Detection of Cryptococcal Antigen in Bronchoalveolar Lavage Fluid: A Prospective Study of Diagnostic Utility

1992 ◽  
Vol 145 (5) ◽  
pp. 1226-1229 ◽  
Author(s):  
Robert P. Baughman ◽  
Judith C. Rhodes ◽  
Michael N. Dohn ◽  
Harold Henderson ◽  
Peter T. Frame
Keyword(s):  
Bronchoalveolar Lavage ◽  
Prospective Study ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Diagnostic Utility ◽  
Cryptococcal Antigen ◽  
A Prospective Study

A rise of MMP-2 and MMP-9 in bronchoalveolar lavage fluid is associated with acute lung injury after cardiopulmonary bypass in a swine model

Perfusion ◽  
2003 ◽  
Vol 18 (2) ◽  
pp. 107-113 ◽  
Author(s):  
Wolfgang Eichler ◽  
J F Matthias Bechtel ◽  
Jan Schumacher ◽  
Johanna A Wermelt ◽  
Karl-Friedrich Klotz ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Cardiopulmonary Bypass ◽  
Lung Injury ◽  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Swine Model ◽  
Tissue Samples ◽  
Pulmonary Capillary ◽  
A Prospective Study

Postoperative acute lung injury (ALI) contributes to the morbidity and mortality following cardiopulmonary bypass (CPB). To determine whether the presence of matrix metalloproteinases (MMPs) is associated with ALI after CPB, MMP-2 and MMP-9 activities in bronchoalveolar lavage fluid (BALF) were compared with parameters indicating impaired gas exchange. In a prospective study, 17 minipigs were subjected to CPB for 60 min. Before and at five and 180 min after CPB, MMP-2 and MMP-9 were assayed in BALF and the arterial-alveolar gradient of oxygen tension (AaDO2), the pulmonary capillary wedge pressure (PCWP) and the water content of lung tissue samples (Wt) were evaluated and compared with baseline values. MMP-2 and MMP-9 increased significantly 5 minutes (2.1- and 6.2-fold, respectively) and 180 minutes (3.4- and 14.3-fold, respectively) post-CPB. AaDO2 and Wt, but not PCWP, increased significantly 180 minutes after CPB and only AaDO2, but not PCWP or Wt, was significantly correlated with MMP-2 (r/0.66, p/0.006) and MMP-9 (r/0.62, p/0.01). In conclusion, high levels of MMP-2 and MMP-9 in the pulmonary compartment are associated with ALI after CPB.

scholarly journals Diagnostic Value of Bronchoalveolar Lavage Fluid Cryptococcal Antigen-Lateral Flow Immunochromatographic Assay for Pulmonary Cryptococcosis in non-HIV Patients

2020 ◽  
Author(s):  
Hui-Qing Zeng ◽  
Xue-Ying Cai ◽  
Xiao-Bin Zhang ◽  
Dong-Yong Yang ◽  
Li Lin ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Diagnostic Value ◽  
Lateral Flow ◽  
Immunochromatographic Assay ◽  
Pulmonary Cryptococcosis ◽  
Cryptococcal Antigen ◽  
Positive Results ◽  
Lateral Flow Immunochromatographic Assay

Abstract Background: The present study aimed to investigate the diagnostic value of cryptococcal antigen-lateral flow immunochromatographic assay (CrAg-LFA) in bronchoalveolar lavage fluid (BALF) of patients with pulmonary cryptococcosis (PC). Methods: The subjects collected from March 2015 to October 2018 in 4 hospitals were divided into the pulmonary cryptococcosis (PC) group (n=72) and the non-pulmonary cryptococcosis (non-PC) group (n=236). The patients in the PC group were diagnosed by lung biopsy. Results: The sensitivity, specificity, positive and negative predicted values of CrAg-LFA in the serum were 75.0%, 99.6%, 98.2%, and 92.9%, respectively, while those in the BALF were 93.1%, 100.0%, 100.0%, and 97.9%, respectively. Hence, the sensitivity of the CrAg-LFA in BALF was significantly higher than that in the serum of the patients in the PC group (P<0.05). Conclusion: The CrAg-LFA is a rapid, simple and safe experimental method. CrAg-LFA in BALF samples can offer higher diagnostic value for PC than that noted in the serum samples of PC subjects. Furthermore, the BALF positive results were equivalent to the microbiological culture positive results in terms of diagnostic value.

scholarly journals Comparison of Sample Preparation Methods for Detection of Legionella pneumophila in Culture-Positive Bronchoalveolar Lavage Fluids by PCR

1998 ◽  
Vol 36 (7) ◽  
pp. 2120-2122 ◽  
Author(s):  
B. Jaulhac ◽  
M. Reyrolle ◽  
Y. K. Sodahlon ◽  
S. Jarraud ◽  
M. Kubina ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Legionella Pneumophila ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Proteinase K ◽  
Inhibition Rate ◽  
Preparation Methods ◽  
A Prospective Study ◽  
Culture Positive ◽  
Culture Negative

A prospective study was conducted on 25 Legionella pneumophila culture-positive and 98 culture-negative bronchoalveolar lavage fluid samples to compare two DNA preparation methods: a rapid modified Chelex-based protocol and a proteinase K method. PCR was found to be more sensitive with the Chelex-based method (P = 0.03). No difference was found concerning the inhibition rate.

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