­Phylogenetic Analysis of the Genus Pohlia (Bryophyta, Bryaceae) Using Chloroplast and Nuclear Ribosomal DNA

Phytotaxa ◽  
2018 ◽  
Vol 351 (2) ◽  
pp. 141 ◽  
Author(s):  
JING-YUAN NIU ◽  
☆LIN LI Li ◽  
SHUO SHI ◽  
HUI LI ◽  
XIAO-RUI WANG ◽  
...  

The genus Pohlia Hedwig is a large moss genus that has been placed in the Bryaceae family as traditionally determined. However, a molecular phylogenetic analysis has indicated that the Pohlia genus is more closely related to genera that are traditionally classified into the Mniaceae or Mielichhoferiaceae families. In this study, we performed a molecular phylogenetic analysis using evidence from four chloroplast regions (atpB-rbcL, rps4, trnG, and trnL-trnF) and one nuclear ribosomal intragenic spacer region (ITS) using different methods (maximum parsimony, maximum likelihood, and Bayesian inference). The phylogenetic relationships between Pohlia and its related genera are discussed.         Two large clades among our samples were well supported in all of the datasets. The traditionally defined Bryaceae species, including Brachymenium pendulum, Bryum caespiticium, Bryum capillare, Anomobryum gemmigerum, and Rhodobryum ontariense, formed a monophyletic clade with high support. The second clade corresponding to the Mielichhoferiaceae-Mniaceae complex also had high support. All of the Pohlia, Mielichhoferia and Mniaceae samples were included in this latter clade.         The clearest result from the phylogenetic analysis is that the Pohlia genus is non-monophyletic. The analysis is identical when using three methods and concludes that Pohlia is closer to the genera in Mielichhoferiaceae and Mniaceae. A formal taxonomic treatment of this clade was not performed because we lacked sufficient specimens of the species, although this work will be included in a future study.

PhytoKeys ◽  
2021 ◽  
Vol 183 ◽  
pp. 21-35
Author(s):  
Neng Wei ◽  
Fredrick Munyao Mutie ◽  
Geoffrey Mwachala ◽  
Olwen M. Grace ◽  
Guang-Wan Hu ◽  
...  

Euphorbia mbuinzauensis, a succulent new species of the Synadenium group in Euphorbiaceae from Makueni County, Kenya, is described and illustrated. Morphologically, it is most similar to E. pseudomollis, but differs mainly by its shrubby habit (up to 4 m), abaxial leaves surfaces with densely stellate hairs, 2–4-forked cymes, smaller bracts (ca. 2.5 × 3.0 mm), smaller cyathia (6 mm wide), crimson glands without narrow smooth margin, smaller fruits (ca. 8 × 7 mm) and ovoid seeds (ca. 1.8 × 2.2 mm). Furthermore, we performed a molecular phylogenetic analysis of the Synadenium group in Euphorbia sect. Monadenium, based on complete nuclear ribosomal DNA (nrDNA) datasets. This phylogenetic inference also supports it to be a distinct species. The new species is assessed as Endangered using the IUCN criteria.


Zootaxa ◽  
2020 ◽  
Vol 4851 (2) ◽  
pp. 271-288
Author(s):  
I. BEVERIDGE ◽  
A. JABBAR ◽  
A. KOEHLER ◽  
T. SUKEE

A phylogenetic analysis of the genera of the strongyloid sub-family Cloacininae from macropodoid marsupials in Australasia was undertaken based on morphological characteristics and analysis of concatenated sequences (ITS+) of the first (ITS-1) and second (ITS-2) internal transcribed spacers of nuclear ribosomal DNA. Neither approach provided a robust phylogeny, but similarities between the two methods in terms of generic groupings suggested that substantial revision is needed of the current phenetic classification, with some of the key morphological characteristics currently used to define genera and tribes proving to be homoplasious. 


Phytotaxa ◽  
2018 ◽  
Vol 348 (4) ◽  
pp. 279 ◽  
Author(s):  
ZIA ULLAH ◽  
SANA JABEEN ◽  
HABIB AHMAD ◽  
ABDUL NASIR KHALID

Inocybe pakistanensis is described and illustrated as a new species based on morphological characters and molecular phylogenetic analysis of nuclear ribosomal DNA including the internal transcribed spacer (ITS) regions along with larger subunit (LSU). The distinctive basidiomata have a highly rimose and fibrillose golden brown pileus with a reddish brown, prominent umbo; ellipsoid to amygdaliform, slightly phasoeliform smooth basidiospores; and clamped septa in all the tissues. Molecular phylogenetic analysis supports the placement of I. pakistanensis in section Rimosae s. str.


ZooKeys ◽  
2021 ◽  
Vol 1021 ◽  
pp. 19-35
Author(s):  
Liang-Jing Yang ◽  
Zhi-Min Chang ◽  
Lin Yang ◽  
Xiang-Sheng Chen

A new species Euxaldar daweishanensis Yang, Chang & Chen, sp. nov. is described and illustrated from southwestern China. The female genitalia of the genus Euxaldar is described and presented for the first time. A checklist and key to the known species of the genus are provided. A revised molecular phylogenetic analysis of the family Issidae based on combined partial sequences of 18S, 28S, COI, and Cytb is provided using both Maximum likelihood and Bayesian inference analyses.


Phytotaxa ◽  
2015 ◽  
Vol 213 (3) ◽  
pp. 159 ◽  
Author(s):  
Yousheng Chen ◽  
Qian Yuan

Twenty-six new species of Saussurea (Asteraceae, Cardueae) from the Qinghai-Tibetan Plateau and adjacent regions are described and illustrated. Our molecular phylogenetic analysis based on the nuclear ribosomal DNA internal transcribed spacer (ITS) and two chloroplast DNA fragments (trnL-F and psbA-trnH) has shown that most of the new species are well recognized in the molecular tree, with their taxonomic affinities also largely revealed.


2019 ◽  
Author(s):  
Kirill V. Mikhailov ◽  
Viatcheslav N. Ivanenko

AbstractReanalysis of the dataset used by Khodami et al. (2017) reveals low support values for the key nodes of the copepod (Crustacea) phylogeny and fails to reproduce the results obtained in the study. Maximum likelihood (ML) and Bayesian analyses with the dataset produce phylogenies that are inconsistent with the branching of copepod groups proposed by Khodami et al. (2017). The proposed phylogeny is refuted by the approximately unbiased (AU) statistical test, which undermines several conclusions drawn from the original study.


Author(s):  
Alevcan Kaplan ◽  
Alaattin Selçuk Ertekin ◽  
Esra Gündüzler

Leguminosae or Fabaceae is the third-largest flowering plant family and is important in terms of both food production and soil fertility. Wild Vicia species and the genetic diversity of the Southeastern Anatolia Region provide an invaluable resource for the improvement of cultivated temperate feed and legume crops. The rapid progress of technology in recent years has nowmade it possible to use modern techniques in phylogenetic studies and to examine plants in a greater detail using biochemical, cytological and molecular methods to supplement purely systematic studies. The use of molecular phylogenetic analysis is the most attractive alternative strategy for a more accurate identification of the species of the Vicia genus. In the current study, some Vicia L. taxa growing naturally in the Southeastern Anatolia Region were investigated using molecular phylogenetic analysis. Internal transcribed spacers (ITS) of nuclear ribosomal DNA were sequenced in order to study the phylogenetic relationships of Vicia L. taxa. Lathyrus inconspicuous L. and Lathyrus cassius Boiss. were used as an outgroup. The ITS area was determined to be approximately 479- 672 bp. The ITS sequences were submitted to the NCBI database and accession numbers obtained. The resulting tree clearly groups and separates the sect. Narbonensis, Ervilia, Peregrinae, Lathyroides, Vicia and Cracca species but was less able to distinguish species from sect. Hypechusa and Lentopsis. The data acquired were observed to be reliable in terms of solving the taxonomical problems of the Vicia L. taxa. The morphological distinctions are greatly supported by DNA sequence studies. The species-specific markers developed in this study are useful for early detection of targeted Vicia taxa and can act as a guide to the basic data required for the evolution of systematic breeding and conservation strategies, as well as for germplasm resources.


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