Hydrophilic and cationic latex particles for the specific extraction of nucleic acids

1999 ◽  
Vol 10 (4) ◽  
pp. 403-420 ◽  
Author(s):  
Abdelhamid Elaissari ◽  
Lowenna Holt ◽  
Francoise Meunier ◽  
Cécile Voisset ◽  
Christian Pichot ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Latex Particles ◽  
Cationic Latex

A novel approach to nonradioactive hybridization assay of nucleic acids using stained latex particles

1991 ◽  
Vol 198 (2) ◽  
pp. 308-311 ◽  
Author(s):  
T.I. Vener ◽  
M.F. Turchinsky ◽  
V.D. Knorre ◽  
Yu.V. Lukin ◽  
S.N. Shcherbo ◽  
...  
Keyword(s):  
Nucleic Acids ◽  
Hybridization Assay ◽  
Latex Particles ◽  
Novel Approach

scholarly journals Effect of size of latex particles on the mechanical properties of hydrogels reinforced by latex particles

RSC Advances ◽  
2019 ◽  
Vol 9 (26) ◽  
pp. 14701-14707
Author(s):  
Li Liu ◽  
Guangchao Lv ◽  
Xiuyan Ren ◽  
Xinhe Li ◽  
Te Wang ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Cross Linking ◽  
Particle Sizes ◽  
Latex Particles ◽  
Cationic Latex

Herein, cationic latex particles (CL) of different particle sizes were introduced as a cross-linking center to enhance the mechanical properties of the hydrophobically-associated hydrogels (P(AAm-co-HMA)-CL).

Application of cationic latex particles for protein separation

1994 ◽  
Vol 2 (4) ◽  
pp. 419-427 ◽  
Author(s):  
Yoshiteru Sumi ◽  
Toshifumi Shiroya ◽  
Keiji Fujimoto ◽  
Tadashi Wada ◽  
Hiroshi Handa ◽  
...  
Keyword(s):  
Protein Separation ◽  
Latex Particles ◽  
Cationic Latex

Control of Charge Densities for Cationic Latex Particles

2005 ◽  
Vol 38 (9) ◽  
pp. 3653-3662 ◽  
Author(s):  
Dirk-Jan Voorn ◽  
W. Ming ◽  
Alex M. van Herk
Keyword(s):  
Latex Particles ◽  
Charge Densities ◽  
Cationic Latex

Electron Microscopy of Nucleic Acids

1974 ◽  
Vol 32 ◽  
pp. 302-303
Author(s):  
Norman Davidson
Keyword(s):  
Electron Microscopy ◽  
Nucleic Acids ◽  
Basic Protein ◽  
Molecular Biologist ◽  
Topological Properties ◽  
Protein Film ◽  
Polynucleotide Chain

The basic protein film technique for mounting nucleic acids for electron microscopy has proven to be a general and powerful tool for the working molecular biologist in characterizing different nucleic acids. It i s possible to measure molecular lengths of duplex and single-stranded DNAs and RNAs. In particular, it is thus possible to as certain whether or not the nucleic acids extracted from a particular source are or are not homogeneous in length. The topological properties of the polynucleotide chain (linear or circular, relaxed or supercoiled circles, interlocked circles, etc. ) can also be as certained.

A Freeze Shadow Technique for the Preparation of Soft Paint Latexes for Electron Microscopy

1977 ◽  
Vol 35 ◽  
pp. 314-315
Author(s):  
Earl R. Walter ◽  
Glen H. Bryant
Keyword(s):  
Sample Preparation ◽  
High Vacuum ◽  
Vacuum System ◽  
Latex Particles ◽  
New Methods ◽  
Diffusion Pump ◽  
Film Forming ◽  
Routine Basis ◽  
Distribution Studies ◽  
Preparation Techniques

With the development of soft, film forming latexes for use in paints and other coatings applications, it became desirable to develop new methods of sample preparation for latex particle size distribution studies with the electron microscope. Conventional latex sample preparation techniques were inadequate due to the pronounced tendency of these new soft latex particles to distort, flatten and fuse on the substrate when they dried. In order to avoid these complications and obtain electron micrographs of undistorted latex particles of soft resins, a freeze-dry, cold shadowing technique was developed. The method has now been used in our laboratory on a routine basis for several years.The cold shadowing is done in a specially constructed vacuum system, having a conventional mechanical fore pump and oil diffusion pump supplying vacuum. The system incorporates bellows type high vacuum valves to permit a prepump cycle and opening of the shadowing chamber without shutting down the oil diffusion pump. A baffeled sorption trap isolates the shadowing chamber from the pumps.

mRNA localization by Electron microscopic in situ hybridization

1991 ◽  
Vol 49 ◽  
pp. 430-431
Author(s):  
J. A. Pollock ◽  
M. Martone ◽  
T. Deerinck ◽  
M. H. Ellisman
Keyword(s):  
Electron Microscopy ◽  
High Resolution ◽  
Nucleic Acids ◽  
Recombinant Dna ◽  
Light And Electron Microscopy ◽  
Electron Microscopic ◽  
High Voltage Electron Microscopy ◽  
Functional Sites ◽  
Voltage Electron

Localization of specific proteins in cells by both light and electron microscopy has been facilitate by the availability of antibodies that recognize unique features of these proteins. High resolution localization studies conducted over the last 25 years have allowed biologists to study the synthesis, translocation and ultimate functional sites for many important classes of proteins. Recently, recombinant DNA techniques in molecular biology have allowed the production of specific probes for localization of nucleic acids by “in situ” hybridization. The availability of these probes potentially opens a new set of questions to experimental investigation regarding the subcellular distribution of specific DNA's and RNA's. Nucleic acids have a much lower “copy number” per cell than a typical protein, ranging from one copy to perhaps several thousand. Therefore, sensitive, high resolution techniques are required. There are several reasons why Intermediate Voltage Electron Microscopy (IVEM) and High Voltage Electron Microscopy (HVEM) are most useful for localization of nucleic acids in situ.

Nucleic Acid Molecules Prepared by Monolayer Techniques

1972 ◽  
Vol 30 ◽  
pp. 178-179
Author(s):  
Dimitrij Lang
Keyword(s):  
Electron Microscopy ◽  
Standard Deviation ◽  
Nucleic Acid ◽  
Cytochrome C ◽  
Nucleic Acids ◽  
Contour Length ◽  
Sample Standard Deviation ◽  
Molecular Weights ◽  
Length Measurements ◽  
Protein Monolayer

The success of the protein monolayer technique for electron microscopy of individual DNA molecules is based on the prevention of aggregation and orientation of the molecules during drying on specimen grids. DNA adsorbs first to a surface-denatured, insoluble cytochrome c monolayer which is then transferred to grids, without major distortion, by touching. Fig. 1 shows three basic procedures which, modified or not, permit the study of various important properties of nucleic acids, either in concert with other methods or exclusively:1) Molecular weights relative to DNA standards as well as number distributions of molecular weights can be obtained from contour length measurements with a sample standard deviation between 1 and 4%.

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