A taxonomic reassessment of Viburnum (Adoxaceae) in the Azores

The taxonomic status of the Azorean endemic Viburnum tinus subsp. subcordatum is reassessed, using morphological characters and new molecular data from the ITS region and the trnK intron. A survey of morphological variation supports the recognition of V. tinus subsp. subcordatum as distinct from V. tinus subsp. tinus and the Canary endemic V. rugosum (formerly known as V. tinus subsp. rigidum) based on leaf shape, the shape of the leaf base and apex, the sub-entire and revolute leaf margins, blistered upper leaf surfaces, trichome density and type, and fruit size. Molecular data also confirm this distinctiveness within section Tinus. Taken together, our morphological and genetic data presented in this paper support the recognition of the Azorean taxon at the species level under the name of Viburnum treleasei. A description of the species is provided and nomenclatural issues relating to the two Macaronesian Viburnum taxa are discussed.

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Amanita subjunquillea and its ectomycorrhizal association, reported as new for Pakistan

Mycotaxon ◽

10.5248/134.413 ◽

2019 ◽

Vol 134 (3) ◽

pp. 413-423

Author(s):

Muhammad Ishaq ◽

Arooj Naseer ◽

Munazza Kiran ◽

Muhammad Fiaz ◽

Abdul Nasir Khalid

Keyword(s):

Its Region ◽

Morphological Characters ◽

Molecular Data ◽

Sequence Analyses ◽

Dark Orange ◽

Ectomycorrhizal Roots ◽

Morphological And Molecular Data ◽

Quercus Floribunda ◽

First Time ◽

Ectomycorrhizal Association

Amanita subjunquillea and its ectomycorrhizal association are reported for the first time from moist temperate Himalayan forests of Pakistan. The sample was studied based on morphological characters and nucleotide sequence analyses of the ITS region generated from basidiomata and ectomycorrhizal roots of Quercus floribunda. Our collection differs from the type in its dark orange pileus disc and pale yellow margins. Remaining morphological and molecular data are consistent with previously reported specimens. This represents the first report of A. subjunquillea from Pakistan.

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Variability, host range, delimitation and neotypification of Amanita simulans (Amanita section Vaginatae): collections associated with Helianthemum grasslands, and epitypification of A. lividopallescens

Phytotaxa ◽

10.11646/phytotaxa.280.1.1 ◽

2016 ◽

Vol 280 (1) ◽

pp. 1 ◽

Cited By ~ 1

Author(s):

ALFREDO VIZZINI ◽

MIRCA ZOTTI ◽

MIDO TRAVERSO ◽

ENRICO ERCOLE ◽

PIERRE-ARTHUR MOREAU ◽

...

Keyword(s):

Phylogenetic Analysis ◽

Morphological Variability ◽

Taxonomic Status ◽

Morphological Characters ◽

Molecular Data ◽

Phylogenetic Position ◽

Extensive Discussion ◽

Closely Related Species ◽

Colour Form ◽

Morphological And Molecular Data

Several collections of Amanita species from section Vaginatae have been reported to be strictly associated with Helianthemum plants growing in grasslands, a still largely under-explored ectomycorrhizal habitat. The main aim of this study was to investigate the taxonomic status and phylogenetic position of strictly Helianthemum-associated Amanita sect. Vaginatae taxa, informally named Amanita “helianthemicola”. Collections from Italy, England and France were included in this study. The morphologically closely related species complex A. lividopallescens was also examined. Analyses were carried out based on both morphological and molecular data (phylogenetic analysis of the nrITS sequences). All investigated Amanita collections, which are strictly associated with Helianthemum nummularium, turned out to be conspecific with A. simulans. Amanita simulans was recently described from Sardinia (Italy) from Populus nigra habitats based on morphological characters only. As the holotype of A. simulans was lost, a neotype is designated here based on a voucher from the original collecting area. Amanita simulans is re-described, and an extensive discussion on the morphological variability, host species range, distribution and related taxa is provided. Amanita lividopallescens was confirmed as a good species, and re-delimited based on our phylogenetic analysis; moreover, it was epitypified with a recent and well-documented collection from Corse (France). Amanita stenospora is a synonym of A. lividopallescens, being a colour form of the latter without taxonomic value.

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Cytogenetic and sequence comparison of adultPhyllodistomum(Digenea: Gorgoderidae) from the three-spined stickleback with larvae from two bivalves

Parasitology ◽

10.1017/s0031182004006109 ◽

2004 ◽

Vol 129 (6) ◽

pp. 771-778 ◽

Cited By ~ 14

Author(s):

R. PETKEVIČIŪTĖ ◽

V. STUNŽĖNAS ◽

G. STANEVIČIŪTĖ

Keyword(s):

Developmental Stages ◽

Its Region ◽

Morphological Characters ◽

Molecular Data ◽

Chromosome Morphology ◽

Published Data ◽

Larval Stages ◽

Rdna Sequences ◽

Rdna Sequencing ◽

High Level

Due to the low informative value of available morphological characters, cytogenetic and molecular methods, based on rDNA sequencing, were used to characterize adult and larval stages ofPhyllodistomumspp. Species studied have 18 chromosomes with comparable absolute and relative lengths. Conventional Giemsa staining and karyometric analysis revealed clear differences in chromosome morphology of larvalPhyllodistomumspp. infecting two bivalve host species,Sphaerium corneumandPisidium amnicum. However, karyotypes of adultP. foliumfrom three-spined sticklebacks and larval stages fromS. corneumappear almost identical both with respect to the relative lengths and centromeric indices of the corresponding chromosome pairs. The entire internal transcribed spacer (ITS) region (ITS-1, 5.8S and ITS-2) and the D1-D3 region of 28S gene were sequenced and compared. Again, sufficient differences were observed between larvalPhyllodistomumspp., while adultP. foliumand larvae fromS. corneumshowed a high level of similarity. So, both cytogenetic and molecular data support the suggestion that they represent developmental stages of the same species. The results were compared with published data obtained by cytogenetic and molecular studies on the otherPhyllodistomumspecies. Differences revealed in karyotype and rDNA sequences leads to the conclusion that the cercariaeum ofP. foliumsensu Sinitsin, 1905 could not be regarded as the larva of adultP. foliumfrom three-spined stickleback.

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Pseudosperma albobrunneum sp. nov. from coniferous forests of Pakistan

Mycotaxon ◽

10.5248/136.361 ◽

2021 ◽

Vol 136 (2) ◽

pp. 361-372

Author(s):

Sana Jabeen ◽

Zainab ◽

Hira Bashir ◽

Abdul Nasir Khalid

Keyword(s):

Phylogenetic Analysis ◽

New Species ◽

Its Region ◽

Morphological Characters ◽

Molecular Data ◽

Nuclear Ribosomal Dna ◽

Coniferous Forests ◽

Molecular Phylogenetic ◽

Khyber Pakhtunkhwa Province ◽

A New Species

A new species, Pseudosperma albobrunneum, is described and illustrated. The specimens are from different areas of Khyber Pakhtunkhwa province, Pakistan. The identification is based on morphological characters, in combination with molecular phylogenetic analysis of sequences of the ITS region of nuclear ribosomal DNA. The species is found distinct morphologically from all closely related taxa, and molecular data confirm its novelty.

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Taxonomic status of Pelargonium reniforme Curt.

Bothalia ◽

10.4102/abc.v44i1.173 ◽

2014 ◽

Vol 44 (1) ◽

Author(s):

Janine E. Victor ◽

Mmamphe Aphane

Keyword(s):

Conservation Status ◽

Leaf Shape ◽

Flower Colour ◽

Taxonomic Status ◽

Morphological Characters ◽

Herbarium Specimens ◽

Current Investigation ◽

Petiole Length ◽

Pelargonium Sidoides ◽

Variable Species

Background: Pelargonium reniforme Curt. is a morphologically variable species that many authors have attempted to split or combine. Confusion relating to the differences between the two subspecies currently included under P. reniforme has impeded attempts to assess their conservation status. Pelargonium reniforme is closely related to Pelargonium sidoides; the two species are indistinguishable when not flowering and their distributions overlap in some areas.Objectives: With this study, we aimed to clarify the taxonomic status of the two subspecies of P. reniforme, which has relevance in terms of their conservation status.Method: Leaf shape, petiole length, internode length and flower colour were assessed by studying herbarium specimens of the two subspecies of P. reniforme and specimens of P. sidoides. Living specimens of the two subspecies were also examined in their natural habitat.Results: The current investigation showed that the morphological characters used to distinguish the two subspecies of P. reniforme are too variable to separate them. Variation in some morphological characters may be related to environmental conditions.Conclusion: The recognition of the two subspecies of P. reniforme as distinct taxa is no longer justified.

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Heliocybe villosa sp. nov., a new member to the genus Heliocybe (Gloeophyllales)

Phytotaxa ◽

10.11646/phytotaxa.349.2.8 ◽

2018 ◽

Vol 349 (2) ◽

pp. 173 ◽

Cited By ~ 1

Author(s):

MING ZHANG ◽

TAI-HUI LI ◽

BIN SONG

Keyword(s):

Phylogenetic Analysis ◽

Internal Transcribed Spacer ◽

Southern China ◽

Its Region ◽

Morphological Characters ◽

Molecular Data ◽

Hyphal System

Heliocybe villosa sp. nov. from southern China, is described, illustrated and discussed here based on its distinct morphological characters and molecular data. It is characterized by a yellowish brown pileus densely covered with orange brown villous hairs or flocci, white and close lamellae with serrated edge, broadly cylindric to subreniform basidiospores 7–9 × 3–3.5 μm, and monomitic hyphal system without clamp connections. Phylogenetic analysis based on the internal transcribed spacer (ITS) region further confirmed its placement in Heliocybe, and the second recorded species for this genus.

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A new species of Noccaea (Brassicaceae) from central Anatolia

Phytotaxa ◽

10.11646/phytotaxa.432.1.8 ◽

2020 ◽

Vol 432 (1) ◽

pp. 95-103

Author(s):

KURTULUŞ ÖZGİŞİ

Keyword(s):

New Species ◽

Its Region ◽

Morphological Characters ◽

Molecular Data ◽

Iucn Red List ◽

Red List ◽

Central Anatolia ◽

Distribution Map ◽

Closely Related Species ◽

A New Species

Noccaea atila-ocakii is described as a new species from Eskisehir, Turkey. Morphologically, it is similar to N. antitaurica and N. lilacina, but it is easily distinguished by several morphological characters, including the size of its basal leaves, shape of its cauline leaves and fruit, length of its style, number of ovules, etc. Phylogenetic relationships, based on the nuclear-encoded ribosomal internal transcribed spacer (ITS) region, within the genus are shown. Molecular data reveal that new species clearly differs from N. antitaurica and N. lilacina. Paraphyletic taxa in the phylogenetic tree also indicate that the generic circumscription of Noccaea by Al-Shehbaz is more convenient compared with previous delimitations of this genus. Diagnostic characters, a detailed description, and photographs of the new species are given; the IUCN Red List category and observations on the ecology of the populations are noted; and the distribution map of the new species and its closely related species in Turkey are presented.

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Taxonomy of the Oulimnius tuberculatus species group (Coleoptera: Elmidae) based on molecular and morphological data

Zootaxa ◽

10.11646/zootaxa.2670.1.4 ◽

2010 ◽

Vol 2670 (1) ◽

pp. 59 ◽

Cited By ~ 1

Author(s):

FEDOR ČIAMPOR JR. ◽

JÁN KODADA

Keyword(s):

Taxonomic Status ◽

Distinct Species ◽

Morphological Characters ◽

Molecular Data ◽

Species Group ◽

Morphological Data ◽

Cytochrome Oxidase Subunit ◽

Mitochondrial Cytochrome Oxidase Subunit ◽

16S Rna ◽

Mitochondrial Cytochrome Oxidase

Two mitochondrial (cytochrome oxidase subunit I, 16S RNA) and one nuclear (18S RNA) gene fragments were used to analyse relationships within the Oulimnius tuberculatus species group. The results of molecular data analysis are compared with morphological characters, and the distributional patterns and taxonomic status of recognized taxa are addressed. Based on molecular data, Oulimnius perezi is re-validated as a distinct species, and the discovery of a possible new species from northwest Italy is reported. The possible origins and times of speciation within the species group are discussed.

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Taxonomic status of three controversial species of Cephalanthera (Orchidaceae) from Korea based on morphological and molecular data

Phytotaxa ◽

10.11646/phytotaxa.415.2.2 ◽

2019 ◽

Vol 415 (2) ◽

pp. 105-112

Author(s):

HYE WOO SHIN ◽

CHANG SHOOK LEE ◽

SUN A CHOI ◽

SANG MI EUM ◽

NAM SOOK LEE

Keyword(s):

Chloroplast Dna ◽

Nuclear Dna ◽

Taxonomic Status ◽

Distinct Species ◽

Morphological Characters ◽

Molecular Data ◽

The Other ◽

Morphological And Molecular Data ◽

Independent Species

The taxonomic status of three of the seven Cephalanthera species in Korea is controversial: C. erecta var. oblanceolata, C. subaphylla, and C. shizuoi. To clarify their taxonomic status, we used molecular data of ITS of nuclear DNA, and three regions of chloroplast DNA (matK, rpl16 and trnL-F), in addition to morphological characters. We analyzed 82 accessions collected from 26 sites including the other four taxa: C. erecta, C. falcata, C. longibractea, and C. longifolia. The analysis of ITS, matK, rpl16 and trnL-F data showed that Cephalanthera erecta var. oblanceolata should be treated as a synonym of C. erecta. Molecular data supported that C. subaphylla is an independent species rather than a variety or forma of C. erecta. Cephalanthera shizuoi was currently treated as a synonym of C. erecta or C. longifolia based on morphology and the molecular data supported that C. shizuoi should not be recognized as distinct species; but C. shizuoi should be a synonym of C. longifolia, not a synonym of C. erecta.

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Silene sunhangii (Caryophyllaceae), a new species from China

PhytoKeys ◽

10.3897/phytokeys.135.36426 ◽

2019 ◽

Vol 135 ◽

pp. 59-69

Author(s):

Nan Lin ◽

Dai-Gui Zhang ◽

Xian-Han Huang ◽

Jian-Wen Zhang ◽

Jing-Yuan Yang ◽

...

Keyword(s):

New Species ◽

Taxonomic Status ◽

Its Region ◽

Distinct Species ◽

Molecular Data ◽

Central China ◽

Morphological Data ◽

Nuclear Its ◽

Morphological And Molecular Data ◽

A New Species

Silene sunhangii, a new species of Caryophyllaceae known from only three populations in Hubei and Hunan provinces of central China, is described. Both morphological and molecular data were used to assess the taxonomic status and relationships of this species. Morphologically, S. sunhangii is most similar to S. platyphylla Franch. from which it differs most readily in having 3-veined elliptical leaves without pubescence, tasseled catacorolla, pale purple to red petals without a linear lobe or narrow tooth and lanceolate, bifid to one third. A phylogenetic analysis based on nuclear ITS region identified the new species as a well-supported, independent lineage. Our new species is nested within a grade that encompasses species representing a polyphyletic Silene sect. Physolychnis (Benth.) Bocquet. Both the genetic and morphological data support the recognition of Silene sunhangii as a distinct species, although there is inconsistency between these two datasets as to the relationships of the new species.

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