Optimization and Antifungal Activity Determination of Tea Tree Oil Containing Oxiconazole Loaded Ufasomes Gel Against Candida Albicans

Introduction: The effectiveness of antimicrobial solutions employed in dental prosthesis decontamination is still uncertain. Aim: To evaluate the antifungal activity of cleaners used in the decontamination of dental prostheses on the growth of Candida albicans. Material and method: The evaluated products were: Corega Tabs(r) (S1), Sodium Hypochlorite 1% (S2), Sodium Bicarbonate 1% (S3), Hydrogen Peroxide 1% (S4), Chlorhexidine Digluconate 0.12% - Periogard (r) (S5), Mouthrinse based on essential oils - Listerine(r) (S6), essential oil from Rosmarinus officinalis (rosemary) at concentrations of 1% (S7) and 2% (S8). The antifungal activity of the products was evaluated by agar diffusion technique and the determination of microbial death curve of samples of C. albicans (ATCC 90028) in concentration 1.5 × 106 CFU/mL. The tests were performed in triplicate and statistical analysis was made by ANOVA Two-Way and Tukey tests, with the confidence level of 95%. Result: The average of the zones of inhibition growth, in millimeters, obtained for the products were: 0.0 (S1), 44.7 (S2), 0.0 (S3), 21.6 (S4), 10.0 (S5), 6.1 (S6), 0.0 (S7) and 2.4 (S8). Considering the determination of microbial death curve, all products showed a statistical difference (p<0.01) from control (0.85% sodium chloride) and S3 groups. Fungal growth less than 2×104 CFU/mL and an accentuation of the microbial death curve were observed after 30 minutes, with exception for S3 and control groups. Conclusion: The studied compounds, with the exception of Sodium Bicarbonate, have antifungal effect against C. albicans, which contribute for dental prostheses hygiene.

Download Full-text

DEVELOPMENT AND VALIDATION OF A NEW RP HPLC METHOD FOR ROUTINE DETERMINATION OF TEA TREE OIL IN BULK AND COSMECEUTICAL FORMULATIONS

INDIAN DRUGS ◽

10.53879/id.56.07.11802 ◽

2019 ◽

Vol 56 (07) ◽

pp. 43-49

Author(s):

B.P. Manjula ◽

V. G Joshi ◽

Siddamsetty Ramachandra Setty ◽

M Geetha ◽

Keyword(s):

High Performance ◽

High Performance Liquid Chromatographic ◽

Reversed Phase ◽

Hplc Method ◽

Tea Tree Oil ◽

Tea Tree ◽

Liquid Chromatographic Method ◽

Development And Validation ◽

Liquid Chromatographic

Tea tree oil, an active ingredient of skin, hair and nail care cosmeceuticals, has claims for topical antimicrobial, analgesic and anti-inflammatory activity. Its complex composition is governed by ISO 4730:2017. Terpinene-4-ol is the principal constituent of the oil (35% - 48%) followed by γ-terpinene (14% -28%), α-terpinene (6%-12%) and 1,8-cineole (≤15%). A reversed-phase, isocratic high performance liquid chromatographic method has been developed and validated for routine determination of tea tree oil based on1,8-cineole content in bulk and commercially available cosmeceuticals using C18 column, methanol-water (70:30 v/v) as mobile phase and flow rate of 1mL/min. UV detection was done at 200 nm. Linearity of the method was established for 20-100μL/mL (R2 = 0.9992) with LOD, LOQ values of 0.5594 μL/mL and 5.5941μL/mL respectively. The % RSD values for robustness and precision were <1% and recovery ranged between 99.09-102.96%. The method was successfully applied for determination of 1,8-cineole content in cosmeceuticals.

Download Full-text

Antimicrobial efficacy of silver ions in combination with tea tree oil against Pseudomonas aeruginosa, Staphylococcus aureus and Candida albicans

International Journal of Antimicrobial Agents ◽

10.1016/j.ijantimicag.2010.10.015 ◽

2011 ◽

Vol 37 (2) ◽

pp. 162-165 ◽

Cited By ~ 33

Author(s):

W.L. Low ◽

C. Martin ◽

D.J. Hill ◽

M.A. Kenward

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Candida Albicans ◽

Silver Ions ◽

Antimicrobial Efficacy ◽

Tea Tree Oil ◽

Tea Tree

Download Full-text

Comparative efficacy of tea tree oil nanoemulgel and clove oil nanoemulgel against Candida albicans

International Journal of Pharmaceutical Investigation ◽

10.4103/jphi.jphi_14_18 ◽

2018 ◽

Vol 8 (1) ◽

pp. 50

Author(s):

JasjeetKaur Narang ◽

RamandeepSingh Narang ◽

Balwinder Singh ◽

SukhdeepS Kahlon ◽

Jiji George ◽

...

Keyword(s):

Candida Albicans ◽

Clove Oil ◽

Tea Tree Oil ◽

Comparative Efficacy ◽

Tea Tree

Download Full-text

Formulation and in vitro characterization of tea tree oil anti-dandruff shampoo

Current Cosmetic Science ◽

10.2174/2666779701666210426085302 ◽

2021 ◽

Vol 01 ◽

Author(s):

Humra Umar ◽

Tariq Mahmood ◽

Talib Hussain ◽

Rabia Aslam ◽

Yasser Shahzad ◽

...

Keyword(s):

Salicylic Acid ◽

Antifungal Activity ◽

Synergistic Effects ◽

Quality Attributes ◽

Tea Tree Oil ◽

Tea Tree ◽

In Vitro Characterization ◽

Selenium Sulfide

Background: Dandruff is the common scalp condition affecting half of the population of the world. Objective: The current study was aimed at developing anti-dandruff shampoos containing tea tree oil, which is believed to have effects against Malassezia furfur, a fungus involved in dandruff production. Methodology: Various shampoos containing tea tree oil in 0.5 to 3% concentration were prepared after careful selection of various shampoo ingredients. The formulated shampoos were subjected to various quality tests such as pH, viscosity, foam production, dirt dispersion, wetting time, surface tension, solid contents and antimicrobial activity against a model fungal strain, namely Candida albicans. The formulated shampoos were also compared with the marketed shampoos for quality attributes. Results: The results revealed that tea tree oil shampoos had pH values in the range of 5 – 6, which is close to the slightly acidic skin’s pH and considered as good for hair. All other quality attributes were comparable to the marketed products. The marketed shampoos had superior antifungal activity due to presence of zinc pyrithione or higher concentration of salicylic acid or selenium sulfide. Notwithstanding, the tea tree oil shampoos demonstrated an appreciable antifungal activity due to synergistic effects of tea tree oil, sodium lauryl sulphate and salicylic acid. Furthermore, the tea tree oil shampoos were stable during two months long stability testing. Conclusion: Thus, tea tree oil anti-dandruff shampoos have the potential to address the dandruff problem.

Download Full-text