A new Epeorus (Caucasiron) (Ephemeroptera: Heptageniidae) species from Turkey based on molecular and morphological evidence

Zootaxa ◽  
2019 ◽  
Vol 4550 (1) ◽  
pp. 58 ◽  
Author(s):  
ĽUBOŠ HRIVNIAK ◽  
PAVEL SROKA ◽  
GENCER TÜRKMEN ◽  
ROMAN J. GODUNKO ◽  
NİLGÜN KAZANCI
Keyword(s):  
New Species ◽  
Morphological Analysis ◽  
Distinct Species ◽  
Individual Species ◽  
Morphological Evidence ◽  
Fine Hair ◽  
The Caucasus ◽  
Molecular Dataset ◽  
Coi Sequences ◽  
Analytical Approaches

Epeorus (Caucasiron) turcicus sp. nov. is described based on larvae from NE Turkey. The new species can be distinguished from other Caucasiron by a unique combination of several diagnostic characters: the presence of a rounded hypodermal medial femur spot, colouration of abdominal terga and sterna, narrow gill plate VII, fine hair-like setae on the surface of abdominal terga, and absence of postero-lateral projections on tergum X. In addition to morphological analysis, two single-locus analytical approaches are employed for delimiting the new species using COI sequences (Automatic Barcode Gap Discovery, ABGD; and General Mixed Yule Coalescent Model, GMYC). Both approaches unambiguously recognized E. (C.) turcicus sp. nov. as a distinct species. Our molecular dataset contains all Caucasiron species occurring in the Caucasus and the delimitation of individual species mostly follows the morphologically defined species. This study confirms the suitability of the GMYC approach for species delimitation within Caucasiron. 

A new crenobiontic water mite species of the genus Atractides Koch, 1837 from Montenegro and Bulgaria, based on morphological and molecular data (Acariformes, Hydrachnidia, Hygrobatidae)

2020 ◽  
Vol 25 (10) ◽  
pp. 1889-1900
Author(s):  
Vladimir Pešić ◽  
Andrzej Zawal ◽  
Aleksandra Bańkowska ◽  
Milica Jovanović ◽  
Miroslawa Dabert
Keyword(s):  
New Species ◽  
Morphological Analysis ◽  
Molecular Data ◽  
Mite Species ◽  
The Novel ◽  
Molecular Tools ◽  
Mtdna Coi ◽  
Coi Sequences ◽  
Morphological And Molecular Data ◽  
A New Species

A new species of water mites from Montenegro and Bulgaria, Atractides anae Pešić sp. nov., is described, combining traditional morphological techniques with the analysis of partial mtDNA COI sequences (DNA-barcodes). Based on our molecular and morphological analysis, the new species is most closely related to the crenobiontic A. pennatus (K. Viets 1920) from which it differs by 6.4 (SD = 1.12)% K2P distance. A dataset including the novel COI sequences of 10 Atractides species from Montenegro and Iran is assembled in order to facilitate further studies on the genus by using molecular tools.

scholarly journals Identification of the taxonomic status of Scinax nebulosus and Scinax constrictus (Scinaxinae, Anura) based on molecular markers

2020 ◽  
Author(s):  
T. M. B. Freitas ◽  
J. B. L. Sales ◽  
I. Sampaio ◽  
N. M. Piorski ◽  
L. N. Weber
Keyword(s):  
Molecular Markers ◽  
Morphological Traits ◽  
Morphological Analysis ◽  
Taxonomic Status ◽  
Distinct Species ◽  
Brazilian Cerrado ◽  
Anuran Species ◽  
Cerrado Savanna ◽  
Statistical Support ◽  
Analytical Approaches

Abstract The validation of many anuran species is based on a strictly descriptive, morphological analysis of a small number of specimens with a limited geographic distribution. The Scinax Wagler, 1830 genus is a controversial group with many doubtful taxa and taxonomic uncertainties, due a high number of cryptic species. One example is the pair of species Scinax constrictus and Scinax nebulosus, which share a similar morphology. Scinax constrictus is restricted to the Brazilian Cerrado savanna, while S. nebulosus is widely distributed throughout northern South America. Despite the validation of many anuran species, discriminations based only on morphological traits is quite difficult due to the high conservative morphology of some groups. In this context, the present study uses mitochondrial and nuclear genes to provide a more consistent diagnosis and test the validity of S. constrictus as a distinct species from S. nebulosus, as well as evaluate the position of these taxa within the Scinax genus. The topologies obtained herein uphold the monophyletic status of Scinax based on all molecular markers assessed in this study, in all analytical approaches, with high levels of statistical support.

Karyotypic Evolution in Gehyra (Gekkonidae: Reptilia) II.* A New Species from the Alligator Rivers Region in Northern Australia

1982 ◽  
Vol 30 (1) ◽  
pp. 93 ◽  
Author(s):  
M King
Keyword(s):  
New Species ◽  
Morphological Analysis ◽  
Distinct Species ◽  
Northern Territory ◽  
Chromosomal Analysis ◽  
Karyotypic Evolution ◽  
Northern Australia ◽  
Arnhem Land ◽  
Human Habitation ◽  
A New Species

A chromosomal analysis of gekkos of the Gehyra australis complex from the Alligator Rivers region of the Northern Territory indicates that those animals living on trees or human habitation have 2n=40 chromosomes, whereas those living on the rock outliers and cliffs of the Arnhem Land escarpment have 2n=42. A morphological analysis of these specimens shows that two distinct species are present: Gehyra australis and a new species, which is described below as Gehyra pamela.

Genetic and morphological evidence for a new cryptic species of Ectinogonia (Coleoptera: Buprestidae) from central Chile

Zootaxa ◽  
2017 ◽  
Vol 4303 (2) ◽  
pp. 284 ◽  
Author(s):  
SIMÓN ANGUITA-SALINAS ◽  
RODRIGO M. BARAHONA-SEGOVIA ◽  
ELIE POULIN ◽  
ALVARO ZÚÑIGA-REINOSO
Keyword(s):  
New Species ◽  
Genetic Distance ◽  
Cryptic Species ◽  
Distance Matrix ◽  
Morphological Evidence ◽  
Mountain Range ◽  
Central Chile ◽  
The Andes ◽  
Genetic Distance Matrix ◽  
Coi Sequences

The genus Ectinogonia Spinola, 1837 is a genus mainly found in Chile; it currently contains 17 species. Recent exploration in the Andes Mountain Range of the Bio Bio Region in Chile have resulted in the collection of specimens slightly different morphologically from all previously described species. The aim of this paper is to describe this new species of Ectinogonia using morphological and genetic evidence. To establish differences between species we described the external morphology and compared it to species that are morphologically similar (i.e. E. buqueti Spinola 1837 and E. intermedia Kerremans 1903). We also measured the genetic differences in COI sequences, constructing a distance matrix in which we compared it to species that are morphologically similar (E. buqueti and E. intermedia) and other species found in the same region (E. speciosa oscuripennis Moore 1994). We found that E. cryptica sp. n. differs from E. buqueti (which previously contained E. cryptica sp. n.) in pronotum and elytral patterns. The genetic distance matrix shows that E. cryptica sp. n. differs by 4.6% from all other Ectinogonia species compared, supporting the morphological evidence. 

scholarly journals Mideopsis milankovici sp. nov. a new water mite from Montenegro based on morphological and molecular data (Acariformes, Hydrachnidia, Mideopsidae)

Acarologia ◽  
2020 ◽  
Vol 60 (3) ◽  
pp. 566-575
Author(s):  
Vladimir Pešić ◽  
Harry Smit
Keyword(s):  
New Species ◽  
Morphological Characteristics ◽  
Distinct Species ◽  
Molecular Data ◽  
Mite Species ◽  
Water Mite ◽  
Coi Sequences ◽  
Taxonomic Approach ◽  
Morphological And Molecular Data ◽  
Standing Waters

Water mite species of the genus Mideopsis Neuman, 1880 are common in running and standing waters in the Palaearctic. In the present study we used an integrative taxonomic approach by applying partial COI sequences (DNA-barcodes) and morphological characteristics to describe a new species, Mideopsis milankovici sp. nov. from the Mediterranean region of Montenegro. A high genetic distance (18.8-26% K2P) from three other known European congeners, M. crassipes Soar, 1904, M. orbicularis (Müller, 1776), and M. roztoczensis Biesiadka and Kowalik, 1979, support M. milankovici sp. nov. as a distinct species. From M. persicus Pešić and Saboori, 2015, a species known from South Iran, which resembles the new species in the shape of the ejaculatory complex, M. milankovici sp. nov. differs by the morphology of dorsal shield.

Phylogeny and molecular species delimitation of long-nosed armadillos (Dasypus: Cingulata) supports morphology-based taxonomy

2019 ◽  
Vol 186 (3) ◽  
pp. 813-825 ◽  
Author(s):  
Anderson Feijó ◽  
Júlio F Vilela ◽  
Jilong Cheng ◽  
Marco Antônio A Schetino ◽  
Raphael T F Coimbra ◽  
...  
Keyword(s):  
Molecular Species ◽  
Species Delimitation ◽  
Distinct Species ◽  
Molecular Data ◽  
Species Boundaries ◽  
Morphological Evidence ◽  
Guiana Shield ◽  
Taxonomic History ◽  
Molecular Dataset ◽  
Phylogenetic Hypotheses

AbstractThe armadillo genus Dasypus is the most species-rich and widely distributed genus of the order Cingulata and it has a dynamic taxonomic history. Recent morphology-based studies have proposed new taxonomic arrangements, but these were not yet assessed with molecular data. The two comprehensive phylogenetic hypotheses available for the genus are conflicting and were each based on a subset of taxa, hampering a proper evaluation of species boundaries. Using a multilocus molecular dataset, based on the broadest geographic sampling of Dasypus to date, we inferred the phylogenetic relationships of all species of the genus, including the recently reinstated D. beniensis and D. pastasae. We tested recent taxonomic hypotheses using several species-delimitation approaches. Our phylogeny recovered three main lineages of long-nosed armadillos that we treat as subgenera (Hyperoambon, Muletia and Dasypus) and identified the majority of its diversification as having occurred during the Pliocene. Molecular species delimitation supported morphological evidence in assigning D. hybridus as a subspecies of D. septemcinctus and confirming the split of the D. kappleri complex into three species. Our results strongly support the recognition of Guiana Shield populations formerly assigned to D. novemcinctus as a distinct species. The phylogenetic positions of D. mazzai and D. sabanicola remain uncertain. Further investigation using faster-evolving genes and additional samples may help to clarify the relationships of these young species.

scholarly journals Dipteris shenzhenensis, a new endangered species of Dipteridaceae from Shenzhen, southern China

PhytoKeys ◽  
2021 ◽  
Vol 186 ◽  
pp. 111-120
Author(s):  
Zuo-Ying Wei ◽  
Yu-Feng Gu ◽  
Zeng-Qiang Xia ◽  
Li-Jun Chen ◽  
Ting Wang ◽  
...  
Keyword(s):  
New Species ◽  
Statistical Analysis ◽  
Endangered Species ◽  
Quantitative Trait ◽  
Southern China ◽  
Distinct Species ◽  
Morphological Evidence ◽  
Molecular Evidence ◽  
A New Species

Dipteris shenzhenensis, a new species of ferns from Shenzhen, Guangdong, southern China, is identified and described. It closely resembles D. chinensis but possesses several unique traits, such as long rhizome scales, castaneous stipe, and abaxially pale fronds with two fan-shaped fronds connected by a broad wing. Molecular evidence showed that D. shenzhenensis is allied to D. conjugata, whereas it has morphologically significant differences (P < 0.05) on the basis of quantitative trait statistical analysis. Overall, the morphological evidence, taken together with the result of cpDNA indicated that D. shenzhenensis is a distinct species.

scholarly journals A new species of Homonota (Squamata: Gekkota: Phyllodactylidae) from Paraguay

Holotipus ◽  
2021 ◽  
Vol 2 (2) ◽  
Author(s):  
Hugo Cabral ◽  
◽  
Pier Cacciali ◽  
Keyword(s):  
New Species ◽  
South America ◽  
Phylogenetic Relationships ◽  
Morphological Analysis ◽  
Dna Analysis ◽  
Morphological Evidence ◽  
Southern South America ◽  
Comparative Morphological Analysis ◽  
Rocky Outcrops ◽  
A New Species

The 12 recognized species of the genus Homonota are geographically restricted to central and southern South America in Argentina, Brazil, Uruguay, Bolivia, and Paraguay. The phylogenetic relationships within the genus are well known where three groups are identified, each of them containing four species: the whitii group (Homonota darwinii, H. williamsii, H. whitii, and H. andicola), the borellii group (H. borellii, H. taragui, H. rupicola, and H. uruguayensis) and the horrida group (H. horrida, H. underwoodi, H. septentrionalis, and H. marthae). During fieldwork in Paraguay, we found a specimen of Homonota with unique morphological and geographical traits. Thus, we performed a comparative morphological analysis against specimens housed in different Paraguayan collections. Additionally, we performed a DNA analysis of our specimen to assess its relationships within the genus. Results suggest that this specimen represents a new species of the Homonota horrida group based on molecular and morphological evidence. The new species is characterized by a robust body, 10 longitudinal rows of strongly keeled scales separated by one or two granular scales, square-shaped mental scales, small postmental scales, one or two rows of rectangular scales in the upper eye scales, rostral scale divided, edge of auditory meatus serrated posteriorly, and black and brown background coloration with one white vertebral line and seven white transverse bands. The new species is restricted to Paraguarí Department, characterized by herbaceous grasslands with rocky outcrops.

Molecular and morphological evidence for two new species of terrestrial planarians of the genus Microplana (Platyhelminthes; Turbellaria; Tricladida; Terricola) from Europe

Zootaxa ◽  
2008 ◽  
Vol 1945 (1) ◽  
pp. 1-38 ◽  
Author(s):  
HUGH D. JONES ◽  
BONNIE L. WEBSTER ◽  
D. TIMOTHY J. LITTLEWOOD ◽  
Jillian C. Mcdonald
Keyword(s):  
New Species ◽  
Distinct Species ◽  
Type Material ◽  
Morphological Evidence ◽  
Size And Shape ◽  
Copulatory Apparatus ◽  
Two New Species

Three species of terrestrial planarian were found in a garden in Yorkshire, Microplana scharffi (Graff 1896), M. terrestris (Müller 1774) and a third Microplana species similar in size and shape to M. terrestris but brown (khaki) rather than grey/black. Mitochondrial cytochome oxidase I sequences show that the three are distinct species, but that the sequences of the khaki specimens are identical with a GenBank sequence under the name “Microplana terrestris”. Photomicrographs of the copulatory apparatus of several specimens of each species, including type material, are presented which confirm that the three species are distinct and show the variation within each species. The khaki species is named as Microplana kwiskea n. sp. Specimens from Spain relating to the GenBank “Microplana terrestris” sequence have been examined and are not of that species, nor are they M. kwiskea. They are considered to be a further species, M. groga n. sp.

Earning your stripes: a second species of striped gecko in the New Zealand gecko genus Toropuku (Gekkota: Diplodactylidae)

Zootaxa ◽  
2020 ◽  
Vol 4890 (4) ◽  
pp. 578-588
Author(s):  
RODNEY A. HITCHMOUGH ◽  
STUART V. NIELSEN ◽  
AARON M. BAUER
Keyword(s):  
New Species ◽  
New Zealand ◽  
Conservation Status ◽  
Distinct Species ◽  
Species Level ◽  
Morphological Evidence ◽  
Evidence Based ◽  
Cook Strait ◽  
North And South ◽  
A New Species

The New Zealand diplodactylid gecko genus Toropuku is currently monotypic, but the sole member of the genus, T. stephensi, is distributed in two disjunct, geographically distant regions of New Zealand – the islands of Cook Strait (which includes the type locality, Stephens Island), between New Zealand’s North and South Islands, and the Coromandel Peninsula, in the northeastern North Island. Previously published phylogenetic results, based on three total individuals, recognized substantial—possibly species-level—diversity between these disparate localities, although no taxonomic decisions were made at that time. More recently, additional animals have been found on the Coromandel Peninsula. We here present phylogenetic and morphological evidence based on this expanded dataset to formally describe the populations on the Coromandel Peninsula as a new species, Toropuku inexpectatus sp. nov. The specific epithet refers to the species’ surprise discovery in a herpetologically well-surveyed area. The recognition of T. inexpectatus sp. nov. as a distinct species has implications for the conservation status of T. stephensi, which is now considered restricted to three islands in Cook Strait. 

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