Two new cryptogonimid genera Beluesca n. gen. and Chelediadema n. gen. (Digenea: Cryptogonimidae) from tropical Indo-West Pacific  Haemulidae (Perciformes)

Zootaxa ◽  
2007 ◽  
Vol 1543 (1) ◽  
pp. 45-60 ◽  
Author(s):  
TERRENCE L. MILLER ◽  
THOMAS H. CRIBB
Keyword(s):  
Dna Sequence ◽  
Host Preference ◽  
Sequence Data ◽  
Large Subunit ◽  
Internal Transcribed Spacers ◽  
Nuclear Ribosomal Dna ◽  
Minimum Evolution ◽  
West Pacific ◽  
Pyloric Caeca ◽  
Dna Sequence Data

A survey of the parasites of Indo-West Pacific Haemulidae revealed the presence of three new cryptogonimid (Digenea: Cryptogonimidae) species warranting two new genera, Beluesca littlewoodi n. gen., n. sp. and B. longicolla n. sp. from the intestine and pyloric caeca of Plectorhinchus gibbosus and Chelediadema marjoriae n. gen., n. sp. from the intestine and pyloric caeca of Diagramma labiosum, P. albovittatus and P. gibbosus from Heron and Lizard Islands off the Great Barrier Reef, Australia. Beluesca n. gen. is distinguished from all other cryptogonimid genera by the combination of an elongate body, funnel-shaped oral sucker, relatively small number of large oral spines, highly lobed ovary, opposite to slightly oblique testes, uterine loops that are restricted to the hindbody and extend well posterior to the testes and vitelline follicles that may extend from the ovary into the forebody, but do not extend anterior to the intestinal bifurcation. Pseudallacanthochasmus plectorhynchi Mamaev, 1970 is transferred to Beluesca as B. plectorhyncha (Mamaev, 1970) n. comb. based on morphological and ecological (host preference) characteristics. Chelediadema n. gen. is distinguished from all other cryptogonimid genera by the combination of a lanceolate body, relatively small number of large oral spines, prepharynx that is much longer than the oesophagus, tandem testes, uterine loops that are extensive in the hindbody and extend well posterior to the testes and vitelline follicles that extend from the ovary to the pharynx. Morphological analysis of the three species described here was augmented with DNA sequence analyses utilizing data from the large subunit (LSU) and the internal transcribed spacers (ITS) 1 and 2, and 5.8S nuclear ribosomal DNA. Sequence data from the LSU and ITS (encompassing the ITS1, 5.8S and ITS2) of the taxa examined here were aligned with those reported for other cryptogonimids, Caulanus thomasi, Latuterus tkachi, Neometadena ovata, four representative species of Retrovarium and an undescribed species of Siphoderina, for comparative purposes and to explore levels of interspecific and intergeneric variation among these taxa. Minimum evolution analysis was conducted on a combined (LSU and ITS) dataset to explore relationships among these genera. Despite their superficial morphological and host preference similarities, species of Beluesca and Chelediadema were genetically distant from each other. Interspecific and intergeneric variation among the species described here is similar to that reported for other cryptogonimids.

scholarly journals Phylogeny and PCR Identification of Clinically Important Zygomycetes Based on Nuclear Ribosomal-DNA Sequence Data

1999 ◽  
Vol 37 (12) ◽  
pp. 3957-3964 ◽  
Author(s):  
Kerstin Voigt ◽  
Elizabeth Cigelnik ◽  
Kerry O'donnell
Keyword(s):  
Dna Sequence ◽  
Ribosomal Dna ◽  
Dna Sequences ◽  
Sequence Data ◽  
Large Subunit ◽  
Gene Tree ◽  
Strong Support ◽  
Nuclear Ribosomal Dna ◽  
Syncephalastrum Racemosum ◽  
Dna Sequence Data

A molecular database for all clinically important Zygomycetes was constructed from nucleotide sequences from the nuclear small-subunit (18S) ribosomal DNA and domains D1 and D2 of the nuclear large-subunit (28S) ribosomal DNA. Parsimony analysis of the aligned 18S and 28S DNA sequences was used to investigate phylogenetic relationships among 42 isolates representing species of Zygomycetes reported to cause infections in humans and other animals, together with commonly cultured contaminants, with emphasis on members of the Mucorales. The molecular phylogeny provided strong support for the monophyly of the Mucorales, exclusive of Echinosporangium transversale andMortierella spp., which are currently misclassified within the Mucorales. Micromucor ramannianus, traditionally classified within Mortierella, and Syncephalastrum racemosum represent the basal divergences within the Mucorales. Based on the 18S gene tree topology, Absidia corymbiferaand Rhizomucor variabilis appear to be misplaced taxonomically. A. corymbifera is strongly supported as a sister group of the Rhizomucor miehei-Rhizomucor pusillusclade, while R. variabilis is nested withinMucor. The aligned 28S sequences were used to design 13 taxon-specific PCR primer pairs for those taxa most commonly implicated in infections. All of the primers specifically amplified DNA of the size predicted based on the DNA sequence data from the target taxa; however, they did not cross-react with phylogenetically related species. These primers have the potential to be used in a PCR assay for the rapid and accurate identification of the etiological agents of mucormycoses and entomophthoromycoses.

Synonymy of the three Villaria Rolfe species (Rubiaceae): evidence from morphological and nuclear ribosomal DNA sequence data

Acta Manilana ◽  
2010 ◽  
Vol 56 (0) ◽  
Author(s):  
GJD Alejandro ◽  
DLA Arlegui ◽  
PMO Detabali ◽  
EA Espino ◽  
EG Layson ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Ribosomal Dna ◽  
Sequence Data ◽  
Nuclear Ribosomal Dna ◽  
Dna Sequence Data ◽  
Ribosomal Dna Sequence

scholarly journals PCR identification of Fusarium genus based on nuclear ribosomal-DNA sequence data

2003 ◽  
Vol 2 (4) ◽  
pp. 82-85 ◽  
Author(s):  
A. Abd-Elsalam Kamel ◽  
N. Aly Ibrahim ◽  
A. Abdel-Satar Mohmed ◽  
S. Khalil Mohmed ◽  
A. Verreet Joseph
Keyword(s):  
Dna Sequence ◽  
Ribosomal Dna ◽  
Sequence Data ◽  
Nuclear Ribosomal Dna ◽  
Dna Sequence Data ◽  
Pcr Identification ◽  
Ribosomal Dna Sequence

Delayed germination of basidiospores in temperate species of Crepidotus (Fr.) Staude

2002 ◽  
Vol 80 (3) ◽  
pp. 280-287 ◽  
Author(s):  
M C Aime ◽  
O K Miller Jr.
Keyword(s):  
Dna Sequence ◽  
Sequence Data ◽  
Abiotic Factors ◽  
Large Subunit ◽  
Early Spring ◽  
Single Spore ◽  
Dna Sequence Data ◽  
Delayed Germination ◽  
First Time ◽  
Ribosomal Dna Sequence

Delayed basidiospore germination (endogenous dormancy) for a number of species of Crepidotus (Agaricales: Crepidotaceae) is reported for the first time. Ninety percent of recovered single spore isolates germinated between 18 and 36 weeks after collection; average germination was 25 weeks after collection. The period in which 90% of germinations occurred was between 17 February and 19 April, with 50% of recovered isolates germinating in March. Many abiotic factors were experimentally manipulated in an effort to reduce or alter the necessary incubation period without effect. The latent period was consistent for a given collection, with the majority of recovered isolates from fall-fruiting collections germinating during early spring, regardless of whether spores were plated immediately after harvesting or stored for one to several months prior to plating. The identity of the cultures derived from delayed germination was confirmed by DNA sequencing.Key words: basidiospore, dormancy, single spore isolates, Crepidotaceae, large subunit ribosomal DNA sequence data, phylogeny.

Phylogeny and biogeography of the genus Hesperis (Brassicaceae, tribe Hesperideae) inferred from nuclear ribosomal DNA sequence data

2021 ◽  
Vol 307 (2) ◽  
Author(s):  
Atena Eslami-Farouji ◽  
Hamed Khodayari ◽  
Mostafa Assadi ◽  
Özlem Çetin ◽  
Klaus Mummenhoff ◽  
...  
Keyword(s):  
Dna Sequence ◽  
Ribosomal Dna ◽  
Sequence Data ◽  
Nuclear Ribosomal Dna ◽  
Dna Sequence Data ◽  
Ribosomal Dna Sequence

scholarly journals Three new species of Fomitiporella (Hymenochaetales, Basidiomycota) based on the evidence from morphology and DNA sequence data

MycoKeys ◽  
2018 ◽  
Vol 30 ◽  
pp. 73-89 ◽  
Author(s):  
Xiao-Hong Ji ◽  
Josef Vlasák ◽  
Xue-Mei Tian ◽  
Yu-Cheng Dai
Keyword(s):  
New Species ◽  
Dna Sequence ◽  
Sequence Data ◽  
Large Subunit ◽  
Its Region ◽  
Morphological Characters ◽  
White Rot ◽  
Molecular Evidence ◽  
Dna Sequence Data ◽  
Three New Species

Fomitiporella austroasiana, F. mangrovei and F. vietnamensis are described and illustrated as new species based on morphological characters and molecular evidence. They have annual to perennial, mostly resupinate basidiomata with grayish fresh pores, an indistinct subiculum, lack any kind of setae, have brownish, thick-walled basidiospores, and cause a white rot. The distinctive morphological characters of the new species and their related species are discussed. Phylogenies based on the nuclear ribosomal large subunit (28S) and the nuclear ribosomal ITS region show that these three new species form three distinct lineages in the Fomitiporella clade. A key to known species of Fomitiporella is given.

Comparison of Puccinia acroptili from Eurasia and the USA

Botany ◽  
2012 ◽  
Vol 90 (6) ◽  
pp. 465-471 ◽  
Author(s):  
W.L. Bruckart ◽  
F.M. Eskandari ◽  
D.K. Berner ◽  
M.C. Aime
Keyword(s):  
Dna Sequence ◽  
Sequence Data ◽  
Large Subunit ◽  
Single Species ◽  
The United States ◽  
Rust Disease ◽  
Dna Sequence Data ◽  
Russian Knapweed ◽  
The Usa ◽  
Recent Evaluation

A rust disease caused by Puccinia acroptili P. Syd. & Syd. occurs throughout the range of Russian knapweed (Rhaponticum (syn. Acroptilon) repens), including North America. Differences in teliospore dimensions had been observed previously, but not quantified, between a specimen of P. acroptili from Turkey and one from the United States of America (USA). Similar differences were found during a recent evaluation of P. acroptili for biological control of R. repens in the USA; e.g., teliospores from eight USA isolates were 2.4 µm shorter (P = 0.05) than four isolates from Eurasia (two from Turkey, and one each from Russia and Kazakhstan). This inspired the generation of additional biological and DNA sequence data to clarify the significance of these differences between USA and Eurasian isolates. Although the USA isolates were found to have shorter teliospores, as noted in the description by Savile, teliospores of the USA isolates were also significantly wider in diameter than isolates from Eurasia; the latter in contrast to Savile’s observation. Biologically, the isolates were the same; all were equally aggressive in causing disease under common greenhouse test conditions, and fertile crosses occurred between isolates regardless of source. DNA sequence analyses of the nuclear rDNA large subunit and internal transcribed spacer regions supported the notion that P. acroptili is a single species, but it also revealed that small levels of variation may exist within the species. For these reasons, it has been concluded that USA and Eurasian accessions can be considered a single species, i.e., P. acroptili. Also, Savile’s observation about differences in spore dimensions is substantiated.

scholarly journals Phylogeny of the family Trogidae (Coleoptera: Scarabaeoidea) inferred from mitochondrial and nuclear ribosomal DNA sequence data

2014 ◽  
Vol 39 (3) ◽  
pp. 548-562 ◽  
Author(s):  
WERNER P. STRÜMPHER ◽  
CATHERINE L. SOLE ◽  
MARTIN H. VILLET ◽  
CLARKE H. SCHOLTZ
Keyword(s):  
Dna Sequence ◽  
Ribosomal Dna ◽  
Sequence Data ◽  
Nuclear Ribosomal Dna ◽  
Dna Sequence Data ◽  
The Family ◽  
Ribosomal Dna Sequence

DISTINCTION BETWEEN CRINUM LATIFOLIUM AND CRINUM ASIATICUM (AMARYLLIDACEAE) USING MOLECULAR DATA

2020 ◽  
Vol 27 (03) ◽  
Author(s):  
VAN HONG THIEN ◽  
TRAN THỊ THU NGAN ◽  
LUU THAO NGUYEN ◽  
TRINH NGOC NAM
Keyword(s):  
Phylogenetic Tree ◽  
Dna Sequence ◽  
Internal Transcribed Spacer ◽  
Sequence Data ◽  
Morphological Characteristics ◽  
Molecular Data ◽  
Nuclear Ribosomal Dna ◽  
Dna Sequence Data ◽  
Crinum Asiaticum ◽  
Molecular Makers

Crinum latifolium and C. asiaticum are the two species of genus Crinum, Amaryllidaceae family and they have highly medicinal values. Distinction between two species is challenging due to many similar morphological characteristics. In this study, we used molecular makers to distinguish the two species Crinum latifolium and C. asiaticum. The phylogenetic tree was constructed based on the DNA sequence data of the ITS (internal transcribed spacer) nuclear ribosomal DNA (nrDNA) sequences of Crinum latifolium and C. asiaticum. As a result, C. latifolium and C. asiaticum were sorted into two different groups in the phylogenetic tree. Therefore, C. latifolium and C. asiaticum were carried out to solve the taxonomic ambiguity.

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