COI mtDNA barcoding and morphology for species delimitation in the spider genus Ixchela Huber (Araneae: Pholcidae), with the description of two new species from Mexico

Zootaxa ◽  
2020 ◽  
Vol 4747 (1) ◽  
pp. 54-76
Author(s):  
ALEJANDRO VALDEZ-MONDRAGÓN
Keyword(s):  
New Species ◽  
Central America ◽  
Species Delimitation ◽  
Mitochondrial Cytochrome ◽  
Neighbor Joining ◽  
Molecular Analyses ◽  
North Eastern ◽  
Two New Species ◽  
Males And Females ◽  
Barcode Gap

The spider genus Ixchela Huber 2000, is comprised of 22 species distributed from north-eastern Mexico to Central America, including the two new species described herein from Mexico: Ixchela panchovillai sp. nov. and Ixchela zapatai sp. nov., both from the state of Oaxaca and described for both sexes. DNA barcoding utilizing mitochondrial cytochrome c oxidase subunit 1 (COI) and morphology were used for species delimitation. Molecular analyses and species delimitation included four methods: 1) corrected p-distances under neighbor-joining (NJ), 2) general mixed yule coalescent model (GMYC), 3) automatic barcode gap discovery (ABGD), and 4) Poisson tree processes (bPTP). All molecular methods and morphology were consistent in delimiting and recognizing the two new species describing herein. The average inter-specific genetic distance (p-distance) within the genus Ixchela is 12%. Ixchela panchovillai sp. nov. is closely related in the NJ analysis with I. placida, with an average p-distance of 7.9%, whereas I. zapatai sp. nov. is closely related to I. taxco, with an average p-distance of 8.4% between both species. Additionally, identification keys for males and females of the genus Ixchela are presented. 

scholarly journals COI mtDNA barcoding and morphology for the description of a new species of ricinuleid of the genus Pseudocellus (Arachnida: Ricinulei: Ricinoididae) from El Triunfo Biosphere Reserve, Chiapas, Mexico

2021 ◽  
Vol 778 ◽  
Author(s):  
Alejandro Valdez-Mondragón ◽  
Mayra R. Cortez-Roldán
Keyword(s):  
New Species ◽  
Species Delimitation ◽  
Biosphere Reserve ◽  
Mitochondrial Cytochrome ◽  
Adult Males ◽  
Molecular Analyses ◽  
Interspecific Genetic Distance ◽  
Males And Females ◽  
A New Species ◽  
Barcode Gap

A new species of epigean ricinuleid of the genus Pseudocellus Platnick, 1980 from El Triunfo Biosphere Reserve, Chiapas, Mexico is described. DNA barcoding utilizing mitochondrial cytochrome c oxidase subunit 1 (CO1) and morphology were used for species delimitation. Molecular analyses and species delimitation included four methods: 1) General Mixed Yule Coalescent model (GMYC), 2) Automatic Barcode Gap Discovery (ABGD), 3) Bayesian Poisson Tree Process (bPTP), and 4) Assemble Species by Automatic Partitioning (ASAP). All molecular methods and morphology were consistent in delimiting and recognizing the new species described herein. The average interspecific genetic distance (p-distance) among analyzed species of Pseudocellus was 11.6%. The species is described based on adult males and females: Pseudocellus giribeti sp. nov. This is the seventh species described from Chiapas, which holds the highest number of ricinuleids species for the country. The total number of described species of Pseudocellus from Mexico increases to 21, having the highest species diversity of known ricinuleids worldwide.

scholarly journals Revision of the species of Lytopylus from Area de Conservación Guanacaste, northwestern Costa Rica (Hymenoptera, Braconidae, Agathidinae)

ZooKeys ◽  
2017 ◽  
Vol 721 ◽  
pp. 93-158 ◽  
Author(s):  
Ilgoo Kang ◽  
Eric G. Chapman ◽  
Daniel H. Janzen ◽  
Winnie Hallwachs ◽  
Tanya Dapkey ◽  
...  
Keyword(s):  
Species Delimitation ◽  
Dna Barcode ◽  
Mitochondrial Cytochrome ◽  
Neighbor Joining ◽  
Ecological Information ◽  
Dichotomous Key ◽  
Two New Species ◽  
Gene Coi ◽  
Collection Date ◽  
I Gene

Thirty two new species of Lytopylus (Agathidinae) are described with image plates for each species: Lytopylus alejandromasisisp. n., Lytopylus alfredomainierisp. n., Lytopylus anamariamongeaesp. n., Lytopylus angelagonzalezaesp. n., Lytopylus cesarmoraisp. n., Lytopylus eddysanchezisp. n., Lytopylus eliethcantillanoaesp. n., Lytopylus ericchapmanisp. n., Lytopylus gahyunaesp. n., Lytopylus gisukaesp. n., Lytopylus guillermopereiraisp. n., Lytopylus gustavoinduniisp. n., Lytopylus hartmanguidoisp. n., Lytopylus hernanbravoisp. n., Lytopylus hokwonisp. n., Lytopylus ivanniasandovalaesp. n., Lytopylus johanvalerioisp. n., Lytopylus josecortesisp. n., Lytopylus luisgaritaisp. n., Lytopylus mariamartachavarriaesp. n., Lytopylus miguelviquezisp. n., Lytopylus motohasegawaisp. n., Lytopylus okchunaesp. n., Lytopylus pablocobbisp. n., Lytopylus robertofernandezisp. n., Lytopylus rogerblancoisp. n., Lytopylus salvadorlopezisp. n., Lytopylus sangyeonisp. n., Lytopylus sarahmeierottoaesp. n., Lytopylus sergiobermudezisp. n., Lytopylus sigifredomarinisp. n., and Lytopylus youngcheaesp. n. A dichotomous key and a link to an electronic, interactive key are included. All specimens were reared from Lepidoptera larvae collected in Area de Conservación Guanacaste (ACG) and all are associated with ecological information including host caterpillar, collection date, eclosion date, caterpillar food plant, and locality. Neighbor-joining and maximum likelihood analyses of the barcode region of the mitochondrial cytochrome c oxidase subunit I gene (COI DNA barcode) were conducted to aid in species delimitation.

scholarly journals Molecular and morphological analyses confirm two new species of the Hydraena emarginata–saga clade (Coleoptera, Hydraenidae) from Spain and France

Zootaxa ◽  
2011 ◽  
Vol 2760 (1) ◽  
pp. 29 ◽  
Author(s):  
MARCO TRIZZINO ◽  
MANFRED A. JÄCH ◽  
PAOLO AUDISIO ◽  
IGNACIO RIBERA
Keyword(s):  
New Species ◽  
Geographic Range ◽  
Undescribed Species ◽  
Central Spain ◽  
North Central ◽  
Molecular Analyses ◽  
North Eastern ◽  
Two New Species

Using morphological and molecular analyses, the existence of two undescribed species, H. diazi from north–eastern Spain and French Pyrenees, and H. fosterorum from north–central Spain is confirmed. These species are members of a European endemic complex of hydraenid beetles, the Hydraena emarginata–saga clade, belonging to the ”Haenydra“ lineage. The two new species are described and the geographic range of the widespread H. saga is revised.

Species boundaries among barred river frogs, Mixophyes (Anura: Myobatrachidae) in north-eastern Australia, with descriptions of two new species

Zootaxa ◽  
2006 ◽  
Vol 1228 (1) ◽  
pp. 35 ◽  
Author(s):  
MICHAEL MAHONY ◽  
STEPHEN C. DONNELLAN ◽  
STEPHEN J. RICHARDS ◽  
KEITH MCDONALD
Keyword(s):  
Mitochondrial Dna ◽  
New Species ◽  
Species Boundaries ◽  
Molecular Analyses ◽  
Eastern Australia ◽  
North Eastern ◽  
Two New Species ◽  
Wet Tropics ◽  
World Heritage Area ◽  
Heritage Area

Mixophyes are large ground-dwelling myobatrachid frogs from eastern Australia and New Guinea. We use analyses of allozyme frequencies, nucleotide sequences of mitochondrial DNA and morphology to define species boundaries in Mixophyes from the Wet Tropics World Heritage Area of northern Queensland. The molecular analyses identify a minimum of three species in the region. Morphometric and meristic analyses corroborate these distinctions. The existence of two of these species was not previously suspected, and they are formally described herein.

Biodiversity in the Andean Mountains: Two new rain frogs of the genus Pristimantis (Anura: Craugastoridae) from the northern Cordillera Central in Colombia

Zootaxa ◽  
2021 ◽  
Vol 5040 (3) ◽  
pp. 334-364
Author(s):  
MAURICIO RIVERA-CORREA ◽  
GUSTAVO A. GONZÁLEZ-DURÁN ◽  
ANA M. SALDARRIAGA-GÓMEZ ◽  
SEBASTIÁN DUARTE-MARÍN
Keyword(s):  
New Species ◽  
Species Delimitation ◽  
Intraspecific Diversity ◽  
Conservation Strategies ◽  
Phylogenetic Position ◽  
The Andes ◽  
Two New Species ◽  
Diversity Studies ◽  
First Time ◽  
Central Cordillera

Frequent biodiversity sampling and monitoring programs often lead to relevant taxonomic findings. Here, as a product of different field expeditions to two places in the northern Andes, we discover and describe two new species of rain frogs of the genus Pristimantis from the cloud forests of the Central Cordillera of Colombia. Pristimantis chocolatebari sp. nov. is diagnosed by having the following character states: dentigerous process of vomer triangular, snout large and protruding, dorsum smooth, vocal slits and vocal sac present, groin and hidden part of the thighs yellow. Pristimantis carylae sp. nov. is diagnosed by having iris copper red, dentigerous process of vomer oval, snout short and rounded, dorsum finely shagreen with scattered tubercles, double nuptial pad in males, groin and hidden part of thighs pink. We also infer, for the first time, the phylogenetic position of P. permixtus, P. platychilus, and two candidate new species. In addition, we emphasize not to use genetic distance as the only source of evidence for species delimitation, considering the high intraspecific diversity found in one of the species described here. Finally, we highlight the relevance of different habitat and ecosystem conservation strategies to promote amphibian diversity studies in the Andes.  

scholarly journals Two new species of the feather mite genus Analges Nitzsch, 1818 (Analgoidea: Analgidae) from accentors (Passeriformes: Prunellidae) — morphological descriptions with DNA barcode data

2018 ◽  
Vol 23 (12) ◽  
pp. 2288 ◽  
Author(s):  
Jacek Dabert ◽  
Serge V. Mironov ◽  
Marián Janiga
Keyword(s):  
New Species ◽  
Species Delimitation ◽  
Dna Barcode ◽  
Feather Mites ◽  
Feather Mite ◽  
Two New Species ◽  
Mite Genus

Two new species of the genus Analges (Astigmata: Analgoidea) are described from two species of accentors (Passeriformes: Prunellidae): Analges himalayanus sp. nov. from the Altai Accentor P. himalayana (Blyth), and A. slovakiensis sp. nov. from the Alpine Accentor Prunella collaris (Scopoli). Both species are closely related to A. bidentatus Giebel, 1871 described from the Dunnock Prunella modularis (Linnaeus). We extended the standard morphological descriptions of feather mites by species delimitation analyses carried out on DNA barcode sequences.

scholarly journals Studies on neotropical Phasmatodea XXII: Two new species of Taraxippus (Phasmatodea: Cladomorphinae: Hesperophasmatini) and the first record of the genus from Central America

2020 ◽  
Vol 29 (1) ◽  
pp. 101-114
Author(s):  
Oskar V. Conle ◽  
Frank H. Hennemann ◽  
Pablo Valero
Keyword(s):  
New Species ◽  
Costa Rica ◽  
Dominican Republic ◽  
Central America ◽  
First Record ◽  
Distributional Pattern ◽  
Distribution Map ◽  
Two New Species ◽  
First Time

Two new species of Taraxippus Moxey, 1971 are described and illustrated: T. samaraesp. nov. from Costa Rica and Panama and T. perezgelabertisp. nov. from the Dominican Republic. Both sexes and the previously unknown eggs are described. The genus is recorded from Central America for the first time. A distribution map and a discussion of the distributional pattern of Taraxippus are provided.

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