Immunogenic Properties of MVs Containing Structural Hantaviral Proteins: An Original Study

Hemorrhagic fever with renal syndrome (HFRS) is an emerging infectious disease that remains a global public health threat. The highest incidence rate is among zoonotic disease cases in Russia. Most cases of HFRS are reported in the Volga region of Russia, which commonly identifies the Puumala virus (PUUV) as a pathogen. HFRS management is especially challenging due to the lack of specific treatments and vaccines. This study aims to develop new approaches for HFRS prevention. Our goal is to test the efficacy of microvesicles (MVs) as PUUV nucleocapsid (N) and glycoproteins (Gn/Gc) delivery vehicles. Our findings show that MVs could deliver the PUUV N and Gn/Gc proteins in vitro. We have also demonstrated that MVs loaded with PUUV proteins could elicit a specific humoral and cellular immune response in vivo. These data suggest that an MV-based vaccine could control HFRS.

The Translation and Validation of The Smartphone Use Questionnaire (SUQ) Into The Malay Language

Interruptions caused by frequent smartphone use steals attention away from daily activities, bringing serious implications onto an individual’s health, safety and education. Smartphone Use Questionnaire (SUQ) is a 20-item questionnaire developed to assess the pattern of smartphone use and its effect on attention. This study was done to translate and validate the Malay-language version of the SUQ and to measure the psychometric properties of the Malay-version SUQ to justify its use in Malaysia. A forward and back-translation was done by four individuals, who were three physicians and one linguist. Content and face validity was done involving three experts who were a linguist, psychiatrist and epidemiologist. Psychometric testing was conducted on a sample of 195 individuals proficient in the Malay language. A construct validity test was performed using factor analysis and the internal reliability was tested by calculating for the Cronbach’s Alpha. The age range of the sample was 13-59 years, most of which were female and of the Malay race. Using principal component analysis with direct oblimin rotation, the factor analysis extracted two components similar to the original study: General Use and Absent-Minded Use. However, question number 20 was grouped into General Use component, whereas in the original study it was under the Absent- Minded Use component. The Cronbach’s Alpha for the obtained components was 0.884 and 0.927, respectively. This study found that the Malay-version SUQ was a valid and reliable instrument for use in Malaysia in assessing inattention associated with smartphone use.

The Pragmatic American Revisited: A Direct Replication of Pickett and Baker (2014)

In 2014, Pickett and Baker cast doubt on the scholarly consensus that Americans are pragmatic about criminal justice. Previous research suggested this pragmaticism was evidenced by either null or positive relationships between seemingly opposite items (i.e., between dispositional and situational crime attributions and between punitiveness and rehabilitative policy support). Pickett and Baker (2014) argued that because these studies worded survey items in the same positive direction, respondents’ susceptibility to acquiescence bias led to artificially inflated positive correlations. Using a simple split-ballot experiment, they manipulated the direction of survey items and demonstrated bidirectional survey items resulted in negative relationships between attributions and between support for punitive and rehabilitative policies. We replicated Pickett and Baker’s (2014) methodology with a nationally representative sample of American respondents supplemented by a diverse student sample. Our results were generally consistent, and, in many cases, effect sizes were stronger than those observed in the original study. Americans appear much less pragmatic when survey items are bidirectional. Yet, we suggest the use of bidirectional over unidirectional survey items trades one set of problems for another. Instead, to reduce acquiescence bias and improve overall data quality, we encourage researchers to adopt item-specific questioning.

Deliberation in health-related headlines

Just how big of a difference will deliberated thinking make in the digital age when judging whether theheadline of a digital article is true or fake? Misinformation plagued the Chinese internet space, and fakenews, especially related to health tips, often went viral on the internet with rapid speed. A previous study 1was previously conducted on political articles measuring the influence of partisanship on thinkingdeliberately. In this paper, we conducted a study on how deliberation influenced the accuracy of Chinesenetizens distinguishing real and fake news headlines, using a similar experiment procedure from theabove mentioned study. We found that deliberation reduces the possibility of these readers beingmisguided by fake health-related headlines. A similar trend of accuracy was observed when participantsthought deliberately compared to the original study, despite using different topics on a differentpopulation of participants.

Analysing the Protein-DNA Binding Sites in Arabidopsis thaliana from ChIP-seq Experiments

Computational genomics aim at supporting the discovery of how the functionality of the genome of the organism under study is affected both by its own sequence and structure, and by the network of interaction between this genome and different biological or physical factors. In this work, we focus on the analysis of ChIP-seq data, for which many methods have been proposed in the recent years. However, to the best of our knowledge, those methods lack an appropriate mathematical formalism. We have developed a method based on multivariate models for the analysis of the set of peaks obtained from a ChIP-seq experiment. This method can be used to characterize an individual experiment and to compare different experiments regardless of where and when they were conducted. The method is based on a multivariate hypergeometric distribution, which fits the complexity of the biological data and is better suited to deal with the uncertainty generated in this type of experiments than the dichotomous models used by the state of the art methods. We have validated this method with Arabidopsis thaliana datasets obtained from the Remap2020 database, obtaining results in accordance with the original study of these samples. Our work shows a novel way for analyzing ChIP-seq data.

Immersion and Socio-Emotional Experiences During a Movie – Polish Adaptation of the Movie Consumption Questionnaires

The aim of the article is to present a description of the psychometric properties of the Polish adaptation of the Movie Consumption Questionnaires (MCQ) – Immersion and MCQ – Experience. The instrument, created by Fornerino, Helme-Guizon and Gotteland (2008) allows measuring movie consumption in terms of immersion, the intensity of emotions and the intensity of social interactions during the screening. The Polish adaptation of the tool involved two stages, whose participants were movie-goers attending screening events at a cinema ( N=577). The factor structure of the adapted instrument strongly resembles that obtained in the original study (RMSEA<0.08, CFI>0.95, SRNR<0.08) and has demonstrated very good internal consistency (α>0.8, ω>0.8). Criterion validity was also verified through the use of questionnaires that examine the following: affect – PANAS-X (Fajkowska & Marszał-Wiśniewska, 2009), emotionality – HEXACO (Szarota et al., 2007) and mindfulness – Mindful Attention Awareness Scale (Skala Świadomej Obecności; Radoń, 2014). Results of the analyses suggest that the adapted scales may be used as satisfactory tools for measuring movie consumption. By the same token, they may be useful in further psychological exploration of the movie screening phenomenon, with particular regard to immersion, emotional reactions and social interactions of the viewer.

Comparison of the predictive value of two international guidelines for safe discharge of patients with mild traumatic brain injuries and associated intracranial pathology

Purpose To determine and compare the sensitivity, specificity, and proportion of patients eligible for discharge by the Brain Injury Guidelines and the Mild TBI Risk Score in patients with mild traumatic brain injury and concomitant intracranial injury. Methods Retrospective review of the medical records of adult patients with traumatic intracranial injuries and an initial Glasgow Coma Scale score of 14–15, who sought care at Helsingborg Hospital between 2014/01/01 and 2019/12/31. Both guidelines were theoretically applied. The sensitivity, specificity, and percentage of the cohort that theoretically could have been discharged by either guideline were calculated. The outcome was defined as death, in-hospital intervention, admission to the intensive care unit, requiring emergency intubation due to intracranial injury, decreased consciousness, or seizure within 30 days of presentation. Results Of the 538 patients included, 8 (1.5%) and 10 (1.9%) were eligible for discharge according to the Brain Injury Guidelines and the Mild TBI Risk Score, respectively. Both guidelines had a sensitivity of 100%. The Brain Injury Guidelines had a specificity of 2.3% and the Mild TBI Risk Score had a specificity of 2.9%. Conclusion There was no difference between the two guidelines in sensitivity, specificity, or proportion of the cohort eligible for discharge. Specificity and proportion of cohort eligible for discharge were lower than each guideline’s original study. At present, neither guideline can be recommended for implementation in the current or similar settings.

Effectiveness of a numeracy intelligent tutoring system in kindergarten: A conceptual replication

Intelligent Tutoring Systems are a genre of highly adaptive software providing individualized instruction. The current study was a conceptual replication of a previous randomized control trial that incorporated the intelligent tutoring system Native Numbers, a program designed for early numeracy instruction. As a conceptual replication, we kept the method of instruction, the demographics, the number of kindergarten classrooms (n = 3), and the same numeracy and intrinsic motivation screeners as the original study. We changed the time of year of instruction, changed the control group to a wait-control group, added a maintenance assessment for the first group of participants, and included a mathematical language assessment. Analysis of within- and between-group differences using repeated measures ANOVA indicated gains of numeracy were significant only after using Native Numbers (Partial Eta Square = 0.147). Results of intrinsic motivation and mathematical language were not significant. The effect size of numeracy achievement did not reach that of the original study (Partial Eta Square = 0.622). Here, we compared the two studies, discussed plausible reasons for differences in the magnitude of effect sizes, and provided suggestions for future research.

Replication Study: RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth

As part of the Reproducibility Project: Cancer Biology, we published a Registered Report (Bhargava et al., 2016) that described how we intended to replicate selected experiments from the paper "RAF inhibitors prime wild-type RAF to activate the MAPK pathway and enhance growth" (Hatzivassiliou et al., 2010). Here we report the results. We found two unrelated RAF inhibitors, PLX4720 or GDC-0879, selectively inhibited BRAF(V600E) cell proliferation, while the MEK inhibitor, PD0325901, inhibited BRAF(V600E), wild-type RAF/RAS, and mutant RAS cancer cell proliferation, similar to the original study (Figure 1A; Hatzivassiliou et al., 2010). We found knockdown of CRAF, but not BRAF, in mutant RAS cells attenuated the phospho-MEK induction observed after PLX4720 treatment, similar to the original study (Figure 2B; Hatzivassiliou et al., 2010). The original study reported analogous results with GDC-0879, which was not observed in this replication, although unexpected control results confound the interpretation. We also attempted a replication of an assay with recombinant proteins to test the differential effect of RAF inhibitors on BRAF-CRAF heterodimerization (Figure 4A; Hatzivassiliou et al., 2010). Although we were unable to conduct the experiment as planned, we observed differential binding of BRAF by RAF inhibitors; however, it was between BRAF and beads, independent of CRAF. While these data were unable to address whether, under the conditions of the original study, the same observations could be observed, we discuss key differences between the original study and this replication that are important to consider for further experiments. Finally, where possible, we report meta-analyses for each result.