An integrative approach to species delimitation in Echinodorus (Alismataceae) and the description of two new species

Frequent biodiversity sampling and monitoring programs often lead to relevant taxonomic findings. Here, as a product of different field expeditions to two places in the northern Andes, we discover and describe two new species of rain frogs of the genus Pristimantis from the cloud forests of the Central Cordillera of Colombia. Pristimantis chocolatebari sp. nov. is diagnosed by having the following character states: dentigerous process of vomer triangular, snout large and protruding, dorsum smooth, vocal slits and vocal sac present, groin and hidden part of the thighs yellow. Pristimantis carylae sp. nov. is diagnosed by having iris copper red, dentigerous process of vomer oval, snout short and rounded, dorsum finely shagreen with scattered tubercles, double nuptial pad in males, groin and hidden part of thighs pink. We also infer, for the first time, the phylogenetic position of P. permixtus, P. platychilus, and two candidate new species. In addition, we emphasize not to use genetic distance as the only source of evidence for species delimitation, considering the high intraspecific diversity found in one of the species described here. Finally, we highlight the relevance of different habitat and ecosystem conservation strategies to promote amphibian diversity studies in the Andes.

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Two new species of the feather mite genus Analges Nitzsch, 1818 (Analgoidea: Analgidae) from accentors (Passeriformes: Prunellidae) — morphological descriptions with DNA barcode data

Systematic and Applied Acarology ◽

10.11158/saa.23.12.2 ◽

2018 ◽

Vol 23 (12) ◽

pp. 2288 ◽

Cited By ~ 2

Author(s):

Jacek Dabert ◽

Serge V. Mironov ◽

Marián Janiga

Keyword(s):

New Species ◽

Species Delimitation ◽

Dna Barcode ◽

Feather Mites ◽

Feather Mite ◽

Two New Species ◽

Mite Genus

Two new species of the genus Analges (Astigmata: Analgoidea) are described from two species of accentors (Passeriformes: Prunellidae): Analges himalayanus sp. nov. from the Altai Accentor P. himalayana (Blyth), and A. slovakiensis sp. nov. from the Alpine Accentor Prunella collaris (Scopoli). Both species are closely related to A. bidentatus Giebel, 1871 described from the Dunnock Prunella modularis (Linnaeus). We extended the standard morphological descriptions of feather mites by species delimitation analyses carried out on DNA barcode sequences.

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An integrated approach to delimiting species borders in the genus Chrysotoxum Meigen, 1803 (Diptera: Syrphidae), with description of two new species

Contributions to Zoology ◽

10.1163/18759866-08404002 ◽

2015 ◽

Vol 84 (4) ◽

pp. 285-304 ◽

Cited By ~ 17

Author(s):

Zorica Nedeljković ◽

Jelena Ačanski ◽

Mihajla Đan ◽

Dragana Obreht-Vidaković ◽

Antonio Ricarte ◽

...

Keyword(s):

New Species ◽

Integrated Approach ◽

Integrative Taxonomy ◽

Species Boundaries ◽

Integrative Approach ◽

Taxonomic Study ◽

Additional Species ◽

Two New Species ◽

Lateral Margins ◽

A New Species

Integrative taxonomy tests the validity of taxa using methods additional to traditional morphology. The existence of two different morphotypes in specimens identified as Chrysotoxum vernale Loew (Diptera: Syrphidae) prompted their taxonomic study using an integrative approach that included morphology, wing and male-surstylus geometric morphometrics, genetic and ecological analyses. As a result, a new species is recognised, Chrysotoxum montanum Nedeljković & Vujić sp. nov., and C. vernale is re-defined. A lectotype and paralectotypes are designated for C. vernale to stabilize this concept. An additional species, Chrysotoxum orthostylum Vujić sp. nov., with distinctive male genitalia is also described. The three species share an antenna with the basoflagellomere shorter than the scape plus pedicel and terga with yellow fasciae not reaching the lateral margins. This study confirms the value of integrative approach for resolving species boundaries.

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Integrative approach reveals two new species of Obama (Platyhelminthes: Tricladida) from the South-Brazilian Atlantic Forest

Zootaxa ◽

10.11646/zootaxa.4455.1.4 ◽

2018 ◽

Vol 4455 (1) ◽

pp. 99 ◽

Cited By ~ 2

Author(s):

ALESSANDRO MARQUES ◽

ILANA ROSSI ◽

VICTOR HUGO VALIATI ◽

ANA MARIA LEAL-ZANCHET

Keyword(s):

New Species ◽

Atlantic Forest ◽

Deciduous Forest ◽

Phylogenetic Analyses ◽

Colour Pattern ◽

Integrative Approach ◽

Species Differentiation ◽

Araucaria Forest ◽

Two New Species ◽

Gene Coi

The genus Obama Carbayo et al., 2013 includes 38 species, being the most species-rich within Geoplaninae. Species of this genus show a similar anatomy regarding their copulatory apparatus, which may hinder species differentiation. In this study, we describe two new species, presenting a marbled colour pattern, found in two different phytophysionomies of the Atlantic Forest, namely Semi-deciduous Forest and Araucaria Forest. Both species can be distinguished from their congeners, as well from each other, by colour pattern and eye arrangement combined with characteristics of the pharynx, penis papilla and prostatic vesicle, confirmed by molecular analyses from cytochrome c oxidase subunit I gene (COI). Phylogenetic analyses suggest that both species herein studied are closely related to another species that occurs in areas of Araucaria Forest (O. maculipunctata). Results also indicate the need to use at least 600 bp of the gene COI in the definitions of interspecific divergences and for species delineation, at least for the genus Obama.

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Two new species of marine flatworm from southern China facilitate determination of the phylogenetic position of the genus Nerpa Marcus, 1948 and the histochemical structure of the nervous system in the genus Paucumara Sluys, 1989 (Platyhelminthes, Tricladida, Maricola)

Zootaxa ◽

10.11646/zootaxa.4568.1.9 ◽

2019 ◽

Vol 4568 (1) ◽

pp. 149 ◽

Cited By ~ 2

Author(s):

JIA-JIA CHEN ◽

WEI-XUAN LI ◽

RONALD SLUYS ◽

MING-QI WU ◽

LEI WANG ◽

...

Keyword(s):

Nervous System ◽

New Species ◽

Southern China ◽

Dorsal Side ◽

The Body ◽

Integrative Approach ◽

Phylogenetic Position ◽

Nerve Structure ◽

Two New Species ◽

Copulatory Position

Two new species of flatworm, collected from a beach at eastern Shenzhen, China, were studied through an integrative approach by combining morphological, histological, histochemical (acetylcholinesterase, AChE), and molecular (18S r- DNA) data. These species belong to two genera of marine triclads, previously unrecorded from China, viz. Nerpa Marcus, 1948 and Paucumara Sluys, 1989. Nerpa fistulata Wang & Chen, sp. nov. is characterized by: transparent body; principally pentamerous intestine with three distinct commissures; two very large, prepharyngeal testis follicles; a semi-circular lens in each eye cup; a penis papilla provided with a chitinized, pointed stylet; lateral bursae communicating with the oviduct and opening ventrally to the exterior via a duct. Phylogenetically N. fistulata groups with one member of the family Bdellouridae. This new, Chinese species of Nerpa introduces a major geographic disjunction, as the type species N. evelinae was described from the bay of Santos, Brazil, so that the genus is now known from both Atlantic as well as Pacific coasts. The species Paucumara falcata Wang & Li, sp. nov. is characterized by: three distinct pale yellow transverse pigmentation bands on its dorsal side, between which some snowflake-like specks are randomly distributed, and a brown transverse band anteriorly to the eyes; 8–11 testicular follicles on either side of the body, the follicles extending from immediately behind the ovaries to half-way along the pharyngeal pocket; a musculo-parenchymatic organ with a sclerotic, curved tip projecting from the anterior wall of the male atrium, ventrally to the root of the penis papilla. Phylogenetically P. falcata groups with its congener P. trigonocephala, with the genus Paucumara forming the sister taxon of the genus Ectoplana. Comparison of the nerve structure of P. falcata, as revealed by AChE histochemistry, with that of eight other species of triclad suggested that the nervous system of marine planarians is simpler than that of species of freshwater planarians, but revealed also that the nerve structure is rather variable among species. The copulatory position exhibited by two partners in Paucumara falcata is remarkable in that they intertwine, with their heads pointing downwards and the tails pointing upwards, the entire process lasting about 10 min. Such a copulatory position has never before been reported for triclad flatworms.

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Botanical novelties from the Sierra de Maigualida, southern Venezuela III: revision and two new species of the genus Ilex (Aquifoliaceae)

Anales del Jardín Botánico de Madrid ◽

10.3989/ajbm.2507 ◽

2019 ◽

Vol 76 (1) ◽

pp. 082

Author(s):

José Ramón Grande Allende

Keyword(s):

New Species ◽

Species Delimitation ◽

Mountain Range ◽

Two New Species ◽

Guayana Shield ◽

First Time

The Sierra de Maigualida is a poorly explored mountain range in the central Guayana Shield with high levels of endemism. In the present installment, this area is further delimited and mapped, toponymy is reviewed, and the genus Ilex L. is locally reviewed, with two species, namely I. huberi J.R.Grande sp. nov. and I. maigualidensis J.R.Grande sp. nov., described as new to science. Ilex huberi sp. nov. can be distinguished by its leaf blades strongly revolute, mucronate, and with obscure secondary venation, simple dichasia, and depressed-lunate sepals; I. maigualidensis sp. nov. by its dull leaves, flowers with undeveloped styles, and capitate-subcoronate stigmata. Stem buds and cataphylls are proposed as useful characters in species delimitation within the genus, while I. magnifructa Edwin is illustrated for the first time. A key is presented to differentiate all the species of Ilex of the Sierra de Maigualida.

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Two new species of the genus Crangonyx Bate, 1859 (Amphipoda: Crangonyctidae) from the St. Marks River Basin with notes on the “Crangonyx floridanus complex”

Zootaxa ◽

10.11646/zootaxa.4691.4.1 ◽

2019 ◽

Vol 4691 (4) ◽

pp. 301-332 ◽

Cited By ~ 1

Author(s):

ANDREW G. CANNIZZARO ◽

THOMAS R. SAWICKI

Keyword(s):

New Species ◽

Related Species ◽

Species Delimitation ◽

Species Complex ◽

Morphological Characteristics ◽

Molecular Data ◽

Closely Related Species ◽

Leon County ◽

Two New Species ◽

Morphological And Molecular Data

Crangonyx ephemerus n. sp. and Crangonyx pseudoephemerus n. sp. are described from the headwaters of the St. Marks River in Leon County, Florida, based on detailed morphological and molecular comparisons with the closely related species Crangonyx floridanus Bousfield, 1963. The morphological and molecular data, including three species delimitation models, lend support to the hypothesis that the taxon C. floridanus sensu lato represents a species complex. Diagnostic morphological characteristics are highlighted and discussed within this group to assist in future morphological analyses.

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A multigene and morphological analysis expands the diversity of the seabod shrimp Xiphopenaeus Smith, 1869 (Decapoda: Penaeidae), with descriptions of two new species

Scientific Reports ◽

10.1038/s41598-019-51484-3 ◽

2019 ◽

Vol 9 (1) ◽

Author(s):

Abner Carvalho-Batista ◽

Mariana Terossi ◽

Fernando J. Zara ◽

Fernando L. Mantelatto ◽

Rogerio C. Costa

Keyword(s):

New Species ◽

Morphological Analysis ◽

Taxonomic Status ◽

Morphological Characters ◽

Integrative Approach ◽

Genetic Groups ◽

The Pacific ◽

Two New Species ◽

The Status ◽

History Of

Abstract After being stable for nearly a century, the taxonomic history of the genus Xiphopenaeus has been marked by many changes in the last three decades. The taxonomic status of the Atlantic species has a low resolution, and many species are still undefined and grouped as cryptic species. Here we employed an integrative approach to define the species of Xiphopenaeus and the morphological characters needed to differentiate them. We combined the analyses of two molecular markers (COI and 16 S rDNA), scanning electron microscopy and light microscopy. Based on specimens from 17 localities from the Atlantic and Pacific oceans, we detected five divergent genetic groups, three in the Atlantic (A1, A2, A3) and two in the Pacific (P1, P2). Male secondary sexual characters were able to differentiate four out of the five genetic groups. Group A1 corresponds to X. kroyeri, and A2 and A3 correspond to new species. We redescribed the genus and two new species are described and illustrated: Xiphopenaeus dincao nov. sp. (A2) and Xiphopenaeus baueri nov. sp. (A3). Since the holotype of X. riveti was missing and the specimen analysed from group P2 was a female, the status of the species of Xiphopenaeus from the Pacific remains unresolved.

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Species delimitation of Lachlania Hagen (Ephemeroptera: Oligoneuriidae) with description of two new species from Brazil

Zoosymposia ◽

10.11646/zoosymposia.11.1.14 ◽

2016 ◽

Vol 11 ◽

pp. 121-134 ◽

Cited By ~ 2

Author(s):

FABIANA CRISTE MASSARIOL ◽

ROBERTA PARESQUE ◽

FREDERICO FALCÃO SALLES

Keyword(s):

New Species ◽

Cytochrome Oxidase ◽

Dna Barcoding ◽

Body Length ◽

Species Delimitation ◽

Immature Stages ◽

Dna Barcodes ◽

Cytochrome Oxidase Subunit ◽

Male And Female ◽

Two New Species

Based on DNA barcodes (cytochrome oxidase subunit I sequences) of Lachlania specimens from Atlantic Forest and Cerrado of Brazil, eight lineages were delimited through DNA barcoding and imagoes and immature stages were associated. Among these lineages, two new species are described, Lachlania alcidesi sp. nov. and Lachlania aldinae sp. nov., from State of Espírito Santo. The former species based on nymph, male, and female imagoes, and the latter based on nymph and female imagoes. The main characteristics used to distinguish female imagoes of the new species from those of known species of the genus are: 1) body length; 2) head coloration; 3) number of cross veins on forewing; 4) coloration of caudal filaments; 5) relation between length from point of origin of IMP to margin and total length of MA2- MP1 of forewing.

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Guidelines for DNA barcoding of coralline algae, focusing on Lithophylloideae (Corallinales) from Brazil

Botanica Marina ◽

10.1515/bot-2017-0040 ◽

2018 ◽

Vol 61 (2) ◽

pp. 127-140 ◽

Cited By ~ 5

Author(s):

Beatriz N. Torrano-Silva ◽

Bruno R. Vieira ◽

Rafael Riosmena-Rodríguez ◽

Mariana C. Oliveira

Keyword(s):

New Species ◽

Species Delimitation ◽

Morphological Traits ◽

Dna Barcode ◽

Molecular Data ◽

Coralline Algae ◽

Integrative Approach ◽

Interspecific Divergence ◽

Algorithmic Approaches ◽

Barcode Gap

AbstractMultiple-marker (COI-5P, UPA,psbA andrbcL-3P) and two algorithmic approaches [automatic barcode gap discovery (ABGD) and Poisson tree process (PTP)] were used for species delimitation of Lithophylloideae in Brazil. The integrative approach was mostly congruent between markers and algorithmic methods of species delimitation, suggesting the occurrence of 24 species. Based on morphology and molecular data,Amphiroa rigida,Amphiroa vanbosseae,Lithophyllum atlanticum,Lithophyllum kaiseri,Lithophyllum margaritae,Titanoderma pustulatum,Titanoderma prototypumandPaulsilvella huveorum, which were previously reported for Brazil, are confirmed in this work. Six new species are distinguished by both molecular and morphological traits, and they are provisionally named asAmphiroasp. 1,Amphiroasp. 2,Amphiroasp. 3,Lithophyllumsp. 1,Lithophyllumsp. 2 andLithophyllumsp. 3. Another 10 species are cryptic and cannot be distinguished based on traditionally used morphological traits. These includeAmphiroasp. 4,Lithophyllumsp. 4, three species that are morphologically named underAmphiroa beauvoisii, and six that share the morphology described forAmphiroa fragilissima. All four markers used were useful for species delimitation. However, a combination of practical aspects and levels of intra- and interspecific divergence values led us to propose the use ofrbcL-3P as a standard DNA barcode marker for the Corallinales.

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