Relation between Tumor Necrosis Factor-α And Granulocyte Elastase-α1-Proteinase Inhibitor Complexes in the Plasma of Patients with Cystic Fibrosis: Reply

1990 ◽  
Vol 142 (4) ◽  
pp. 985-985
Author(s):  
Jean-Michel Dayer ◽  
Susanne Suter
Keyword(s):  
Cystic Fibrosis ◽  
Tumor Necrosis Factor ◽  
Proteinase Inhibitor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Granulocyte Elastase ◽  
Factor Α ◽  
Necrosis Factor

scholarly journals Tumor necrosis factor-α/interleukin-10 balance in normal and cystic fibrosis children

2001 ◽  
Vol 10 (4) ◽  
pp. 191-197 ◽  
Author(s):  
Galina V. Shmarina ◽  
Alexander L. Pukhalsky ◽  
Svetlana N. Kokarovtseva ◽  
Daria A. Pukhalskaya ◽  
Lidia A. Shabalova ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Interleukin 10 ◽  
Healthy Children ◽  
Linear Association ◽  
Tnf Α ◽  
Factor Α ◽  
Necrosis Factor

Background:The balance between tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10) is important for immune homeostasis maintenance. Exuberant production of TNF-α contributes to overwhelming inflammatory response and tissue damage. But, commonly, increase in TNF-α is counterbalanced by simultaneous synthesis of an anti-inflammatory cytokine IL-10, which suppresses production of many activating and regulatory mediators.Aims:In the present study, the relationships between TNF-α and IL-10 in the plasma of healthy schoolchildren and cystic fibrosis (CF) patients have been investigated.Methods:Blood samples were obtained from 12 CF patients with chronic pulmonary disease and 18 healthy schoolchildren vaccinated with live attenuated rubella vaccine. IL-10 and TNF-α were determined in the plasma samples using commercially available enzyme-linked immunosorbent assay kits.Results:Before vaccination, most healthy children (13 of 18) demonstrated superiority of pro-inflammatory TNF-α over anti-inflammatory IL-10 (TNF-α/IL-10 Â 1). In these subjects, a significant positive linear association between the cytokine values has been found. Vaccine challenge resulted in a marked reduction of TNF-α/IL-10 ratios. In addition, a disappearance of correlation between the cytokine values was observed. Such disturbance was related to exuberant elevation of the IL-10 levels after inoculation. On the contrary, in CF individuals, plasma cytokine values remained in strong linear association independently of TNF-α or IL-10 predominance. No spikes in the plasma levels of IL-10 in CF patients during a 6-month observation period have been revealed.Conclusions:There were no fundamental differences between CF and healthy children in the regulation of TNF-α and IL-10 secretion. Thus, immune quiescence seemed to be associated with the predominance of TNF-α, whereas immune disturbance was characterized by IL-10 superiority. The only abnormality that was found in CF patients consisted of their inability to produce unlimitedly IL-10 in response to antigen stimuli.

scholarly journals Circulating Tumor Necrosis Factor-α Levels and Lipid Abnormalities in Patients with Cystic Fibrosis

1993 ◽  
Vol 34 (2) ◽  
pp. 162-165 ◽  
Author(s):  
Emile Levy ◽  
Covadonga Gurbindo ◽  
Florence Lacaille ◽  
Khazal Paradis ◽  
Louise Thibault ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Factor Α ◽  
Lipid Abnormalities ◽  
Necrosis Factor

Increased elastase release by CF neutrophils is mediated by tumor necrosis factor-α and interleukin-8

2000 ◽  
Vol 278 (1) ◽  
pp. L33-L41 ◽  
Author(s):  
Clifford Taggart ◽  
Raymond J. Coakley ◽  
Peter Greally ◽  
Gerry Canny ◽  
Shane J. O'Neill ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Cystic Fibrosis ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Normal Subjects ◽  
Tnf Α ◽  
Blood Neutrophils ◽  
Factor Α ◽  
Necrosis Factor

Cystic fibrosis (CF) is a lethal, hereditary disorder characterized by a neutrophil-dominated inflammation of the lung. We sought to determine whether neutrophils from individuals with CF release more neutrophil elastase (NE) than neutrophils from normal subjects. Our results showed that peripheral blood neutrophils (PBNs) from normal subjects and individuals with CF contained similar amounts of NE, but after preincubation with CF bronchoalveolar lavage (BAL) fluid, significantly more NE was released by CF PBNs, a release that was amplified further by incubation with opsonized Escherichia coli. To determine which components of CF BAL fluid stimulated this excessive NE release from CF PBNs, we repeated the experiments after neutralization or immunoprecipitation of tumor necrosis factor (TNF)-α and interleukin (IL)-8 in CF BAL fluid. We found that subsequent NE release from CF PBNs was reduced significantly when TNF-α and IL-8 were removed from CF BAL fluid. When TNF-α and IL-8 were used as activating stimuli, CF PBNs released significantly greater amounts of NE compared with PBNs from control subjects and individuals with bronchiectasis. These results indicate that CF PBNs respond abnormally to TNF-α and IL-8 in CF BAL fluid and react to opsonized bacteria by releasing more NE. This may help explain the increased NE burden seen in this condition.

scholarly journals The Tumor Necrosis Factor α (-308 A/G) Polymorphism Is Associated with Cystic Fibrosis in Mexican Patients

PLoS ONE ◽  
2014 ◽  
Vol 9 (3) ◽  
pp. e90945 ◽  
Author(s):  
Celia N. Sanchez-Dominguez ◽  
Miguel A. Reyes-Lopez ◽  
Adriana Bustamante ◽  
Ricardo M. Cerda-Flores ◽  
Maria del C. Villalobos-Torres ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Factor Α ◽  
Necrosis Factor ◽  
Mexican Patients

scholarly journals Modification of transepithelial ion transport in human cultured bronchial epithelial cells by interferon-γ

2000 ◽  
Vol 278 (6) ◽  
pp. L1186-L1194 ◽  
Author(s):  
Luis J. V. Galietta ◽  
Chiara Folli ◽  
Carla Marchetti ◽  
Luca Romano ◽  
Daniela Carpani ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Tumor Necrosis Factor ◽  
Epithelial Cells ◽  
Ion Transport ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Interferon Γ ◽  
Bronchial Epithelial ◽  
Factor Α ◽  
Necrosis Factor

Human bronchial epithelial cells were treated in vitro with interferon-γ or tumor necrosis factor-α to assess their effect on transepithelial ion transport. Short-circuit current measurements revealed that Na+absorption was markedly inhibited by interferon-γ (10–1,000 U/ml). The cystic fibrosis transmembrane conductance regulator was also downregulated by interferon-γ as evident at the protein level and by the decrease in the cAMP-dependent current. On the other hand, interferon-γ caused an increase of the current elicited by apical UTP application, which is due to the activity of Ca2+-dependent Cl−channels. Tumor necrosis factor-α caused few changes in ion transport. Transepithelial fluid transport was measured in normal and cystic fibrosis cells. At rest, both types of cells showed an amiloride-sensitive fluid absorption that was inhibited by interferon-γ but not by tumor necrosis factor-α. Our results show that interferon-γ alters the transepithelial ion transport of cultured bronchial cells. This effect may change the ion composition and/or volume of periciliary fluid.

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