scholarly journals Comparison of Sample Preparation Methods for Detection of Legionella pneumophila in Culture-Positive Bronchoalveolar Lavage Fluids by PCR

1998 ◽  
Vol 36 (7) ◽  
pp. 2120-2122 ◽  
Author(s):  
B. Jaulhac ◽  
M. Reyrolle ◽  
Y. K. Sodahlon ◽  
S. Jarraud ◽  
M. Kubina ◽  
...  
Keyword(s):  
Bronchoalveolar Lavage ◽  
Legionella Pneumophila ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Proteinase K ◽  
Inhibition Rate ◽  
Preparation Methods ◽  
A Prospective Study ◽  
Culture Positive ◽  
Culture Negative

A prospective study was conducted on 25 Legionella pneumophila culture-positive and 98 culture-negative bronchoalveolar lavage fluid samples to compare two DNA preparation methods: a rapid modified Chelex-based protocol and a proteinase K method. PCR was found to be more sensitive with the Chelex-based method (P = 0.03). No difference was found concerning the inhibition rate.

A rise of MMP-2 and MMP-9 in bronchoalveolar lavage fluid is associated with acute lung injury after cardiopulmonary bypass in a swine model

Perfusion ◽  
2003 ◽  
Vol 18 (2) ◽  
pp. 107-113 ◽  
Author(s):  
Wolfgang Eichler ◽  
J F Matthias Bechtel ◽  
Jan Schumacher ◽  
Johanna A Wermelt ◽  
Karl-Friedrich Klotz ◽  
...  
Keyword(s):  
Acute Lung Injury ◽  
Cardiopulmonary Bypass ◽  
Lung Injury ◽  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Swine Model ◽  
Tissue Samples ◽  
Pulmonary Capillary ◽  
A Prospective Study

Postoperative acute lung injury (ALI) contributes to the morbidity and mortality following cardiopulmonary bypass (CPB). To determine whether the presence of matrix metalloproteinases (MMPs) is associated with ALI after CPB, MMP-2 and MMP-9 activities in bronchoalveolar lavage fluid (BALF) were compared with parameters indicating impaired gas exchange. In a prospective study, 17 minipigs were subjected to CPB for 60 min. Before and at five and 180 min after CPB, MMP-2 and MMP-9 were assayed in BALF and the arterial-alveolar gradient of oxygen tension (AaDO2), the pulmonary capillary wedge pressure (PCWP) and the water content of lung tissue samples (Wt) were evaluated and compared with baseline values. MMP-2 and MMP-9 increased significantly 5 minutes (2.1- and 6.2-fold, respectively) and 180 minutes (3.4- and 14.3-fold, respectively) post-CPB. AaDO2 and Wt, but not PCWP, increased significantly 180 minutes after CPB and only AaDO2, but not PCWP or Wt, was significantly correlated with MMP-2 (r/0.66, p/0.006) and MMP-9 (r/0.62, p/0.01). In conclusion, high levels of MMP-2 and MMP-9 in the pulmonary compartment are associated with ALI after CPB.

Detection of Cryptococcal Antigen in Bronchoalveolar Lavage Fluid: A Prospective Study of Diagnostic Utility

1992 ◽  
Vol 145 (5) ◽  
pp. 1226-1229 ◽  
Author(s):  
Robert P. Baughman ◽  
Judith C. Rhodes ◽  
Michael N. Dohn ◽  
Harold Henderson ◽  
Peter T. Frame
Keyword(s):  
Bronchoalveolar Lavage ◽  
Prospective Study ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Diagnostic Utility ◽  
Cryptococcal Antigen ◽  
A Prospective Study

scholarly journals Correlation between Sputum and Bronchoalveolar Lavage Fluid Cultures

2014 ◽  
Vol 53 (3) ◽  
pp. 994-996 ◽  
Author(s):  
Grégory Dubourg ◽  
Cédric Abat ◽  
Jean-Marc Rolain ◽  
Didier Raoult
Keyword(s):  
Bronchoalveolar Lavage ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Correlation Study ◽  
Microbiologic Diagnosis ◽  
Cultured Bacteria ◽  
Culture Positive

A correlation study of the cultured bacteria from paired sputum and bronchoalveolar lavage fluid samples was performed. The rates of concordant culture-positive paired specimens that were isolated within 1 or 7 days were 93.7% and 96.5%, respectively, suggesting that the culture of readily collectable sputum specimens may result in useful microbiologic diagnosis.

scholarly journals Detection of Legionella pneumophila Serogroup 1 Antigen in Bronchoalveolar Lavage Fluid by an Immunochromatographic Assay

2003 ◽  
Vol 41 (5) ◽  
pp. 2265-2265 ◽  
Author(s):  
P. C. Wever ◽  
D. W. Notermans ◽  
I. I. Tulevski ◽  
J. K. M. Eeftinck Schattenkerk ◽  
M. D. d. Jong
Keyword(s):  
Bronchoalveolar Lavage ◽  
Legionella Pneumophila ◽  
Bronchoalveolar Lavage Fluid ◽  
Lavage Fluid ◽  
Immunochromatographic Assay
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