinations of Chinese Erysiphe collections on Salix abscondita (= S. raddeana), S. sinica and S. taraikensis, all belonging to Salix subgen. Vetrix sect. Vetrix, revealed an additional cryptic species in sister position to the E. salicis clade, which is described as Erysiphe salicina. The new species clearly distinguished from all allied species in morphological and genetical results. The phylogeny of the new species and closely related species on Populus and Salix spp. (Salicaceae) was reconstructed and discussed using a combined alignment of the internal transcribed spacer (ITS) regions and 28S rDNA sequences. The present phylogenetic analyses suggest that the recently described E. salicicola (on Salix gracilistyla in South Korea) has to be reduced to synonymy with E. salicis var. salicis-gracilistylae (º Uncinula salicis-gracilistylae).

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Description of Pratylenchoides ojcowensis sp. nov. (Nematoda: Merlinidae) from Polish Jurassic Highland

Zootaxa ◽

10.11646/zootaxa.4742.1.7 ◽

2020 ◽

Vol 4742 (1) ◽

pp. 117-128

Author(s):

GRAŻYNA WINISZEWSKA ◽

RENATA DOBOSZ ◽

TADEUSZ MALEWSKI ◽

ANDRZEJ SKWIERCZ

Keyword(s):

New Species ◽

Middle Part ◽

The Body ◽

28S Rdna ◽

Body Contour ◽

Lateral Field ◽

Female Head ◽

Rdna Sequences ◽

The Status ◽

28S Rdna Sequences

A new species of the genus Pratylenchoides has been described. It was found in Polish Jurassic Highland, in Ojców National Park. Pratylenchoides ojcowensis sp. nov. was isolated from the soil located around tangled roots of Elymus sp. and Trifolium sp. This species is marked by a conical head in both females and males which is not separated from the body contour and has with 4–5 annuli; a relatively short stylet (20.3–21.3 µm females, 17.7–20.9 µm males) with oval knobs directed posteriorly; the dorsal pharyngeal nucleus located anterior to the cardia (the subventral pharyngeal nuclei located posterior; a pharyngeal lobe of length about two body widths (1.8–2.6); a lateral field with 6 lines in the middle part of body and sometimes with partially areolated outer bands; intestinal fasciculi present; round sperm in the spermatheca in females; a female tail with a maximum of 29 annuli, and an annulated tail terminus. The status of the new species has been verifiied by DNA sequencing and phylogenetic analysis of the 28S rDNA region. The results obtained in the study indicated that P. ojcowensis sp. nov. is most related to P. alkani, P. ritteri and P. nevadensis from which is distinguished by the shape of the female head (conoid vs rounded), shorter stylet in females (20.3–21.3 µm vs 22.0–25.0 µm, 21.0–25.0 µm, 22.0–26.0 µm) and differences in 28S rDNA sequences. In addition (as per the original descriptions Yüksel 1977, Sher 1970, Talavera & Tobar 1996) it is distinguished from P. alkani by smaller number of male’s head annuli (4–5 vs 7–9), from P. ritteri it is distinguished by posteriorly directed stylet knobs (vs directed laterally), from P. nevadensis it is distinguished by oval and posteriorly directed stylet knobs (vs rounded and directed laterally).

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Heterodera circeae sp. n. and H. scutellariae sp. n. (Tylenchida: Heteroderidae) from Germany, with notes on the goettingiana group

Nematology ◽

10.1163/1568541042360582 ◽

2004 ◽

Vol 6 (3) ◽

pp. 343-355 ◽

Cited By ~ 2

Author(s):

Sergei Subbotin ◽

Dieter Sturhan

Keyword(s):

New Species ◽

Tail Length ◽

Molecular Data ◽

The Other ◽

Phylogenetic Position ◽

Lateral Field ◽

Second Stage ◽

Rdna Sequences ◽

Dicotyledonous Plants ◽

Stylet Length

Abstract Two species of cyst-forming nematodes belonging to the goettingiana group are described from woodland in Germany. Heterodera circeae sp. n., parasitising Circaea lutetiana (Onagraceae), most closely resembles H. carotae and H. cruciferae, from which it differs by larger cysts (430-720 μm) and longer fenestral length (27-54 μm) and rounded stylet knobs of the second-stage juveniles. Heterodera scutellariae sp. n., found on the labiate Scutellaria galericulata (Lamiaceae), is distinguished from the other species of this group by shorter body (358-437 μm) and tail (41-53 μm) of the second-stage juveniles. The ITS-rDNA sequences of the new species are very similar and differ from each other by only a few nucleotides. The phylogenetic position of the new species within the group is presented based on analyses of molecular data. A revised diagnosis of the goettingiana group is proposed. The species of the group have lemon-shaped, ambifenestrate and abullate (small bullae occasionally present) cysts with egg sac, long vulval slit (> 35 μm), thin vulval bridge, low semifenestrae (average fenestral length 30-45 μm), and weak underbridge. Second-stage juveniles are characterised by body length > 400 μm, tail length > 45 μ m, hyaline tail portion > 20 μm, stylet length > 20 μm, lateral field with four incisures and small phasmids. All known species of the goettingiana group exclusively parasitise dicotyledonous plants.

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Neoalbatrellus odorus sp. nov. (Albatrellaceae, Russulales) from Southwest China

Phytotaxa ◽

10.11646/phytotaxa.309.3.2 ◽

2017 ◽

Vol 309 (3) ◽

pp. 217 ◽

Cited By ~ 2

Author(s):

YUAN-YUAN CHEN ◽

MIN WANG ◽

BO ZHANG ◽

BAO-KAI CUI

Keyword(s):

New Species ◽

Bayesian Inference ◽

Maximum Likelihood ◽

Southwest China ◽

Phylogenetic Analyses ◽

Molecular Data ◽

Pore Surface ◽

Rdna Sequences ◽

Dark Violet ◽

28S Rdna Sequences

We describe herein the Neoalbatrellus odorus sp. nov. accounting on its peculiar morphological features and molecular data. It is characterized by fleshy basidiomata with a deep violet to dark violet pileal surface, which turns blackish blue and glossy after drying. It also has a white pore surface, light violet to bluish violet stipe, simple septate generative hyphae, and thick-walled, non-amyloid basidiospores. The phylogenetic analyses, based on ITS and 28S rDNA sequences of Neoalbatrellus and its related genera, were performed using Maximum Parsimony, Maximum Likelihood and Bayesian Inference, confirming the affiliation of the new species to Neoalbatrellus.

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Data on some members of the family Tylenchidae (Nematoda: Tylenchina) from Iran

Biologia ◽

10.1515/biolog-2015-0160 ◽

2015 ◽

Vol 70 (10) ◽

Cited By ~ 5

Author(s):

Yousef Panahandeh ◽

Ebrahim Pourjam ◽

Farzad Aliramaji ◽

Majid Pedram

Keyword(s):

Body Length ◽

Phylogenetic Analyses ◽

Tail Length ◽

Iranian Population ◽

Lateral Field ◽

Rdna Sequences ◽

The Family ◽

Molecular Phylogenetic ◽

28S Rdna Sequences ◽

Stylet Length

AbstractThree species belonging to the family Tylenchidae, Malenchus nanellus, M. undulatus and Tylenchus naranensis, are reported for the first time for Iran. These species are characterized based on morphological and morphometric data. The Iranian population of M. nanellus is characterized by its body length ranging from 410-485 μm, cuticle annuli 1.1- 1.5 μm wide at mid-body and lateral field with two crenate lines, starting at the mid-region of procorpus and ending near 1/3 of tail length. The population of M. undulatus is characterized by its 458-526 μm body length, cuticle coarsely annulated, annuli 1.8-2.4 μm wide at mid-body, lateral field with crenate incisures, beginning at about half of the stylet length, ending at middle of tail, head narrower than adjacent body, median bulb well developed with prominent valve plates and functional males in population. Iranian population of T. naranensis, is characterized by having a 631-774 μm body length, lateral field with four lines, outer lines crenate, a stylet length ranging from 10-11 μm and a tail of 102-131 μm long. Molecular phylogenetic analyses based on partial 28S rDNA sequences of T. naranensis revealed its close affinity with the genus Filenchus. Other Tylenchidae genera, such as Aglenchus (including the newly sequenced isolate from Iran) and Coslenchus were sister taxa and formed a well-supported clade. Malenchus exiguus, a previously reported species from Iran and sequenced in the present study, formed a monophyletic clade with other species of Malenchus and Lelenchus leptosoma.

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A pot-pourri of new species of Trypetheliaceae resulting from molecular phylogenetic studies

The Lichenologist ◽

10.1017/s0024282916000475 ◽

2016 ◽

Vol 48 (6) ◽

pp. 639-660 ◽

Cited By ~ 8

Author(s):

Robert LÜCKING ◽

Matthew P. NELSEN ◽

André APTROOT ◽

Michel N. BENATTI ◽

Nguyen Quoc BINH ◽

...

Keyword(s):

New Species ◽

Molecular Data ◽

Orange Pigment ◽

Tropical Asia ◽

Phylogenetic Studies ◽

The Family ◽

Molecular Phylogenetic ◽

Yellow Orange ◽

Thallus Surface ◽

Its Barcoding

AbstractBased on separately obtained and analyzed molecular data and within the framework of a global revision of the family Trypetheliaceae, 21 new species are described, from the Neotropics and tropical Asia, in the genera Architrypethelium (1), Astrothelium (15), Bathelium (1), Nigrovothelium (1), Trypethelium (1), and Viridothelium (2), namely: Architrypethelium lauropaluanum Lücking, M. P. Nelsen & Marcelli sp. nov., differing from A. hyalinum in the perithecia immersed between coarse thallus verrucae and in the additional ascospore septa; Astrothelium aurantiacocinereum Lücking, Naksuwankul & Lumbsch sp. nov., differing from A. aeneum in the prominent, well-delimited, trypethelioid pseudostromata and the absence of pigment on the thallus surface, as well as in the barely lichenized thallus; A. carassense Lücking, M. P. Nelsen & Marcelli sp. nov., differing from A. purpurascens in orange, K+ red pseudostroma pigment and the slightly larger ascospores; A. cryptolucens Lücking, M. P. Nelsen & N. Salazar sp. nov., differing from A. carrascoense in the inspersed hymenium; A. fijiense Lücking, Naksuwankul & Lumbsch sp. nov., differing from A. cinereorosellum in the presence of lichexanthone on the well-delimited pseudostromata and in the slightly shorter ascospores; A. laevithallinum Lücking, M. P. Nelsen & Marcelli sp. nov., differing from A. endochryseum in the smooth thallus; A. leucosessile Lücking, M. P. Nelsen & Aptroot sp. nov., differing from A. phlyctaena in the conspicuous, sessile pseudostromata; A. macrostomoides Lücking, M. P. Nelsen & Benatti sp. nov., differing from A. macrostomum in the larger ascospores; A. megacrypticum Lücking, M. P. Nelsen & N. Salazar sp. nov., differing from A. longisporum in the single-spored asci and larger ascospores; A. nicaraguense Lücking, M. P. Nelsen & T. Orozco sp. nov., differing from A. gigantosporum in the smaller ascospores; A. norisianum Lücking, M. P. Nelsen & Aptroot sp. nov., differing from A. sepultum in the distinct, well-delimited pseudostromata; A. obtectum Lücking, M. P. Nelsen & Benatti sp. nov., differing from A. nigrocacuminum in the smaller ascospores; A. sordithecium Lücking, M. P. Nelsen & Marcelli sp. nov., differing from A. leucothelium in the inspersed hymenium and the absence of lichexanthone from the thallus surface outside the pseudostromata; A. subendochryseum Lücking, M. P. Nelsen & Marcelli sp. nov., differing from A. endochryseum in the absence of pigment in the pseudostromata and the lateral thallus cover of the pseudostromata; A. subinterjectum Lücking, M. P. Nelsen & Jungbluth sp. nov., differing from A. obtectum in the smaller pseudostromata and smaller ascospores, and from A. interjectum in the diffuse pseudostromata and smaller ascospores; Bathelium porinosporum Lücking, M. P. Nelsen & Gueidan sp. nov., differing from other Bathelium species in the 3-septate, euseptate ascospores; Nigrovothelium bullatum Lücking, Upreti & Lumbsch sp. nov., differing from N. tropicum in the bullate thallus; Trypethelium tolimense Lücking, Moncada & M. Gut. sp. nov., differing from T. xanthoplatystomum in the absence of a yellow-orange pigment on the pseudostromata and the K+ yellow (not K+ red) medullary pigment; Viridothelium tricolor Lücking, M. P. Nelsen & N. Salazar sp. nov., characterized by black perithecia with a lateral ostiole immersed in white pseudostromata strongly contrasting with the surrounding brown thallus, in combination with 2-spored asci and large, muriform ascospores; and V. vonkonratii Lücking, Naksuwankul & Lumbsch sp. nov., differing from V. virens in larger ascospores and mostly solitary ascomata. All species are illustrated and their taxonomy and phylogenetic relationships are discussed. ITS barcoding sequences are reported for five specimens of Bathelium porinosporum.

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Taxonomy and phylogeny of Koinocystididae (Platyhelminthes, Kalyptorhynchia), with the description of three new genera and twelve new species

Zootaxa ◽

10.11646/zootaxa.4948.4.1 ◽

2021 ◽

Vol 4948 (4) ◽

pp. 451-500

Author(s):

YANDER L. DIEZ ◽

MARLIES MONNENS ◽

ROSA ISABEL AGUIRRE ◽

RANA YURDUSEVEN ◽

PHILIPPE JOUK ◽

...

Keyword(s):

New Species ◽

New Caledonia ◽

New Genera ◽

Rdna Sequences ◽

The Family ◽

Molecular Phylogenetic ◽

28S Rdna Sequences ◽

The Common ◽

Hard Structures ◽

New Distribution

The taxon Koinocystididae is the third most species-rich family within Eukalyptorhynchia. However, its diversity and phylogeny have been largely neglected in former studies. We introduce three new genera and twelve new species of Koinocystididae including Simplexcystis asymmetrica gen. n. sp. n., Galapagetula cubensis sp. n., eight species of Reinhardorhynchus gen. n. and two species of Itaipusa. This raises the total number of species within Koinocystididae from 51 to 63. We also report on new distribution records for six known species: I. divae (Cuba, Panama and New Caledonia), I. karlingi (Sardinia and Lanzarote), Reinhardorhynchus riegeri comb. n. (Cuba), R. ruffinjonesi comb. n. (Cuba and Panama), Utelga heinckei (Cuba and Lanzarote), and U. pseudoheinckei (Sardinia). Simplexcystis asymmetrica gen. n. sp. n. is characterised by a male duct running eccentrically through the copulatory bulb, lack of any hard structures in the male system, lack of a bursa, and the fact that the epithelia of the female, the male, and part of the common atrium are covered by a brush border. Galapagetula cubensis sp. n. has a caudal gonopore, a divisa-type copulatory bulb with an unarmed penis papilla, and a female duct without a sphincter. The new species of Itaipusa and Reinhardorhynchus gen. n. differ from their congeners in the detailed structure of the copulatory bulb and especially the hard structures associated with it. In a molecular phylogenetic analysis based on all available 18S and 28S rDNA sequences of koinocystidids, we found support for the monophyly of the family and the genus Utelga Marcus, 1949. The genus Itaipusa is not monophyletic in that I. sinensis forms a clade with Rhinolasius dillonicus, while other species of Itaipusa that have a copulatory bulb armed with hooks form a clade together with Sekerana stolzi. As the type species of Itaipusa (I. divae) is in neither of these clades, we erected a new genus for I. sinensis (Koinogladius gen. n.) and one for species of Itaipusa having a hook-bearing copulatory bulb (Reinhardorhynchus gen. n.), respectively. Whether the remaining species of Itaipusa form a monophylum remains uncertain.

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Labrys khuzestanensis n. sp. (Nematoda, Tylenchidae), a new member of the genus with large labial plate

Zootaxa ◽

10.11646/zootaxa.4671.2.7 ◽

2019 ◽

Vol 4671 (2) ◽

pp. 267-276 ◽

Cited By ~ 2

Author(s):

YOUSEF PANAHANDEH ◽

EBRAHIM POURJAM ◽

JOAQUÍN ABOLAFIA ◽

ALI ROSHAN-BAKHSH ◽

SHIDEH MOJERLOU ◽

...

Keyword(s):

New Species ◽

Phylogenetic Analyses ◽

Molecular Data ◽

Lateral View ◽

Cephalic Region ◽

Palm Tree ◽

Electron Microscopic ◽

Sem Images ◽

Lateral Field ◽

Southwestern Iran

Labrys khuzestanensis n. sp. was recovered from the rhizosphere of a palm tree in southwestern Iran and described using morphological and molecular data. The new species was characterized by combination of the following features: its 590–675 μm long body, finely annulated cuticle in light microscopy (LM), lateral field with two smooth incisures, cephalic region dorso-ventrally flattened with protuberant, elongate and laterally extended labial plate, appearing a V-shaped piece in lateral view, amphidial openings as longitudinal slits confined to labial plate in scanning electron microscopic (SEM) images, stylet 7.5–9.0 μm long, elongated fusiform median bulb with faint valvular apparatus, excretory pore wide with moderately sclerotized duct and elongate filiform tail with finely rounded tip. Compared to four currently known species under the genus, the new species has a remarkably larger and more prominent labial plate as revealed by SEM and trapezoid cephalic region under LM. The molecular phylogenetic analyses of the new species using partial SSU rDNA revealed it has close affinity with three species of Labrys (L. chinensis, L. fuzhouensis and L. filiformis) in Bayesian inference (BI) and maximum likelihood (ML) methods and occupied a placement inside their clade. L. fujianensis, the other species of the genus, occupied a distant placement to the aforementioned clade. This is the second species of Labrys being originally described from Iran, representing the third species of Labrys occurring in the country.

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